Input and runtime details for job 5774122 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5774122 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:30:38 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:16:08 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 23:32:42 CET 2018
	Post-Processing Finished		@ Fri Feb 16 23:32:50 CET 2018
	Job Completed		@ Fri Feb 16 23:33:10 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5774122&toolName=CopraRNA ( 30 days expiry )

Description of the job

FsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000964 Bacillus subtilis subsp. subtilis str. 168

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
171	0.01646	0.3152	bsu04650	endB	-11.87	0.110785	167 -- 193	29 -- 53	antitoxin EndoAI	_____________166_________________________194\n_____________\|___________________________\|\n5'-UUC...AAGAG___U___CGUAA____________U__AUGUU...GAA-3'\n______________UCG_AUC_____GUUUGGUGGAGG_GU\n______________\|\|\|_\|\|\|_____:\|:\|\|:\|:\|\|\|\|_\|\|\n______________AGC_UAG_____UAGACUAUCUCC_CA\n3'-UUU...AAAAA___C___AAC______________C__ACCCC...AUA-5'\n_____________\|___________________________\|\n_____________54__________________________28\n	184 -- 190	32 -- 38	-19.891 kcal/mol	7.03825 kcal/mol	0.985603 kcal/mol	166 194@LB@ \| \|@LB@5'-UUC...AAGAG U CGUAA U AUGUU...GAA-3'@LB@ UCG AUC GUUUGGUGGAGG GU@LB@ \|\|\| \|\|\| :\|:\|\|:\|:\|\|\|\| \|\|@LB@ AGC UAG UAGACUAUCUCC CA@LB@3'-UUU...AAAAA C AAC C ACCCC...AUA-5'@LB@ \| \|@LB@ 54 28@LB@@LB@@LB@Energy -11.87 kcal/mol@LB@Hybridization Energy -19.891 kcal/mol@LB@Unfolding Energy - mRNA 7.03825 kcal/mol@LB@Unfolding Energy - sRNA 0.985603 kcal/mol@LB@@LB@Position - mRNA 167 -- 193@LB@Position - sRNA 29 -- 53@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 32 -- 38@LB@
172	0.01654	0.3152	bsu29680	acsA	-11.36	0.133506	267 -- 288	32 -- 52	acetyl-CoA synthetase	_____________266____________________289\n_____________\|______________________\|\n5'-UUU...CGUAC___C_________G__CC____CAGAG...AUU-3'\n______________CGG_AUUUUGAUU_GG__GAGG\n______________\|\|\|_\|\|:\|\|\|\|\|:_\|:__\|\|\|\|\n______________GCC_UAGAACUAG_CU__CUCC\n3'-UUU...AAAAA_____________A__AU____CCAAC...AUA-5'\n_____________\|______________________\|\n_____________53_____________________31\n	273 -- 279	41 -- 47	-18.8322 kcal/mol	6.49419 kcal/mol	0.976384 kcal/mol	266 289@LB@ \| \|@LB@5'-UUU...CGUAC C G CC CAGAG...AUU-3'@LB@ CGG AUUUUGAUU GG GAGG@LB@ \|\|\| \|\|:\|\|\|\|\|: \|: \|\|\|\|@LB@ GCC UAGAACUAG CU CUCC@LB@3'-UUU...AAAAA A AU CCAAC...AUA-5'@LB@ \| \|@LB@ 53 31@LB@@LB@@LB@Energy -11.36 kcal/mol@LB@Hybridization Energy -18.8322 kcal/mol@LB@Unfolding Energy - mRNA 6.49419 kcal/mol@LB@Unfolding Energy - sRNA 0.976384 kcal/mol@LB@@LB@Position - mRNA 267 -- 288@LB@Position - sRNA 32 -- 52@LB@Position Seed - mRNA 273 -- 279@LB@Position Seed - sRNA 41 -- 47@LB@
173	0.01667	0.3152	bsu28970	ytxB	-13.17	0.066474	182 -- 201	13 -- 34	tvp38/tmem64 family membrane protein YtxB	_____________181____________________202\n_____________\|______________________\|\n5'-UGC...UUCAA_____AA_______U_______UGAAA...AAA-3'\n______________AGGGG____GGAAC_GAGGGUA\n______________\|\|\|\|\|____\|\|\|\|\|_\|\|\|:\|\|\|\n______________UCCCC____CCUUG_CUCUCAU\n3'-UUU...CUAUC_____AACC_____U_______CGAAG...AUA-5'\n_____________\|______________________\|\n_____________35_____________________12\n	195 -- 201	13 -- 19	-25.067 kcal/mol	2.85387 kcal/mol	9.04315 kcal/mol	181 202@LB@ \| \|@LB@5'-UGC...UUCAA AA U UGAAA...AAA-3'@LB@ AGGGG GGAAC GAGGGUA@LB@ \|\|\|\|\| \|\|\|\|\| \|\|\|:\|\|\|@LB@ UCCCC CCUUG CUCUCAU@LB@3'-UUU...CUAUC AACC U CGAAG...AUA-5'@LB@ \| \|@LB@ 35 12@LB@@LB@@LB@Energy -13.17 kcal/mol@LB@Hybridization Energy -25.067 kcal/mol@LB@Unfolding Energy - mRNA 2.85387 kcal/mol@LB@Unfolding Energy - sRNA 9.04315 kcal/mol@LB@@LB@Position - mRNA 182 -- 201@LB@Position - sRNA 13 -- 34@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 13 -- 19@LB@
174	0.01668	0.3152	bsu22210	yprB	-13.05	0.069799	168 -- 191	31 -- 53	'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type pe: enzyme'	_____________167______________________192\n_____________\|________________________\|\n5'-AUU...AGCUG_______AAAU___GUAA______AGGCC...AUG-3'\n______________UUGGAUC____GUC____GGAGGG\n______________\|:\|\|\|\|\|____:\|\|____:\|\|\|\|\|\n______________AGCCUAG____UAG____UCUCCC\n3'-UUU...AAAAA_______AAC____ACUA______CAACC...AUA-5'\n_____________\|________________________\|\n_____________54_______________________30\n	168 -- 174	47 -- 53	-20.3327 kcal/mol	6.30079 kcal/mol	0.981784 kcal/mol	167 192@LB@ \| \|@LB@5'-AUU...AGCUG AAAU GUAA AGGCC...AUG-3'@LB@ UUGGAUC GUC GGAGGG@LB@ \|:\|\|\|\|\| :\|\| :\|\|\|\|\|@LB@ AGCCUAG UAG UCUCCC@LB@3'-UUU...AAAAA AAC ACUA CAACC...AUA-5'@LB@ \| \|@LB@ 54 30@LB@@LB@@LB@Energy -13.05 kcal/mol@LB@Hybridization Energy -20.3327 kcal/mol@LB@Unfolding Energy - mRNA 6.30079 kcal/mol@LB@Unfolding Energy - sRNA 0.981784 kcal/mol@LB@@LB@Position - mRNA 168 -- 191@LB@Position - sRNA 31 -- 53@LB@Position Seed - mRNA 168 -- 174@LB@Position Seed - sRNA 47 -- 53@LB@
175	0.01672	0.3152	bsu04630	ydcC	-11.64	0.120416	196 -- 203	27 -- 34	sporulation protein YdcC	_____________195______204\n_____________\|________\|\n5'-CCG...GAAAA________AAAAA...UGG-3'\n______________AGGGGUUG\n______________\|\|\|\|\|\|\|\|\n______________UCCCCAAC\n3'-UUU...CUAUC________CCCUU...AUA-5'\n_____________\|________\|\n_____________35_______26\n	197 -- 203	27 -- 33	-14.5305 kcal/mol	2.76844 kcal/mol	0.118612 kcal/mol	195 204@LB@ \| \|@LB@5'-CCG...GAAAA AAAAA...UGG-3'@LB@ AGGGGUUG@LB@ \|\|\|\|\|\|\|\|@LB@ UCCCCAAC@LB@3'-UUU...CUAUC CCCUU...AUA-5'@LB@ \| \|@LB@ 35 26@LB@@LB@@LB@Energy -11.64 kcal/mol@LB@Hybridization Energy -14.5305 kcal/mol@LB@Unfolding Energy - mRNA 2.76844 kcal/mol@LB@Unfolding Energy - sRNA 0.118612 kcal/mol@LB@@LB@Position - mRNA 196 -- 203@LB@Position - sRNA 27 -- 34@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 27 -- 33@LB@
176	0.01673	0.3152	bsu08790	thiC	-9.56	0.245346	273 -- 280	31 -- 38	phosphomethylpyrimidine synthase	_____________272______281\n_____________\|________\|\n5'-GGC...UAUAC________UUCUU...AAG-3'\n______________GUGGAGGG\n______________:\|:\|\|\|\|\|\n______________UAUCUCCC\n3'-UUU...UAGAC________CAACC...AUA-5'\n_____________\|________\|\n_____________39_______30\n	274 -- 280	31 -- 37	-12.4867 kcal/mol	2.26991 kcal/mol	0.65488 kcal/mol	272 281@LB@ \| \|@LB@5'-GGC...UAUAC UUCUU...AAG-3'@LB@ GUGGAGGG@LB@ :\|:\|\|\|\|\|@LB@ UAUCUCCC@LB@3'-UUU...UAGAC CAACC...AUA-5'@LB@ \| \|@LB@ 39 30@LB@@LB@@LB@Energy -9.56 kcal/mol@LB@Hybridization Energy -12.4867 kcal/mol@LB@Unfolding Energy - mRNA 2.26991 kcal/mol@LB@Unfolding Energy - sRNA 0.65488 kcal/mol@LB@@LB@Position - mRNA 273 -- 280@LB@Position - sRNA 31 -- 38@LB@Position Seed - mRNA 274 -- 280@LB@Position Seed - sRNA 31 -- 37@LB@
177	0.01681	0.3152	bsu00910	ispF	-14.14	0.044127	219 -- 247	31 -- 51	2-D-methyl-D-erythritol 2 4-cyclodiphosphate synthase	_____________218____________________________248\n_____________\|______________________________\|\n5'-UCG...GACAA__________G__CAUCAAUUA________CCGUC...UUG-3'\n______________GGAUU_UGAU_UG_________GUGGAGGG\n______________\|\|\|\|:_\|\|\|\|_\|\|_________:\|:\|\|\|\|\|\n______________CCUAG_ACUA_AC_________UAUCUCCC\n3'-UUU...AAAAG_____A____G___________________CAACC...AUA-5'\n_____________\|______________________________\|\n_____________52_____________________________30\n	241 -- 247	31 -- 37	-21.3343 kcal/mol	6.22033 kcal/mol	0.970589 kcal/mol	218 248@LB@ \| \|@LB@5'-UCG...GACAA G CAUCAAUUA CCGUC...UUG-3'@LB@ GGAUU UGAU UG GUGGAGGG@LB@ \|\|\|\|: \|\|\|\| \|\| :\|:\|\|\|\|\|@LB@ CCUAG ACUA AC UAUCUCCC@LB@3'-UUU...AAAAG A G CAACC...AUA-5'@LB@ \| \|@LB@ 52 30@LB@@LB@@LB@Energy -14.14 kcal/mol@LB@Hybridization Energy -21.3343 kcal/mol@LB@Unfolding Energy - mRNA 6.22033 kcal/mol@LB@Unfolding Energy - sRNA 0.970589 kcal/mol@LB@@LB@Position - mRNA 219 -- 247@LB@Position - sRNA 31 -- 51@LB@Position Seed - mRNA 241 -- 247@LB@Position Seed - sRNA 31 -- 37@LB@
178	0.01684	0.3152	bsu09460	ctrA	-17.20	0.010320	182 -- 213	20 -- 50	amino acid permease	_____________181_________________________________214\n_____________\|___________________________________\|\n5'-CAA...UAGAU____GG__G____________AA__AAA_______UUUUU...UAA-3'\n______________GAUC__GA_UUGA___AGGGG__UG___GGGAGCG\n______________\|\|\|\|__\|\|_:\|\|\|___\|\|\|\|\|__\|\|___\|\|\|\|:\|:\n______________CUAG__CU_GACU___UCCCC__AC___CCCUUGU\n3'-UUU...AAAGC____AA__A____AUC_____A_____________CUCUC...AUA-5'\n_____________\|___________________________________\|\n_____________51__________________________________19\n	207 -- 213	20 -- 26	-25.5851 kcal/mol	4.15406 kcal/mol	4.22897 kcal/mol	181 214@LB@ \| \|@LB@5'-CAA...UAGAU GG G AA AAA UUUUU...UAA-3'@LB@ GAUC GA UUGA AGGGG UG GGGAGCG@LB@ \|\|\|\| \|\| :\|\|\| \|\|\|\|\| \|\| \|\|\|\|:\|:@LB@ CUAG CU GACU UCCCC AC CCCUUGU@LB@3'-UUU...AAAGC AA A AUC A CUCUC...AUA-5'@LB@ \| \|@LB@ 51 19@LB@@LB@@LB@Energy -17.20 kcal/mol@LB@Hybridization Energy -25.5851 kcal/mol@LB@Unfolding Energy - mRNA 4.15406 kcal/mol@LB@Unfolding Energy - sRNA 4.22897 kcal/mol@LB@@LB@Position - mRNA 182 -- 213@LB@Position - sRNA 20 -- 50@LB@Position Seed - mRNA 207 -- 213@LB@Position Seed - sRNA 20 -- 26@LB@
179	0.01703	0.3152	bsu08425	mprF	-11.09	0.146985	133 -- 167	22 -- 52	phosphatidylglycerol lysyltransferase	_____________132__________________________________168\n_____________\|____________________________________\|\n5'-ACU...AAUAA___UAG___UAUAA___GU_____AUUA________GAGGA...AUC-3'\n______________CGG____UG_____UGA__UGGAG____UUGGGGGA\n______________\|\|\|____\|\|_____\|\|\|__\|:\|\|\|____\|\|\|\|\|\|:\|\n______________GCC____AC_____ACU__AUCUC____AACCCCUU\n3'-UUU...AAAAA___UAGA__UAG____________CCC_________GUCUC...AUA-5'\n_____________\|____________________________________\|\n_____________53___________________________________21\n	161 -- 167	22 -- 28	-20.3953 kcal/mol	7.42087 kcal/mol	1.8808 kcal/mol	132 168@LB@ \| \|@LB@5'-ACU...AAUAA UAG UAUAA GU AUUA GAGGA...AUC-3'@LB@ CGG UG UGA UGGAG UUGGGGGA@LB@ \|\|\| \|\| \|\|\| \|:\|\|\| \|\|\|\|\|\|:\|@LB@ GCC AC ACU AUCUC AACCCCUU@LB@3'-UUU...AAAAA UAGA UAG CCC GUCUC...AUA-5'@LB@ \| \|@LB@ 53 21@LB@@LB@@LB@Energy -11.09 kcal/mol@LB@Hybridization Energy -20.3953 kcal/mol@LB@Unfolding Energy - mRNA 7.42087 kcal/mol@LB@Unfolding Energy - sRNA 1.8808 kcal/mol@LB@@LB@Position - mRNA 133 -- 167@LB@Position - sRNA 22 -- 52@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 22 -- 28@LB@
180	0.01704	0.3152	bsu28380	gerM	-9.54	0.246988	269 -- 291	32 -- 52	spore germination protein GerM	_____________268______________________292\n_____________\|________________________\|\n5'-AGU...GGAUG___UUU____CA____________CAGCC...AGG-3'\n______________CGG___GUUU__AUCUGAUA_AGG\n______________\|\|\|___:\|:\|__\|\|\|\|\|\|\|\|_\|\|\|\n______________GCC___UAGA__UAGACUAU_UCC\n3'-UUU...AAAAA__________AC________C___CCAAC...AUA-5'\n_____________\|________________________\|\n_____________53_______________________31\n	282 -- 288	36 -- 42	-15.6565 kcal/mol	5.13957 kcal/mol	0.976384 kcal/mol	268 292@LB@ \| \|@LB@5'-AGU...GGAUG UUU CA CAGCC...AGG-3'@LB@ CGG GUUU AUCUGAUA AGG@LB@ \|\|\| :\|:\| \|\|\|\|\|\|\|\| \|\|\|@LB@ GCC UAGA UAGACUAU UCC@LB@3'-UUU...AAAAA AC C CCAAC...AUA-5'@LB@ \| \|@LB@ 53 31@LB@@LB@@LB@Energy -9.54 kcal/mol@LB@Hybridization Energy -15.6565 kcal/mol@LB@Unfolding Energy - mRNA 5.13957 kcal/mol@LB@Unfolding Energy - sRNA 0.976384 kcal/mol@LB@@LB@Position - mRNA 269 -- 291@LB@Position - sRNA 32 -- 52@LB@Position Seed - mRNA 282 -- 288@LB@Position Seed - sRNA 36 -- 42@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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