| 151 |
0.01347 |
0.2966 |
bsu25570 |
comEC |
-9.44 |
0.254651 |
59 -- 89 |
9 -- 50 |
ComE operon protein 3 |
|
_____________58__________________________________________90\n_____________|___________________________________________|\n5'-CGC...GAAAU____G_________AA____________________AA_____UCAUU...UCU-3'\n______________GAUC_UUGAUCUGA______________AACAGAGA__AGCUU\n______________||||_|||||||||______________||||||||__|||||\n______________CUAG_AACUAGACU______________UUGUCUCU__UCGAA\n3'-UUU...AAAGC______________AUCUCCCCAACCCC________CA_____GAGAGAUA-5'\n_____________|___________________________________________|\n_____________51__________________________________________8\n |
76 -- 82 |
16 -- 22 |
-28.9501 kcal/mol |
9.55134 kcal/mol |
9.9565 kcal/mol |
58 90@LB@ | |@LB@5'-CGC...GAAAU G AA AA UCAUU...UCU-3'@LB@ GAUC UUGAUCUGA AACAGAGA AGCUU@LB@ |||| ||||||||| |||||||| |||||@LB@ CUAG AACUAGACU UUGUCUCU UCGAA@LB@3'-UUU...AAAGC AUCUCCCCAACCCC CA GAGAGAUA-5'@LB@ | |@LB@ 51 8@LB@@LB@@LB@Energy -9.44 kcal/mol@LB@Hybridization Energy -28.9501 kcal/mol@LB@Unfolding Energy - mRNA 9.55134 kcal/mol@LB@Unfolding Energy - sRNA 9.9565 kcal/mol@LB@@LB@Position - mRNA 59 -- 89@LB@Position - sRNA 9 -- 50@LB@Position Seed - mRNA 76 -- 82@LB@Position Seed - sRNA 16 -- 22@LB@ |
| 152 |
0.01351 |
0.2966 |
bsu25600 |
comER |
-9.59 |
0.243237 |
173 -- 194 |
30 -- 51 |
ComE operon protein 4 |
|
_____________172____________________195\n_____________|______________________|\n5'-AGC...UUAGA____AAUAGCUCAAA_______AAACC...CAA-3'\n______________GGAU___________GGAGGGG\n______________||||___________:||||||\n______________CCUA___________UCUCCCC\n3'-UUU...AAAAG____GAACUAGACUA_______AACCC...AUA-5'\n_____________|______________________|\n_____________52_____________________29\n |
188 -- 194 |
30 -- 36 |
-16.7526 kcal/mol |
6.18889 kcal/mol |
0.974282 kcal/mol |
172 195@LB@ | |@LB@5'-AGC...UUAGA AAUAGCUCAAA AAACC...CAA-3'@LB@ GGAU GGAGGGG@LB@ |||| :||||||@LB@ CCUA UCUCCCC@LB@3'-UUU...AAAAG GAACUAGACUA AACCC...AUA-5'@LB@ | |@LB@ 52 29@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -16.7526 kcal/mol@LB@Unfolding Energy - mRNA 6.18889 kcal/mol@LB@Unfolding Energy - sRNA 0.974282 kcal/mol@LB@@LB@Position - mRNA 173 -- 194@LB@Position - sRNA 30 -- 51@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 153 |
0.01377 |
0.2994 |
bsu37510 |
pbpG |
-15.25 |
0.026855 |
95 -- 111 |
23 -- 38 |
penicillin-binding protein 2D |
|
_____________94_________________112\n_____________|__________________|\n5'-UGU...AAAGU_______AU__C______AAAAG...UGU-3'\n______________AUAGAGG__GG__GGGGA\n______________|||||||__||__|||||\n______________UAUCUCC__CC__CCCCU\n3'-UUU...UAGAC___________AA_____UGUCU...AUA-5'\n_____________|__________________|\n_____________39_________________22\n |
95 -- 101 |
32 -- 38 |
-18.2895 kcal/mol |
2.3676 kcal/mol |
0.66977 kcal/mol |
94 112@LB@ | |@LB@5'-UGU...AAAGU AU C AAAAG...UGU-3'@LB@ AUAGAGG GG GGGGA@LB@ ||||||| || |||||@LB@ UAUCUCC CC CCCCU@LB@3'-UUU...UAGAC AA UGUCU...AUA-5'@LB@ | |@LB@ 39 22@LB@@LB@@LB@Energy -15.25 kcal/mol@LB@Hybridization Energy -18.2895 kcal/mol@LB@Unfolding Energy - mRNA 2.3676 kcal/mol@LB@Unfolding Energy - sRNA 0.66977 kcal/mol@LB@@LB@Position - mRNA 95 -- 111@LB@Position - sRNA 23 -- 38@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 154 |
0.01388 |
0.2994 |
bsu23480 |
dacF |
-14.01 |
0.046761 |
175 -- 192 |
31 -- 51 |
D-alanyl-D-alanine carboxypeptidase DacF |
|
_____________174___________________193\n_____________|_____________________|\n5'-UGG...AAAAA_____A___A___________CUUUU...UUG-3'\n______________GGAUU_UGA____AUGGAGGG\n______________||||:_|||____||:|||||\n______________CCUAG_ACU____UAUCUCCC\n3'-UUU...AAAAG_____A___AGAC________CAACC...AUA-5'\n_____________|_____________________|\n_____________52____________________30\n |
186 -- 192 |
31 -- 37 |
-18.482 kcal/mol |
3.50235 kcal/mol |
0.970589 kcal/mol |
174 193@LB@ | |@LB@5'-UGG...AAAAA A A CUUUU...UUG-3'@LB@ GGAUU UGA AUGGAGGG@LB@ ||||: ||| ||:|||||@LB@ CCUAG ACU UAUCUCCC@LB@3'-UUU...AAAAG A AGAC CAACC...AUA-5'@LB@ | |@LB@ 52 30@LB@@LB@@LB@Energy -14.01 kcal/mol@LB@Hybridization Energy -18.482 kcal/mol@LB@Unfolding Energy - mRNA 3.50235 kcal/mol@LB@Unfolding Energy - sRNA 0.970589 kcal/mol@LB@@LB@Position - mRNA 175 -- 192@LB@Position - sRNA 31 -- 51@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 155 |
0.01391 |
0.2994 |
bsu24780 |
yqgY |
-10.29 |
0.193646 |
188 -- 194 |
30 -- 36 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________187_____195\n_____________|_______|\n5'-UGU...UCUCG_______AUAAA...GAC-3'\n______________GGAGGGG\n______________:||||||\n______________UCUCCCC\n3'-UUU...GACUA_______AACCC...AUA-5'\n_____________|_______|\n_____________37______29\n |
188 -- 194 |
30 -- 36 |
-13.7207 kcal/mol |
3.29345 kcal/mol |
0.133643 kcal/mol |
187 195@LB@ | |@LB@5'-UGU...UCUCG AUAAA...GAC-3'@LB@ GGAGGGG@LB@ :||||||@LB@ UCUCCCC@LB@3'-UUU...GACUA AACCC...AUA-5'@LB@ | |@LB@ 37 29@LB@@LB@@LB@Energy -10.29 kcal/mol@LB@Hybridization Energy -13.7207 kcal/mol@LB@Unfolding Energy - mRNA 3.29345 kcal/mol@LB@Unfolding Energy - sRNA 0.133643 kcal/mol@LB@@LB@Position - mRNA 188 -- 194@LB@Position - sRNA 30 -- 36@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 30 -- 36@LB@ |
| 156 |
0.0141 |
0.3008 |
bsu23600 |
yqxK |
-15.33 |
0.025853 |
66 -- 91 |
32 -- 51 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________65_________________________92\n_____________|__________________________|\n5'-GAA...GAUGA___CGAGUU_G____AG_________UGACG...GAA-3'\n______________GGA______U_UUGA__UGAUGGAGG\n______________|||______|_||||__||||:||||\n______________CCU______A_AACU__ACUAUCUCC\n3'-UUU...AAAAG__________G____AG_________CCAAC...AUA-5'\n_____________|__________________________|\n_____________52_________________________31\n |
85 -- 91 |
32 -- 38 |
-19.9693 kcal/mol |
3.66503 kcal/mol |
0.97021 kcal/mol |
65 92@LB@ | |@LB@5'-GAA...GAUGA CGAGUU G AG UGACG...GAA-3'@LB@ GGA U UUGA UGAUGGAGG@LB@ ||| | |||| ||||:||||@LB@ CCU A AACU ACUAUCUCC@LB@3'-UUU...AAAAG G AG CCAAC...AUA-5'@LB@ | |@LB@ 52 31@LB@@LB@@LB@Energy -15.33 kcal/mol@LB@Hybridization Energy -19.9693 kcal/mol@LB@Unfolding Energy - mRNA 3.66503 kcal/mol@LB@Unfolding Energy - sRNA 0.97021 kcal/mol@LB@@LB@Position - mRNA 66 -- 91@LB@Position - sRNA 32 -- 51@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 157 |
0.01433 |
0.3008 |
bsu38150 |
qoxC |
-14.65 |
0.035334 |
274 -- 297 |
30 -- 53 |
quinol oxidase subunit 3 |
|
_____________273_______________________298\n_____________|_________________________|\n5'-ACC...UAUUC__________G____UUUU______CAG-3'\n______________UCGGGUUUUG_AUCU_____AGGGG\n______________||||:|:|||_||||_____|||||\n______________AGCCUAGAAC_UAGA_____UCCCC\n3'-UUU...AAAAA_______________CUAUC_____AACCC...AUA-5'\n_____________|_________________________|\n_____________54________________________29\n |
277 -- 283 |
44 -- 50 |
-24.1532 kcal/mol |
8.5194 kcal/mol |
0.985344 kcal/mol |
273 298@LB@ | |@LB@5'-ACC...UAUUC G UUUU CAG-3'@LB@ UCGGGUUUUG AUCU AGGGG@LB@ ||||:|:||| |||| |||||@LB@ AGCCUAGAAC UAGA UCCCC@LB@3'-UUU...AAAAA CUAUC AACCC...AUA-5'@LB@ | |@LB@ 54 29@LB@@LB@@LB@Energy -14.65 kcal/mol@LB@Hybridization Energy -24.1532 kcal/mol@LB@Unfolding Energy - mRNA 8.5194 kcal/mol@LB@Unfolding Energy - sRNA 0.985344 kcal/mol@LB@@LB@Position - mRNA 274 -- 297@LB@Position - sRNA 30 -- 53@LB@Position Seed - mRNA 277 -- 283@LB@Position Seed - sRNA 44 -- 50@LB@ |
| 158 |
0.01436 |
0.3008 |
bsu10130 |
hemH |
-12.69 |
0.080628 |
178 -- 227 |
6 -- 44 |
ferrochelatase |
|
_____________177____________________________________________________228\n_____________|______________________________________________________|\n5'-GAG...UAUCA____AUUAA______U__A_________U____AGAAAGAAAAUGG________GUGAU...GAA-3'\n______________GAUC_____AGAGGG_GU_____AACAG_GAGU_____________GGCUUCUC\n______________||||_____||||||_||_____|||||_||||_____________:|||||||\n______________CUAG_____UCUCCC_CA_____UUGUC_CUCA_____________UCGAAGAG\n3'-UUU...UAGAA____ACUA__________ACCCC_____U_________________________AGAUA-5'\n_____________|______________________________________________________|\n_____________45_____________________________________________________5\n |
220 -- 226 |
7 -- 13 |
-32.766 kcal/mol |
10.122 kcal/mol |
9.95356 kcal/mol |
177 228@LB@ | |@LB@5'-GAG...UAUCA AUUAA U A U AGAAAGAAAAUGG GUGAU...GAA-3'@LB@ GAUC AGAGGG GU AACAG GAGU GGCUUCUC@LB@ |||| |||||| || ||||| |||| :|||||||@LB@ CUAG UCUCCC CA UUGUC CUCA UCGAAGAG@LB@3'-UUU...UAGAA ACUA ACCCC U AGAUA-5'@LB@ | |@LB@ 45 5@LB@@LB@@LB@Energy -12.69 kcal/mol@LB@Hybridization Energy -32.766 kcal/mol@LB@Unfolding Energy - mRNA 10.122 kcal/mol@LB@Unfolding Energy - sRNA 9.95356 kcal/mol@LB@@LB@Position - mRNA 178 -- 227@LB@Position - sRNA 6 -- 44@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 7 -- 13@LB@ |
| 159 |
0.01439 |
0.3008 |
bsu00560 |
spoVT |
-10.85 |
0.160189 |
242 -- 254 |
41 -- 53 |
stage V sporulation protein T |
|
_____________241___________255\n_____________|_____________|\n5'-ACA...UUAGG_______G_____CCUAA...CGC-3'\n______________UCGGGUC_UGAUU\n______________||||:||_||||:\n______________AGCCUAG_ACUAG\n3'-UUU...AAAAA_______A_____ACUAU...AUA-5'\n_____________|_____________|\n_____________54____________40\n |
242 -- 248 |
47 -- 53 |
-14.0147 kcal/mol |
2.18551 kcal/mol |
0.979862 kcal/mol |
241 255@LB@ | |@LB@5'-ACA...UUAGG G CCUAA...CGC-3'@LB@ UCGGGUC UGAUU@LB@ ||||:|| ||||:@LB@ AGCCUAG ACUAG@LB@3'-UUU...AAAAA A ACUAU...AUA-5'@LB@ | |@LB@ 54 40@LB@@LB@@LB@Energy -10.85 kcal/mol@LB@Hybridization Energy -14.0147 kcal/mol@LB@Unfolding Energy - mRNA 2.18551 kcal/mol@LB@Unfolding Energy - sRNA 0.979862 kcal/mol@LB@@LB@Position - mRNA 242 -- 254@LB@Position - sRNA 41 -- 53@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 160 |
0.01451 |
0.3008 |
bsu30080 |
yteV |
-11.71 |
0.117611 |
279 -- 298 |
34 -- 53 |
sporulation protein cse60 |
|
_____________278__________________299\n_____________|____________________|\n5'-UUU...GGAUA_____GGAGCAG________UA-3'\n______________UCGGA_______CUGAUAGA\n______________|||||_______||||||||\n______________AGCCU_______GACUAUCU\n3'-UUU...AAAAA_____AGAACUA________CCCCA...AUA-5'\n_____________|____________________|\n_____________54___________________33\n |
292 -- 298 |
34 -- 40 |
-18.1644 kcal/mol |
5.47565 kcal/mol |
0.98171 kcal/mol |
278 299@LB@ | |@LB@5'-UUU...GGAUA GGAGCAG UA-3'@LB@ UCGGA CUGAUAGA@LB@ ||||| ||||||||@LB@ AGCCU GACUAUCU@LB@3'-UUU...AAAAA AGAACUA CCCCA...AUA-5'@LB@ | |@LB@ 54 33@LB@@LB@@LB@Energy -11.71 kcal/mol@LB@Hybridization Energy -18.1644 kcal/mol@LB@Unfolding Energy - mRNA 5.47565 kcal/mol@LB@Unfolding Energy - sRNA 0.98171 kcal/mol@LB@@LB@Position - mRNA 279 -- 298@LB@Position - sRNA 34 -- 53@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 34 -- 40@LB@ |
