Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
70	0.01838	0.6733	lmo0265	N/A	-16.33	0.055331	156 -- 199	158 -- 207	succinyl-diaminopimelate desuccinylase	_____________155_____200\n_____________\|_______\|\n5'-UUU...UAGAG_______AAACA...UGU-3'\n______________AACGGGA\n______________\|\|\|\|\|\|\|\n______________UUGCCCU\n3'-GUU...ACGUA_______ACUCC...GGA-5'\n_____________\|_______\|\n_____________208_____157\n	156 -- 162	201 -- 207	-11.1784 kcal/mol	0.573427 kcal/mol	0.326294 kcal/mol	155 200@LB@ \| \|@LB@5'-UUU...UAGAG AAACA...UGU-3'@LB@ AACGGGA@LB@ \|\|\|\|\|\|\|@LB@ UUGCCCU@LB@3'-GUU...ACGUA ACUCC...GGA-5'@LB@ \| \|@LB@ 208 157@LB@@LB@@LB@Energy -16.33 kcal/mol@LB@Hybridization Energy -11.1784 kcal/mol@LB@Unfolding Energy - mRNA 0.573427 kcal/mol@LB@Unfolding Energy - sRNA 0.326294 kcal/mol@LB@@LB@Position - mRNA 156 -- 199@LB@Position - sRNA 158 -- 207@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 201 -- 207@LB@
169	0.04649	0.7403	lmo0268	N/A	-16.22	0.057928	275 -- 284	195 -- 204	phosphoglycerate mutase	_____________274________285\n_____________\|__________\|\n5'-ACC...AUGCA__________GAUUC...UUG-3'\n______________GGGAUGGGGC\n______________\|\|\|\|\|\|:\|\|\|\n______________CCCUACUCCG\n3'-GUU...UAUUG__________UAUCU...GGA-5'\n_____________\|__________\|\n_____________205________194\n	278 -- 284	195 -- 201	-19.94 kcal/mol	1.96731 kcal/mol	6.55531 kcal/mol	274 285@LB@ \| \|@LB@5'-ACC...AUGCA GAUUC...UUG-3'@LB@ GGGAUGGGGC@LB@ \|\|\|\|\|\|:\|\|\|@LB@ CCCUACUCCG@LB@3'-GUU...UAUUG UAUCU...GGA-5'@LB@ \| \|@LB@ 205 194@LB@@LB@@LB@Energy -16.22 kcal/mol@LB@Hybridization Energy -19.94 kcal/mol@LB@Unfolding Energy - mRNA 1.96731 kcal/mol@LB@Unfolding Energy - sRNA 6.55531 kcal/mol@LB@@LB@Position - mRNA 275 -- 284@LB@Position - sRNA 195 -- 204@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 195 -- 201@LB@
15	0.003165	0.4469	lmo0292	N/A	-21.41	0.004857	182 -- 249	155 -- 212	heat-shock protein htrA serine protease	_____________181_____________________________________________________________250\n_____________\|_______________________________________________________________\|\n5'-GAG...GCAGA____AAGGA__________G_G_AAGAAAAGAAUUUA_____AAACAGC______________CGCCG...AAC-3'\n______________GAGG_____AGACAUGGAC_A_A______________AAUGA_______GA_GAAUGAAAGCA\n______________\|\|\|\|_____\|\|\|\|\|\|\|:\|\|_\|_\|______________\|\|\|\|\|_______\|\|_\|\|\|\|\|\|\|\|:\|\|\n______________CUCC_____UCUGUACUUG_U_U______________UUACU_______CU_CUUACUUUUGU\n3'-GUU...CCCUA____GUA____________A_G_GCCGG_______________________A___________UUAUU...GGA-5'\n_____________\|_______________________________________________________________\|\n_____________213_____________________________________________________________154\n	243 -- 249	155 -- 161	-30.3296 kcal/mol	4.19045 kcal/mol	13.9822 kcal/mol	181 250@LB@ \| \|@LB@5'-GAG...GCAGA AAGGA G G AAGAAAAGAAUUUA AAACAGC CGCCG...AAC-3'@LB@ GAGG AGACAUGGAC A A AAUGA GA GAAUGAAAGCA@LB@ \|\|\|\| \|\|\|\|\|\|\|:\|\| \| \| \|\|\|\|\| \|\| \|\|\|\|\|\|\|\|:\|\|@LB@ CUCC UCUGUACUUG U U UUACU CU CUUACUUUUGU@LB@3'-GUU...CCCUA GUA A G GCCGG A UUAUU...GGA-5'@LB@ \| \|@LB@ 213 154@LB@@LB@@LB@Energy -21.41 kcal/mol@LB@Hybridization Energy -30.3296 kcal/mol@LB@Unfolding Energy - mRNA 4.19045 kcal/mol@LB@Unfolding Energy - sRNA 13.9822 kcal/mol@LB@@LB@Position - mRNA 182 -- 249@LB@Position - sRNA 155 -- 212@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 155 -- 161@LB@
52	0.01176	0.599	lmo0302	N/A	-19.32	0.013869	143 -- 287	107 -- 220	lmo0302	_____________142________________288\n_____________\|__________________\|\n5'-CGA...CAUAG_________A__U_____AAAAA...AUA-3'\n______________GAGAUGAAU_GA_GAUGA\n______________\|\|\|\|\|\|\|\|\|_\|\|_:\|::\|\n______________CUCUACUUA_CU_UUGUU\n3'-GUU...GGUUA____________U_____UAUUA...GGA-5'\n_____________\|__________________\|\n_____________221________________106\n	189 -- 195	162 -- 168	-14.2485 kcal/mol	1.08239 kcal/mol	0.933796 kcal/mol	142 288@LB@ \| \|@LB@5'-CGA...CAUAG A U AAAAA...AUA-3'@LB@ GAGAUGAAU GA GAUGA@LB@ \|\|\|\|\|\|\|\|\| \|\| :\|::\|@LB@ CUCUACUUA CU UUGUU@LB@3'-GUU...GGUUA U UAUUA...GGA-5'@LB@ \| \|@LB@ 221 106@LB@@LB@@LB@Energy -19.32 kcal/mol@LB@Hybridization Energy -14.2485 kcal/mol@LB@Unfolding Energy - mRNA 1.08239 kcal/mol@LB@Unfolding Energy - sRNA 0.933796 kcal/mol@LB@@LB@Position - mRNA 143 -- 287@LB@Position - sRNA 107 -- 220@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 162 -- 168@LB@
19	0.003536	0.4469	lmo0321	N/A	-22.74	0.002408	114 -- 157	167 -- 207		_____________113____________________________________________158\n_____________\|______________________________________________\|\n5'-AUU...UGUUA________UA____G_AC__________GGA_____U_________AUGUU...AGG-3'\n______________AGCGGGAU__GAGG_A__UAGGUA_GAA____ACGG_CUAAUGAGA\n______________\|:\|\|\|\|\|\|__\|\|\|\|_\|__\|\|\|::\|_\|\|\|____\|\|\|\|_\|:\|\|\|\|\|\|\|\n______________UUGCCCUA__CUCC_U__AUCUGU_CUU____UGCC_GGUUACUCU\n3'-GUU...ACGUA______________G_________A___GAUG______________ACUUA...GGA-5'\n_____________\|______________________________________________\|\n_____________208____________________________________________166\n	151 -- 157	167 -- 173	-39.7249 kcal/mol	9.73371 kcal/mol	18.0488 kcal/mol	113 158@LB@ \| \|@LB@5'-AUU...UGUUA UA G AC GGA U AUGUU...AGG-3'@LB@ AGCGGGAU GAGG A UAGGUA GAA ACGG CUAAUGAGA@LB@ \|:\|\|\|\|\|\| \|\|\|\| \| \|\|\|::\| \|\|\| \|\|\|\| \|:\|\|\|\|\|\|\|@LB@ UUGCCCUA CUCC U AUCUGU CUU UGCC GGUUACUCU@LB@3'-GUU...ACGUA G A GAUG ACUUA...GGA-5'@LB@ \| \|@LB@ 208 166@LB@@LB@@LB@Energy -22.74 kcal/mol@LB@Hybridization Energy -39.7249 kcal/mol@LB@Unfolding Energy - mRNA 9.73371 kcal/mol@LB@Unfolding Energy - sRNA 18.0488 kcal/mol@LB@@LB@Position - mRNA 114 -- 157@LB@Position - sRNA 167 -- 207@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 167 -- 173@LB@
43	0.009411	0.5889	lmo0325	N/A	-15.65	0.074138	261 -- 289	174 -- 199	transcriptional regulator	______________261_________________________290\n______________\|___________________________\|\n___________5'-_______AAUACUUAAAAAA________ACGAU...UCG-3'\n______________UGCUAUU_____________GGAAGAUG\n______________\|\|\|:\|\|\|_____________\|\|\|\|\|\|\|\|\n______________ACGGUAA_____________CCUUCUAC\n3'-GUU...GAUCA_______GAUAA________________CUAUG...GGA-5'\n_____________\|____________________________\|\n_____________200__________________________173\n	22 -- 28	97 -- 103	-16.3216 kcal/mol	2.64423 kcal/mol	5.20765 kcal/mol	261 290@LB@ \| \|@LB@ 5'- AAUACUUAAAAAA ACGAU...UCG-3'@LB@ UGCUAUU GGAAGAUG@LB@ \|\|\|:\|\|\| \|\|\|\|\|\|\|\|@LB@ ACGGUAA CCUUCUAC@LB@3'-GUU...GAUCA GAUAA CUAUG...GGA-5'@LB@ \| \|@LB@ 200 173@LB@@LB@@LB@Energy -15.65 kcal/mol@LB@Hybridization Energy -16.3216 kcal/mol@LB@Unfolding Energy - mRNA 2.64423 kcal/mol@LB@Unfolding Energy - sRNA 5.20765 kcal/mol@LB@@LB@Position - mRNA 261 -- 289@LB@Position - sRNA 174 -- 199@LB@Position Seed - mRNA 22 -- 28@LB@Position Seed - sRNA 97 -- 103@LB@
67	0.01689	0.6733	lmo0335	N/A	-16.81	0.044620	154 -- 214	153 -- 220	lmo0335	_____________153_____________________________________________________________215\n_____________\|_______________________________________________________________\|\n5'-AAA...CUACC______AUA______G____A____AUAUA______G_G____________A___________AUAAA...AUA-3'\n______________GGUGCU_____AUGA_GGGG__AGG_____CAUAGA_A_GAA__GCAU_GC_GAUGAGA_GAA\n______________\|\|:\|\|:_____\|\|:\|_\|\|\|:__\|\|\|_____\|\|\|\|\|\|_\|_\|\|\|__:\|\|:_\|\|_:\|\|\|\|\|\|_\|\|\|\n______________CCGCGG_____UAUU_CCCU__UCC_____GUAUCU_U_CUU__UGUG_CG_UUACUCU_CUU\n3'-GUU...CAAGC______AUACG____G____AC______________G_A___GA____C__G_______A___ACUUU...GGA-5'\n_____________\|_______________________________________________________________\|\n_____________221_____________________________________________________________152\n	200 -- 206	167 -- 173	-43.0643 kcal/mol	10.4394 kcal/mol	21.4092 kcal/mol	153 215@LB@ \| \|@LB@5'-AAA...CUACC AUA G A AUAUA G G A AUAAA...AUA-3'@LB@ GGUGCU AUGA GGGG AGG CAUAGA A GAA GCAU GC GAUGAGA GAA@LB@ \|\|:\|\|: \|\|:\| \|\|\|: \|\|\| \|\|\|\|\|\| \| \|\|\| :\|\|: \|\| :\|\|\|\|\|\| \|\|\|@LB@ CCGCGG UAUU CCCU UCC GUAUCU U CUU UGUG CG UUACUCU CUU@LB@3'-GUU...CAAGC AUACG G AC G A GA C G A ACUUU...GGA-5'@LB@ \| \|@LB@ 221 152@LB@@LB@@LB@Energy -16.81 kcal/mol@LB@Hybridization Energy -43.0643 kcal/mol@LB@Unfolding Energy - mRNA 10.4394 kcal/mol@LB@Unfolding Energy - sRNA 21.4092 kcal/mol@LB@@LB@Position - mRNA 154 -- 214@LB@Position - sRNA 153 -- 220@LB@Position Seed - mRNA 200 -- 206@LB@Position Seed - sRNA 167 -- 173@LB@
197	0.05638	0.7607	lmo0344	N/A	-15.66	0.073858	267 -- 298	182 -- 211	short chain dehydrogenase	_____________266______299\n_____________\|________\|\n5'-CAG...CAGCA________UGGUA...UUU-3'\n______________AGCGGGAU\n______________\|:\|\|\|\|\|\|\n______________UUGCCCUA\n3'-GUU...ACGUA________CUCCG...GGA-5'\n_____________\|________\|\n_____________212______181\n	271 -- 277	200 -- 206	-11.8444 kcal/mol	1.0403 kcal/mol	0.330969 kcal/mol	266 299@LB@ \| \|@LB@5'-CAG...CAGCA UGGUA...UUU-3'@LB@ AGCGGGAU@LB@ \|:\|\|\|\|\|\|@LB@ UUGCCCUA@LB@3'-GUU...ACGUA CUCCG...GGA-5'@LB@ \| \|@LB@ 212 181@LB@@LB@@LB@Energy -15.66 kcal/mol@LB@Hybridization Energy -11.8444 kcal/mol@LB@Unfolding Energy - mRNA 1.0403 kcal/mol@LB@Unfolding Energy - sRNA 0.330969 kcal/mol@LB@@LB@Position - mRNA 267 -- 298@LB@Position - sRNA 182 -- 211@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 200 -- 206@LB@
105	0.02711	0.6733	lmo0347	N/A	-16.41	0.053410	62 -- 87	160 -- 186	dihydroxyacetone kinase	_____________61____________________________88\n_____________\|_____________________________\|\n5'-AUC...GUUGG__________ACU________________UUGGU...AUU-3'\n______________GAACUACGCG___UCGAUG_GAU__AUGA\n______________\|\|\|\|\|\|\|:\|\|___:\|:\|\|\|_\|\|\|__\|\|\|\|\n______________CUUGAUGUGC___GGUUAC_CUA__UACU\n3'-GUU...CUGUA__________C________U___CU____UUUGU...GGA-5'\n_____________\|_____________________________\|\n_____________187___________________________159\n	65 -- 71	177 -- 183	-23.7931 kcal/mol	4.23572 kcal/mol	9.57505 kcal/mol	61 88@LB@ \| \|@LB@5'-AUC...GUUGG ACU UUGGU...AUU-3'@LB@ GAACUACGCG UCGAUG GAU AUGA@LB@ \|\|\|\|\|\|\|:\|\| :\|:\|\|\| \|\|\| \|\|\|\|@LB@ CUUGAUGUGC GGUUAC CUA UACU@LB@3'-GUU...CUGUA C U CU UUUGU...GGA-5'@LB@ \| \|@LB@ 187 159@LB@@LB@@LB@Energy -16.41 kcal/mol@LB@Hybridization Energy -23.7931 kcal/mol@LB@Unfolding Energy - mRNA 4.23572 kcal/mol@LB@Unfolding Energy - sRNA 9.57505 kcal/mol@LB@@LB@Position - mRNA 62 -- 87@LB@Position - sRNA 160 -- 186@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 177 -- 183@LB@
120	0.03214	0.6968	lmo0368	N/A	-17.05	0.040150	163 -- 213	95 -- 164		_____________162______________________________________________214\n_____________\|________________________________________________\|\n5'-UGC...AAUGA_____A_____AA_______AGGGA___G______G_______A____AUUUG...UUC-3'\n______________AAGCA___GUC__UGAUUAG_____GCC_AUUUUA_UGGAAGA_UGGG\n______________\|\|:\|\|___\|\|\|__\|\|\|:\|\|\|_____\|\|\|_\|\|\|:\|\|_\|\|\|\|\|\|\|_\|\|\|:\n______________UUUGU___CAG__ACUGAUC_____CGG_UAAGAU_ACCUUCU_ACCU\n3'-GUU...ACAGA_____CUA____________AA_____________A____________AUGUG...GGA-5'\n_____________\|________________________________________________\|\n_____________165______________________________________________94\n	202 -- 208	99 -- 105	-32.1999 kcal/mol	10.9316 kcal/mol	9.49359 kcal/mol	162 214@LB@ \| \|@LB@5'-UGC...AAUGA A AA AGGGA G G A AUUUG...UUC-3'@LB@ AAGCA GUC UGAUUAG GCC AUUUUA UGGAAGA UGGG@LB@ \|\|:\|\| \|\|\| \|\|\|:\|\|\| \|\|\| \|\|\|:\|\| \|\|\|\|\|\|\| \|\|\|:@LB@ UUUGU CAG ACUGAUC CGG UAAGAU ACCUUCU ACCU@LB@3'-GUU...ACAGA CUA AA A AUGUG...GGA-5'@LB@ \| \|@LB@ 165 94@LB@@LB@@LB@Energy -17.05 kcal/mol@LB@Hybridization Energy -32.1999 kcal/mol@LB@Unfolding Energy - mRNA 10.9316 kcal/mol@LB@Unfolding Energy - sRNA 9.49359 kcal/mol@LB@@LB@Position - mRNA 163 -- 213@LB@Position - sRNA 95 -- 164@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 99 -- 105@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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