Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
40	0.00848	0.5705	lmo2399	N/A	-21.52	0.004592	127 -- 195	156 -- 207		_____________126_________________________________________________________________196\n_____________\|___________________________________________________________________\|\n5'-AUU...UCGAC_________GU_______CUUGUGGUUUAAAC____AGA____G__UAGA______A___G______GCUAA...CUG-3'\n______________GGUGAGGCA__GGCG_GA______________GCUA___ACGG_UA____AUGGGG_GAA_GAGGAU\n______________\|:\|\|\|\|\|\|\|__\|:\|:_\|\|______________:\|\|\|___\|\|\|\|_:\|____\|\|\|:\|:_\|\|\|_\|\|::\|:\n______________CUACUCCGU__CUGU_CU______________UGAU___UGCC_GU____UACUCU_CUU_CUUUUG\n3'-GUU...UUGCC_________AU____A____________________G______G____________A___A______UUUAU...GGA-5'\n_____________\|___________________________________________________________________\|\n_____________208_________________________________________________________________155\n	132 -- 138	194 -- 200	-37.8581 kcal/mol	11.6457 kcal/mol	14.014 kcal/mol	126 196@LB@ \| \|@LB@5'-AUU...UCGAC GU CUUGUGGUUUAAAC AGA G UAGA A G GCUAA...CUG-3'@LB@ GGUGAGGCA GGCG GA GCUA ACGG UA AUGGGG GAA GAGGAU@LB@ \|:\|\|\|\|\|\|\| \|:\|: \|\| :\|\|\| \|\|\|\| :\| \|\|\|:\|: \|\|\| \|\|::\|:@LB@ CUACUCCGU CUGU CU UGAU UGCC GU UACUCU CUU CUUUUG@LB@3'-GUU...UUGCC AU A G G A A UUUAU...GGA-5'@LB@ \| \|@LB@ 208 155@LB@@LB@@LB@Energy -21.52 kcal/mol@LB@Hybridization Energy -37.8581 kcal/mol@LB@Unfolding Energy - mRNA 11.6457 kcal/mol@LB@Unfolding Energy - sRNA 14.014 kcal/mol@LB@@LB@Position - mRNA 127 -- 195@LB@Position - sRNA 156 -- 207@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 194 -- 200@LB@
144	0.03753	0.6968	lmo2422	N/A	-16.62	0.048504	29 -- 68	172 -- 209	two-component response regulator	_____________28___________________________________69\n_____________\|____________________________________\|\n5'-AUG...AUUCG____________AAAU_______G___U________UUUAU...AUG-3'\n______________CUAUGU__GACG____UGAGGCA_AGA_UAUGAAUU\n______________\|\|\|\|\|:__:\|\|\|____\|\|\|\|\|\|\|_\|\|\|_:\|\|\|\|\|:\|\n______________GAUACG__UUGC____ACUCCGU_UCU_GUACUUGA\n3'-GUU...CCGCG______UA____CCU________A____________UGUGC...GGA-5'\n_____________\|____________________________________\|\n_____________210__________________________________171\n	39 -- 45	194 -- 200	-29.3631 kcal/mol	6.47642 kcal/mol	13.3074 kcal/mol	28 69@LB@ \| \|@LB@5'-AUG...AUUCG AAAU G U UUUAU...AUG-3'@LB@ CUAUGU GACG UGAGGCA AGA UAUGAAUU@LB@ \|\|\|\|\|: :\|\|\| \|\|\|\|\|\|\| \|\|\| :\|\|\|\|\|:\|@LB@ GAUACG UUGC ACUCCGU UCU GUACUUGA@LB@3'-GUU...CCGCG UA CCU A UGUGC...GGA-5'@LB@ \| \|@LB@ 210 171@LB@@LB@@LB@Energy -16.62 kcal/mol@LB@Hybridization Energy -29.3631 kcal/mol@LB@Unfolding Energy - mRNA 6.47642 kcal/mol@LB@Unfolding Energy - sRNA 13.3074 kcal/mol@LB@@LB@Position - mRNA 29 -- 68@LB@Position - sRNA 172 -- 209@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 194 -- 200@LB@
91	0.02389	0.6733	lmo2425	N/A	-16.15	0.059838	240 -- 263	146 -- 170	glycine cleavage system protein H	_____________239______________264\n_____________\|________________\|\n5'-CGA...AACAC________________GACGA...AUC-3'\n______________GA_AUGGGUGAAAGCA\n______________\|\|_\|\|\|::\|\|\|\|\|:\|\|\n______________CU_UACUUACUUUUGU\n3'-GUU...GGUUA__C_____________UUAUU...GGA-5'\n_____________\|________________\|\n_____________171______________145\n	248 -- 254	155 -- 161	-15.1065 kcal/mol	3.08859 kcal/mol	0.929544 kcal/mol	239 264@LB@ \| \|@LB@5'-CGA...AACAC GACGA...AUC-3'@LB@ GA AUGGGUGAAAGCA@LB@ \|\| \|\|\|::\|\|\|\|\|:\|\|@LB@ CU UACUUACUUUUGU@LB@3'-GUU...GGUUA C UUAUU...GGA-5'@LB@ \| \|@LB@ 171 145@LB@@LB@@LB@Energy -16.15 kcal/mol@LB@Hybridization Energy -15.1065 kcal/mol@LB@Unfolding Energy - mRNA 3.08859 kcal/mol@LB@Unfolding Energy - sRNA 0.929544 kcal/mol@LB@@LB@Position - mRNA 240 -- 263@LB@Position - sRNA 146 -- 170@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 155 -- 161@LB@
95	0.02474	0.6733	lmo2446	N/A	-18.44	0.021161	178 -- 278	95 -- 188	glycosidase	_____________177____________________279\n_____________\|______________________\|\n5'-UGA...AGGAA___________GC_A_______GAAAA...ACG-3'\n______________AGUGAGAUGAA__G_AGAACGA\n______________\|:\|\|\|\|\|\|\|\|\|__\|_\|:\|\|\|:\|\n______________UUACUCUACUU__C_UUUUGUU\n3'-GUU...GCCGG___________A__________UAUUA...GGA-5'\n_____________\|______________________\|\n_____________189____________________94\n	210 -- 216	154 -- 160	-17.9823 kcal/mol	4.45349 kcal/mol	0.959215 kcal/mol	177 279@LB@ \| \|@LB@5'-UGA...AGGAA GC A GAAAA...ACG-3'@LB@ AGUGAGAUGAA G AGAACGA@LB@ \|:\|\|\|\|\|\|\|\|\| \| \|:\|\|\|:\|@LB@ UUACUCUACUU C UUUUGUU@LB@3'-GUU...GCCGG A UAUUA...GGA-5'@LB@ \| \|@LB@ 189 94@LB@@LB@@LB@Energy -18.44 kcal/mol@LB@Hybridization Energy -17.9823 kcal/mol@LB@Unfolding Energy - mRNA 4.45349 kcal/mol@LB@Unfolding Energy - sRNA 0.959215 kcal/mol@LB@@LB@Position - mRNA 178 -- 278@LB@Position - sRNA 95 -- 188@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 154 -- 160@LB@
119	0.03213	0.6968	lmo2454	N/A	-17.21	0.037384	166 -- 217	153 -- 209	lmo2454	_____________165_____________218\n_____________\|_______________\|\n5'-GUG...GACGC__U____C_______AAGCC...AAA-3'\n______________UG_GGUG_AUGAAAA\n______________\|\|_\|:\|\|_\|\|\|\|\|\|\|\n______________AC_CUAC_UACUUUU\n3'-GUU...CGGUU__U____U_______GUUUA...GGA-5'\n_____________\|_______________\|\n_____________210_____________152\n	274 -- 280	157 -- 163	-10.6243 kcal/mol	2.77636 kcal/mol	0.905109 kcal/mol	165 218@LB@ \| \|@LB@5'-GUG...GACGC U C AAGCC...AAA-3'@LB@ UG GGUG AUGAAAA@LB@ \|\| \|:\|\| \|\|\|\|\|\|\|@LB@ AC CUAC UACUUUU@LB@3'-GUU...CGGUU U U GUUUA...GGA-5'@LB@ \| \|@LB@ 210 152@LB@@LB@@LB@Energy -17.21 kcal/mol@LB@Hybridization Energy -10.6243 kcal/mol@LB@Unfolding Energy - mRNA 2.77636 kcal/mol@LB@Unfolding Energy - sRNA 0.905109 kcal/mol@LB@@LB@Position - mRNA 166 -- 217@LB@Position - sRNA 153 -- 209@LB@Position Seed - mRNA 274 -- 280@LB@Position Seed - sRNA 157 -- 163@LB@
84	0.02276	0.6733	lmo2467	N/A	-18.10	0.024762	166 -- 213	154 -- 198	chitin-binding protein	_____________165__________________________________________________214\n_____________\|____________________________________________________\|\n5'-UCA...CAAAA_____________________UGAAA__AA____CAUC______________CGAAG...AAC-3'\n______________AGGUAUA___AUGAACUAUAC_____GG__GGUG____AUGAA_GAAAAUGA\n______________\|\|\|:\|\|\|___\|\|\|\|\|\|\|\|:\|\|_____\|\|__::\|\|____\|\|\|\|\|_\|\|\|\|\|::\|\n______________UCCGUAU___UACUUGAUGUG_____CC__UUAC____UACUU_CUUUUGUU\n3'-GUU...CCUAC_______CUG__________________GG____UC_______A________UAUUA...GGA-5'\n_____________\|____________________________________________________\|\n_____________199__________________________________________________153\n	206 -- 212	155 -- 161	-31.7912 kcal/mol	6.75491 kcal/mol	13.9801 kcal/mol	165 214@LB@ \| \|@LB@5'-UCA...CAAAA UGAAA AA CAUC CGAAG...AAC-3'@LB@ AGGUAUA AUGAACUAUAC GG GGUG AUGAA GAAAAUGA@LB@ \|\|\|:\|\|\| \|\|\|\|\|\|\|\|:\|\| \|\| ::\|\| \|\|\|\|\| \|\|\|\|\|::\|@LB@ UCCGUAU UACUUGAUGUG CC UUAC UACUU CUUUUGUU@LB@3'-GUU...CCUAC CUG GG UC A UAUUA...GGA-5'@LB@ \| \|@LB@ 199 153@LB@@LB@@LB@Energy -18.10 kcal/mol@LB@Hybridization Energy -31.7912 kcal/mol@LB@Unfolding Energy - mRNA 6.75491 kcal/mol@LB@Unfolding Energy - sRNA 13.9801 kcal/mol@LB@@LB@Position - mRNA 166 -- 213@LB@Position - sRNA 154 -- 198@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 155 -- 161@LB@
139	0.03644	0.6968	lmo2566	N/A	-16.54	0.050390	198 -- 240	121 -- 170	lmo2566	_____________197_________________241\n_____________\|___________________\|\n5'-GAA...ACAUA_________AUA_______CCUUA...CAG-3'\n______________GAGAUGAAU___GAAAACA\n______________\|\|\|\|\|\|\|\|\|___\|\|\|\|\|\|\|\n______________CUCUACUUA___CUUUUGU\n3'-GUU...GGUUA___________________UUAUU...GGA-5'\n_____________\|___________________\|\n_____________171_________________120\n	210 -- 216	155 -- 161	-17.6586 kcal/mol	1.26524 kcal/mol	0.929544 kcal/mol	197 241@LB@ \| \|@LB@5'-GAA...ACAUA AUA CCUUA...CAG-3'@LB@ GAGAUGAAU GAAAACA@LB@ \|\|\|\|\|\|\|\|\| \|\|\|\|\|\|\|@LB@ CUCUACUUA CUUUUGU@LB@3'-GUU...GGUUA UUAUU...GGA-5'@LB@ \| \|@LB@ 171 120@LB@@LB@@LB@Energy -16.54 kcal/mol@LB@Hybridization Energy -17.6586 kcal/mol@LB@Unfolding Energy - mRNA 1.26524 kcal/mol@LB@Unfolding Energy - sRNA 0.929544 kcal/mol@LB@@LB@Position - mRNA 198 -- 240@LB@Position - sRNA 121 -- 170@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 155 -- 161@LB@
183	0.0534	0.7607	lmo2583	N/A	-16.44	0.052604	178 -- 212	151 -- 181	DNA-binding response regulator	_____________177___________________________213\n_____________\|_____________________________\|\n5'-UAA...AAAUA_G_____AA______C_____________AACAA...AUG-3'\n______________A_ACGGU__GGUGAG___GGUGAAAAUGA\n______________\|_\|\|\|\|:__::\|\|\|\|___::\|\|\|\|\|\|::\|\n______________U_UGCCG__UUACUC___UUACUUUUGUU\n3'-GUU...CUUGA_G_____G_______UAC___________UAUUA...GGA-5'\n_____________\|_____________________________\|\n_____________182___________________________150\n	197 -- 203	155 -- 161	-21.6156 kcal/mol	3.93053 kcal/mol	8.33518 kcal/mol	177 213@LB@ \| \|@LB@5'-UAA...AAAUA G AA C AACAA...AUG-3'@LB@ A ACGGU GGUGAG GGUGAAAAUGA@LB@ \| \|\|\|\|: ::\|\|\|\| ::\|\|\|\|\|\|::\|@LB@ U UGCCG UUACUC UUACUUUUGUU@LB@3'-GUU...CUUGA G G UAC UAUUA...GGA-5'@LB@ \| \|@LB@ 182 150@LB@@LB@@LB@Energy -16.44 kcal/mol@LB@Hybridization Energy -21.6156 kcal/mol@LB@Unfolding Energy - mRNA 3.93053 kcal/mol@LB@Unfolding Energy - sRNA 8.33518 kcal/mol@LB@@LB@Position - mRNA 178 -- 212@LB@Position - sRNA 151 -- 181@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 155 -- 161@LB@
97	0.02539	0.6733	lmo2585	N/A	-13.14	0.201793	84 -- 125	164 -- 211		_____________83______126\n_____________\|_______\|\n5'-ACA...GAUGC_______AAGGG...AAG-3'\n______________GGCGGGA\n______________::\|\|\|\|\|\n______________UUGCCCU\n3'-GUU...ACGUA_______ACUCC...GGA-5'\n_____________\|_______\|\n_____________212_____163\n	178 -- 184	201 -- 207	-10.7547 kcal/mol	3.27081 kcal/mol	0.326294 kcal/mol	83 126@LB@ \| \|@LB@5'-ACA...GAUGC AAGGG...AAG-3'@LB@ GGCGGGA@LB@ ::\|\|\|\|\|@LB@ UUGCCCU@LB@3'-GUU...ACGUA ACUCC...GGA-5'@LB@ \| \|@LB@ 212 163@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -10.7547 kcal/mol@LB@Unfolding Energy - mRNA 3.27081 kcal/mol@LB@Unfolding Energy - sRNA 0.326294 kcal/mol@LB@@LB@Position - mRNA 84 -- 125@LB@Position - sRNA 164 -- 211@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 201 -- 207@LB@
142	0.03693	0.6968	lmo2586	N/A	-16.96	0.041696	196 -- 266	151 -- 227	formate dehydrogenase subunit alpha	_____________195______267\n_____________\|________\|\n5'-UUU...GUGGC________UGGUG...AUC-3'\n______________GCGGGAUG\n______________:\|\|\|\|\|\|\|\n______________UGCCCUAC\n3'-GUU...CGUAU________UCCGU...GGA-5'\n_____________\|________\|\n_____________228______150\n	240 -- 246	199 -- 205	-13.0644 kcal/mol	3.68871 kcal/mol	0.331032 kcal/mol	195 267@LB@ \| \|@LB@5'-UUU...GUGGC UGGUG...AUC-3'@LB@ GCGGGAUG@LB@ :\|\|\|\|\|\|\|@LB@ UGCCCUAC@LB@3'-GUU...CGUAU UCCGU...GGA-5'@LB@ \| \|@LB@ 228 150@LB@@LB@@LB@Energy -16.96 kcal/mol@LB@Hybridization Energy -13.0644 kcal/mol@LB@Unfolding Energy - mRNA 3.68871 kcal/mol@LB@Unfolding Energy - sRNA 0.331032 kcal/mol@LB@@LB@Position - mRNA 196 -- 266@LB@Position - sRNA 151 -- 227@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 199 -- 205@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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