Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
74	0.01955	0.6733	lmo0190	ipk	-17.49	0.032930	174 -- 210	154 -- 192	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase	_____________173_______________________________________211\n_____________\|_________________________________________\|\n5'-AUA...AAAAG____UU_________GA___________AA___________GCAUU...CAA-3'\n______________AGAU__UGAAC_GCG__GGCCA___AGA__AAUGAAAAUAA\n______________\|\|\|:__\|\|\|\|\|_:\|:__\|\|\|\|\|___\|\|\|__\|\|\|\|\|\|\|\|:\|\|\n______________UCUG__ACUUG_UGU__CCGGU___UCU__UUACUUUUGUU\n3'-GUU...CCGUA____U______A___G______UAC___AC___________UAUUA...GGA-5'\n_____________\|_________________________________________\|\n_____________193_______________________________________153\n	204 -- 210	154 -- 160	-28.6344 kcal/mol	5.15958 kcal/mol	12.1365 kcal/mol	173 211@LB@ \| \|@LB@5'-AUA...AAAAG UU GA AA GCAUU...CAA-3'@LB@ AGAU UGAAC GCG GGCCA AGA AAUGAAAAUAA@LB@ \|\|\|: \|\|\|\|\| :\|: \|\|\|\|\| \|\|\| \|\|\|\|\|\|\|\|:\|\|@LB@ UCUG ACUUG UGU CCGGU UCU UUACUUUUGUU@LB@3'-GUU...CCGUA U A G UAC AC UAUUA...GGA-5'@LB@ \| \|@LB@ 193 153@LB@@LB@@LB@Energy -17.49 kcal/mol@LB@Hybridization Energy -28.6344 kcal/mol@LB@Unfolding Energy - mRNA 5.15958 kcal/mol@LB@Unfolding Energy - sRNA 12.1365 kcal/mol@LB@@LB@Position - mRNA 174 -- 210@LB@Position - sRNA 154 -- 192@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 154 -- 160@LB@
112	0.029	0.6968	lmo0191	N/A	-14.99	0.097641	103 -- 160	154 -- 212	phospho-beta-glucosidase	_____________102__________161\n_____________\|____________\|\n5'-UGU...CCAUG____________GAACG...CGC-3'\n______________GAGUGAAGGCGA\n______________\|\|:\|\|\|\|::\|:\|\n______________CUUACUUUUGUU\n3'-GUU...CUCUA____________UAUUA...GGA-5'\n_____________\|____________\|\n_____________213__________153\n	148 -- 154	154 -- 160	-12.5182 kcal/mol	1.90916 kcal/mol	0.857742 kcal/mol	102 161@LB@ \| \|@LB@5'-UGU...CCAUG GAACG...CGC-3'@LB@ GAGUGAAGGCGA@LB@ \|\|:\|\|\|\|::\|:\|@LB@ CUUACUUUUGUU@LB@3'-GUU...CUCUA UAUUA...GGA-5'@LB@ \| \|@LB@ 213 153@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -12.5182 kcal/mol@LB@Unfolding Energy - mRNA 1.90916 kcal/mol@LB@Unfolding Energy - sRNA 0.857742 kcal/mol@LB@@LB@Position - mRNA 103 -- 160@LB@Position - sRNA 154 -- 212@LB@Position Seed - mRNA 148 -- 154@LB@Position Seed - sRNA 154 -- 160@LB@
175	0.04975	0.7607	lmo0194	N/A	-16.29	0.056201	181 -- 242	155 -- 212	ABC transporter ATP-binding protein	_____________180________________________243\n_____________\|__________________________\|\n_______5'-AAGA________AAACGAAAAA________AAUUU...AGC-3'\n______________CAAUGGGA__________AGUGAAGA\n______________\|\|\|\|\|:\|\|__________\|:\|\|\|\|:\|\n______________GUUACUCU__________UUACUUUU\n3'-GUU...UGCCG________AC________________GUUUA...GGA-5'\n_____________\|__________________________\|\n_____________213________________________154\n	24 -- 30	157 -- 163	-10.5893 kcal/mol	0.67181 kcal/mol	0.933292 kcal/mol	180 243@LB@ \| \|@LB@ 5'-AAGA AAACGAAAAA AAUUU...AGC-3'@LB@ CAAUGGGA AGUGAAGA@LB@ \|\|\|\|\|:\|\| \|:\|\|\|\|:\|@LB@ GUUACUCU UUACUUUU@LB@3'-GUU...UGCCG AC GUUUA...GGA-5'@LB@ \| \|@LB@ 213 154@LB@@LB@@LB@Energy -16.29 kcal/mol@LB@Hybridization Energy -10.5893 kcal/mol@LB@Unfolding Energy - mRNA 0.67181 kcal/mol@LB@Unfolding Energy - sRNA 0.933292 kcal/mol@LB@@LB@Position - mRNA 181 -- 242@LB@Position - sRNA 155 -- 212@LB@Position Seed - mRNA 24 -- 30@LB@Position Seed - sRNA 157 -- 163@LB@
3	0.0004111	0.3688	lmo0196	N/A	-23.83	0.001321	198 -- 256	156 -- 209	regulatory protein SpoVG	_____________197________________257\n_____________\|__________________\|\n5'-ACU...AGACA__________________UAUUU...ACA-3'\n______________GAUGGGA_GAAUGAAAGC\n______________:\|\|\|:\|\|_\|\|\|\|\|\|\|\|:\|\n______________UUACUCU_CUUACUUUUG\n3'-GUU...GCCGG_______A__________UUUAU...GGA-5'\n_____________\|__________________\|\n_____________210________________155\n	250 -- 256	156 -- 162	-19.3926 kcal/mol	2.92904 kcal/mol	0.941471 kcal/mol	197 257@LB@ \| \|@LB@5'-ACU...AGACA UAUUU...ACA-3'@LB@ GAUGGGA GAAUGAAAGC@LB@ :\|\|\|:\|\| \|\|\|\|\|\|\|\|:\|@LB@ UUACUCU CUUACUUUUG@LB@3'-GUU...GCCGG A UUUAU...GGA-5'@LB@ \| \|@LB@ 210 155@LB@@LB@@LB@Energy -23.83 kcal/mol@LB@Hybridization Energy -19.3926 kcal/mol@LB@Unfolding Energy - mRNA 2.92904 kcal/mol@LB@Unfolding Energy - sRNA 0.941471 kcal/mol@LB@@LB@Position - mRNA 198 -- 256@LB@Position - sRNA 156 -- 209@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 156 -- 162@LB@
11	0.002745	0.4469	lmo0197	N/A	-21.05	0.005861	175 -- 258	154 -- 211	regulatory protein SpoVG	_____________174__________________259\n_____________\|____________________\|\n5'-UUA...AAACU_______A____________UUGCU...AUA-3'\n______________GAUG_GA_GAAUGAGAGCGA\n______________:\|\|\|_\|\|_\|\|\|\|\|\|:\|:\|:\|\n______________UUAC_CU_CUUACUUUUGUU\n3'-GUU...GCCGG____U__A____________UAUUA...GGA-5'\n_____________\|____________________\|\n_____________212__________________153\n	252 -- 258	154 -- 160	-17.4227 kcal/mol	2.96559 kcal/mol	0.959215 kcal/mol	174 259@LB@ \| \|@LB@5'-UUA...AAACU A UUGCU...AUA-3'@LB@ GAUG GA GAAUGAGAGCGA@LB@ :\|\|\| \|\| \|\|\|\|\|\|:\|:\|:\|@LB@ UUAC CU CUUACUUUUGUU@LB@3'-GUU...GCCGG U A UAUUA...GGA-5'@LB@ \| \|@LB@ 212 153@LB@@LB@@LB@Energy -21.05 kcal/mol@LB@Hybridization Energy -17.4227 kcal/mol@LB@Unfolding Energy - mRNA 2.96559 kcal/mol@LB@Unfolding Energy - sRNA 0.959215 kcal/mol@LB@@LB@Position - mRNA 175 -- 258@LB@Position - sRNA 154 -- 211@LB@Position Seed - mRNA 252 -- 258@LB@Position Seed - sRNA 154 -- 160@LB@
121	0.03234	0.6968	lmo0198	glmU	-15.85	0.067914	228 -- 259	153 -- 184	bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase	_____________227______________________________260\n_____________\|________________________________\|\n5'-UAC...UGCUU_____________GGCACAC________U___AACUU...UGG-3'\n______________GCUGC_UGGCCAA_______GGAUGAAA_CAA\n______________:\|\|:\|_:\|\|\|\|\|\|_______\|:\|\|\|\|\|\|_\|\|\|\n______________UGAUG_GCCGGUU_______CUUACUUU_GUU\n3'-GUU...GUACU_____U_______ACUCUA_________U___UAUUA...GGA-5'\n_____________\|________________________________\|\n_____________185______________________________152\n	248 -- 254	158 -- 164	-25.2592 kcal/mol	7.21839 kcal/mol	9.59579 kcal/mol	227 260@LB@ \| \|@LB@5'-UAC...UGCUU GGCACAC U AACUU...UGG-3'@LB@ GCUGC UGGCCAA GGAUGAAA CAA@LB@ :\|\|:\| :\|\|\|\|\|\| \|:\|\|\|\|\|\| \|\|\|@LB@ UGAUG GCCGGUU CUUACUUU GUU@LB@3'-GUU...GUACU U ACUCUA U UAUUA...GGA-5'@LB@ \| \|@LB@ 185 152@LB@@LB@@LB@Energy -15.85 kcal/mol@LB@Hybridization Energy -25.2592 kcal/mol@LB@Unfolding Energy - mRNA 7.21839 kcal/mol@LB@Unfolding Energy - sRNA 9.59579 kcal/mol@LB@@LB@Position - mRNA 228 -- 259@LB@Position - sRNA 153 -- 184@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 158 -- 164@LB@
82	0.02188	0.6733	lmo0200	prfA	-17.77	0.028863	191 -- 222	162 -- 204	listeriolysin positive regulatory protein	_____________190______223\n_____________\|________\|\n5'-UCA...AUUGG________ACAUG...AUC-3'\n______________GGGAUGAG\n______________\|\|\|\|\|\|\|\|\n______________CCCUACUC\n3'-GUU...UAUUG________CGUAU...GGA-5'\n_____________\|________\|\n_____________205______161\n	192 -- 198	197 -- 203	-14.1607 kcal/mol	1.90446 kcal/mol	0.386531 kcal/mol	190 223@LB@ \| \|@LB@5'-UCA...AUUGG ACAUG...AUC-3'@LB@ GGGAUGAG@LB@ \|\|\|\|\|\|\|\|@LB@ CCCUACUC@LB@3'-GUU...UAUUG CGUAU...GGA-5'@LB@ \| \|@LB@ 205 161@LB@@LB@@LB@Energy -17.77 kcal/mol@LB@Hybridization Energy -14.1607 kcal/mol@LB@Unfolding Energy - mRNA 1.90446 kcal/mol@LB@Unfolding Energy - sRNA 0.386531 kcal/mol@LB@@LB@Position - mRNA 191 -- 222@LB@Position - sRNA 162 -- 204@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 197 -- 203@LB@
178	0.05066	0.7607	lmo0216	N/A	-18.27	0.022949	55 -- 114	166 -- 213		_____________54__________________________________________________________________________________115\n_____________\|___________________________________________________________________________________\|\n5'-GCG...UCCAA__AAU______A______A__A___G__________UUUCCUUGUUUUU___CUA______A______U___U____A_____UUUGU...CGG-3'\n______________GC_____UGUU___GUGC_CU_GUG_GUGGCGGGAU_____________GCA___AGAUAU_AAUUAC_CGG_CCAA__AGAU\n______________\|\|_____\|\|\|\|___\|:\|\|_\|\|_:\|\|_:\|::\|\|\|\|\|\|_____________\|\|\|___\|\|\|:\|\|_\|\|:\|\|\|_\|\|\|_\|\|\|\|__\|\|\|\|\n______________CG_____ACAA___CGCG_GA_UAC_UAUUGCCCUA_____________CGU___UCUGUA_UUGAUG_GCC_GGUU__UCUA\n3'-GUU...CGCAA__GUAGU____GCC___________G__________CUC_____________A________C______U________AC____CUUAC...GGA-5'\n_____________\|___________________________________________________________________________________\|\n_____________214_________________________________________________________________________________165\n	62 -- 68	200 -- 206	-50.1796 kcal/mol	16.7648 kcal/mol	22.1647 kcal/mol	54 115@LB@ \| \|@LB@5'-GCG...UCCAA AAU A A A G UUUCCUUGUUUUU CUA A U U A UUUGU...CGG-3'@LB@ GC UGUU GUGC CU GUG GUGGCGGGAU GCA AGAUAU AAUUAC CGG CCAA AGAU@LB@ \|\| \|\|\|\| \|:\|\| \|\| :\|\| :\|::\|\|\|\|\|\| \|\|\| \|\|\|:\|\| \|\|:\|\|\| \|\|\| \|\|\|\| \|\|\|\|@LB@ CG ACAA CGCG GA UAC UAUUGCCCUA CGU UCUGUA UUGAUG GCC GGUU UCUA@LB@3'-GUU...CGCAA GUAGU GCC G CUC A C U AC CUUAC...GGA-5'@LB@ \| \|@LB@ 214 165@LB@@LB@@LB@Energy -18.27 kcal/mol@LB@Hybridization Energy -50.1796 kcal/mol@LB@Unfolding Energy - mRNA 16.7648 kcal/mol@LB@Unfolding Energy - sRNA 22.1647 kcal/mol@LB@@LB@Position - mRNA 55 -- 114@LB@Position - sRNA 166 -- 213@LB@Position Seed - mRNA 62 -- 68@LB@Position Seed - sRNA 200 -- 206@LB@
113	0.02939	0.6968	lmo0232	clpC	-17.21	0.037249	171 -- 212	159 -- 202	endopeptidase Clp ATP-binding chain C	_____________170___________________________________________213\n_____________\|_____________________________________________\|\n5'-AUU...UAAAU________U__C____A___GGAA__AA___________UU____CGAUU...AUA-3'\n______________GAUGAGGC_UA_GAUA_GGA____AC___CAAUGA__UG__UGGA\n______________\|\|\|\|\|\|\|\|_\|\|_\|\|:\|_\|:\|____\|\|___\|\|\|\|\|\|__\|\|__\|\|:\|\n______________CUACUCCG_AU_CUGU_CUU____UG___GUUACU__AC__ACUU\n3'-GUU...UUGCC________U_______A___GAUG__CCG______CU__UU____UUGUU...GGA-5'\n_____________\|_____________________________________________\|\n_____________203___________________________________________158\n	172 -- 178	195 -- 201	-28.9894 kcal/mol	4.99237 kcal/mol	13.9304 kcal/mol	170 213@LB@ \| \|@LB@5'-AUU...UAAAU U C A GGAA AA UU CGAUU...AUA-3'@LB@ GAUGAGGC UA GAUA GGA AC CAAUGA UG UGGA@LB@ \|\|\|\|\|\|\|\| \|\| \|\|:\| \|:\| \|\| \|\|\|\|\|\| \|\| \|\|:\|@LB@ CUACUCCG AU CUGU CUU UG GUUACU AC ACUU@LB@3'-GUU...UUGCC U A GAUG CCG CU UU UUGUU...GGA-5'@LB@ \| \|@LB@ 203 158@LB@@LB@@LB@Energy -17.21 kcal/mol@LB@Hybridization Energy -28.9894 kcal/mol@LB@Unfolding Energy - mRNA 4.99237 kcal/mol@LB@Unfolding Energy - sRNA 13.9304 kcal/mol@LB@@LB@Position - mRNA 171 -- 212@LB@Position - sRNA 159 -- 202@LB@Position Seed - mRNA 172 -- 178@LB@Position Seed - sRNA 195 -- 201@LB@
13	0.002947	0.4469	lmo0233	N/A	-20.88	0.006381	238 -- 276	156 -- 198	DNA repair protein RadA	_____________237_____________________________________________277\n_____________\|_______________________________________________\|\n5'-AAA...GUGUC______GU__________________AA___________________UCCGA...CNN-3'\n______________AGGCAU__GGAUAUGAAU____CGGC__AAUG_GAUGG__GAAAAUG\n______________\|\|\|\|\|\|__:\|\|:\|\|\|\|\|:____\|\|\|\|__\|\|\|\|_\|\|\|\|:__\|\|\|\|\|::\n______________UCCGUA__UCUGUACUUG____GCCG__UUAC_CUACU__CUUUUGU\n3'-GUU...CCUAC__________________AUGU____G_____U_____UA_______UUAUU...GGA-5'\n_____________\|_______________________________________________\|\n_____________199_____________________________________________155\n	271 -- 277	155 -- 161	-35.3246 kcal/mol	8.28493 kcal/mol	13.976 kcal/mol	237 277@LB@ \| \|@LB@5'-AAA...GUGUC GU AA UCCGA...CNN-3'@LB@ AGGCAU GGAUAUGAAU CGGC AAUG GAUGG GAAAAUG@LB@ \|\|\|\|\|\| :\|\|:\|\|\|\|\|: \|\|\|\| \|\|\|\| \|\|\|\|: \|\|\|\|\|::@LB@ UCCGUA UCUGUACUUG GCCG UUAC CUACU CUUUUGU@LB@3'-GUU...CCUAC AUGU G U UA UUAUU...GGA-5'@LB@ \| \|@LB@ 199 155@LB@@LB@@LB@Energy -20.88 kcal/mol@LB@Hybridization Energy -35.3246 kcal/mol@LB@Unfolding Energy - mRNA 8.28493 kcal/mol@LB@Unfolding Energy - sRNA 13.976 kcal/mol@LB@@LB@Position - mRNA 238 -- 276@LB@Position - sRNA 156 -- 198@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 155 -- 161@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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