Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
61	0.01458	0.6412	lmo2589	N/A	-18.59	0.019740	180 -- 258	153 -- 210	TetR family transcriptional regulator	_____________179__________259\n_____________\|____________\|\n5'-ACU...AACAG____________UGUUU...CUG-3'\n______________AAC_GGGUGGGG\n______________\|\|\|_\|\|:\|\|:\|\|\n______________UUG_CCUACUCC\n3'-GUU...ACGUA___C________GUAUC...GGA-5'\n_____________\|____________\|\n_____________211__________152\n	186 -- 192	197 -- 203	-13.3669 kcal/mol	1.19489 kcal/mol	3.58567 kcal/mol	179 259@LB@ \| \|@LB@5'-ACU...AACAG UGUUU...CUG-3'@LB@ AAC GGGUGGGG@LB@ \|\|\| \|\|:\|\|:\|\|@LB@ UUG CCUACUCC@LB@3'-GUU...ACGUA C GUAUC...GGA-5'@LB@ \| \|@LB@ 211 152@LB@@LB@@LB@Energy -18.59 kcal/mol@LB@Hybridization Energy -13.3669 kcal/mol@LB@Unfolding Energy - mRNA 1.19489 kcal/mol@LB@Unfolding Energy - sRNA 3.58567 kcal/mol@LB@@LB@Position - mRNA 180 -- 258@LB@Position - sRNA 153 -- 210@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 197 -- 203@LB@
63	0.01551	0.6624	lmo2613	rplO	-17.63	0.030853	185 -- 213	177 -- 203	50S ribosomal protein L15	_____________184______214\n_____________\|________\|\n5'-AAU...AUUCG________UCACU...GCG-3'\n______________UGGGAUGA\n______________:\|\|\|\|\|\|\|\n______________GCCCUACU\n3'-GUU...GUAUU________CCGUA...GGA-5'\n_____________\|________\|\n_____________204______176\n	98 -- 104	198 -- 204	-11.9514 kcal/mol	5.35707 kcal/mol	0.374397 kcal/mol	184 214@LB@ \| \|@LB@5'-AAU...AUUCG UCACU...GCG-3'@LB@ UGGGAUGA@LB@ :\|\|\|\|\|\|\|@LB@ GCCCUACU@LB@3'-GUU...GUAUU CCGUA...GGA-5'@LB@ \| \|@LB@ 204 176@LB@@LB@@LB@Energy -17.63 kcal/mol@LB@Hybridization Energy -11.9514 kcal/mol@LB@Unfolding Energy - mRNA 5.35707 kcal/mol@LB@Unfolding Energy - sRNA 0.374397 kcal/mol@LB@@LB@Position - mRNA 185 -- 213@LB@Position - sRNA 177 -- 203@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 198 -- 204@LB@
187	0.05427	0.7607	lmo2654	fus	-15.17	0.090605	196 -- 212	168 -- 184	elongation factor G	_____________195____________213\n_____________\|______________\|\n5'-GAG...AUACA__________A___UUCUC...AUA-3'\n______________ACAUGGCUAG_GAG\n______________\|\|\|:\|\|\|:\|:_\|\|\|\n______________UGUGCCGGUU_CUC\n3'-GUU...CUUGA__________A___UACUU...GGA-5'\n_____________\|______________\|\n_____________185____________167\n	202 -- 208	172 -- 178	-17.9516 kcal/mol	0.731619 kcal/mol	6.23346 kcal/mol	195 213@LB@ \| \|@LB@5'-GAG...AUACA A UUCUC...AUA-3'@LB@ ACAUGGCUAG GAG@LB@ \|\|\|:\|\|\|:\|: \|\|\|@LB@ UGUGCCGGUU CUC@LB@3'-GUU...CUUGA A UACUU...GGA-5'@LB@ \| \|@LB@ 185 167@LB@@LB@@LB@Energy -15.17 kcal/mol@LB@Hybridization Energy -17.9516 kcal/mol@LB@Unfolding Energy - mRNA 0.731619 kcal/mol@LB@Unfolding Energy - sRNA 6.23346 kcal/mol@LB@@LB@Position - mRNA 196 -- 212@LB@Position - sRNA 168 -- 184@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 172 -- 178@LB@
137	0.03627	0.6968	lmo2666	N/A	-15.01	0.096662	42 -- 111	153 -- 211	PTS galacticol transporter subunit IIB	_____________41__________________112\n_____________\|___________________\|\n5'-CUC...ACUCA___CGAAA___________AGUAA...UGG-3'\n______________GAG_____GAUGAAGACGA\n______________\|\|\|_____:\|\|\|\|\|:\|\|:\|\n______________CUC_____UUACUUUUGUU\n3'-GUU...GGUUA___UAC_____________UAUUA...GGA-5'\n_____________\|___________________\|\n_____________212_________________152\n	104 -- 110	154 -- 160	-11.8615 kcal/mol	1.60013 kcal/mol	0.933796 kcal/mol	41 112@LB@ \| \|@LB@5'-CUC...ACUCA CGAAA AGUAA...UGG-3'@LB@ GAG GAUGAAGACGA@LB@ \|\|\| :\|\|\|\|\|:\|\|:\|@LB@ CUC UUACUUUUGUU@LB@3'-GUU...GGUUA UAC UAUUA...GGA-5'@LB@ \| \|@LB@ 212 152@LB@@LB@@LB@Energy -15.01 kcal/mol@LB@Hybridization Energy -11.8615 kcal/mol@LB@Unfolding Energy - mRNA 1.60013 kcal/mol@LB@Unfolding Energy - sRNA 0.933796 kcal/mol@LB@@LB@Position - mRNA 42 -- 111@LB@Position - sRNA 153 -- 211@LB@Position Seed - mRNA 104 -- 110@LB@Position Seed - sRNA 154 -- 160@LB@
198	0.05669	0.7607	lmo2670	N/A	-16.54	0.050282	118 -- 190	141 -- 216		_____________117_________________191\n_____________\|___________________\|\n5'-AAU...AUUUG_________UACC______UUCAA...UCU-3'\n______________GAUGAGAUG____GGGAAC\n______________:\|\|\|\|\|\|\|\|____\|::\|\|\|\n______________UUACUCUAC____CUUUUG\n3'-GUU...GCCGG_________UUA_______UUUAU...GGA-5'\n_____________\|___________________\|\n_____________217_________________140\n	72 -- 78	165 -- 171	-16.0384 kcal/mol	2.83906 kcal/mol	0.941471 kcal/mol	117 191@LB@ \| \|@LB@5'-AAU...AUUUG UACC UUCAA...UCU-3'@LB@ GAUGAGAUG GGGAAC@LB@ :\|\|\|\|\|\|\|\| \|::\|\|\|@LB@ UUACUCUAC CUUUUG@LB@3'-GUU...GCCGG UUA UUUAU...GGA-5'@LB@ \| \|@LB@ 217 140@LB@@LB@@LB@Energy -16.54 kcal/mol@LB@Hybridization Energy -16.0384 kcal/mol@LB@Unfolding Energy - mRNA 2.83906 kcal/mol@LB@Unfolding Energy - sRNA 0.941471 kcal/mol@LB@@LB@Position - mRNA 118 -- 190@LB@Position - sRNA 141 -- 216@LB@Position Seed - mRNA 72 -- 78@LB@Position Seed - sRNA 165 -- 171@LB@
34	0.007076	0.552	lmo2678	N/A	-19.30	0.013995	184 -- 246	155 -- 207	XRE family transcriptional regulator	_____________183__________________________________________________________247\n_____________\|____________________________________________________________\|\n5'-GAA...AGACG____AG_____AA______A__AA______U___CUAAUUGUGGA__________U____UCAGU...AAG-3'\n______________GAGG__AUGGA__AUGAAC_GC__GCGGCU_GUG___________AGAUGAA_GA_GGCA\n______________\|\|\|\|__\|\|:\|\|__\|\|\|\|\|\|_:\|__:\|\|\|\|:_:\|\|___________\|\|\|\|\|\|\|_\|\|_::\|\|\n______________CUCC__UAUCU__UACUUG_UG__UGCCGG_UAC___________UCUACUU_CU_UUGU\n3'-GUU...CCCUA____G______G_______A__________U_____________________A__U____UUAUU...GGA-5'\n_____________\|____________________________________________________________\|\n_____________208__________________________________________________________154\n	233 -- 239	163 -- 169	-37.3867 kcal/mol	12.1272 kcal/mol	13.9822 kcal/mol	183 247@LB@ \| \|@LB@5'-GAA...AGACG AG AA A AA U CUAAUUGUGGA U UCAGU...AAG-3'@LB@ GAGG AUGGA AUGAAC GC GCGGCU GUG AGAUGAA GA GGCA@LB@ \|\|\|\| \|\|:\|\| \|\|\|\|\|\| :\| :\|\|\|\|: :\|\| \|\|\|\|\|\|\| \|\| ::\|\|@LB@ CUCC UAUCU UACUUG UG UGCCGG UAC UCUACUU CU UUGU@LB@3'-GUU...CCCUA G G A U A U UUAUU...GGA-5'@LB@ \| \|@LB@ 208 154@LB@@LB@@LB@Energy -19.30 kcal/mol@LB@Hybridization Energy -37.3867 kcal/mol@LB@Unfolding Energy - mRNA 12.1272 kcal/mol@LB@Unfolding Energy - sRNA 13.9822 kcal/mol@LB@@LB@Position - mRNA 184 -- 246@LB@Position - sRNA 155 -- 207@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 163 -- 169@LB@
21	0.003699	0.4469	lmo2681	kdpB	-18.50	0.020601	185 -- 204	154 -- 173	potassium-transporting ATPase subunit B	_____________184__________________205\n_____________\|____________________\|\n5'-GCA...UUUCA____________________UGGAA...AUG-3'\n______________AAUGAGGUGAAUGGAAAUGA\n______________\|\|\|\|\|\|:\|\|\|\|\|\|:\|\|\|::\|\n______________UUACUCUACUUACUUUUGUU\n3'-GUU...GCCGG____________________UAUUA...GGA-5'\n_____________\|____________________\|\n_____________174__________________153\n	197 -- 203	155 -- 161	-22.9313 kcal/mol	3.41 kcal/mol	0.959215 kcal/mol	184 205@LB@ \| \|@LB@5'-GCA...UUUCA UGGAA...AUG-3'@LB@ AAUGAGGUGAAUGGAAAUGA@LB@ \|\|\|\|\|\|:\|\|\|\|\|\|:\|\|\|::\|@LB@ UUACUCUACUUACUUUUGUU@LB@3'-GUU...GCCGG UAUUA...GGA-5'@LB@ \| \|@LB@ 174 153@LB@@LB@@LB@Energy -18.50 kcal/mol@LB@Hybridization Energy -22.9313 kcal/mol@LB@Unfolding Energy - mRNA 3.41 kcal/mol@LB@Unfolding Energy - sRNA 0.959215 kcal/mol@LB@@LB@Position - mRNA 185 -- 204@LB@Position - sRNA 154 -- 173@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 155 -- 161@LB@
134	0.0358	0.6968	lmo2682	kdpA	-15.50	0.078936	69 -- 121	151 -- 202	potassium-transporting ATPase subunit A	_____________68_____________________________________________122\n_____________\|______________________________________________\|\n5'-ACA...UUGGU___AU___UG_____AAGAG______G___________CG______GGGUG...AAA-3'\n______________AGG__UAG__GUGGA_____UACGGU_AA_GGGGUGAA__GAAAAU\n______________\|\|\|__\|\|\|__:\|\|:\|_____:\|\|\|\|:_\|\|_\|:\|:\|\|\|\|__\|\|\|\|\|:\n______________UCC__AUC__UACUU_____GUGCCG_UU_CUCUACUU__CUUUUG\n3'-GUU...CCUAC___GU___UG_____GAU________G__A________A_______UUUAU...GGA-5'\n_____________\|______________________________________________\|\n_____________203____________________________________________150\n	102 -- 108	163 -- 169	-34.0107 kcal/mol	9.08348 kcal/mol	13.9626 kcal/mol	68 122@LB@ \| \|@LB@5'-ACA...UUGGU AU UG AAGAG G CG GGGUG...AAA-3'@LB@ AGG UAG GUGGA UACGGU AA GGGGUGAA GAAAAU@LB@ \|\|\| \|\|\| :\|\|:\| :\|\|\|\|: \|\| \|:\|:\|\|\|\| \|\|\|\|\|:@LB@ UCC AUC UACUU GUGCCG UU CUCUACUU CUUUUG@LB@3'-GUU...CCUAC GU UG GAU G A A UUUAU...GGA-5'@LB@ \| \|@LB@ 203 150@LB@@LB@@LB@Energy -15.50 kcal/mol@LB@Hybridization Energy -34.0107 kcal/mol@LB@Unfolding Energy - mRNA 9.08348 kcal/mol@LB@Unfolding Energy - sRNA 13.9626 kcal/mol@LB@@LB@Position - mRNA 69 -- 121@LB@Position - sRNA 151 -- 202@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 163 -- 169@LB@
46	0.01036	0.599	lmo2695	N/A	-19.87	0.010617	150 -- 211	156 -- 209	dihydroxyacetone kinase subunit DhaK	_____________149_________________________________________________________________212\n_____________\|___________________________________________________________________\|\n5'-UUA...UUCUA____A____AAU_____________________UAUAAAGGA____UUU__UC______________UCUUA...CUC-3'\n______________GUAA_GGGA___UGAGGUA_AGA___GGACUAU_________GGUU___UG__AGAUGAA_GAAGAU\n______________:\|\|\|_\|\|\|\|___\|\|\|\|\|:\|_\|\|\|___\|:\|\|\|\|:_________\|\|::___\|\|__\|\|\|\|\|\|\|_\|\|\|:\|:\n______________UAUU_CCCU___ACUCCGU_UCU___CUUGAUG_________CCGG___AC__UCUACUU_CUUUUG\n3'-GUU...AUACG____G______________A___GUA_______UG___________UU____________A______UUUAU...GGA-5'\n_____________\|___________________________________________________________________\|\n_____________210_________________________________________________________________155\n	199 -- 205	163 -- 169	-36.4151 kcal/mol	9.16424 kcal/mol	18.7057 kcal/mol	149 212@LB@ \| \|@LB@5'-UUA...UUCUA A AAU UAUAAAGGA UUU UC UCUUA...CUC-3'@LB@ GUAA GGGA UGAGGUA AGA GGACUAU GGUU UG AGAUGAA GAAGAU@LB@ :\|\|\| \|\|\|\| \|\|\|\|\|:\| \|\|\| \|:\|\|\|\|: \|\|:: \|\| \|\|\|\|\|\|\| \|\|\|:\|:@LB@ UAUU CCCU ACUCCGU UCU CUUGAUG CCGG AC UCUACUU CUUUUG@LB@3'-GUU...AUACG G A GUA UG UU A UUUAU...GGA-5'@LB@ \| \|@LB@ 210 155@LB@@LB@@LB@Energy -19.87 kcal/mol@LB@Hybridization Energy -36.4151 kcal/mol@LB@Unfolding Energy - mRNA 9.16424 kcal/mol@LB@Unfolding Energy - sRNA 18.7057 kcal/mol@LB@@LB@Position - mRNA 150 -- 211@LB@Position - sRNA 156 -- 209@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 163 -- 169@LB@
143	0.03726	0.6968	lmo2701	N/A	-17.97	0.026314	78 -- 126	156 -- 198		_____________77_________________________________________________127\n_____________\|__________________________________________________\|\n5'-GAA...CCUUC_______AAAGUA___A____AGC________UUCCA_____________UUAGA...UCA-3'\n______________AGGCAUA______ACG_GAAU___GCAUGGCC______GUGGGUGGAGAC\n______________\|\|\|\|\|\|\|______\|\|:_\|\|\|:___:\|\|:\|\|\|\|______:\|\|::\|\|:\|:\|\|\n______________UCCGUAU______UGU_CUUG___UGUGCCGG______UACUUACUUUUG\n3'-GUU...CCUAC_______C________A____A__________UUACUC____________UUUAU...GGA-5'\n_____________\|__________________________________________________\|\n_____________199________________________________________________155\n	120 -- 126	156 -- 162	-37.6493 kcal/mol	12.7788 kcal/mol	13.9626 kcal/mol	77 127@LB@ \| \|@LB@5'-GAA...CCUUC AAAGUA A AGC UUCCA UUAGA...UCA-3'@LB@ AGGCAUA ACG GAAU GCAUGGCC GUGGGUGGAGAC@LB@ \|\|\|\|\|\|\| \|\|: \|\|\|: :\|\|:\|\|\|\| :\|\|::\|\|:\|:\|\|@LB@ UCCGUAU UGU CUUG UGUGCCGG UACUUACUUUUG@LB@3'-GUU...CCUAC C A A UUACUC UUUAU...GGA-5'@LB@ \| \|@LB@ 199 155@LB@@LB@@LB@Energy -17.97 kcal/mol@LB@Hybridization Energy -37.6493 kcal/mol@LB@Unfolding Energy - mRNA 12.7788 kcal/mol@LB@Unfolding Energy - sRNA 13.9626 kcal/mol@LB@@LB@Position - mRNA 78 -- 126@LB@Position - sRNA 156 -- 198@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 156 -- 162@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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