Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
149	0.0387	0.6968	lmo1809	plsX	-16.53	0.050633	188 -- 233	156 -- 203	glycerol-3-phosphate acyltransferase PlsX	_____________187________________234\n_____________\|__________________\|\n5'-ACA...CUGUA____C_____________CACGC...UUC-3'\n______________GAUG_GAUGGGUGGAGAU\n______________:\|\|\|_\|\|\|\|::\|\|:\|:\|:\n______________UUAC_CUACUUACUUUUG\n3'-GUU...GCCGG____U_____________UUUAU...GGA-5'\n_____________\|__________________\|\n_____________204________________155\n	227 -- 233	156 -- 162	-17.4325 kcal/mol	7.3364 kcal/mol	0.941471 kcal/mol	187 234@LB@ \| \|@LB@5'-ACA...CUGUA C CACGC...UUC-3'@LB@ GAUG GAUGGGUGGAGAU@LB@ :\|\|\| \|\|\|\|::\|\|:\|:\|:@LB@ UUAC CUACUUACUUUUG@LB@3'-GUU...GCCGG U UUUAU...GGA-5'@LB@ \| \|@LB@ 204 155@LB@@LB@@LB@Energy -16.53 kcal/mol@LB@Hybridization Energy -17.4325 kcal/mol@LB@Unfolding Energy - mRNA 7.3364 kcal/mol@LB@Unfolding Energy - sRNA 0.941471 kcal/mol@LB@@LB@Position - mRNA 188 -- 233@LB@Position - sRNA 156 -- 203@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 156 -- 162@LB@
123	0.03258	0.6968	lmo1821	N/A	-14.83	0.104144	189 -- 278	153 -- 220	phosphoprotein phosphatase	_____________188_________279\n_____________\|___________\|\n5'-AGA...AUCAU___________CGGCG...UUG-3'\n______________AAUGAAGACAA\n______________\|\|\|\|\|\|:\|\|\|\|\n______________UUACUUUUGUU\n3'-GUU...UCUAC___________UAUUA...GGA-5'\n_____________\|___________\|\n_____________221_________152\n	250 -- 256	154 -- 160	-9.76744 kcal/mol	1.01794 kcal/mol	0.610258 kcal/mol	188 279@LB@ \| \|@LB@5'-AGA...AUCAU CGGCG...UUG-3'@LB@ AAUGAAGACAA@LB@ \|\|\|\|\|\|:\|\|\|\|@LB@ UUACUUUUGUU@LB@3'-GUU...UCUAC UAUUA...GGA-5'@LB@ \| \|@LB@ 221 152@LB@@LB@@LB@Energy -14.83 kcal/mol@LB@Hybridization Energy -9.76744 kcal/mol@LB@Unfolding Energy - mRNA 1.01794 kcal/mol@LB@Unfolding Energy - sRNA 0.610258 kcal/mol@LB@@LB@Position - mRNA 189 -- 278@LB@Position - sRNA 153 -- 220@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 154 -- 160@LB@
31	0.00657	0.5515	lmo1828	N/A	-18.66	0.019040	211 -- 279	154 -- 211		_____________210__________280\n_____________\|____________\|\n5'-AGU...GAAAA____________UUGGG...AAU-3'\n______________GCAUGACGGGAU\n______________\|\|\|\|:\|\|\|\|\|\|\|\n______________CGUAUUGCCCUA\n3'-GUU...GGAUA____________CUCCG...GGA-5'\n_____________\|____________\|\n_____________212__________153\n	216 -- 222	200 -- 206	-19.8213 kcal/mol	2.93239 kcal/mol	6.43166 kcal/mol	210 280@LB@ \| \|@LB@5'-AGU...GAAAA UUGGG...AAU-3'@LB@ GCAUGACGGGAU@LB@ \|\|\|\|:\|\|\|\|\|\|\|@LB@ CGUAUUGCCCUA@LB@3'-GUU...GGAUA CUCCG...GGA-5'@LB@ \| \|@LB@ 212 153@LB@@LB@@LB@Energy -18.66 kcal/mol@LB@Hybridization Energy -19.8213 kcal/mol@LB@Unfolding Energy - mRNA 2.93239 kcal/mol@LB@Unfolding Energy - sRNA 6.43166 kcal/mol@LB@@LB@Position - mRNA 211 -- 279@LB@Position - sRNA 154 -- 211@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 200 -- 206@LB@
5	0.001245	0.4469	lmo1835	carB	-22.95	0.002153	176 -- 228	154 -- 207	carbamoyl-phosphate synthetase	_____________175____________________________________________________________229\n_____________\|______________________________________________________________\|\n5'-AAA...UGGAA___AUG_______AA_______________________C____AC_C__C__UAUA______AUUCU...CUU-3'\n______________AUG___AAUGGGA__GAGG___AGG_GUGAACUACAUG_CUAA__G_GA_GA____AAAACA\n______________\|\|\|___\|\|:\|\|\|\|__\|\|\|\|___\|\|:_:\|\|\|\|\|\|\|\|\|:\|_\|:\|\|__\|_\|\|_\|\|____\|\|\|\|\|\|\n______________UAC___UUGCCCU__CUCC___UCU_UACUUGAUGUGC_GGUU__C_CU_CU____UUUUGU\n3'-GUU...GCGGA___GUA_______A_____GUA___G____________C____A__U__A__UAC_______UUAUU...GGA-5'\n_____________\|______________________________________________________________\|\n_____________208____________________________________________________________153\n	194 -- 200	180 -- 186	-39.6119 kcal/mol	8.25276 kcal/mol	19.5968 kcal/mol	175 229@LB@ \| \|@LB@5'-AAA...UGGAA AUG AA C AC C C UAUA AUUCU...CUU-3'@LB@ AUG AAUGGGA GAGG AGG GUGAACUACAUG CUAA G GA GA AAAACA@LB@ \|\|\| \|\|:\|\|\|\| \|\|\|\| \|\|: :\|\|\|\|\|\|\|\|\|:\| \|:\|\| \| \|\| \|\| \|\|\|\|\|\|@LB@ UAC UUGCCCU CUCC UCU UACUUGAUGUGC GGUU C CU CU UUUUGU@LB@3'-GUU...GCGGA GUA A GUA G C A U A UAC UUAUU...GGA-5'@LB@ \| \|@LB@ 208 153@LB@@LB@@LB@Energy -22.95 kcal/mol@LB@Hybridization Energy -39.6119 kcal/mol@LB@Unfolding Energy - mRNA 8.25276 kcal/mol@LB@Unfolding Energy - sRNA 19.5968 kcal/mol@LB@@LB@Position - mRNA 176 -- 228@LB@Position - sRNA 154 -- 207@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 180 -- 186@LB@
168	0.04649	0.7403	lmo1875	N/A	-18.37	0.021889	174 -- 204	150 -- 181	ABC transporter ATP-binding protein	_____________173_______________205\n_____________\|_________________\|\n5'-ACA...AACAG__A______________AUGAA...GUU-3'\n______________GA_AUGGAUGGGAAUAA\n______________\|\|_\|\|\|:\|\|\|::\|\|:\|\|\n______________CU_UACUUACUUUUGUU\n3'-GUU...GGUUA__C______________UAUUA...GGA-5'\n_____________\|_________________\|\n_____________182_______________149\n	193 -- 199	155 -- 161	-14.111 kcal/mol	2.1341 kcal/mol	0.933796 kcal/mol	173 205@LB@ \| \|@LB@5'-ACA...AACAG A AUGAA...GUU-3'@LB@ GA AUGGAUGGGAAUAA@LB@ \|\| \|\|\|:\|\|\|::\|\|:\|\|@LB@ CU UACUUACUUUUGUU@LB@3'-GUU...GGUUA C UAUUA...GGA-5'@LB@ \| \|@LB@ 182 149@LB@@LB@@LB@Energy -18.37 kcal/mol@LB@Hybridization Energy -14.111 kcal/mol@LB@Unfolding Energy - mRNA 2.1341 kcal/mol@LB@Unfolding Energy - sRNA 0.933796 kcal/mol@LB@@LB@Position - mRNA 174 -- 204@LB@Position - sRNA 150 -- 181@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 155 -- 161@LB@
1	1.731e-05	0.04657	lmo1883	N/A	-26.54	0.000269	199 -- 265	114 -- 169	chitinase	_____________198_________________________________________________________________266\n_____________\|___________________________________________________________________\|\n5'-GCG...AAAGU________G_________________GG___UU_____UU__UUUUAGUU____CGG______GG__AUUUG...ACG-3'\n______________AGAUGAAU_GAAAACAAGUAAUGGUU__UGG__UGUCU__AC________GGUG___GACUGG__GC\n______________\|\|\|\|\|\|\|\|_\|\|\|\|\|\|\|\|:\|\|\|\|\|:\|\|__\|\|\|__\|\|\|\|\|__\|\|________\|:\|\|___\|\|\|\|:\|__\|\|\n______________UCUACUUA_CUUUUGUUUAUUACUAA__ACC__ACAGA__UG________CUAC___CUGAUC__CG\n3'-GUU...GUUAC__________________________A___________UU__U___________AGA______AA__GUAAG...GGA-5'\n_____________\|___________________________________________________________________\|\n_____________170_________________________________________________________________113\n	210 -- 216	153 -- 159	-51.3221 kcal/mol	15.9506 kcal/mol	18.5045 kcal/mol	198 266@LB@ \| \|@LB@5'-GCG...AAAGU G GG UU UU UUUUAGUU CGG GG AUUUG...ACG-3'@LB@ AGAUGAAU GAAAACAAGUAAUGGUU UGG UGUCU AC GGUG GACUGG GC@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|\|:\|\|\|\|\|:\|\| \|\|\| \|\|\|\|\| \|\| \|:\|\| \|\|\|\|:\| \|\|@LB@ UCUACUUA CUUUUGUUUAUUACUAA ACC ACAGA UG CUAC CUGAUC CG@LB@3'-GUU...GUUAC A UU U AGA AA GUAAG...GGA-5'@LB@ \| \|@LB@ 170 113@LB@@LB@@LB@Energy -26.54 kcal/mol@LB@Hybridization Energy -51.3221 kcal/mol@LB@Unfolding Energy - mRNA 15.9506 kcal/mol@LB@Unfolding Energy - sRNA 18.5045 kcal/mol@LB@@LB@Position - mRNA 199 -- 265@LB@Position - sRNA 114 -- 169@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 153 -- 159@LB@
177	0.05015	0.7607	lmo1905	cca	-16.43	0.052746	154 -- 208	160 -- 211	tRNA CCA-pyrophosphorylase	_____________153_______________209\n_____________\|_________________\|\n5'-UUA...AGGAG______CA_________CGUUU...UGC-3'\n______________CGAUGA__GAUGAAUGA\n______________\|:\|\|\|\|__\|\|\|\|\|\|\|\|\|\n______________GUUACU__CUACUUACU\n3'-GUU...UGCCG_________________UUUGU...GGA-5'\n_____________\|_________________\|\n_____________212_______________159\n	202 -- 208	160 -- 166	-15.3935 kcal/mol	2.32419 kcal/mol	0.846187 kcal/mol	153 209@LB@ \| \|@LB@5'-UUA...AGGAG CA CGUUU...UGC-3'@LB@ CGAUGA GAUGAAUGA@LB@ \|:\|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ GUUACU CUACUUACU@LB@3'-GUU...UGCCG UUUGU...GGA-5'@LB@ \| \|@LB@ 212 159@LB@@LB@@LB@Energy -16.43 kcal/mol@LB@Hybridization Energy -15.3935 kcal/mol@LB@Unfolding Energy - mRNA 2.32419 kcal/mol@LB@Unfolding Energy - sRNA 0.846187 kcal/mol@LB@@LB@Position - mRNA 154 -- 208@LB@Position - sRNA 160 -- 211@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 160 -- 166@LB@
33	0.006763	0.5515	lmo1941	N/A	-21.42	0.004845	223 -- 286	154 -- 207		_____________222___________________________________________________________________287\n_____________\|_____________________________________________________________________\|\n5'-UAG...AGAAA_______AUACGAAGAACC___UGAUUAU________UU___AA_________________UC______UGAAC...UGU-3'\n______________AACGGGA____________GGC_______GACAUGAG__CUG___GGCCA___AGAUGAAU___AACAA\n______________\|\|\|\|\|\|\|____________\|\|\|_______\|\|\|\|\|\|\|:__\|\|:___\|\|\|\|\|___\|\|\|\|\|\|\|\|___\|\|\|\|\|\n______________UUGCCCU____________CCG_______CUGUACUU__GAU___CCGGU___UCUACUUA___UUGUU\n3'-GUU...ACGUA_______ACU____________UAU_________________GUG_____UAC________CUU_____UAUUA...GGA-5'\n_____________\|_____________________________________________________________________\|\n_____________208___________________________________________________________________153\n	273 -- 279	162 -- 168	-39.5283 kcal/mol	10.6608 kcal/mol	18.6655 kcal/mol	222 287@LB@ \| \|@LB@5'-UAG...AGAAA AUACGAAGAACC UGAUUAU UU AA UC UGAAC...UGU-3'@LB@ AACGGGA GGC GACAUGAG CUG GGCCA AGAUGAAU AACAA@LB@ \|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|: \|\|: \|\|\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|@LB@ UUGCCCU CCG CUGUACUU GAU CCGGU UCUACUUA UUGUU@LB@3'-GUU...ACGUA ACU UAU GUG UAC CUU UAUUA...GGA-5'@LB@ \| \|@LB@ 208 153@LB@@LB@@LB@Energy -21.42 kcal/mol@LB@Hybridization Energy -39.5283 kcal/mol@LB@Unfolding Energy - mRNA 10.6608 kcal/mol@LB@Unfolding Energy - sRNA 18.6655 kcal/mol@LB@@LB@Position - mRNA 223 -- 286@LB@Position - sRNA 154 -- 207@LB@Position Seed - mRNA 273 -- 279@LB@Position Seed - sRNA 162 -- 168@LB@
92	0.02404	0.6733	lmo1947	resE	-16.63	0.048416	181 -- 212	141 -- 170	two-component sensor histidine kinas	_____________180_________________213\n_____________\|___________________\|\n5'-AAG...ACCGA_______AUA_________UUGGA...UUU-3'\n______________GGUGAGA___GAUGAAGAU\n______________::\|\|\|\|\|___:\|\|\|\|\|:\|:\n______________UUACUCU___UUACUUUUG\n3'-GUU...GCCGG_______AC__________UUUAU...GGA-5'\n_____________\|___________________\|\n_____________171_________________140\n	202 -- 208	156 -- 162	-13.4823 kcal/mol	2.25638 kcal/mol	0.941471 kcal/mol	180 213@LB@ \| \|@LB@5'-AAG...ACCGA AUA UUGGA...UUU-3'@LB@ GGUGAGA GAUGAAGAU@LB@ ::\|\|\|\|\| :\|\|\|\|\|:\|:@LB@ UUACUCU UUACUUUUG@LB@3'-GUU...GCCGG AC UUUAU...GGA-5'@LB@ \| \|@LB@ 171 140@LB@@LB@@LB@Energy -16.63 kcal/mol@LB@Hybridization Energy -13.4823 kcal/mol@LB@Unfolding Energy - mRNA 2.25638 kcal/mol@LB@Unfolding Energy - sRNA 0.941471 kcal/mol@LB@@LB@Position - mRNA 181 -- 212@LB@Position - sRNA 141 -- 170@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 156 -- 162@LB@
12	0.002933	0.4469	lmo1952	lysA	-19.68	0.011630	182 -- 245	154 -- 213	diaminopimelate decarboxylase	_____________181___________________________________________________________246\n_____________\|_____________________________________________________________\|\n5'-UUG...CUAUA_____AAGG______U__________________A______UG___ACA_______U____GCAGA...CGC-3'\n______________AUGUA____UGGGAU__GGCGU_GACGU___UUG_ACGGCU___GA___AUGAAUG_AAAU\n______________\|\|\|:\|____:\|\|\|\|\|__\|\|\|:\|_\|\|\|:\|___:\|:_\|\|\|\|\|:___\|\|___\|\|\|\|\|\|\|_\|\|\|:\n______________UACGU____GCCCUA__CCGUA_CUGUA___GAU_UGCCGG___CU___UACUUAC_UUUG\n3'-GUU...GCGGA_____AUU_______CU_____U_____CUU___G______UUA__C_________U____UUUAU...GGA-5'\n_____________\|_____________________________________________________________\|\n_____________214___________________________________________________________153\n	225 -- 231	161 -- 167	-39.877 kcal/mol	10.395 kcal/mol	19.5834 kcal/mol	181 246@LB@ \| \|@LB@5'-UUG...CUAUA AAGG U A UG ACA U GCAGA...CGC-3'@LB@ AUGUA UGGGAU GGCGU GACGU UUG ACGGCU GA AUGAAUG AAAU@LB@ \|\|\|:\| :\|\|\|\|\| \|\|\|:\| \|\|\|:\| :\|: \|\|\|\|\|: \|\| \|\|\|\|\|\|\| \|\|\|:@LB@ UACGU GCCCUA CCGUA CUGUA GAU UGCCGG CU UACUUAC UUUG@LB@3'-GUU...GCGGA AUU CU U CUU G UUA C U UUUAU...GGA-5'@LB@ \| \|@LB@ 214 153@LB@@LB@@LB@Energy -19.68 kcal/mol@LB@Hybridization Energy -39.877 kcal/mol@LB@Unfolding Energy - mRNA 10.395 kcal/mol@LB@Unfolding Energy - sRNA 19.5834 kcal/mol@LB@@LB@Position - mRNA 182 -- 245@LB@Position - sRNA 154 -- 213@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 161 -- 167@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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