Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
30	0.001906	0.2094	b0616	citE	-12.56	0.043465	103 -- 116	8 -- 21	citrate lyase citryl-ACP lyase (beta) subunit	_____________102____________117\n_____________\|______________\|\n5'-CAA...AUGUC_________G____ACUGG...UCU-3'\n______________GAUGACAAA_GCGC\n______________\|\|\|:\|\|\|\|\|_\|\|\|\|\n______________CUAUUGUUU_CGCG\n3'-UUU...UACUA_________A____CAGAAAG-5'\n_____________\|______________\|\n_____________22_____________7\n	105 -- 111	13 -- 19	-17.9482 kcal/mol	4.14919 kcal/mol	1.23638 kcal/mol	102 117@LB@ \| \|@LB@5'-CAA...AUGUC G ACUGG...UCU-3'@LB@ GAUGACAAA GCGC@LB@ \|\|\|:\|\|\|\|\| \|\|\|\|@LB@ CUAUUGUUU CGCG@LB@3'-UUU...UACUA A CAGAAAG-5'@LB@ \| \|@LB@ 22 7@LB@@LB@@LB@Energy -12.56 kcal/mol@LB@Hybridization Energy -17.9482 kcal/mol@LB@Unfolding Energy - mRNA 4.14919 kcal/mol@LB@Unfolding Energy - sRNA 1.23638 kcal/mol@LB@@LB@Position - mRNA 103 -- 116@LB@Position - sRNA 8 -- 21@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 13 -- 19@LB@
128	0.01258	0.3104	b0677	nagA	-7.18	0.336330	272 -- 297	1 -- 22	N-acetylglucosamine-6-phosphate deacetylase	_____________271________________________298\n_____________\|__________________________\|\n5'-AAC...AUCGC_______CU___UAAAA______G__CGG-3'\n______________UGAUGGC__GAU_____GCGUCU_UC\n______________\|\|\|\|::\|__:\|\|_____\|\|\|\|\|\|_\|\|\n______________ACUAUUG__UUA_____CGCAGA_AG\n3'-UUU...CUACU_______U____CG_________A__-5'\n_____________\|_________________________\|\n_____________23________________________1\n	272 -- 278	16 -- 22	-15.3811 kcal/mol	6.93964 kcal/mol	1.25963 kcal/mol	271 298@LB@ \| \|@LB@5'-AAC...AUCGC CU UAAAA G CGG-3'@LB@ UGAUGGC GAU GCGUCU UC@LB@ \|\|\|\|::\| :\|\| \|\|\|\|\|\| \|\|@LB@ ACUAUUG UUA CGCAGA AG@LB@3'-UUU...CUACU U CG A -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -7.18 kcal/mol@LB@Hybridization Energy -15.3811 kcal/mol@LB@Unfolding Energy - mRNA 6.93964 kcal/mol@LB@Unfolding Energy - sRNA 1.25963 kcal/mol@LB@@LB@Position - mRNA 272 -- 297@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 272 -- 278@LB@Position Seed - sRNA 16 -- 22@LB@
172	0.01719	0.3104	b0678	nagB	-13.42	0.030481	5 -- 26	1 -- 17	glucosamine-6-phosphate deaminase	_____________4______________________27\n_____________\|______________________\|\n_______5'-UAUC______UAAAC___________GUGAG...GUC-3'\n______________GCAAAU_____GCGUGUCUUUU\n______________:\|\|\|\|\|_____\|\|\|:\|\|\|\|\|\|:\n______________UGUUUA_____CGCGCAGAAAG\n3'-UUU...ACUAU______________________-5'\n_____________\|_____________________\|\n_____________18____________________1\n	20 -- 26	1 -- 7	-18.2092 kcal/mol	3.54695 kcal/mol	1.23994 kcal/mol	4 27@LB@ \| \|@LB@ 5'-UAUC UAAAC GUGAG...GUC-3'@LB@ GCAAAU GCGUGUCUUUU@LB@ :\|\|\|\|\| \|\|\|:\|\|\|\|\|\|:@LB@ UGUUUA CGCGCAGAAAG@LB@3'-UUU...ACUAU -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -13.42 kcal/mol@LB@Hybridization Energy -18.2092 kcal/mol@LB@Unfolding Energy - mRNA 3.54695 kcal/mol@LB@Unfolding Energy - sRNA 1.23994 kcal/mol@LB@@LB@Position - mRNA 5 -- 26@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 20 -- 26@LB@Position Seed - sRNA 1 -- 7@LB@
136	0.01311	0.3104	b0694	kdpE	-14.36	0.020608	275 -- 287	2 -- 14	response regulator in two-component regulatory system with KdpD	_____________274___________288\n_____________\|_____________\|\n5'-ACG...GACGG_____________GAGGC...CGC-3'\n______________GAUGCGCGUCUUU\n______________:\|\|\|\|\|\|\|\|\|\|\|\|\n______________UUACGCGCAGAAA\n3'-UUU...AUUGU_____________G-5'\n_____________\|_____________\|\n_____________15____________1\n	281 -- 287	2 -- 8	-20.3279 kcal/mol	4.79291 kcal/mol	1.17514 kcal/mol	274 288@LB@ \| \|@LB@5'-ACG...GACGG GAGGC...CGC-3'@LB@ GAUGCGCGUCUUU@LB@ :\|\|\|\|\|\|\|\|\|\|\|\|@LB@ UUACGCGCAGAAA@LB@3'-UUU...AUUGU G-5'@LB@ \| \|@LB@ 15 1@LB@@LB@@LB@Energy -14.36 kcal/mol@LB@Hybridization Energy -20.3279 kcal/mol@LB@Unfolding Energy - mRNA 4.79291 kcal/mol@LB@Unfolding Energy - sRNA 1.17514 kcal/mol@LB@@LB@Position - mRNA 275 -- 287@LB@Position - sRNA 2 -- 14@LB@Position Seed - mRNA 281 -- 287@LB@Position Seed - sRNA 2 -- 8@LB@
145	0.01404	0.3104	b0721	sdhC	-10.79	0.088833	202 -- 215	9 -- 22	succinate dehydrogenase membrane subunit binds cytochrome b556	_____________201____________216\n_____________\|______________\|\n5'-UCC...AUUCA______G_______AAAAA...CCG-3'\n______________UGAUAA_AAAUGUG\n______________\|\|\|\|\|\|_\|\|\|\|\|:\|\n______________ACUAUU_UUUACGC\n3'-UUU...CUACU______G_______GCAGAAAG-5'\n_____________\|______________\|\n_____________23_____________8\n	209 -- 215	9 -- 15	-14.1232 kcal/mol	2.09271 kcal/mol	1.23827 kcal/mol	201 216@LB@ \| \|@LB@5'-UCC...AUUCA G AAAAA...CCG-3'@LB@ UGAUAA AAAUGUG@LB@ \|\|\|\|\|\| \|\|\|\|\|:\|@LB@ ACUAUU UUUACGC@LB@3'-UUU...CUACU G GCAGAAAG-5'@LB@ \| \|@LB@ 23 8@LB@@LB@@LB@Energy -10.79 kcal/mol@LB@Hybridization Energy -14.1232 kcal/mol@LB@Unfolding Energy - mRNA 2.09271 kcal/mol@LB@Unfolding Energy - sRNA 1.23827 kcal/mol@LB@@LB@Position - mRNA 202 -- 215@LB@Position - sRNA 9 -- 22@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 9 -- 15@LB@
107	0.01107	0.3104	b0723	sdhA	-7.08	0.348281	169 -- 200	7 -- 42	succinate dehydrogenase flavoprotein subunit	_____________168__________________________________201\n_____________\|____________________________________\|\n5'-GGU...ACGUG____A________UU_______UG____________AUGAA...GUG-3'\n______________AUUU_UGGAUUCG___GUGGUG_____GGGUGUGUG\n______________\|\|:\|_\|\|:\|\|\|\|:___:\|\|:\|\|_____:::\|\|:\|:\|\n______________UAGA_ACUUAAGU___UACUAC_____UUUACGCGC\n3'-UUU...UAAAG____G________CCC______UAUUG_________AGAAAG-5'\n_____________\|____________________________________\|\n_____________43___________________________________6\n	194 -- 200	7 -- 13	-22.2639 kcal/mol	5.75915 kcal/mol	9.42962 kcal/mol	168 201@LB@ \| \|@LB@5'-GGU...ACGUG A UU UG AUGAA...GUG-3'@LB@ AUUU UGGAUUCG GUGGUG GGGUGUGUG@LB@ \|\|:\| \|\|:\|\|\|\|: :\|\|:\|\| :::\|\|:\|:\|@LB@ UAGA ACUUAAGU UACUAC UUUACGCGC@LB@3'-UUU...UAAAG G CCC UAUUG AGAAAG-5'@LB@ \| \|@LB@ 43 6@LB@@LB@@LB@Energy -7.08 kcal/mol@LB@Hybridization Energy -22.2639 kcal/mol@LB@Unfolding Energy - mRNA 5.75915 kcal/mol@LB@Unfolding Energy - sRNA 9.42962 kcal/mol@LB@@LB@Position - mRNA 169 -- 200@LB@Position - sRNA 7 -- 42@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 7 -- 13@LB@
142	0.01346	0.3104	b0729	sucD	-11.71	0.061414	39 -- 57	3 -- 21	succinyl-CoA synthetase NAD(P)-binding alpha subunit	_____________38__________________58\n_____________\|___________________\|\n5'-GUU...GGCGC_________A___G__G__AACGU...CUA-3'\n______________GGUAGCAGA_GUG_GU_UU\n______________\|:\|\|:\|\|:\|_\|:\|_\|\|_\|\|\n______________CUAUUGUUU_CGC_CA_AA\n3'-UUU...UACUA_________A___G__G__AG-5'\n_____________\|___________________\|\n_____________22__________________2\n	41 -- 47	13 -- 19	-17.4316 kcal/mol	4.46052 kcal/mol	1.257 kcal/mol	38 58@LB@ \| \|@LB@5'-GUU...GGCGC A G G AACGU...CUA-3'@LB@ GGUAGCAGA GUG GU UU@LB@ \|:\|\|:\|\|:\| \|:\| \|\| \|\|@LB@ CUAUUGUUU CGC CA AA@LB@3'-UUU...UACUA A G G AG-5'@LB@ \| \|@LB@ 22 2@LB@@LB@@LB@Energy -11.71 kcal/mol@LB@Hybridization Energy -17.4316 kcal/mol@LB@Unfolding Energy - mRNA 4.46052 kcal/mol@LB@Unfolding Energy - sRNA 1.257 kcal/mol@LB@@LB@Position - mRNA 39 -- 57@LB@Position - sRNA 3 -- 21@LB@Position Seed - mRNA 41 -- 47@LB@Position Seed - sRNA 13 -- 19@LB@
81	0.007385	0.3087	b0736	ybgC	-11.71	0.061637	220 -- 237	4 -- 21	acyl-CoA thioester hydrolase	_____________219________________238\n_____________\|__________________\|\n5'-UUG...GUUUC______C___U_______ACUAU...AAA-3'\n______________GAUGGC_GGU_CGCGUCU\n______________\|\|\|::\|_::\|_\|\|\|\|\|\|\|\n______________CUAUUG_UUA_GCGCAGA\n3'-UUU...UACUA______U___C_______AAG-5'\n_____________\|__________________\|\n_____________22_________________3\n	231 -- 237	4 -- 10	-19.027 kcal/mol	6.06673 kcal/mol	1.2552 kcal/mol	219 238@LB@ \| \|@LB@5'-UUG...GUUUC C U ACUAU...AAA-3'@LB@ GAUGGC GGU CGCGUCU@LB@ \|\|\|::\| ::\| \|\|\|\|\|\|\|@LB@ CUAUUG UUA GCGCAGA@LB@3'-UUU...UACUA U C AAG-5'@LB@ \| \|@LB@ 22 3@LB@@LB@@LB@Energy -11.71 kcal/mol@LB@Hybridization Energy -19.027 kcal/mol@LB@Unfolding Energy - mRNA 6.06673 kcal/mol@LB@Unfolding Energy - sRNA 1.2552 kcal/mol@LB@@LB@Position - mRNA 220 -- 237@LB@Position - sRNA 4 -- 21@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 4 -- 10@LB@
5	7.362e-05	0.04801	b0741	pal	-13.90	0.024968	103 -- 115	3 -- 16	peptidoglycan-associated outer membrane lipoprotein	_____________102____________116\n_____________\|______________\|\n5'-GGA...CGUUU______________CCGGC...GCG-3'\n______________CAAA_GCGCGUCUU\n______________\|\|\|\|_\|\|\|\|\|\|\|\|\|\n______________GUUU_CGCGCAGAA\n3'-UUU...CUAUU____A_________AG-5'\n_____________\|______________\|\n_____________17_____________2\n	109 -- 115	3 -- 9	-18.0761 kcal/mol	2.94526 kcal/mol	1.22934 kcal/mol	102 116@LB@ \| \|@LB@5'-GGA...CGUUU CCGGC...GCG-3'@LB@ CAAA GCGCGUCUU@LB@ \|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ GUUU CGCGCAGAA@LB@3'-UUU...CUAUU A AG-5'@LB@ \| \|@LB@ 17 2@LB@@LB@@LB@Energy -13.90 kcal/mol@LB@Hybridization Energy -18.0761 kcal/mol@LB@Unfolding Energy - mRNA 2.94526 kcal/mol@LB@Unfolding Energy - sRNA 1.22934 kcal/mol@LB@@LB@Position - mRNA 103 -- 115@LB@Position - sRNA 3 -- 16@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 3 -- 9@LB@
50	0.004415	0.299	b0752	zitB	-14.03	0.023662	243 -- 258	5 -- 21	zinc efflux system	_____________242_______________259\n_____________\|_________________\|\n5'-CGC...CAGAA____________U____GCUUG...UGG-3'\n______________GAUAAUAA_UGC_CGUC\n______________\|\|\|\|\|:\|\|_\|\|\|_\|\|\|\|\n______________CUAUUGUU_ACG_GCAG\n3'-UUU...UACUA________U___C____AAAG-5'\n_____________\|_________________\|\n_____________22________________4\n	244 -- 250	14 -- 20	-17.36 kcal/mol	2.08049 kcal/mol	1.24955 kcal/mol	242 259@LB@ \| \|@LB@5'-CGC...CAGAA U GCUUG...UGG-3'@LB@ GAUAAUAA UGC CGUC@LB@ \|\|\|\|\|:\|\| \|\|\| \|\|\|\|@LB@ CUAUUGUU ACG GCAG@LB@3'-UUU...UACUA U C AAAG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -14.03 kcal/mol@LB@Hybridization Energy -17.36 kcal/mol@LB@Unfolding Energy - mRNA 2.08049 kcal/mol@LB@Unfolding Energy - sRNA 1.24955 kcal/mol@LB@@LB@Position - mRNA 243 -- 258@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 14 -- 20@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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