Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
112	0.01125	0.3104	b2515	ispG	-10.24	0.110168	132 -- 140	4 -- 12	1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase	_____________131_______141\n_____________\|_________\|\n5'-CGU...CAGGU_________GACCC...UGA-3'\n______________UGCGCGUCU\n______________\|\|\|\|\|\|\|\|\|\n______________ACGCGCAGA\n3'-UUU...UGUUU_________AAG-5'\n_____________\|_________\|\n_____________13________3\n	134 -- 140	4 -- 10	-16.4542 kcal/mol	5.04928 kcal/mol	1.16019 kcal/mol	131 141@LB@ \| \|@LB@5'-CGU...CAGGU GACCC...UGA-3'@LB@ UGCGCGUCU@LB@ \|\|\|\|\|\|\|\|\|@LB@ ACGCGCAGA@LB@3'-UUU...UGUUU AAG-5'@LB@ \| \|@LB@ 13 3@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -16.4542 kcal/mol@LB@Unfolding Energy - mRNA 5.04928 kcal/mol@LB@Unfolding Energy - sRNA 1.16019 kcal/mol@LB@@LB@Position - mRNA 132 -- 140@LB@Position - sRNA 4 -- 12@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 4 -- 10@LB@
46	0.003408	0.2484	b2518	ndk	-12.77	0.039848	1 -- 16	7 -- 21	multifunctional nucleoside diphosphate kinase and apyrimidinic endonuclease and 3'-phosphodiesterase	______________1_______________17\n______________\|_______________\|\n___________5'-___________A____GGGCA...AAA-3'\n______________GGUGAUGGAUG_CGUG\n______________\|:\|:\|:::\|\|\|_\|\|:\|\n______________CUAUUGUUUAC_GCGC\n3'-UUU...UACUA________________AGAAAG-5'\n_____________\|________________\|\n_____________22_______________6\n	5 -- 11	11 -- 17	-16.3595 kcal/mol	2.34366 kcal/mol	1.24179 kcal/mol	1 17@LB@ \| \|@LB@ 5'- A GGGCA...AAA-3'@LB@ GGUGAUGGAUG CGUG@LB@ \|:\|:\|:::\|\|\| \|\|:\|@LB@ CUAUUGUUUAC GCGC@LB@3'-UUU...UACUA AGAAAG-5'@LB@ \| \|@LB@ 22 6@LB@@LB@@LB@Energy -12.77 kcal/mol@LB@Hybridization Energy -16.3595 kcal/mol@LB@Unfolding Energy - mRNA 2.34366 kcal/mol@LB@Unfolding Energy - sRNA 1.24179 kcal/mol@LB@@LB@Position - mRNA 1 -- 16@LB@Position - sRNA 7 -- 21@LB@Position Seed - mRNA 5 -- 11@LB@Position Seed - sRNA 11 -- 17@LB@
72	0.006739	0.3087	b2520	yfhM	-8.19	0.237735	190 -- 217	6 -- 30	bacterial alpha2-macroglobulin colonization factor ECAM; anti-host protease defense factor; periplasmic inner membrane-anchored lipoprotein	_____________189__________________________218\n_____________\|____________________________\|\n5'-CUC...GUGGC________AAA____AA____UA_____AGCCG...CUU-3'\n______________AGGGAUGA___AUGA__AAGU__CGCGU\n______________\|\|\|\|\|\|\|\|___\|\|:\|__\|\|:\|__\|\|\|\|\|\n______________UCCCUACU___UAUU__UUUA__GCGCA\n3'-UUU...UUAAG________AC_____G_____C______GAAAG-5'\n_____________\|____________________________\|\n_____________31___________________________5\n	191 -- 197	23 -- 29	-19.496 kcal/mol	3.87755 kcal/mol	7.4255 kcal/mol	189 218@LB@ \| \|@LB@5'-CUC...GUGGC AAA AA UA AGCCG...CUU-3'@LB@ AGGGAUGA AUGA AAGU CGCGU@LB@ \|\|\|\|\|\|\|\| \|\|:\| \|\|:\| \|\|\|\|\|@LB@ UCCCUACU UAUU UUUA GCGCA@LB@3'-UUU...UUAAG AC G C GAAAG-5'@LB@ \| \|@LB@ 31 5@LB@@LB@@LB@Energy -8.19 kcal/mol@LB@Hybridization Energy -19.496 kcal/mol@LB@Unfolding Energy - mRNA 3.87755 kcal/mol@LB@Unfolding Energy - sRNA 7.4255 kcal/mol@LB@@LB@Position - mRNA 190 -- 217@LB@Position - sRNA 6 -- 30@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 23 -- 29@LB@
36	0.002407	0.2206	b2550	yphH	-13.17	0.033778	238 -- 251	7 -- 20	putative DNA-binding transcriptional regulator	_____________237____________252\n_____________\|______________\|\n5'-AGG...CCACC______________AUUCU...UGG-3'\n______________AUAACAAAUGCGUG\n______________\|\|\|\|\|\|\|\|\|\|\|\|:\|\n______________UAUUGUUUACGCGC\n3'-UUU...ACUAC______________AGAAAG-5'\n_____________\|______________\|\n_____________21_____________6\n	245 -- 251	7 -- 13	-19.0929 kcal/mol	4.67933 kcal/mol	1.23906 kcal/mol	237 252@LB@ \| \|@LB@5'-AGG...CCACC AUUCU...UGG-3'@LB@ AUAACAAAUGCGUG@LB@ \|\|\|\|\|\|\|\|\|\|\|\|:\|@LB@ UAUUGUUUACGCGC@LB@3'-UUU...ACUAC AGAAAG-5'@LB@ \| \|@LB@ 21 6@LB@@LB@@LB@Energy -13.17 kcal/mol@LB@Hybridization Energy -19.0929 kcal/mol@LB@Unfolding Energy - mRNA 4.67933 kcal/mol@LB@Unfolding Energy - sRNA 1.23906 kcal/mol@LB@@LB@Position - mRNA 238 -- 251@LB@Position - sRNA 7 -- 20@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 7 -- 13@LB@
13	0.0003435	0.08948	b2551	glyA	-14.51	0.019315	147 -- 213	7 -- 69	serine hydroxymethyltransferase	_____________146____________________________________________________________________214\n_____________\|______________________________________________________________________\|\n5'-CAA...CCAAC___A______UAUAAA_______A________GUUAG___________A____CG____U__________AAAUG...UCG-3'\n______________AGG_CCGC_C______GGCCAAA_AUUUUAUU_____CUGAG_UCAGG_GAUG__GAUG_UAAA_GCGUG\n______________\|\|\|_\|\|:\|_\|______\|\|\|\|\|\|\|_\|\|\|\|:\|\|:_____\|\|\|\|:_\|\|\|\|\|_\|\|\|\|__\|\|\|:_:\|\|\|_\|\|\|:\|\n______________UCC_GGUG_G______CCGGUUU_UAAAGUAG_____GACUU_AGUCC_CUAC__CUAU_GUUU_CGCGC\n________3'-UUU________A_CACUCA________________A_________A__________UA____U____A_____AGAAAG-5'\n_____________\|______________________________________________________________________\|\n_____________70_____________________________________________________________________6\n	162 -- 168	48 -- 54	-52.4461 kcal/mol	16.168 kcal/mol	21.7638 kcal/mol	146 214@LB@ \| \|@LB@5'-CAA...CCAAC A UAUAAA A GUUAG A CG U AAAUG...UCG-3'@LB@ AGG CCGC C GGCCAAA AUUUUAUU CUGAG UCAGG GAUG GAUG UAAA GCGUG@LB@ \|\|\| \|\|:\| \| \|\|\|\|\|\|\| \|\|\|\|:\|\|: \|\|\|\|: \|\|\|\|\| \|\|\|\| \|\|\|: :\|\|\| \|\|\|:\|@LB@ UCC GGUG G CCGGUUU UAAAGUAG GACUU AGUCC CUAC CUAU GUUU CGCGC@LB@ 3'-UUU A CACUCA A A UA U A AGAAAG-5'@LB@ \| \|@LB@ 70 6@LB@@LB@@LB@Energy -14.51 kcal/mol@LB@Hybridization Energy -52.4461 kcal/mol@LB@Unfolding Energy - mRNA 16.168 kcal/mol@LB@Unfolding Energy - sRNA 21.7638 kcal/mol@LB@@LB@Position - mRNA 147 -- 213@LB@Position - sRNA 7 -- 69@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 48 -- 54@LB@
91	0.009339	0.3104	b2562	yfhL	-11.62	0.063873	146 -- 160	6 -- 20	putative 4Fe-4S cluster-containing protein	_____________145_____________161\n_____________\|_______________\|\n5'-AGC...UCUGA____A__________AUAAU...UUA-3'\n______________GUAA_GAAUGCGCGU\n______________:\|\|\|_:\|\|\|\|\|\|\|\|\|\n______________UAUU_UUUACGCGCA\n3'-UUU...ACUAC____G__________GAAAG-5'\n_____________\|_______________\|\n_____________21______________5\n	154 -- 160	6 -- 12	-16.8242 kcal/mol	3.96482 kcal/mol	1.24244 kcal/mol	145 161@LB@ \| \|@LB@5'-AGC...UCUGA A AUAAU...UUA-3'@LB@ GUAA GAAUGCGCGU@LB@ :\|\|\| :\|\|\|\|\|\|\|\|\|@LB@ UAUU UUUACGCGCA@LB@3'-UUU...ACUAC G GAAAG-5'@LB@ \| \|@LB@ 21 5@LB@@LB@@LB@Energy -11.62 kcal/mol@LB@Hybridization Energy -16.8242 kcal/mol@LB@Unfolding Energy - mRNA 3.96482 kcal/mol@LB@Unfolding Energy - sRNA 1.24244 kcal/mol@LB@@LB@Position - mRNA 146 -- 160@LB@Position - sRNA 6 -- 20@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 6 -- 12@LB@
108	0.01118	0.3104	b2565	recO	-9.71	0.135448	294 -- 300	5 -- 11	gap repair protein	_____________293____300\n_____________\|______\|\n5'-GAA...GGCGC_______-3'\n______________GUGCGUC\n______________\|:\|\|\|\|\|\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	294 -- 300	5 -- 11	-11.8762 kcal/mol	1.05403 kcal/mol	1.11145 kcal/mol	293 300@LB@ \| \|@LB@5'-GAA...GGCGC -3'@LB@ GUGCGUC@LB@ \|:\|\|\|\|\|@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -9.71 kcal/mol@LB@Hybridization Energy -11.8762 kcal/mol@LB@Unfolding Energy - mRNA 1.05403 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 294 -- 300@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 294 -- 300@LB@Position Seed - sRNA 5 -- 11@LB@
118	0.01199	0.3104	b2573	rpoE	-10.72	0.091300	77 -- 87	8 -- 18	RNA polymerase sigma E factor	_____________76__________88\n_____________\|___________\|\n5'-ACA...ACAAA___________UACGG...UGG-3'\n______________AACAGAUGCGU\n______________\|\|\|\|:\|\|\|\|\|:\n______________UUGUUUACGCG\n3'-UUU...UACUA___________CAGAAAG-5'\n_____________\|___________\|\n_____________19__________7\n	81 -- 87	8 -- 14	-15.0531 kcal/mol	3.12642 kcal/mol	1.20363 kcal/mol	76 88@LB@ \| \|@LB@5'-ACA...ACAAA UACGG...UGG-3'@LB@ AACAGAUGCGU@LB@ \|\|\|\|:\|\|\|\|\|:@LB@ UUGUUUACGCG@LB@3'-UUU...UACUA CAGAAAG-5'@LB@ \| \|@LB@ 19 7@LB@@LB@@LB@Energy -10.72 kcal/mol@LB@Hybridization Energy -15.0531 kcal/mol@LB@Unfolding Energy - mRNA 3.12642 kcal/mol@LB@Unfolding Energy - sRNA 1.20363 kcal/mol@LB@@LB@Position - mRNA 77 -- 87@LB@Position - sRNA 8 -- 18@LB@Position Seed - mRNA 81 -- 87@LB@Position Seed - sRNA 8 -- 14@LB@
84	0.008129	0.3104	b2579	grcA	-9.71	0.135356	279 -- 291	5 -- 18	autonomous glycyl radical cofactor	_____________278____________292\n_____________\|______________\|\n5'-UUG...AAAAA______________GCAUC...UUG-3'\n______________GGCGAA_GCGCGUU\n______________::\|:\|\|_\|\|\|\|\|\|:\n______________UUGUUU_CGCGCAG\n3'-UUU...UACUA______A_______AAAG-5'\n_____________\|______________\|\n_____________19_____________4\n	285 -- 291	5 -- 11	-16.0784 kcal/mol	5.14159 kcal/mol	1.2243 kcal/mol	278 292@LB@ \| \|@LB@5'-UUG...AAAAA GCAUC...UUG-3'@LB@ GGCGAA GCGCGUU@LB@ ::\|:\|\| \|\|\|\|\|\|:@LB@ UUGUUU CGCGCAG@LB@3'-UUU...UACUA A AAAG-5'@LB@ \| \|@LB@ 19 4@LB@@LB@@LB@Energy -9.71 kcal/mol@LB@Hybridization Energy -16.0784 kcal/mol@LB@Unfolding Energy - mRNA 5.14159 kcal/mol@LB@Unfolding Energy - sRNA 1.2243 kcal/mol@LB@@LB@Position - mRNA 279 -- 291@LB@Position - sRNA 5 -- 18@LB@Position Seed - mRNA 285 -- 291@LB@Position Seed - sRNA 5 -- 11@LB@
10	0.0002413	0.08171	b2594	rluD	-15.70	0.011720	48 -- 64	5 -- 21	23S rRNA pseudouridine(1911 1915 1917) synthase	_____________47________________65\n_____________\|_________________\|\n5'-CCC...CACCA____UUUC_________AUGAC...GUU-3'\n______________GAUA____AUGCGCGUC\n______________\|\|\|\|____\|\|\|\|\|\|\|\|\|\n______________CUAU____UACGCGCAG\n3'-UUU...UACUA____UGUU_________AAAG-5'\n_____________\|_________________\|\n_____________22________________4\n	58 -- 64	5 -- 11	-19.7285 kcal/mol	2.7828 kcal/mol	1.24955 kcal/mol	47 65@LB@ \| \|@LB@5'-CCC...CACCA UUUC AUGAC...GUU-3'@LB@ GAUA AUGCGCGUC@LB@ \|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ CUAU UACGCGCAG@LB@3'-UUU...UACUA UGUU AAAG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -15.70 kcal/mol@LB@Hybridization Energy -19.7285 kcal/mol@LB@Unfolding Energy - mRNA 2.7828 kcal/mol@LB@Unfolding Energy - sRNA 1.24955 kcal/mol@LB@@LB@Position - mRNA 48 -- 64@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 58 -- 64@LB@Position Seed - sRNA 5 -- 11@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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