Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
134	0.01294	0.3104	b2234	nrdA	-13.57	0.028625	217 -- 231	7 -- 22	ribonucleoside-diphosphate reductase 1 alpha subunit	_____________216______________232\n_____________\|________________\|\n5'-GAC...UCUGC________________ACGGU...UGC-3'\n______________UGGUGACAAA_GCGCG\n______________\|\|:\|:\|\|\|\|\|_\|\|\|\|\|\n______________ACUAUUGUUU_CGCGC\n3'-UUU...CUACU__________A_____AGAAAG-5'\n_____________\|________________\|\n_____________23_______________6\n	220 -- 226	13 -- 19	-19.6051 kcal/mol	4.78775 kcal/mol	1.24379 kcal/mol	216 232@LB@ \| \|@LB@5'-GAC...UCUGC ACGGU...UGC-3'@LB@ UGGUGACAAA GCGCG@LB@ \|\|:\|:\|\|\|\|\| \|\|\|\|\|@LB@ ACUAUUGUUU CGCGC@LB@3'-UUU...CUACU A AGAAAG-5'@LB@ \| \|@LB@ 23 6@LB@@LB@@LB@Energy -13.57 kcal/mol@LB@Hybridization Energy -19.6051 kcal/mol@LB@Unfolding Energy - mRNA 4.78775 kcal/mol@LB@Unfolding Energy - sRNA 1.24379 kcal/mol@LB@@LB@Position - mRNA 217 -- 231@LB@Position - sRNA 7 -- 22@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 13 -- 19@LB@
11	0.0003246	0.08948	b2299	yfcD	-11.11	0.078371	283 -- 295	5 -- 18	putative NUDIX hydrolase	_____________282____________296\n_____________\|______________\|\n5'-AGA...CCGGG__________G___ACAGU-3'\n______________AACAAAUGCG_G_C\n______________\|\|\|\|\|\|\|\|\|\|_\|_\|\n______________UUGUUUACGC_C_G\n3'-UUU...UACUA__________G_A_AAAG-5'\n_____________\|______________\|\n_____________19_____________4\n	286 -- 292	9 -- 15	-15.7313 kcal/mol	3.39963 kcal/mol	1.2243 kcal/mol	282 296@LB@ \| \|@LB@5'-AGA...CCGGG G ACAGU-3'@LB@ AACAAAUGCG G C@LB@ \|\|\|\|\|\|\|\|\|\| \| \|@LB@ UUGUUUACGC C G@LB@3'-UUU...UACUA G A AAAG-5'@LB@ \| \|@LB@ 19 4@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -15.7313 kcal/mol@LB@Unfolding Energy - mRNA 3.39963 kcal/mol@LB@Unfolding Energy - sRNA 1.2243 kcal/mol@LB@@LB@Position - mRNA 283 -- 295@LB@Position - sRNA 5 -- 18@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 9 -- 15@LB@
28	0.001743	0.2094	b2308	hisQ	-15.00	0.015715	1 -- 16	7 -- 21	histidine ABC transporter permease	______________1_______________17\n______________\|_______________\|\n___________5'-_________C______AAGCA...UUG-3'\n______________GAUAACGAA_UGCGCG\n______________\|\|\|\|\|\|:\|\|_\|\|\|\|\|\|\n______________CUAUUGUUU_ACGCGC\n3'-UUU...UACUA________________AGAAAG-5'\n_____________\|________________\|\n_____________22_______________6\n	3 -- 9	13 -- 19	-21.0778 kcal/mol	4.83206 kcal/mol	1.24179 kcal/mol	1 17@LB@ \| \|@LB@ 5'- C AAGCA...UUG-3'@LB@ GAUAACGAA UGCGCG@LB@ \|\|\|\|\|\|:\|\| \|\|\|\|\|\|@LB@ CUAUUGUUU ACGCGC@LB@3'-UUU...UACUA AGAAAG-5'@LB@ \| \|@LB@ 22 6@LB@@LB@@LB@Energy -15.00 kcal/mol@LB@Hybridization Energy -21.0778 kcal/mol@LB@Unfolding Energy - mRNA 4.83206 kcal/mol@LB@Unfolding Energy - sRNA 1.24179 kcal/mol@LB@@LB@Position - mRNA 1 -- 16@LB@Position - sRNA 7 -- 21@LB@Position Seed - mRNA 3 -- 9@LB@Position Seed - sRNA 13 -- 19@LB@
148	0.01421	0.3104	b2379	alaC	-8.54	0.209762	290 -- 297	5 -- 12	glutamate-pyruvate aminotransferase; glutamic-pyruvic transaminase (GPT); alanine transaminase	_____________289______298\n_____________\|________\|\n5'-UGU...AUGGC________GGC-3'\n______________UGCGCGUC\n______________\|\|\|\|\|\|\|\|\n______________ACGCGCAG\n3'-UUU...UGUUU________AAAG-5'\n_____________\|________\|\n_____________13_______4\n	291 -- 297	5 -- 11	-14.909 kcal/mol	5.25295 kcal/mol	1.11508 kcal/mol	289 298@LB@ \| \|@LB@5'-UGU...AUGGC GGC-3'@LB@ UGCGCGUC@LB@ \|\|\|\|\|\|\|\|@LB@ ACGCGCAG@LB@3'-UUU...UGUUU AAAG-5'@LB@ \| \|@LB@ 13 4@LB@@LB@@LB@Energy -8.54 kcal/mol@LB@Hybridization Energy -14.909 kcal/mol@LB@Unfolding Energy - mRNA 5.25295 kcal/mol@LB@Unfolding Energy - sRNA 1.11508 kcal/mol@LB@@LB@Position - mRNA 290 -- 297@LB@Position - sRNA 5 -- 12@LB@Position Seed - mRNA 291 -- 297@LB@Position Seed - sRNA 5 -- 11@LB@
140	0.01344	0.3104	b2388	glk	-4.25	0.738023	201 -- 214	10 -- 20	glucokinase	_____________200____________215\n_____________\|______________\|\n5'-CUA...GAAGA_______AGU____AUUAG...AUU-3'\n______________AUGACAA___AUGC\n______________\|\|:\|\|\|\|___\|\|\|\|\n______________UAUUGUU___UACG\n3'-UUU...ACUAC______________CGCAG...AAG-5'\n_____________\|______________\|\n_____________21_____________9\n	201 -- 207	14 -- 20	-8.41213 kcal/mol	2.93934 kcal/mol	1.22774 kcal/mol	200 215@LB@ \| \|@LB@5'-CUA...GAAGA AGU AUUAG...AUU-3'@LB@ AUGACAA AUGC@LB@ \|\|:\|\|\|\| \|\|\|\|@LB@ UAUUGUU UACG@LB@3'-UUU...ACUAC CGCAG...AAG-5'@LB@ \| \|@LB@ 21 9@LB@@LB@@LB@Energy -4.25 kcal/mol@LB@Hybridization Energy -8.41213 kcal/mol@LB@Unfolding Energy - mRNA 2.93934 kcal/mol@LB@Unfolding Energy - sRNA 1.22774 kcal/mol@LB@@LB@Position - mRNA 201 -- 214@LB@Position - sRNA 10 -- 20@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 14 -- 20@LB@
80	0.007305	0.3087	b2392	mntH	-9.52	0.145914	200 -- 218	5 -- 21	manganese/divalent cation transporter	_____________199_________________219\n_____________\|___________________\|\n5'-GCU...CACAA_________CUAU______GAGAG...UCG-3'\n______________GAUGACGAA____CGCGUU\n______________\|\|\|:\|\|:\|\|____\|\|\|\|\|:\n______________CUAUUGUUU____GCGCAG\n3'-UUU...UACUA_________AC________AAAG-5'\n_____________\|___________________\|\n_____________22__________________4\n	202 -- 208	13 -- 19	-16.4824 kcal/mol	5.71706 kcal/mol	1.24955 kcal/mol	199 219@LB@ \| \|@LB@5'-GCU...CACAA CUAU GAGAG...UCG-3'@LB@ GAUGACGAA CGCGUU@LB@ \|\|\|:\|\|:\|\| \|\|\|\|\|:@LB@ CUAUUGUUU GCGCAG@LB@3'-UUU...UACUA AC AAAG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -9.52 kcal/mol@LB@Hybridization Energy -16.4824 kcal/mol@LB@Unfolding Energy - mRNA 5.71706 kcal/mol@LB@Unfolding Energy - sRNA 1.24955 kcal/mol@LB@@LB@Position - mRNA 200 -- 218@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 13 -- 19@LB@
37	0.00241	0.2206	b2455	eutE	-11.61	0.064061	286 -- 298	3 -- 15	aldehyde oxidoreductase ethanolamine utilization protein	_____________285___________299\n_____________\|_____________\|\n5'-GGU...UCAUG_____G_______CG-3'\n______________AGAUG_GCGUUUU\n______________\|:\|\|\|_\|\|\|\|:\|\|\n______________UUUAC_CGCAGAA\n3'-UUU...UAUUG_____G_______AG-5'\n_____________\|_____________\|\n_____________16____________2\n	292 -- 298	3 -- 9	-14.5549 kcal/mol	1.73631 kcal/mol	1.20903 kcal/mol	285 299@LB@ \| \|@LB@5'-GGU...UCAUG G CG-3'@LB@ AGAUG GCGUUUU@LB@ \|:\|\|\| \|\|\|\|:\|\|@LB@ UUUAC CGCAGAA@LB@3'-UUU...UAUUG G AG-5'@LB@ \| \|@LB@ 16 2@LB@@LB@@LB@Energy -11.61 kcal/mol@LB@Hybridization Energy -14.5549 kcal/mol@LB@Unfolding Energy - mRNA 1.73631 kcal/mol@LB@Unfolding Energy - sRNA 1.20903 kcal/mol@LB@@LB@Position - mRNA 286 -- 298@LB@Position - sRNA 3 -- 15@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 3 -- 9@LB@
74	0.006875	0.3087	b2474	tmcA	-10.30	0.108021	196 -- 222	5 -- 31	elongator methionine tRNA (ac4C34) acetyltransferase	_____________195____________________________223\n_____________\|______________________________\|\n5'-GAU...CGGGG_________C___AC____________U__ACACA...UUG-3'\n______________CAGGGAUGG_UGA__UGAC___UGCGC_UC\n______________\|\|\|\|\|\|\|\|:_\|\|\|__\|:\|\|___\|\|\|\|\|_\|\|\n______________GUCCCUACU_ACU__AUUG___ACGCG_AG\n3'-UUU...CUUAA___________________UUU_____C__AAAG-5'\n_____________\|______________________________\|\n_____________32_____________________________4\n	198 -- 204	23 -- 29	-27.934 kcal/mol	9.88036 kcal/mol	7.7585 kcal/mol	195 223@LB@ \| \|@LB@5'-GAU...CGGGG C AC U ACACA...UUG-3'@LB@ CAGGGAUGG UGA UGAC UGCGC UC@LB@ \|\|\|\|\|\|\|\|: \|\|\| \|:\|\| \|\|\|\|\| \|\|@LB@ GUCCCUACU ACU AUUG ACGCG AG@LB@3'-UUU...CUUAA UUU C AAAG-5'@LB@ \| \|@LB@ 32 4@LB@@LB@@LB@Energy -10.30 kcal/mol@LB@Hybridization Energy -27.934 kcal/mol@LB@Unfolding Energy - mRNA 9.88036 kcal/mol@LB@Unfolding Energy - sRNA 7.7585 kcal/mol@LB@@LB@Position - mRNA 196 -- 222@LB@Position - sRNA 5 -- 31@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 23 -- 29@LB@
159	0.01499	0.3104	b2476	purC	-11.66	0.062723	28 -- 49	4 -- 22	phosphoribosylaminoimidazole-succinocarboxamide synthetase	_____________27_____________________50\n_____________\|______________________\|\n5'-GGA...UGAUA_____C____AC__________GCAGG...CAG-3'\n______________UGAUA_GCAA__GUGUGCGUCU\n______________\|\|\|\|\|_:\|\|\|__:\|\|:\|\|\|\|\|\|\n______________ACUAU_UGUU__UACGCGCAGA\n3'-UUU...CUACU______________________AAG-5'\n_____________\|______________________\|\n_____________23_____________________3\n	43 -- 49	4 -- 10	-20.6907 kcal/mol	7.77162 kcal/mol	1.2572 kcal/mol	27 50@LB@ \| \|@LB@5'-GGA...UGAUA C AC GCAGG...CAG-3'@LB@ UGAUA GCAA GUGUGCGUCU@LB@ \|\|\|\|\| :\|\|\| :\|\|:\|\|\|\|\|\|@LB@ ACUAU UGUU UACGCGCAGA@LB@3'-UUU...CUACU AAG-5'@LB@ \| \|@LB@ 23 3@LB@@LB@@LB@Energy -11.66 kcal/mol@LB@Hybridization Energy -20.6907 kcal/mol@LB@Unfolding Energy - mRNA 7.77162 kcal/mol@LB@Unfolding Energy - sRNA 1.2572 kcal/mol@LB@@LB@Position - mRNA 28 -- 49@LB@Position - sRNA 4 -- 22@LB@Position Seed - mRNA 43 -- 49@LB@Position Seed - sRNA 4 -- 10@LB@
106	0.0109	0.3104	b2510	yfgJ	-10.53	0.098521	243 -- 261	5 -- 21	DUF1407 family protein	_____________242_________________262\n_____________\|___________________\|\n5'-UAA...AUCAG________CC_________GCCGA...UUU-3'\n______________GAUAACGG__AUGCGCGUU\n______________\|\|\|\|\|\|::__\|\|\|\|\|\|\|\|:\n______________CUAUUGUU__UACGCGCAG\n3'-UUU...UACUA___________________AAAG-5'\n_____________\|___________________\|\n_____________22__________________4\n	255 -- 261	5 -- 11	-20.9599 kcal/mol	9.18032 kcal/mol	1.24955 kcal/mol	242 262@LB@ \| \|@LB@5'-UAA...AUCAG CC GCCGA...UUU-3'@LB@ GAUAACGG AUGCGCGUU@LB@ \|\|\|\|\|\|:: \|\|\|\|\|\|\|\|:@LB@ CUAUUGUU UACGCGCAG@LB@3'-UUU...UACUA AAAG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -10.53 kcal/mol@LB@Hybridization Energy -20.9599 kcal/mol@LB@Unfolding Energy - mRNA 9.18032 kcal/mol@LB@Unfolding Energy - sRNA 1.24955 kcal/mol@LB@@LB@Position - mRNA 243 -- 261@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 255 -- 261@LB@Position Seed - sRNA 5 -- 11@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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