Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
154	0.01456	0.3104	b2048	cpsG	-9.02	0.175967	42 -- 71	4 -- 44	phosphomannomutase	_____________41________________________________________72\n_____________\|_________________________________________\|\n5'-GGG...GGCUC___________GA___________UG_______________CGCGG...GCG-3'\n______________UUAUCUC_GAA_____GGAUGAUG_________GUGCGUUU\n______________\|:\|\|\|\|\|_\|\|\|_____\|\|\|\|\|\|\|\|_________\|:\|\|\|\|:\|\n______________AGUAGAG_CUU_____CCUACUAC_________CGCGCAGA\n3'-UUU...UUUAA_______A___AAGUC________UAUUGUUUA________AAG-5'\n_____________\|_________________________________________\|\n_____________45________________________________________3\n	65 -- 71	4 -- 10	-28.9111 kcal/mol	10.4373 kcal/mol	9.45546 kcal/mol	41 72@LB@ \| \|@LB@5'-GGG...GGCUC GA UG CGCGG...GCG-3'@LB@ UUAUCUC GAA GGAUGAUG GUGCGUUU@LB@ \|:\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|\| \|:\|\|\|\|:\|@LB@ AGUAGAG CUU CCUACUAC CGCGCAGA@LB@3'-UUU...UUUAA A AAGUC UAUUGUUUA AAG-5'@LB@ \| \|@LB@ 45 3@LB@@LB@@LB@Energy -9.02 kcal/mol@LB@Hybridization Energy -28.9111 kcal/mol@LB@Unfolding Energy - mRNA 10.4373 kcal/mol@LB@Unfolding Energy - sRNA 9.45546 kcal/mol@LB@@LB@Position - mRNA 42 -- 71@LB@Position - sRNA 4 -- 44@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 4 -- 10@LB@
184	0.01824	0.3104	b2055	wcaE	-6.17	0.461247	221 -- 230	4 -- 12	putative glycosyl transferase	_____________220________231\n_____________\|__________\|\n5'-ACU...AUCAC__U_______CGUAA...GCU-3'\n______________UG_CGCGUUU\n______________\|\|_\|\|\|\|\|:\|\n______________AC_GCGCAGA\n3'-UUU...UGUUU__________AAG-5'\n_____________\|__________\|\n_____________13_________3\n	224 -- 230	4 -- 10	-10.1102 kcal/mol	2.78254 kcal/mol	1.16019 kcal/mol	220 231@LB@ \| \|@LB@5'-ACU...AUCAC U CGUAA...GCU-3'@LB@ UG CGCGUUU@LB@ \|\| \|\|\|\|\|:\|@LB@ AC GCGCAGA@LB@3'-UUU...UGUUU AAG-5'@LB@ \| \|@LB@ 13 3@LB@@LB@@LB@Energy -6.17 kcal/mol@LB@Hybridization Energy -10.1102 kcal/mol@LB@Unfolding Energy - mRNA 2.78254 kcal/mol@LB@Unfolding Energy - sRNA 1.16019 kcal/mol@LB@@LB@Position - mRNA 221 -- 230@LB@Position - sRNA 4 -- 12@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 4 -- 10@LB@
60	0.00555	0.3084	b2062	wza	-10.85	0.086669	18 -- 25	4 -- 11	colanic acid export protein; outer membrane auxillary lipoprotein	_____________17_______26\n_____________\|________\|\n5'-AAA...CGGUA________GGAUG...GCA-3'\n______________GCGUGUCU\n______________\|\|\|:\|\|\|\|\n______________CGCGCAGA\n3'-UUU...GUUUA________AAG-5'\n_____________\|________\|\n_____________12_______3\n	19 -- 25	4 -- 10	-13.9362 kcal/mol	1.92535 kcal/mol	1.15639 kcal/mol	17 26@LB@ \| \|@LB@5'-AAA...CGGUA GGAUG...GCA-3'@LB@ GCGUGUCU@LB@ \|\|\|:\|\|\|\|@LB@ CGCGCAGA@LB@3'-UUU...GUUUA AAG-5'@LB@ \| \|@LB@ 12 3@LB@@LB@@LB@Energy -10.85 kcal/mol@LB@Hybridization Energy -13.9362 kcal/mol@LB@Unfolding Energy - mRNA 1.92535 kcal/mol@LB@Unfolding Energy - sRNA 1.15639 kcal/mol@LB@@LB@Position - mRNA 18 -- 25@LB@Position - sRNA 4 -- 11@LB@Position Seed - mRNA 19 -- 25@LB@Position Seed - sRNA 4 -- 10@LB@
18	0.00061	0.1147	b2126	yehU	-11.17	0.076396	235 -- 252	1 -- 18	inner membrane putative sensory kinase in two-component system with YehT	_____________234________________253\n_____________\|__________________\|\n5'-GUC...GCUUC__________________UAGUC...UAA-3'\n______________AGCAGAUGUGCGUUUUUU\n______________\|:\|\|:\|\|\|:\|\|\|\|:\|\|\|:\n______________UUGUUUACGCGCAGAAAG\n3'-UUU...UACUA__________________-5'\n_____________\|_________________\|\n_____________19________________1\n	246 -- 252	1 -- 7	-22.3117 kcal/mol	9.89973 kcal/mol	1.24077 kcal/mol	234 253@LB@ \| \|@LB@5'-GUC...GCUUC UAGUC...UAA-3'@LB@ AGCAGAUGUGCGUUUUUU@LB@ \|:\|\|:\|\|\|:\|\|\|\|:\|\|\|:@LB@ UUGUUUACGCGCAGAAAG@LB@3'-UUU...UACUA -5'@LB@ \| \|@LB@ 19 1@LB@@LB@@LB@Energy -11.17 kcal/mol@LB@Hybridization Energy -22.3117 kcal/mol@LB@Unfolding Energy - mRNA 9.89973 kcal/mol@LB@Unfolding Energy - sRNA 1.24077 kcal/mol@LB@@LB@Position - mRNA 235 -- 252@LB@Position - sRNA 1 -- 18@LB@Position Seed - mRNA 246 -- 252@LB@Position Seed - sRNA 1 -- 7@LB@
117	0.01191	0.3104	b2137	yohF	-11.08	0.079147	231 -- 259	5 -- 35	putative oxidoreductase	_____________230_____________________________260\n_____________\|_______________________________\|\n5'-UAA...CCUCC___________C____CAAA___________AUUAC...GGC-3'\n______________GAUUCGGGGAU___GG____GAGUGCGCGUU\n______________:\|\|\|\|:\|\|\|\|\|___\|:____:\|:\|\|\|\|\|\|\|:\n______________UUAAGUCCCUA___CU____UUUACGCGCAG\n3'-UUU...GAGAC___________CUA__AUUG___________AAAG-5'\n_____________\|_______________________________\|\n_____________36______________________________4\n	253 -- 259	5 -- 11	-31.497 kcal/mol	11.2631 kcal/mol	9.15134 kcal/mol	230 260@LB@ \| \|@LB@5'-UAA...CCUCC C CAAA AUUAC...GGC-3'@LB@ GAUUCGGGGAU GG GAGUGCGCGUU@LB@ :\|\|\|\|:\|\|\|\|\| \|: :\|:\|\|\|\|\|\|\|:@LB@ UUAAGUCCCUA CU UUUACGCGCAG@LB@3'-UUU...GAGAC CUA AUUG AAAG-5'@LB@ \| \|@LB@ 36 4@LB@@LB@@LB@Energy -11.08 kcal/mol@LB@Hybridization Energy -31.497 kcal/mol@LB@Unfolding Energy - mRNA 11.2631 kcal/mol@LB@Unfolding Energy - sRNA 9.15134 kcal/mol@LB@@LB@Position - mRNA 231 -- 259@LB@Position - sRNA 5 -- 35@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 5 -- 11@LB@
15	0.000461	0.102	b2140	dusC	-12.07	0.053129	194 -- 211	3 -- 22	tRNA-dihydrouridine synthase C	_____________193__________________212\n_____________\|____________________\|\n5'-AAU...GCGGG_________________G__ACUGG...UUG-3'\n______________UGAUGAU__AUGCGUGU_UU\n______________\|\|\|\|:\|:__\|\|\|\|\|:\|\|_\|\|\n______________ACUAUUG__UACGCGCA_AA\n3'-UUU...CUACU_______UU________G__AG-5'\n_____________\|____________________\|\n_____________23___________________2\n	202 -- 208	6 -- 12	-18.0697 kcal/mol	4.73944 kcal/mol	1.259 kcal/mol	193 212@LB@ \| \|@LB@5'-AAU...GCGGG G ACUGG...UUG-3'@LB@ UGAUGAU AUGCGUGU UU@LB@ \|\|\|\|:\|: \|\|\|\|\|:\|\| \|\|@LB@ ACUAUUG UACGCGCA AA@LB@3'-UUU...CUACU UU G AG-5'@LB@ \| \|@LB@ 23 2@LB@@LB@@LB@Energy -12.07 kcal/mol@LB@Hybridization Energy -18.0697 kcal/mol@LB@Unfolding Energy - mRNA 4.73944 kcal/mol@LB@Unfolding Energy - sRNA 1.259 kcal/mol@LB@@LB@Position - mRNA 194 -- 211@LB@Position - sRNA 3 -- 22@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 6 -- 12@LB@
187	0.01837	0.3104	b2151	galS	-10.59	0.096282	222 -- 228	5 -- 11	galactose- and fucose-inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor	_____________221_____229\n_____________\|_______\|\n5'-UCU...AUGUA_______AGGCU...GUG-3'\n______________GCGCGUC\n______________\|\|\|\|\|\|\|\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	222 -- 228	5 -- 11	-14.6299 kcal/mol	2.93004 kcal/mol	1.11145 kcal/mol	221 229@LB@ \| \|@LB@5'-UCU...AUGUA AGGCU...GUG-3'@LB@ GCGCGUC@LB@ \|\|\|\|\|\|\|@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -10.59 kcal/mol@LB@Hybridization Energy -14.6299 kcal/mol@LB@Unfolding Energy - mRNA 2.93004 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 222 -- 228@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 222 -- 228@LB@Position Seed - sRNA 5 -- 11@LB@
196	0.01921	0.3104	b2167	fruA	-9.92	0.125082	150 -- 163	5 -- 22	fused fructose-specific PTS enzymes: IIBcomponent/IIC components	_____________149________________164\n_____________\|__________________\|\n5'-CGC...CGCAA__________________GACUU...AAA-3'\n______________UGAUGGC____GCGCGUC\n______________\|\|\|\|::\|____\|\|\|\|\|\|\|\n______________ACUAUUG____CGCGCAG\n3'-UUU...CUACU_______UUUA_______AAAG-5'\n_____________\|__________________\|\n_____________23_________________4\n	157 -- 163	5 -- 11	-18.2874 kcal/mol	7.11819 kcal/mol	1.25155 kcal/mol	149 164@LB@ \| \|@LB@5'-CGC...CGCAA GACUU...AAA-3'@LB@ UGAUGGC GCGCGUC@LB@ \|\|\|\|::\| \|\|\|\|\|\|\|@LB@ ACUAUUG CGCGCAG@LB@3'-UUU...CUACU UUUA AAAG-5'@LB@ \| \|@LB@ 23 4@LB@@LB@@LB@Energy -9.92 kcal/mol@LB@Hybridization Energy -18.2874 kcal/mol@LB@Unfolding Energy - mRNA 7.11819 kcal/mol@LB@Unfolding Energy - sRNA 1.25155 kcal/mol@LB@@LB@Position - mRNA 150 -- 163@LB@Position - sRNA 5 -- 22@LB@Position Seed - mRNA 157 -- 163@LB@Position Seed - sRNA 5 -- 11@LB@
61	0.005608	0.3084	b2174	lpxT	-9.09	0.171357	170 -- 179	1 -- 10	lipid A 1-diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1-phosphate phosphotransferase	_____________169________180\n_____________\|__________\|\n5'-GAA...UUGCC__________AGUAA...CGA-3'\n______________UGCGUUUUUC\n______________:\|\|\|\|:\|\|\|\|\n______________GCGCAGAAAG\n3'-UUU...UUUAC__________-5'\n_____________\|_________\|\n_____________11________1\n	173 -- 179	1 -- 7	-13.1644 kcal/mol	2.90849 kcal/mol	1.16635 kcal/mol	169 180@LB@ \| \|@LB@5'-GAA...UUGCC AGUAA...CGA-3'@LB@ UGCGUUUUUC@LB@ :\|\|\|\|:\|\|\|\|@LB@ GCGCAGAAAG@LB@3'-UUU...UUUAC -5'@LB@ \| \|@LB@ 11 1@LB@@LB@@LB@Energy -9.09 kcal/mol@LB@Hybridization Energy -13.1644 kcal/mol@LB@Unfolding Energy - mRNA 2.90849 kcal/mol@LB@Unfolding Energy - sRNA 1.16635 kcal/mol@LB@@LB@Position - mRNA 170 -- 179@LB@Position - sRNA 1 -- 10@LB@Position Seed - mRNA 173 -- 179@LB@Position Seed - sRNA 1 -- 7@LB@
197	0.01929	0.3104	b2185	rplY	-8.58	0.206984	8 -- 20	7 -- 19	50S ribosomal subunit protein L25	_____________7_____________21\n_____________\|_____________\|\n____5'-CCAGUAC_____________AAAAA...GCA-3'\n______________UGGCAGGUGCGUG\n______________\|::\|\|::\|\|\|\|:\|\n______________AUUGUUUACGCGC\n3'-UUU...CUACU_____________AGAAAG-5'\n_____________\|_____________\|\n_____________20____________6\n	14 -- 20	7 -- 13	-17.3397 kcal/mol	7.54262 kcal/mol	1.21943 kcal/mol	7 21@LB@ \| \|@LB@ 5'-CCAGUAC AAAAA...GCA-3'@LB@ UGGCAGGUGCGUG@LB@ \|::\|\|::\|\|\|\|:\|@LB@ AUUGUUUACGCGC@LB@3'-UUU...CUACU AGAAAG-5'@LB@ \| \|@LB@ 20 6@LB@@LB@@LB@Energy -8.58 kcal/mol@LB@Hybridization Energy -17.3397 kcal/mol@LB@Unfolding Energy - mRNA 7.54262 kcal/mol@LB@Unfolding Energy - sRNA 1.21943 kcal/mol@LB@@LB@Position - mRNA 8 -- 20@LB@Position - sRNA 7 -- 19@LB@Position Seed - mRNA 14 -- 20@LB@Position Seed - sRNA 7 -- 13@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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