Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
87	0.008927	0.3104	b0195	tsaA	-8.24	0.234029	220 -- 237	1 -- 18	tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase SAM-dependent		_____________219________________238\n_____________\|__________________\|\n5'-GAA...GUUCG_______AG_____A___GCUCG...ACG-3'\n______________AGCAAAU__GCGUU_UUC\n______________\|:\|\|\|\|\|__\|\|\|\|:_\|\|\|\n______________UUGUUUA__CGCAG_AAG\n3'-UUU...UACUA_______CG_____A___-5'\n_____________\|_________________\|\n_____________19________________1\n	220 -- 226	12 -- 18	-15.6275 kcal/mol	6.14962 kcal/mol	1.24077 kcal/mol	219 238@LB@ \| \|@LB@5'-GAA...GUUCG AG A GCUCG...ACG-3'@LB@ AGCAAAU GCGUU UUC@LB@ \|:\|\|\|\|\| \|\|\|\|: \|\|\|@LB@ UUGUUUA CGCAG AAG@LB@3'-UUU...UACUA CG A -5'@LB@ \| \|@LB@ 19 1@LB@@LB@@LB@Energy -8.24 kcal/mol@LB@Hybridization Energy -15.6275 kcal/mol@LB@Unfolding Energy - mRNA 6.14962 kcal/mol@LB@Unfolding Energy - sRNA 1.24077 kcal/mol@LB@@LB@Position - mRNA 220 -- 237@LB@Position - sRNA 1 -- 18@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 12 -- 18@LB@
190	0.01867	0.3104	b0196	rcsF	-10.27	0.109081	201 -- 211	2 -- 13	putative outer membrane protein		_____________200__________212\n_____________\|____________\|\n5'-AAU...AUACU____________ACCGA...AGC-3'\n______________AUGCGUG_CUUU\n______________\|\|\|\|\|:\|_\|\|\|\|\n______________UACGCGC_GAAA\n3'-UUU...UUGUU_______A____G-5'\n_____________\|____________\|\n_____________14___________1\n	201 -- 207	7 -- 13	-12.8527 kcal/mol	1.40833 kcal/mol	1.17421 kcal/mol	200 212@LB@ \| \|@LB@5'-AAU...AUACU ACCGA...AGC-3'@LB@ AUGCGUG CUUU@LB@ \|\|\|\|\|:\| \|\|\|\|@LB@ UACGCGC GAAA@LB@3'-UUU...UUGUU A G-5'@LB@ \| \|@LB@ 14 1@LB@@LB@@LB@Energy -10.27 kcal/mol@LB@Hybridization Energy -12.8527 kcal/mol@LB@Unfolding Energy - mRNA 1.40833 kcal/mol@LB@Unfolding Energy - sRNA 1.17421 kcal/mol@LB@@LB@Position - mRNA 201 -- 211@LB@Position - sRNA 2 -- 13@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 7 -- 13@LB@
66	0.006019	0.3087	b0223	yafJ	-7.05	0.351504	68 -- 78	1 -- 10	type 2 glutamine amidotransferase family protein		_____________67__________79\n_____________\|___________\|\n5'-GCG...GCUGA________A__AUGCC...AAG-3'\n______________CGCGUCUU_UC\n______________\|\|\|\|\|\|\|\|_\|\|\n______________GCGCAGAA_AG\n3'-UUU...UUUAC___________-5'\n_____________\|__________\|\n_____________11_________1\n	69 -- 75	3 -- 9	-12.9748 kcal/mol	4.76165 kcal/mol	1.16635 kcal/mol	67 79@LB@ \| \|@LB@5'-GCG...GCUGA A AUGCC...AAG-3'@LB@ CGCGUCUU UC@LB@ \|\|\|\|\|\|\|\| \|\|@LB@ GCGCAGAA AG@LB@3'-UUU...UUUAC -5'@LB@ \| \|@LB@ 11 1@LB@@LB@@LB@Energy -7.05 kcal/mol@LB@Hybridization Energy -12.9748 kcal/mol@LB@Unfolding Energy - mRNA 4.76165 kcal/mol@LB@Unfolding Energy - sRNA 1.16635 kcal/mol@LB@@LB@Position - mRNA 68 -- 78@LB@Position - sRNA 1 -- 10@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 3 -- 9@LB@
21	0.0009524	0.1536	b0241	phoE	-11.39	0.070016	174 -- 180	5 -- 11	outer membrane phosphoporin protein E	b0929 b2215 b1377	_____________173_____181\n_____________\|_______\|\n5'-AGC...UAUUC_______AAUUA...AUG-3'\n______________GCGCGUU\n______________\|\|\|\|\|\|:\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	174 -- 180	5 -- 11	-12.879 kcal/mol	0.378776 kcal/mol	1.11145 kcal/mol	173 181@LB@ \| \|@LB@5'-AGC...UAUUC AAUUA...AUG-3'@LB@ GCGCGUU@LB@ \|\|\|\|\|\|:@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -11.39 kcal/mol@LB@Hybridization Energy -12.879 kcal/mol@LB@Unfolding Energy - mRNA 0.378776 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 174 -- 180@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 5 -- 11@LB@
179	0.01766	0.3104	b0377	sbmA	-9.49	0.147268	6 -- 15	3 -- 12	peptide antibiotic transporter	b1496	_____________5__________16\n_____________\|__________\|\n______5'-GAUCC_______G__AAUCC...ACU-3'\n______________UGCGCGU_UU\n______________\|\|\|\|\|\|\|_\|\|\n______________ACGCGCA_AA\n3'-UUU...UGUUU_______G__AG-5'\n_____________\|__________\|\n_____________13_________2\n	6 -- 12	6 -- 12	-14.1156 kcal/mol	3.45883 kcal/mol	1.16529 kcal/mol	5 16@LB@ \| \|@LB@ 5'-GAUCC G AAUCC...ACU-3'@LB@ UGCGCGU UU@LB@ \|\|\|\|\|\|\| \|\|@LB@ ACGCGCA AA@LB@3'-UUU...UGUUU G AG-5'@LB@ \| \|@LB@ 13 2@LB@@LB@@LB@Energy -9.49 kcal/mol@LB@Hybridization Energy -14.1156 kcal/mol@LB@Unfolding Energy - mRNA 3.45883 kcal/mol@LB@Unfolding Energy - sRNA 1.16529 kcal/mol@LB@@LB@Position - mRNA 6 -- 15@LB@Position - sRNA 3 -- 12@LB@Position Seed - mRNA 6 -- 12@LB@Position Seed - sRNA 6 -- 12@LB@
152	0.01445	0.3104	b0385	yaiC	-12.21	0.050169	39 -- 67	12 -- 42	diguanylate cyclase cellulose regualtor		_____________38_______________________________68\n_____________\|________________________________\|\n5'-CAA...CAACA_______________U______C_________UUAUG...CCG-3'\n______________AUCUCUGAA___AGG_GAUGAU_GUAAUAAGU\n______________\|\|\|\|\|\|\|\|\|___\|\|\|_\|\|\|\|\|\|_:\|\|\|:\|\|:\|\n______________UAGAGACUU___UCC_CUACUA_UAUUGUUUA\n3'-UUU...UAAAG_________AAG__________C_________CGCGC...AAG-5'\n_____________\|________________________________\|\n_____________43_______________________________11\n	40 -- 46	35 -- 41	-28.8147 kcal/mol	7.19368 kcal/mol	9.40905 kcal/mol	38 68@LB@ \| \|@LB@5'-CAA...CAACA U C UUAUG...CCG-3'@LB@ AUCUCUGAA AGG GAUGAU GUAAUAAGU@LB@ \|\|\|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\| :\|\|\|:\|\|:\|@LB@ UAGAGACUU UCC CUACUA UAUUGUUUA@LB@3'-UUU...UAAAG AAG C CGCGC...AAG-5'@LB@ \| \|@LB@ 43 11@LB@@LB@@LB@Energy -12.21 kcal/mol@LB@Hybridization Energy -28.8147 kcal/mol@LB@Unfolding Energy - mRNA 7.19368 kcal/mol@LB@Unfolding Energy - sRNA 9.40905 kcal/mol@LB@@LB@Position - mRNA 39 -- 67@LB@Position - sRNA 12 -- 42@LB@Position Seed - mRNA 40 -- 46@LB@Position Seed - sRNA 35 -- 41@LB@
23	0.001384	0.1943	b0399	phoB	-10.33	0.106753	53 -- 63	8 -- 18	response regulator in two-component regulatory system with PhoR		_____________52__________64\n_____________\|___________\|\n5'-AGG...ACCCA___________CAUUU...AAG-3'\n______________AACAGAUGUGC\n______________\|\|\|\|:\|\|\|:\|\|\n______________UUGUUUACGCG\n3'-UUU...UACUA___________CAGAAAG-5'\n_____________\|___________\|\n_____________19__________7\n	57 -- 63	8 -- 14	-14.9736 kcal/mol	3.44457 kcal/mol	1.20363 kcal/mol	52 64@LB@ \| \|@LB@5'-AGG...ACCCA CAUUU...AAG-3'@LB@ AACAGAUGUGC@LB@ \|\|\|\|:\|\|\|:\|\|@LB@ UUGUUUACGCG@LB@3'-UUU...UACUA CAGAAAG-5'@LB@ \| \|@LB@ 19 7@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -14.9736 kcal/mol@LB@Unfolding Energy - mRNA 3.44457 kcal/mol@LB@Unfolding Energy - sRNA 1.20363 kcal/mol@LB@@LB@Position - mRNA 53 -- 63@LB@Position - sRNA 8 -- 18@LB@Position Seed - mRNA 57 -- 63@LB@Position Seed - sRNA 8 -- 14@LB@
182	0.01795	0.3104	b0403	malZ	-8.22	0.235274	180 -- 211	10 -- 46	maltodextrin glucosidase		_____________179_____________________________________212\n_____________\|_______________________________________\|\n5'-UUU...CAGAU_______A_____________AAUU______U_______AUGGC...AGC-3'\n______________UUUCAUC________CAGGGG____AUGAUG_UAAAUGC\n______________\|\|\|\|\|\|\|________\|\|\|\|\|:____\|\|\|\|\|:_:\|\|\|\|\|\|\n______________AAAGUAG________GUCCCU____UACUAU_GUUUACG\n3'-UUU...GUUUU_______AGACUUAA______AC________U_______CGCAG...AAG-5'\n_____________\|_______________________________________\|\n_____________47______________________________________9\n	205 -- 211	10 -- 16	-25.6812 kcal/mol	8.00905 kcal/mol	9.44998 kcal/mol	179 212@LB@ \| \|@LB@5'-UUU...CAGAU A AAUU U AUGGC...AGC-3'@LB@ UUUCAUC CAGGGG AUGAUG UAAAUGC@LB@ \|\|\|\|\|\|\| \|\|\|\|\|: \|\|\|\|\|: :\|\|\|\|\|\|@LB@ AAAGUAG GUCCCU UACUAU GUUUACG@LB@3'-UUU...GUUUU AGACUUAA AC U CGCAG...AAG-5'@LB@ \| \|@LB@ 47 9@LB@@LB@@LB@Energy -8.22 kcal/mol@LB@Hybridization Energy -25.6812 kcal/mol@LB@Unfolding Energy - mRNA 8.00905 kcal/mol@LB@Unfolding Energy - sRNA 9.44998 kcal/mol@LB@@LB@Position - mRNA 180 -- 211@LB@Position - sRNA 10 -- 46@LB@Position Seed - mRNA 205 -- 211@LB@Position Seed - sRNA 10 -- 16@LB@
88	0.008928	0.3104	b0405	queA	-8.89	0.184414	201 -- 209	5 -- 13	S-adenosylmethionine:tRNA ribosyltransferase-isomerase		_____________200_______210\n_____________\|_________\|\n5'-GGG...GUGUC_________ACCGA...GGC-3'\n______________AUGCGCGUU\n______________\|\|\|\|\|\|\|\|:\n______________UACGCGCAG\n3'-UUU...UUGUU_________AAAG-5'\n_____________\|_________\|\n_____________14________4\n	203 -- 209	5 -- 11	-14.1161 kcal/mol	4.07268 kcal/mol	1.15134 kcal/mol	200 210@LB@ \| \|@LB@5'-GGG...GUGUC ACCGA...GGC-3'@LB@ AUGCGCGUU@LB@ \|\|\|\|\|\|\|\|:@LB@ UACGCGCAG@LB@3'-UUU...UUGUU AAAG-5'@LB@ \| \|@LB@ 14 4@LB@@LB@@LB@Energy -8.89 kcal/mol@LB@Hybridization Energy -14.1161 kcal/mol@LB@Unfolding Energy - mRNA 4.07268 kcal/mol@LB@Unfolding Energy - sRNA 1.15134 kcal/mol@LB@@LB@Position - mRNA 201 -- 209@LB@Position - sRNA 5 -- 13@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 5 -- 11@LB@
48	0.00405	0.2857	b0409	secF	-13.37	0.031213	166 -- 172	5 -- 11	SecYEG protein translocase auxillary subunit		_____________165_____173\n_____________\|_______\|\n5'-CUU...GGCAA_______AAGAA...UGU-3'\n______________GCGCGUC\n______________\|\|\|\|\|\|\|\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	166 -- 172	5 -- 11	-14.9857 kcal/mol	0.509172 kcal/mol	1.11145 kcal/mol	165 173@LB@ \| \|@LB@5'-CUU...GGCAA AAGAA...UGU-3'@LB@ GCGCGUC@LB@ \|\|\|\|\|\|\|@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -13.37 kcal/mol@LB@Hybridization Energy -14.9857 kcal/mol@LB@Unfolding Energy - mRNA 0.509172 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 166 -- 172@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 5 -- 11@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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