Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
67	0.006163	0.3087	b0773	ybhB	-10.36	0.105479	214 -- 228	7 -- 20	kinase inhibitor homolog UPF0098 family	b0545	_____________213_____________229\n_____________\|_______________\|\n5'-UGU...CAUCA_______UC______AUGGC...AUC-3'\n______________GUAACGA__UGCGCG\n______________:\|\|\|\|:\|__\|\|\|\|\|\|\n______________UAUUGUU__ACGCGC\n3'-UUU...ACUAC_______U_______AGAAAG-5'\n_____________\|_______________\|\n_____________21______________6\n	214 -- 220	14 -- 20	-16.0436 kcal/mol	4.44842 kcal/mol	1.23906 kcal/mol	213 229@LB@ \| \|@LB@5'-UGU...CAUCA UC AUGGC...AUC-3'@LB@ GUAACGA UGCGCG@LB@ :\|\|\|\|:\| \|\|\|\|\|\|@LB@ UAUUGUU ACGCGC@LB@3'-UUU...ACUAC U AGAAAG-5'@LB@ \| \|@LB@ 21 6@LB@@LB@@LB@Energy -10.36 kcal/mol@LB@Hybridization Energy -16.0436 kcal/mol@LB@Unfolding Energy - mRNA 4.44842 kcal/mol@LB@Unfolding Energy - sRNA 1.23906 kcal/mol@LB@@LB@Position - mRNA 214 -- 228@LB@Position - sRNA 7 -- 20@LB@Position Seed - mRNA 214 -- 220@LB@Position Seed - sRNA 14 -- 20@LB@
95	0.009629	0.3104	b0781	moaA	-11.57	0.065137	257 -- 279	1 -- 21	molybdopterin biosynthesis protein A		_____________256_____________________280\n_____________\|_______________________\|\n5'-ACG...CUGUC___U__C_________AA_____GUUGC...CGG-3'\n______________GAU_AC_GAUGUGUGU__CUUUC\n______________\|\|\|_\|\|_:\|\|\|:\|:\|\|__\|\|\|\|\|\n______________CUA_UG_UUACGCGCA__GAAAG\n3'-UUU...UACUA___U__U________________-5'\n_____________\|______________________\|\n_____________22_____________________1\n	266 -- 272	6 -- 12	-18.6522 kcal/mol	5.82624 kcal/mol	1.25763 kcal/mol	256 280@LB@ \| \|@LB@5'-ACG...CUGUC U C AA GUUGC...CGG-3'@LB@ GAU AC GAUGUGUGU CUUUC@LB@ \|\|\| \|\| :\|\|\|:\|:\|\| \|\|\|\|\|@LB@ CUA UG UUACGCGCA GAAAG@LB@3'-UUU...UACUA U U -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -11.57 kcal/mol@LB@Hybridization Energy -18.6522 kcal/mol@LB@Unfolding Energy - mRNA 5.82624 kcal/mol@LB@Unfolding Energy - sRNA 1.25763 kcal/mol@LB@@LB@Position - mRNA 257 -- 279@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 266 -- 272@LB@Position Seed - sRNA 6 -- 12@LB@
8	0.000167	0.07068	b0814	ompX	-11.11	0.078421	202 -- 223	1 -- 22	outer membrane protein X		_____________201____________________224\n_____________\|______________________\|\n5'-CUG...GGUUA___AAAA__U___A________AGCAC...CAC-3'\n______________UGA____AA_UGC_UGUCUUUC\n______________\|\|\|____\|\|_\|\|\|_:\|\|\|\|\|\|\|\n______________ACU____UU_ACG_GCAGAAAG\n3'-UUU...CUACU___AUUG__U___C________-5'\n_____________\|_____________________\|\n_____________23____________________1\n	217 -- 223	1 -- 7	-15.5509 kcal/mol	3.18554 kcal/mol	1.25963 kcal/mol	201 224@LB@ \| \|@LB@5'-CUG...GGUUA AAAA U A AGCAC...CAC-3'@LB@ UGA AA UGC UGUCUUUC@LB@ \|\|\| \|\| \|\|\| :\|\|\|\|\|\|\|@LB@ ACU UU ACG GCAGAAAG@LB@3'-UUU...CUACU AUUG U C -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -11.11 kcal/mol@LB@Hybridization Energy -15.5509 kcal/mol@LB@Unfolding Energy - mRNA 3.18554 kcal/mol@LB@Unfolding Energy - sRNA 1.25963 kcal/mol@LB@@LB@Position - mRNA 202 -- 223@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 1 -- 7@LB@
77	0.007102	0.3087	b0832	gsiD	-8.78	0.192254	77 -- 85	7 -- 15	glutathione ABC transporter permease		_____________76________86\n_____________\|_________\|\n5'-UUG...CGUAG_________AUUAC...GAU-3'\n______________AAAUGCGUG\n______________\|\|\|\|\|\|\|:\|\n______________UUUACGCGC\n3'-UUU...UAUUG_________AGAAAG-5'\n_____________\|_________\|\n_____________16________6\n	79 -- 85	7 -- 13	-12.0627 kcal/mol	2.10506 kcal/mol	1.17837 kcal/mol	76 86@LB@ \| \|@LB@5'-UUG...CGUAG AUUAC...GAU-3'@LB@ AAAUGCGUG@LB@ \|\|\|\|\|\|\|:\|@LB@ UUUACGCGC@LB@3'-UUU...UAUUG AGAAAG-5'@LB@ \| \|@LB@ 16 6@LB@@LB@@LB@Energy -8.78 kcal/mol@LB@Hybridization Energy -12.0627 kcal/mol@LB@Unfolding Energy - mRNA 2.10506 kcal/mol@LB@Unfolding Energy - sRNA 1.17837 kcal/mol@LB@@LB@Position - mRNA 77 -- 85@LB@Position - sRNA 7 -- 15@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 7 -- 13@LB@
162	0.01506	0.3104	b0871	poxB	-6.02	0.481603	228 -- 255	23 -- 53	pyruvate dehydrogenase thiamine triphosphate-binding FAD-binding		_____________227_______________________________256\n_____________\|_________________________________\|\n5'-CCG...AUAUC_____________________CGG_________AACGC...GUG-3'\n______________GCCAAAA___CA_CUC_GAAU___CAGGGGUGA\n______________\|\|\|\|\|\|\|___\|\|_\|\|\|_\|\|\|\|___\|\|\|\|\|:\|\|\|\n______________CGGUUUU___GU_GAG_CUUA___GUCCCUACU\n3'-UUU...CUCAC_______AAA__A___A____A___________ACUAU...AAG-5'\n_____________\|_________________________________\|\n_____________54________________________________22\n	249 -- 255	23 -- 29	-28.0165 kcal/mol	6.84512 kcal/mol	15.1535 kcal/mol	227 256@LB@ \| \|@LB@5'-CCG...AUAUC CGG AACGC...GUG-3'@LB@ GCCAAAA CA CUC GAAU CAGGGGUGA@LB@ \|\|\|\|\|\|\| \|\| \|\|\| \|\|\|\| \|\|\|\|\|:\|\|\|@LB@ CGGUUUU GU GAG CUUA GUCCCUACU@LB@3'-UUU...CUCAC AAA A A A ACUAU...AAG-5'@LB@ \| \|@LB@ 54 22@LB@@LB@@LB@Energy -6.02 kcal/mol@LB@Hybridization Energy -28.0165 kcal/mol@LB@Unfolding Energy - mRNA 6.84512 kcal/mol@LB@Unfolding Energy - sRNA 15.1535 kcal/mol@LB@@LB@Position - mRNA 228 -- 255@LB@Position - sRNA 23 -- 53@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 23 -- 29@LB@
14	0.0004057	0.09812	b0886	cydC	-15.95	0.010534	194 -- 211	2 -- 21	glutathione/cysteine ABC transporter export permease/ATPase		_____________193__________________212\n_____________\|____________________\|\n5'-GCG...GAGGA___U________________GCUAC...UCG-3'\n______________GAU__UAAAUGCGCG_CUUU\n______________\|\|\|__:\|\|\|\|\|\|\|\|\|_\|\|\|\|\n______________CUA__GUUUACGCGC_GAAA\n3'-UUU...UACUA___UU__________A____G-5'\n_____________\|____________________\|\n_____________22___________________1\n	201 -- 207	7 -- 13	-19.6155 kcal/mol	2.41081 kcal/mol	1.25756 kcal/mol	193 212@LB@ \| \|@LB@5'-GCG...GAGGA U GCUAC...UCG-3'@LB@ GAU UAAAUGCGCG CUUU@LB@ \|\|\| :\|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ CUA GUUUACGCGC GAAA@LB@3'-UUU...UACUA UU A G-5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -15.95 kcal/mol@LB@Hybridization Energy -19.6155 kcal/mol@LB@Unfolding Energy - mRNA 2.41081 kcal/mol@LB@Unfolding Energy - sRNA 1.25756 kcal/mol@LB@@LB@Position - mRNA 194 -- 211@LB@Position - sRNA 2 -- 21@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 7 -- 13@LB@
41	0.00276	0.2279	b0889	lrp	-10.49	0.100208	207 -- 221	8 -- 21	leucine-responsive global transcriptional regulator		_____________206_____________222\n_____________\|_______________\|\n5'-GUG...UGGUA_________AA____CCUGG...AGC-3'\n______________GAUAGCAAG__GCGC\n______________\|\|\|\|:\|\|\|:__\|\|\|\|\n______________CUAUUGUUU__CGCG\n3'-UUU...UACUA_________A_____CAGAAAG-5'\n_____________\|_______________\|\n_____________22______________7\n	209 -- 215	13 -- 19	-15.2704 kcal/mol	3.5472 kcal/mol	1.23638 kcal/mol	206 222@LB@ \| \|@LB@5'-GUG...UGGUA AA CCUGG...AGC-3'@LB@ GAUAGCAAG GCGC@LB@ \|\|\|\|:\|\|\|: \|\|\|\|@LB@ CUAUUGUUU CGCG@LB@3'-UUU...UACUA A CAGAAAG-5'@LB@ \| \|@LB@ 22 7@LB@@LB@@LB@Energy -10.49 kcal/mol@LB@Hybridization Energy -15.2704 kcal/mol@LB@Unfolding Energy - mRNA 3.5472 kcal/mol@LB@Unfolding Energy - sRNA 1.23638 kcal/mol@LB@@LB@Position - mRNA 207 -- 221@LB@Position - sRNA 8 -- 21@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 13 -- 19@LB@
157	0.01467	0.3104	b0906	ycaP	-12.44	0.045773	54 -- 69	5 -- 18	UPF0702 family putative inner membrane protein		_____________53_______________70\n_____________\|________________\|\n5'-AGU...CCGGG_________UU_____AUGAA...UCU-3'\n______________GAUAAAUGU__GCGUC\n______________:\|:\|\|\|\|\|:__\|\|\|\|\|\n______________UUGUUUACG__CGCAG\n3'-UUU...UACUA________________AAAG-5'\n_____________\|________________\|\n_____________19_______________4\n	56 -- 62	10 -- 16	-16.0437 kcal/mol	2.38303 kcal/mol	1.2243 kcal/mol	53 70@LB@ \| \|@LB@5'-AGU...CCGGG UU AUGAA...UCU-3'@LB@ GAUAAAUGU GCGUC@LB@ :\|:\|\|\|\|\|: \|\|\|\|\|@LB@ UUGUUUACG CGCAG@LB@3'-UUU...UACUA AAAG-5'@LB@ \| \|@LB@ 19 4@LB@@LB@@LB@Energy -12.44 kcal/mol@LB@Hybridization Energy -16.0437 kcal/mol@LB@Unfolding Energy - mRNA 2.38303 kcal/mol@LB@Unfolding Energy - sRNA 1.2243 kcal/mol@LB@@LB@Position - mRNA 54 -- 69@LB@Position - sRNA 5 -- 18@LB@Position Seed - mRNA 56 -- 62@LB@Position Seed - sRNA 10 -- 16@LB@
85	0.008151	0.3104	b0914	msbA	-12.09	0.052799	181 -- 198	1 -- 18	lipid ABC transporter permease/ATPase		_____________180________________199\n_____________\|__________________\|\n5'-AGC...AUUUC_________UG_______GAAUG...CGU-3'\n______________AACAAAUGC__GUUUUUU\n______________\|\|\|\|\|\|\|\|\|__\|\|:\|\|\|:\n______________UUGUUUACG__CAGAAAG\n3'-UUU...UACUA_________CG_______-5'\n_____________\|_________________\|\n_____________19________________1\n	192 -- 198	1 -- 7	-16.7201 kcal/mol	3.39277 kcal/mol	1.24077 kcal/mol	180 199@LB@ \| \|@LB@5'-AGC...AUUUC UG GAAUG...CGU-3'@LB@ AACAAAUGC GUUUUUU@LB@ \|\|\|\|\|\|\|\|\| \|\|:\|\|\|:@LB@ UUGUUUACG CAGAAAG@LB@3'-UUU...UACUA CG -5'@LB@ \| \|@LB@ 19 1@LB@@LB@@LB@Energy -12.09 kcal/mol@LB@Hybridization Energy -16.7201 kcal/mol@LB@Unfolding Energy - mRNA 3.39277 kcal/mol@LB@Unfolding Energy - sRNA 1.24077 kcal/mol@LB@@LB@Position - mRNA 181 -- 198@LB@Position - sRNA 1 -- 18@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 1 -- 7@LB@
120	0.01222	0.3104	b0916	ycaQ	-9.32	0.156865	225 -- 243	1 -- 17	DUF1006 family protein with C-terminal wHTH domain		_____________224_________________244\n_____________\|___________________\|\n5'-UGG...CCCUU__U_________AAUC___ACCUU...AUA-3'\n______________GC_GAUGCGCGU____UUC\n______________:\|_:\|\|\|\|\|\|\|\|____\|\|\|\n______________UG_UUACGCGCA____AAG\n3'-UUU...ACUAU__U_________GA_____-5'\n_____________\|__________________\|\n_____________18_________________1\n	230 -- 236	6 -- 12	-16.5738 kcal/mol	6.00902 kcal/mol	1.23994 kcal/mol	224 244@LB@ \| \|@LB@5'-UGG...CCCUU U AAUC ACCUU...AUA-3'@LB@ GC GAUGCGCGU UUC@LB@ :\| :\|\|\|\|\|\|\|\| \|\|\|@LB@ UG UUACGCGCA AAG@LB@3'-UUU...ACUAU U GA -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -9.32 kcal/mol@LB@Hybridization Energy -16.5738 kcal/mol@LB@Unfolding Energy - mRNA 6.00902 kcal/mol@LB@Unfolding Energy - sRNA 1.23994 kcal/mol@LB@@LB@Position - mRNA 225 -- 243@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 6 -- 12@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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