Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA
10	0.002311	0.4469	lmo2106	N/A	-20.89	0.006356	172 -- 222	159 -- 207	_____________171__________________________________________________223\n_____________\|____________________________________________________\|\n5'-AGU...UAAUA_______AC__CAA______G_____AAA____U__UUAA_______G____UUAUA...AUG-3'\n______________AAUGGGA__AG___CAUAGA_GUGAA____AUG_CU____AGAUGAA_UGGA\n______________\|\|:\|\|\|\|__\|\|___\|\|\|\|\|\|_:\|\|\|\|____\|:\|_\|:____\|\|\|\|\|\|\|_\|\|:\|\n______________UUGCCCU__UC___GUAUCU_UACUU____UGC_GG____UCUACUU_ACUU\n3'-GUU...ACGUA_______AC__C________G_____GAUG___C__UUAC____________UUGUU...GGA-5'\n_____________\|____________________________________________________\|\n_____________208__________________________________________________158\n	211 -- 217	163 -- 169	-34.9864 kcal/mol	6.68059 kcal/mol	18.5724 kcal/mol	171 223@LB@ \| \|@LB@5'-AGU...UAAUA AC CAA G AAA U UUAA G UUAUA...AUG-3'@LB@ AAUGGGA AG CAUAGA GUGAA AUG CU AGAUGAA UGGA@LB@ \|\|:\|\|\|\| \|\| \|\|\|\|\|\| :\|\|\|\| \|:\| \|: \|\|\|\|\|\|\| \|\|:\|@LB@ UUGCCCU UC GUAUCU UACUU UGC GG UCUACUU ACUU@LB@3'-GUU...ACGUA AC C G GAUG C UUAC UUGUU...GGA-5'@LB@ \| \|@LB@ 208 158@LB@@LB@@LB@Energy -20.89 kcal/mol@LB@Hybridization Energy -34.9864 kcal/mol@LB@Unfolding Energy - mRNA 6.68059 kcal/mol@LB@Unfolding Energy - sRNA 18.5724 kcal/mol@LB@@LB@Position - mRNA 172 -- 222@LB@Position - sRNA 159 -- 207@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 163 -- 169@LB@
19	0.003536	0.4469	lmo0321	N/A	-22.74	0.002408	114 -- 157	167 -- 207	_____________113____________________________________________158\n_____________\|______________________________________________\|\n5'-AUU...UGUUA________UA____G_AC__________GGA_____U_________AUGUU...AGG-3'\n______________AGCGGGAU__GAGG_A__UAGGUA_GAA____ACGG_CUAAUGAGA\n______________\|:\|\|\|\|\|\|__\|\|\|\|_\|__\|\|\|::\|_\|\|\|____\|\|\|\|_\|:\|\|\|\|\|\|\|\n______________UUGCCCUA__CUCC_U__AUCUGU_CUU____UGCC_GGUUACUCU\n3'-GUU...ACGUA______________G_________A___GAUG______________ACUUA...GGA-5'\n_____________\|______________________________________________\|\n_____________208____________________________________________166\n	151 -- 157	167 -- 173	-39.7249 kcal/mol	9.73371 kcal/mol	18.0488 kcal/mol	113 158@LB@ \| \|@LB@5'-AUU...UGUUA UA G AC GGA U AUGUU...AGG-3'@LB@ AGCGGGAU GAGG A UAGGUA GAA ACGG CUAAUGAGA@LB@ \|:\|\|\|\|\|\| \|\|\|\| \| \|\|\|::\| \|\|\| \|\|\|\| \|:\|\|\|\|\|\|\|@LB@ UUGCCCUA CUCC U AUCUGU CUU UGCC GGUUACUCU@LB@3'-GUU...ACGUA G A GAUG ACUUA...GGA-5'@LB@ \| \|@LB@ 208 166@LB@@LB@@LB@Energy -22.74 kcal/mol@LB@Hybridization Energy -39.7249 kcal/mol@LB@Unfolding Energy - mRNA 9.73371 kcal/mol@LB@Unfolding Energy - sRNA 18.0488 kcal/mol@LB@@LB@Position - mRNA 114 -- 157@LB@Position - sRNA 167 -- 207@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 167 -- 173@LB@
25	0.004689	0.5029	lmo1584	N/A	-20.33	0.008463	183 -- 211	169 -- 209	_____________182___________212\n_____________\|_____________\|\n5'-GAA...GGCGC_____________AAAUG...CUG-3'\n______________ACAUGGCUAAUGA\n______________\|\|\|:\|\|\|:\|\|\|\|\|\n______________UGUGCCGGUUACU\n3'-GUU...CUUGA_____________CUACU...GGA-5'\n_____________\|_____________\|\n_____________210___________168\n	205 -- 211	169 -- 175	-19.4521 kcal/mol	2.17631 kcal/mol	6.15322 kcal/mol	182 212@LB@ \| \|@LB@5'-GAA...GGCGC AAAUG...CUG-3'@LB@ ACAUGGCUAAUGA@LB@ \|\|\|:\|\|\|:\|\|\|\|\|@LB@ UGUGCCGGUUACU@LB@3'-GUU...CUUGA CUACU...GGA-5'@LB@ \| \|@LB@ 210 168@LB@@LB@@LB@Energy -20.33 kcal/mol@LB@Hybridization Energy -19.4521 kcal/mol@LB@Unfolding Energy - mRNA 2.17631 kcal/mol@LB@Unfolding Energy - sRNA 6.15322 kcal/mol@LB@@LB@Position - mRNA 183 -- 211@LB@Position - sRNA 169 -- 209@LB@Position Seed - mRNA 205 -- 211@LB@Position Seed - sRNA 169 -- 175@LB@
31	0.00657	0.5515	lmo1828	N/A	-18.66	0.019040	211 -- 279	154 -- 211	_____________210__________280\n_____________\|____________\|\n5'-AGU...GAAAA____________UUGGG...AAU-3'\n______________GCAUGACGGGAU\n______________\|\|\|\|:\|\|\|\|\|\|\|\n______________CGUAUUGCCCUA\n3'-GUU...GGAUA____________CUCCG...GGA-5'\n_____________\|____________\|\n_____________212__________153\n	216 -- 222	200 -- 206	-19.8213 kcal/mol	2.93239 kcal/mol	6.43166 kcal/mol	210 280@LB@ \| \|@LB@5'-AGU...GAAAA UUGGG...AAU-3'@LB@ GCAUGACGGGAU@LB@ \|\|\|\|:\|\|\|\|\|\|\|@LB@ CGUAUUGCCCUA@LB@3'-GUU...GGAUA CUCCG...GGA-5'@LB@ \| \|@LB@ 212 153@LB@@LB@@LB@Energy -18.66 kcal/mol@LB@Hybridization Energy -19.8213 kcal/mol@LB@Unfolding Energy - mRNA 2.93239 kcal/mol@LB@Unfolding Energy - sRNA 6.43166 kcal/mol@LB@@LB@Position - mRNA 211 -- 279@LB@Position - sRNA 154 -- 211@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 200 -- 206@LB@
33	0.006763	0.5515	lmo1941	N/A	-21.42	0.004845	223 -- 286	154 -- 207	_____________222___________________________________________________________________287\n_____________\|_____________________________________________________________________\|\n5'-UAG...AGAAA_______AUACGAAGAACC___UGAUUAU________UU___AA_________________UC______UGAAC...UGU-3'\n______________AACGGGA____________GGC_______GACAUGAG__CUG___GGCCA___AGAUGAAU___AACAA\n______________\|\|\|\|\|\|\|____________\|\|\|_______\|\|\|\|\|\|\|:__\|\|:___\|\|\|\|\|___\|\|\|\|\|\|\|\|___\|\|\|\|\|\n______________UUGCCCU____________CCG_______CUGUACUU__GAU___CCGGU___UCUACUUA___UUGUU\n3'-GUU...ACGUA_______ACU____________UAU_________________GUG_____UAC________CUU_____UAUUA...GGA-5'\n_____________\|_____________________________________________________________________\|\n_____________208___________________________________________________________________153\n	273 -- 279	162 -- 168	-39.5283 kcal/mol	10.6608 kcal/mol	18.6655 kcal/mol	222 287@LB@ \| \|@LB@5'-UAG...AGAAA AUACGAAGAACC UGAUUAU UU AA UC UGAAC...UGU-3'@LB@ AACGGGA GGC GACAUGAG CUG GGCCA AGAUGAAU AACAA@LB@ \|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|: \|\|: \|\|\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|@LB@ UUGCCCU CCG CUGUACUU GAU CCGGU UCUACUUA UUGUU@LB@3'-GUU...ACGUA ACU UAU GUG UAC CUU UAUUA...GGA-5'@LB@ \| \|@LB@ 208 153@LB@@LB@@LB@Energy -21.42 kcal/mol@LB@Hybridization Energy -39.5283 kcal/mol@LB@Unfolding Energy - mRNA 10.6608 kcal/mol@LB@Unfolding Energy - sRNA 18.6655 kcal/mol@LB@@LB@Position - mRNA 223 -- 286@LB@Position - sRNA 154 -- 207@LB@Position Seed - mRNA 273 -- 279@LB@Position Seed - sRNA 162 -- 168@LB@
35	0.00718	0.552	lmo0397	N/A	-24.49	0.000911	178 -- 232	160 -- 211	_____________177_________________________________________________________________________233\n_____________\|___________________________________________________________________________\|\n5'-AAU...ACAAA_______AAAAUGAAAGCAAAU_____________G__UU_G______GAG___AU__AA___C___________UAUGA...CGA-3'\n______________CUGUGCG_______________UGGCGGGA___GG_AU__A_AUGAAC___GCA__CG__CGA_GAGAUGA_UGA\n______________\|\|:\|\|\|:_______________\|::\|\|\|\|\|___\|\|_\|\|__\|_\|\|\|\|\|\|___:\|\|__\|\|__\|:\|_\|\|\|\|\|\|\|_\|\|\|\n______________GAUACGU_______________AUUGCCCU___CC_UA__U_UACUUG___UGU__GC__GUU_CUCUACU_ACU\n3'-GUU...CCGCG______________________________ACU__G__UC_G______A_________CG___A_______U___UUUGU...GGA-5'\n_____________\|___________________________________________________________________________\|\n_____________212_________________________________________________________________________159\n	223 -- 229	164 -- 170	-45.3132 kcal/mol	12.0365 kcal/mol	19.7814 kcal/mol	177 233@LB@ \| \|@LB@5'-AAU...ACAAA AAAAUGAAAGCAAAU G UU G GAG AU AA C UAUGA...CGA-3'@LB@ CUGUGCG UGGCGGGA GG AU A AUGAAC GCA CG CGA GAGAUGA UGA@LB@ \|\|:\|\|\|: \|::\|\|\|\|\| \|\| \|\| \| \|\|\|\|\|\| :\|\| \|\| \|:\| \|\|\|\|\|\|\| \|\|\|@LB@ GAUACGU AUUGCCCU CC UA U UACUUG UGU GC GUU CUCUACU ACU@LB@3'-GUU...CCGCG ACU G UC G A CG A U UUUGU...GGA-5'@LB@ \| \|@LB@ 212 159@LB@@LB@@LB@Energy -24.49 kcal/mol@LB@Hybridization Energy -45.3132 kcal/mol@LB@Unfolding Energy - mRNA 12.0365 kcal/mol@LB@Unfolding Energy - sRNA 19.7814 kcal/mol@LB@@LB@Position - mRNA 178 -- 232@LB@Position - sRNA 160 -- 211@LB@Position Seed - mRNA 223 -- 229@LB@Position Seed - sRNA 164 -- 170@LB@
38	0.007855	0.5562	lmo1229	N/A	-18.27	0.022927	193 -- 228	147 -- 186	_____________192_______________________________________229\n_____________\|_________________________________________\|\n5'-AUU...AGGAG___U_________A________________UA_________CAACA...GCG-3'\n______________GAA_UUACGUGGC_AAUGAGA_____GAAA__AAGUAGUGA\n______________\|\|\|_:\|\|\|::\|\|\|_\|\|\|\|\|\|\|_____\|\|\|\|__\|\|:\|\|:\|\|\|\n______________CUU_GAUGUGCCG_UUACUCU_____CUUU__UUUAUUACU\n3'-GUU...CUGUA_____________G_______ACUUA____UG_________AAAAC...GGA-5'\n_____________\|_________________________________________\|\n_____________187_______________________________________146\n	207 -- 213	167 -- 173	-29.0601 kcal/mol	3.84241 kcal/mol	14.4129 kcal/mol	192 229@LB@ \| \|@LB@5'-AUU...AGGAG U A UA CAACA...GCG-3'@LB@ GAA UUACGUGGC AAUGAGA GAAA AAGUAGUGA@LB@ \|\|\| :\|\|\|::\|\|\| \|\|\|\|\|\|\| \|\|\|\| \|\|:\|\|:\|\|\|@LB@ CUU GAUGUGCCG UUACUCU CUUU UUUAUUACU@LB@3'-GUU...CUGUA G ACUUA UG AAAAC...GGA-5'@LB@ \| \|@LB@ 187 146@LB@@LB@@LB@Energy -18.27 kcal/mol@LB@Hybridization Energy -29.0601 kcal/mol@LB@Unfolding Energy - mRNA 3.84241 kcal/mol@LB@Unfolding Energy - sRNA 14.4129 kcal/mol@LB@@LB@Position - mRNA 193 -- 228@LB@Position - sRNA 147 -- 186@LB@Position Seed - mRNA 207 -- 213@LB@Position Seed - sRNA 167 -- 173@LB@
40	0.00848	0.5705	lmo2399	N/A	-21.52	0.004592	127 -- 195	156 -- 207	_____________126_________________________________________________________________196\n_____________\|___________________________________________________________________\|\n5'-AUU...UCGAC_________GU_______CUUGUGGUUUAAAC____AGA____G__UAGA______A___G______GCUAA...CUG-3'\n______________GGUGAGGCA__GGCG_GA______________GCUA___ACGG_UA____AUGGGG_GAA_GAGGAU\n______________\|:\|\|\|\|\|\|\|__\|:\|:_\|\|______________:\|\|\|___\|\|\|\|_:\|____\|\|\|:\|:_\|\|\|_\|\|::\|:\n______________CUACUCCGU__CUGU_CU______________UGAU___UGCC_GU____UACUCU_CUU_CUUUUG\n3'-GUU...UUGCC_________AU____A____________________G______G____________A___A______UUUAU...GGA-5'\n_____________\|___________________________________________________________________\|\n_____________208_________________________________________________________________155\n	132 -- 138	194 -- 200	-37.8581 kcal/mol	11.6457 kcal/mol	14.014 kcal/mol	126 196@LB@ \| \|@LB@5'-AUU...UCGAC GU CUUGUGGUUUAAAC AGA G UAGA A G GCUAA...CUG-3'@LB@ GGUGAGGCA GGCG GA GCUA ACGG UA AUGGGG GAA GAGGAU@LB@ \|:\|\|\|\|\|\|\| \|:\|: \|\| :\|\|\| \|\|\|\| :\| \|\|\|:\|: \|\|\| \|\|::\|:@LB@ CUACUCCGU CUGU CU UGAU UGCC GU UACUCU CUU CUUUUG@LB@3'-GUU...UUGCC AU A G G A A UUUAU...GGA-5'@LB@ \| \|@LB@ 208 155@LB@@LB@@LB@Energy -21.52 kcal/mol@LB@Hybridization Energy -37.8581 kcal/mol@LB@Unfolding Energy - mRNA 11.6457 kcal/mol@LB@Unfolding Energy - sRNA 14.014 kcal/mol@LB@@LB@Position - mRNA 127 -- 195@LB@Position - sRNA 156 -- 207@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 194 -- 200@LB@
50	0.01167	0.599	lmo0850	N/A	-18.65	0.019190	166 -- 190	141 -- 165	_____________165________________________191\n_____________\|__________________________\|\n5'-UGG...AAAGA____AAAAUAAAAA____________AUUUU...AAU-3'\n______________GAGA__________GAAUGAAAACAA\n______________\|\|\|\|__________\|\|\|\|\|\|\|\|\|\|\|\|\n______________CUCU__________CUUACUUUUGUU\n3'-GUU...GGUUA____A_____________________UAUUA...GGA-5'\n_____________\|__________________________\|\n_____________166________________________140\n	171 -- 177	154 -- 160	-14.3235 kcal/mol	1.0644 kcal/mol	0.933796 kcal/mol	165 191@LB@ \| \|@LB@5'-UGG...AAAGA AAAAUAAAAA AUUUU...AAU-3'@LB@ GAGA GAAUGAAAACAA@LB@ \|\|\|\| \|\|\|\|\|\|\|\|\|\|\|\|@LB@ CUCU CUUACUUUUGUU@LB@3'-GUU...GGUUA A UAUUA...GGA-5'@LB@ \| \|@LB@ 166 140@LB@@LB@@LB@Energy -18.65 kcal/mol@LB@Hybridization Energy -14.3235 kcal/mol@LB@Unfolding Energy - mRNA 1.0644 kcal/mol@LB@Unfolding Energy - sRNA 0.933796 kcal/mol@LB@@LB@Position - mRNA 166 -- 190@LB@Position - sRNA 141 -- 165@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 154 -- 160@LB@
53	0.0118	0.599	lmo2394	N/A	-15.56	0.076801	20 -- 80	100 -- 170	_____________19_____________________________________81\n_____________\|______________________________________\|\n5'-GGA...UUUUC________GUG___GG____________A_________AUAGU...AAA-3'\n______________GAUAGAUG___GAU________AUUUUG_UGGAGGAUG\n______________:\|:\|\|\|\|\|___\|\|:________\|\|\|:\|:_\|\|\|\|:\|\|\|\|\n______________UUGUCUAC___CUG________UAAGAU_ACCUUCUAC\n3'-GUU...CAGAU________AGA___AUCAACGG______A_________CUAUG...GGA-5'\n_____________\|______________________________________\|\n_____________171____________________________________99\n	189 -- 195	97 -- 103	-26.5698 kcal/mol	4.81204 kcal/mol	9.44565 kcal/mol	19 81@LB@ \| \|@LB@5'-GGA...UUUUC GUG GG A AUAGU...AAA-3'@LB@ GAUAGAUG GAU AUUUUG UGGAGGAUG@LB@ :\|:\|\|\|\|\| \|\|: \|\|\|:\|: \|\|\|\|:\|\|\|\|@LB@ UUGUCUAC CUG UAAGAU ACCUUCUAC@LB@3'-GUU...CAGAU AGA AUCAACGG A CUAUG...GGA-5'@LB@ \| \|@LB@ 171 99@LB@@LB@@LB@Energy -15.56 kcal/mol@LB@Hybridization Energy -26.5698 kcal/mol@LB@Unfolding Energy - mRNA 4.81204 kcal/mol@LB@Unfolding Energy - sRNA 9.44565 kcal/mol@LB@@LB@Position - mRNA 20 -- 80@LB@Position - sRNA 100 -- 170@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 97 -- 103@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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