Input and runtime details for job 8257533 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 8257533 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:34:31 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:34:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:30:38 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:30:45 CET 2018
	Job Completed		@ Fri Feb 16 22:31:01 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=8257533&toolName=CopraRNA ( 30 days expiry )

Description of the job

LhrA2

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003210 Listeria monocytogenes EGD-e

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
170	0.04745	0.7511	lmo2203	N/A	-15.31	0.085499	133 -- 190	99 -- 165	N-acetylmuramoyl-L-alanine amidase		_____________132___________________________________191\n_____________\|_____________________________________\|\n5'-AUA...AGGUU_________________A________AA_________ACUAA...CAG-3'\n______________AACAGA_____GAC_AG__GCCAUUU__AUUGGGGGA\n______________\|\|\|\|\|\|_____\|\|\|_\|\|__\|\|\|\|\|\|:__\|\|\|\|\|::\|\|\n______________UUGUCU_____CUG_UC__CGGUAAG__UAACCUUCU\n3'-GUU...CAGAU______ACAGA___A__AA_______A__________ACCUA...GGA-5'\n_____________\|_____________________________________\|\n_____________166___________________________________98\n	184 -- 190	99 -- 105	-25.8209 kcal/mol	4.57897 kcal/mol	9.42764 kcal/mol	132 191@LB@ \| \|@LB@5'-AUA...AGGUU A AA ACUAA...CAG-3'@LB@ AACAGA GAC AG GCCAUUU AUUGGGGGA@LB@ \|\|\|\|\|\| \|\|\| \|\| \|\|\|\|\|\|: \|\|\|\|\|::\|\|@LB@ UUGUCU CUG UC CGGUAAG UAACCUUCU@LB@3'-GUU...CAGAU ACAGA A AA A ACCUA...GGA-5'@LB@ \| \|@LB@ 166 98@LB@@LB@@LB@Energy -15.31 kcal/mol@LB@Hybridization Energy -25.8209 kcal/mol@LB@Unfolding Energy - mRNA 4.57897 kcal/mol@LB@Unfolding Energy - sRNA 9.42764 kcal/mol@LB@@LB@Position - mRNA 133 -- 190@LB@Position - sRNA 99 -- 165@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 99 -- 105@LB@
77	0.02006	0.6733	lmo1400	N/A	-17.16	0.038248	136 -- 215	157 -- 223	N-acetyltransferase		_____________135_____________216\n_____________\|_______________\|\n5'-AAA...GUUUU_______________AUUUU...AAG-3'\n______________UGAG_UGAGUGGAAA\n______________\|\|\|\|_\|\|\|:\|\|:\|\|\|\n______________ACUC_ACUUACUUUU\n3'-GUU...CGGUU____U__________GUUUA...GGA-5'\n_____________\|_______________\|\n_____________224_____________156\n	209 -- 215	157 -- 163	-14.2437 kcal/mol	2.95971 kcal/mol	0.905109 kcal/mol	135 216@LB@ \| \|@LB@5'-AAA...GUUUU AUUUU...AAG-3'@LB@ UGAG UGAGUGGAAA@LB@ \|\|\|\| \|\|\|:\|\|:\|\|\|@LB@ ACUC ACUUACUUUU@LB@3'-GUU...CGGUU U GUUUA...GGA-5'@LB@ \| \|@LB@ 224 156@LB@@LB@@LB@Energy -17.16 kcal/mol@LB@Hybridization Energy -14.2437 kcal/mol@LB@Unfolding Energy - mRNA 2.95971 kcal/mol@LB@Unfolding Energy - sRNA 0.905109 kcal/mol@LB@@LB@Position - mRNA 136 -- 215@LB@Position - sRNA 157 -- 223@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 157 -- 163@LB@
48	0.01096	0.599	lmo0184	N/A	-18.00	0.026009	159 -- 211	157 -- 203	oligo-1 6-glucosidase		_____________158_____212\n_____________\|_______\|\n5'-ACG...ACAAG_______AUUUA...GAA-3'\n______________GGCGGGA\n______________::\|\|\|\|\|\n______________UUGCCCU\n3'-GUU...ACGUA_______ACUCC...GGA-5'\n_____________\|_______\|\n_____________204_____156\n	185 -- 191	201 -- 207	-10.9952 kcal/mol	1.94403 kcal/mol	0.326294 kcal/mol	158 212@LB@ \| \|@LB@5'-ACG...ACAAG AUUUA...GAA-3'@LB@ GGCGGGA@LB@ ::\|\|\|\|\|@LB@ UUGCCCU@LB@3'-GUU...ACGUA ACUCC...GGA-5'@LB@ \| \|@LB@ 204 156@LB@@LB@@LB@Energy -18.00 kcal/mol@LB@Hybridization Energy -10.9952 kcal/mol@LB@Unfolding Energy - mRNA 1.94403 kcal/mol@LB@Unfolding Energy - sRNA 0.326294 kcal/mol@LB@@LB@Position - mRNA 159 -- 211@LB@Position - sRNA 157 -- 203@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 201 -- 207@LB@
9	0.002066	0.4469	lmo0669	N/A	-20.91	0.006284	33 -- 77	156 -- 211	oxidoreductase		_____________32__________________78\n_____________\|___________________\|\n5'-AAU...UAAAG___________________GGGAA...UUC-3'\n______________UAAUGGGG_GGAUGAAAAU\n______________:\|\|\|\|:\|:_\|:\|\|\|\|\|\|\|:\n______________GUUACUCU_CUUACUUUUG\n3'-GUU...UGCCG________A__________UUUAU...GGA-5'\n_____________\|___________________\|\n_____________212_________________155\n	71 -- 77	156 -- 162	-16.6504 kcal/mol	2.4241 kcal/mol	0.944687 kcal/mol	32 78@LB@ \| \|@LB@5'-AAU...UAAAG GGGAA...UUC-3'@LB@ UAAUGGGG GGAUGAAAAU@LB@ :\|\|\|\|:\|: \|:\|\|\|\|\|\|\|:@LB@ GUUACUCU CUUACUUUUG@LB@3'-GUU...UGCCG A UUUAU...GGA-5'@LB@ \| \|@LB@ 212 155@LB@@LB@@LB@Energy -20.91 kcal/mol@LB@Hybridization Energy -16.6504 kcal/mol@LB@Unfolding Energy - mRNA 2.4241 kcal/mol@LB@Unfolding Energy - sRNA 0.944687 kcal/mol@LB@@LB@Position - mRNA 33 -- 77@LB@Position - sRNA 156 -- 211@LB@Position Seed - mRNA 71 -- 77@LB@Position Seed - sRNA 156 -- 162@LB@
166	0.04633	0.7403	lmo1152	N/A	-16.33	0.055198	97 -- 188	95 -- 169	PduB protein		_____________96_______________189\n_____________\|________________\|\n5'-GGC...AAGUC________________AAUAA...AAG-3'\n______________AGAUGAGUGA__GCAA\n______________\|\|\|\|\|\|:\|\|\|__:\|\|\|\n______________UCUACUUACU__UGUU\n3'-GUU...GUUAC__________UU____UAUUA...GGA-5'\n_____________\|________________\|\n_____________170______________94\n	100 -- 106	160 -- 166	-13.7881 kcal/mol	2.27776 kcal/mol	0.924402 kcal/mol	96 189@LB@ \| \|@LB@5'-GGC...AAGUC AAUAA...AAG-3'@LB@ AGAUGAGUGA GCAA@LB@ \|\|\|\|\|\|:\|\|\| :\|\|\|@LB@ UCUACUUACU UGUU@LB@3'-GUU...GUUAC UU UAUUA...GGA-5'@LB@ \| \|@LB@ 170 94@LB@@LB@@LB@Energy -16.33 kcal/mol@LB@Hybridization Energy -13.7881 kcal/mol@LB@Unfolding Energy - mRNA 2.27776 kcal/mol@LB@Unfolding Energy - sRNA 0.924402 kcal/mol@LB@@LB@Position - mRNA 97 -- 188@LB@Position - sRNA 95 -- 169@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 160 -- 166@LB@
173	0.04847	0.7539	lmo1143	N/A	-15.38	0.082828	238 -- 287	171 -- 211	PduT protein		_____________237_______________________________288\n_____________\|_________________________________\|\n5'-GUG...GCAUC______GUC____C__U____AAA_________UCGGA...UUU-3'\n______________GAGGCG___GAUA_GA_GCUA___GCGGCCAAU\n______________\|\|\|\|\|:___\|\|:\|_\|\|_:\|\|\|___:\|\|\|\|\|\|\|\|\n______________CUCCGU___CUGU_CU_UGAU___UGCCGGUUA\n3'-GUU...CCCUA______AU_____A_______G___________CUCUA...GGA-5'\n_____________\|_________________________________\|\n_____________212_______________________________170\n	281 -- 287	171 -- 177	-30.6178 kcal/mol	10.0561 kcal/mol	13.341 kcal/mol	237 288@LB@ \| \|@LB@5'-GUG...GCAUC GUC C U AAA UCGGA...UUU-3'@LB@ GAGGCG GAUA GA GCUA GCGGCCAAU@LB@ \|\|\|\|\|: \|\|:\| \|\| :\|\|\| :\|\|\|\|\|\|\|\|@LB@ CUCCGU CUGU CU UGAU UGCCGGUUA@LB@3'-GUU...CCCUA AU A G CUCUA...GGA-5'@LB@ \| \|@LB@ 212 170@LB@@LB@@LB@Energy -15.38 kcal/mol@LB@Hybridization Energy -30.6178 kcal/mol@LB@Unfolding Energy - mRNA 10.0561 kcal/mol@LB@Unfolding Energy - sRNA 13.341 kcal/mol@LB@@LB@Position - mRNA 238 -- 287@LB@Position - sRNA 171 -- 211@LB@Position Seed - mRNA 281 -- 287@LB@Position Seed - sRNA 171 -- 177@LB@
60	0.01449	0.6412	lmo0835	N/A	-17.67	0.030280	187 -- 229	146 -- 187	peptidoglycan binding protein		_____________186______________________________________________230\n_____________\|________________________________________________\|\n5'-UUU...UAUGA_____UUGGAGGA____________UAAA____U____A_________UAACU...CAG-3'\n______________GAACU________AUGGUCAGUGAG____AAUG_AAAA_AAAUAGUGA\n______________\|\|\|\|\|________\|:\|\|:\|\|:\|\|\|\|____\|\|\|\|_\|\|\|\|_\|\|\|\|\|:\|\|\|\n______________CUUGA________UGCCGGUUACUC____UUAC_UUUU_UUUAUUACU\n3'-GUU...CUGUA_____UG__________________UAC__________G_________AAAAC...GGA-5'\n_____________\|________________________________________________\|\n_____________188______________________________________________145\n	199 -- 205	168 -- 174	-31.6389 kcal/mol	7.07372 kcal/mol	14.4129 kcal/mol	186 230@LB@ \| \|@LB@5'-UUU...UAUGA UUGGAGGA UAAA U A UAACU...CAG-3'@LB@ GAACU AUGGUCAGUGAG AAUG AAAA AAAUAGUGA@LB@ \|\|\|\|\| \|:\|\|:\|\|:\|\|\|\| \|\|\|\| \|\|\|\| \|\|\|\|\|:\|\|\|@LB@ CUUGA UGCCGGUUACUC UUAC UUUU UUUAUUACU@LB@3'-GUU...CUGUA UG UAC G AAAAC...GGA-5'@LB@ \| \|@LB@ 188 145@LB@@LB@@LB@Energy -17.67 kcal/mol@LB@Hybridization Energy -31.6389 kcal/mol@LB@Unfolding Energy - mRNA 7.07372 kcal/mol@LB@Unfolding Energy - sRNA 14.4129 kcal/mol@LB@@LB@Position - mRNA 187 -- 229@LB@Position - sRNA 146 -- 187@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 168 -- 174@LB@
102	0.02619	0.6733	lmo1799	N/A	-17.02	0.040600	38 -- 69	171 -- 207	peptidoglycan binding protein		_____________37______________70\n_____________\|_______________\|\n5'-CUA...AAAAC__G____________UUGGC...UGG-3'\n______________GA_GGGA_GGGGCAU\n______________:\|_\|\|\|\|_\|:\|\|\|\|\|\n______________UU_CCCU_CUCCGUA\n3'-GUU...ACGUA__G____A_______UCUGU...GGA-5'\n_____________\|_______________\|\n_____________208_____________170\n	45 -- 51	193 -- 199	-19.1902 kcal/mol	1.80232 kcal/mol	8.95053 kcal/mol	37 70@LB@ \| \|@LB@5'-CUA...AAAAC G UUGGC...UGG-3'@LB@ GA GGGA GGGGCAU@LB@ :\| \|\|\|\| \|:\|\|\|\|\|@LB@ UU CCCU CUCCGUA@LB@3'-GUU...ACGUA G A UCUGU...GGA-5'@LB@ \| \|@LB@ 208 170@LB@@LB@@LB@Energy -17.02 kcal/mol@LB@Hybridization Energy -19.1902 kcal/mol@LB@Unfolding Energy - mRNA 1.80232 kcal/mol@LB@Unfolding Energy - sRNA 8.95053 kcal/mol@LB@@LB@Position - mRNA 38 -- 69@LB@Position - sRNA 171 -- 207@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 193 -- 199@LB@
2	0.0002774	0.3688	lmo1666	N/A	-21.94	0.003684	151 -- 207	157 -- 207	peptidoglycan-linked protein	lmo0333	_____________150____________208\n_____________\|______________\|\n5'-GUG...AGAUG______________GUAAA...CUC-3'\n______________GGGAUGAAUGAAAA\n______________\|:\|\|\|\|\|\|\|\|\|\|\|\|\n______________CUCUACUUACUUUU\n3'-GUU...GGUUA______________GUUUA...GGA-5'\n_____________\|______________\|\n_____________208____________156\n	201 -- 207	157 -- 163	-18.267 kcal/mol	1.529 kcal/mol	0.904482 kcal/mol	150 208@LB@ \| \|@LB@5'-GUG...AGAUG GUAAA...CUC-3'@LB@ GGGAUGAAUGAAAA@LB@ \|:\|\|\|\|\|\|\|\|\|\|\|\|@LB@ CUCUACUUACUUUU@LB@3'-GUU...GGUUA GUUUA...GGA-5'@LB@ \| \|@LB@ 208 156@LB@@LB@@LB@Energy -21.94 kcal/mol@LB@Hybridization Energy -18.267 kcal/mol@LB@Unfolding Energy - mRNA 1.529 kcal/mol@LB@Unfolding Energy - sRNA 0.904482 kcal/mol@LB@@LB@Position - mRNA 151 -- 207@LB@Position - sRNA 157 -- 207@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 157 -- 163@LB@
7	0.001816	0.4469	lmo1666	N/A	-21.94	0.003684	151 -- 207	157 -- 207	peptidoglycan-linked protein	lmo0333	_____________150____________208\n_____________\|______________\|\n5'-GUG...AGAUG______________GUAAA...CUC-3'\n______________GGGAUGAAUGAAAA\n______________\|:\|\|\|\|\|\|\|\|\|\|\|\|\n______________CUCUACUUACUUUU\n3'-GUU...GGUUA______________GUUUA...GGA-5'\n_____________\|______________\|\n_____________208____________156\n	201 -- 207	157 -- 163	-18.267 kcal/mol	1.529 kcal/mol	0.904482 kcal/mol	150 208@LB@ \| \|@LB@5'-GUG...AGAUG GUAAA...CUC-3'@LB@ GGGAUGAAUGAAAA@LB@ \|:\|\|\|\|\|\|\|\|\|\|\|\|@LB@ CUCUACUUACUUUU@LB@3'-GUU...GGUUA GUUUA...GGA-5'@LB@ \| \|@LB@ 208 156@LB@@LB@@LB@Energy -21.94 kcal/mol@LB@Hybridization Energy -18.267 kcal/mol@LB@Unfolding Energy - mRNA 1.529 kcal/mol@LB@Unfolding Energy - sRNA 0.904482 kcal/mol@LB@@LB@Position - mRNA 151 -- 207@LB@Position - sRNA 157 -- 207@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 157 -- 163@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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