Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
199	0.03481	0.5559	b0407	yajC	-8.41	0.195839	88 -- 104	15 -- 30	SecYEG protein translocase auxillary subunit	_____________87________________105\n_____________\|_________________\|\n5'-UCU...UUACG_____CUAUU_______GUAAA...UUU-3'\n______________CAAGG_____GAAGAGG\n______________\|\|\|\|\|_____:\|\|\|\|\|\|\n______________GUUCC_____UUUCUCC\n3'-CGC...CUGAA_____CAAU________ACGGC...AAG-5'\n_____________\|_________________\|\n_____________31________________14\n	98 -- 104	15 -- 21	-15.1331 kcal/mol	3.4367 kcal/mol	3.28624 kcal/mol	87 105@LB@ \| \|@LB@5'-UCU...UUACG CUAUU GUAAA...UUU-3'@LB@ CAAGG GAAGAGG@LB@ \|\|\|\|\| :\|\|\|\|\|\|@LB@ GUUCC UUUCUCC@LB@3'-CGC...CUGAA CAAU ACGGC...AAG-5'@LB@ \| \|@LB@ 31 14@LB@@LB@@LB@Energy -8.41 kcal/mol@LB@Hybridization Energy -15.1331 kcal/mol@LB@Unfolding Energy - mRNA 3.4367 kcal/mol@LB@Unfolding Energy - sRNA 3.28624 kcal/mol@LB@@LB@Position - mRNA 88 -- 104@LB@Position - sRNA 15 -- 30@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 15 -- 21@LB@
195	0.03428	0.5559	b0434	yajG	-10.30	0.093744	131 -- 149	66 -- 84	putative lipoprotein	_____________130__________________150\n_____________\|____________________\|\n5'-UGG...UCCCC___C_______AAU______GAUGG...UGC-3'\n______________UCG_CGUUGGC____GUUAU\n______________\|\|:_\|\|\|\|\|\|\|____\|\|\|\|\|\n______________AGU_GCAACCG____CAAUA\n3'-CGC...UUUCA___________AAAU_____AGCUC...AAG-5'\n_____________\|____________________\|\n_____________85___________________65\n	135 -- 141	75 -- 81	-17.0927 kcal/mol	6.21917 kcal/mol	0.5705 kcal/mol	130 150@LB@ \| \|@LB@5'-UGG...UCCCC C AAU GAUGG...UGC-3'@LB@ UCG CGUUGGC GUUAU@LB@ \|\|: \|\|\|\|\|\|\| \|\|\|\|\|@LB@ AGU GCAACCG CAAUA@LB@3'-CGC...UUUCA AAAU AGCUC...AAG-5'@LB@ \| \|@LB@ 85 65@LB@@LB@@LB@Energy -10.30 kcal/mol@LB@Hybridization Energy -17.0927 kcal/mol@LB@Unfolding Energy - mRNA 6.21917 kcal/mol@LB@Unfolding Energy - sRNA 0.5705 kcal/mol@LB@@LB@Position - mRNA 131 -- 149@LB@Position - sRNA 66 -- 84@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 75 -- 81@LB@
179	0.03159	0.5559	b0790	ybhP	-12.03	0.046743	250 -- 264	71 -- 85	endo/exonuclease/phosphatase family protein	_____________249_____________265\n_____________\|_______________\|\n5'-AGU...CAAUA_____AAA_______CCGCG...UUC-3'\n______________UUCAC___GGCUUUA\n______________\|\|\|\|\|___\|\|\|\|\|\|\|\n______________AAGUG___CCGAAAU\n3'-CGC...UUUUC_____CAA_______CAAUA...AAG-5'\n_____________\|_______________\|\n_____________86______________70\n	258 -- 264	71 -- 77	-13.6298 kcal/mol	1.04243 kcal/mol	0.560731 kcal/mol	249 265@LB@ \| \|@LB@5'-AGU...CAAUA AAA CCGCG...UUC-3'@LB@ UUCAC GGCUUUA@LB@ \|\|\|\|\| \|\|\|\|\|\|\|@LB@ AAGUG CCGAAAU@LB@3'-CGC...UUUUC CAA CAAUA...AAG-5'@LB@ \| \|@LB@ 86 70@LB@@LB@@LB@Energy -12.03 kcal/mol@LB@Hybridization Energy -13.6298 kcal/mol@LB@Unfolding Energy - mRNA 1.04243 kcal/mol@LB@Unfolding Energy - sRNA 0.560731 kcal/mol@LB@@LB@Position - mRNA 250 -- 264@LB@Position - sRNA 71 -- 85@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 71 -- 77@LB@
121	0.02058	0.5385	b0796	ybiH	-9.41	0.133616	217 -- 230	24 -- 36	DUF1956 domain-containing tetR family putative transcriptional regulator	_____________216____________231\n_____________\|______________\|\n5'-CGG...UGCCA____AA________GAACA...CCA-3'\n______________UGAC__UCAAGGGU\n______________\|\|\|\|__\|\|\|\|\|\|\|\|\n______________ACUG__AGUUCCCA\n3'-CGC...CCGUC____A_________AUUUU...AAG-5'\n_____________\|______________\|\n_____________37_____________23\n	224 -- 230	24 -- 30	-15.9856 kcal/mol	3.24975 kcal/mol	3.33025 kcal/mol	216 231@LB@ \| \|@LB@5'-CGG...UGCCA AA GAACA...CCA-3'@LB@ UGAC UCAAGGGU@LB@ \|\|\|\| \|\|\|\|\|\|\|\|@LB@ ACUG AGUUCCCA@LB@3'-CGC...CCGUC A AUUUU...AAG-5'@LB@ \| \|@LB@ 37 23@LB@@LB@@LB@Energy -9.41 kcal/mol@LB@Hybridization Energy -15.9856 kcal/mol@LB@Unfolding Energy - mRNA 3.24975 kcal/mol@LB@Unfolding Energy - sRNA 3.33025 kcal/mol@LB@@LB@Position - mRNA 217 -- 230@LB@Position - sRNA 24 -- 36@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 24 -- 30@LB@
21	0.003546	0.5385	b0820	ybiT	-11.98	0.047670	3 -- 23	55 -- 78	ABC-F family putative regulatory ATPase	_____________2________________________24\n_____________\|________________________\|\n_________5'-CC_________GCCA___________AUAUU...UUG-3'\n______________UGGC___AG____UUGGGUUGAGA\n______________\|\|\|\|___\|\|____\|:\|:\|\|\|\|\|\|\|\n______________ACCG___UC____AGCUCAACUCU\n3'-CGC...GUGCA____AAA__AAUA___________UUGAG...AAG-5'\n_____________\|________________________\|\n_____________79_______________________54\n	17 -- 23	55 -- 61	-19.5112 kcal/mol	4.68968 kcal/mol	2.84302 kcal/mol	2 24@LB@ \| \|@LB@ 5'-CC GCCA AUAUU...UUG-3'@LB@ UGGC AG UUGGGUUGAGA@LB@ \|\|\|\| \|\| \|:\|:\|\|\|\|\|\|\|@LB@ ACCG UC AGCUCAACUCU@LB@3'-CGC...GUGCA AAA AAUA UUGAG...AAG-5'@LB@ \| \|@LB@ 79 54@LB@@LB@@LB@Energy -11.98 kcal/mol@LB@Hybridization Energy -19.5112 kcal/mol@LB@Unfolding Energy - mRNA 4.68968 kcal/mol@LB@Unfolding Energy - sRNA 2.84302 kcal/mol@LB@@LB@Position - mRNA 3 -- 23@LB@Position - sRNA 55 -- 78@LB@Position Seed - mRNA 17 -- 23@LB@Position Seed - sRNA 55 -- 61@LB@
128	0.02183	0.5385	b0874	ybjE	-9.13	0.148469	198 -- 230	59 -- 85	putative transporter	_____________197_______________________________231\n_____________\|_________________________________\|\n5'-GGG...GGGUU________UUCUG____GUUAAUC____U____CCCCU...UAA-3'\n______________UUCAUGUU_____GGCU_______AUUC_GGUU\n______________\|\|\|\|:\|\|\|_____\|\|\|\|_______\|\|\|\|_:\|\|\|\n______________AAGUGCAA_____CCGA_______UAAG_UCAA\n3'-CGC...UUUUC_________________AAUCAA_____C____CUCUU...AAG-5'\n_____________\|_________________________________\|\n_____________86________________________________58\n	199 -- 205	78 -- 84	-15.801 kcal/mol	4.95896 kcal/mol	1.70749 kcal/mol	197 231@LB@ \| \|@LB@5'-GGG...GGGUU UUCUG GUUAAUC U CCCCU...UAA-3'@LB@ UUCAUGUU GGCU AUUC GGUU@LB@ \|\|\|\|:\|\|\| \|\|\|\| \|\|\|\| :\|\|\|@LB@ AAGUGCAA CCGA UAAG UCAA@LB@3'-CGC...UUUUC AAUCAA C CUCUU...AAG-5'@LB@ \| \|@LB@ 86 58@LB@@LB@@LB@Energy -9.13 kcal/mol@LB@Hybridization Energy -15.801 kcal/mol@LB@Unfolding Energy - mRNA 4.95896 kcal/mol@LB@Unfolding Energy - sRNA 1.70749 kcal/mol@LB@@LB@Position - mRNA 198 -- 230@LB@Position - sRNA 59 -- 85@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 78 -- 84@LB@
186	0.03285	0.5559	b1003	yccJ	-7.53	0.270746	41 -- 54	75 -- 88	uncharacterized protein	_____________40_____________55\n_____________\|______________\|\n5'-CCU...UUCAC_________GC___ACGCC...GGU-3'\n______________AGGUUCGCG__GGU\n______________\|:\|\|\|\|:\|\|__\|\|:\n______________UUCAAGUGC__CCG\n3'-CGC...GCGUU_________AA___AAAUC...AAG-5'\n_____________\|______________\|\n_____________89_____________74\n	43 -- 49	80 -- 86	-16.6206 kcal/mol	8.57413 kcal/mol	0.515884 kcal/mol	40 55@LB@ \| \|@LB@5'-CCU...UUCAC GC ACGCC...GGU-3'@LB@ AGGUUCGCG GGU@LB@ \|:\|\|\|\|:\|\| \|\|:@LB@ UUCAAGUGC CCG@LB@3'-CGC...GCGUU AA AAAUC...AAG-5'@LB@ \| \|@LB@ 89 74@LB@@LB@@LB@Energy -7.53 kcal/mol@LB@Hybridization Energy -16.6206 kcal/mol@LB@Unfolding Energy - mRNA 8.57413 kcal/mol@LB@Unfolding Energy - sRNA 0.515884 kcal/mol@LB@@LB@Position - mRNA 41 -- 54@LB@Position - sRNA 75 -- 88@LB@Position Seed - mRNA 43 -- 49@LB@Position Seed - sRNA 80 -- 86@LB@
200	0.03481	0.5559	b1058	yceO	-9.40	0.133707	85 -- 100	70 -- 85	uncharacterized protein	_____________84_______________101\n_____________\|________________\|\n5'-CCU...UUAAA_______CA_______GGGAA...GAU-3'\n______________UUUGCGU__GUUUUAG\n______________\|\|::\|\|\|__\|:\|\|\|\|\|\n______________AAGUGCA__CGAAAUC\n3'-CGC...UUUUC_______AC_______AAUAA...AAG-5'\n_____________\|________________\|\n_____________86_______________69\n	94 -- 100	70 -- 76	-11.7699 kcal/mol	1.79867 kcal/mol	0.567379 kcal/mol	84 101@LB@ \| \|@LB@5'-CCU...UUAAA CA GGGAA...GAU-3'@LB@ UUUGCGU GUUUUAG@LB@ \|\|::\|\|\| \|:\|\|\|\|\|@LB@ AAGUGCA CGAAAUC@LB@3'-CGC...UUUUC AC AAUAA...AAG-5'@LB@ \| \|@LB@ 86 69@LB@@LB@@LB@Energy -9.40 kcal/mol@LB@Hybridization Energy -11.7699 kcal/mol@LB@Unfolding Energy - mRNA 1.79867 kcal/mol@LB@Unfolding Energy - sRNA 0.567379 kcal/mol@LB@@LB@Position - mRNA 85 -- 100@LB@Position - sRNA 70 -- 85@LB@Position Seed - mRNA 94 -- 100@LB@Position Seed - sRNA 70 -- 76@LB@
117	0.01966	0.5385	b1203	ychF	-10.24	0.095997	26 -- 41	73 -- 87	catalase inhibitor protein; ATPase K+-dependent ribosome-associated	_____________25_______________42\n_____________\|________________\|\n5'-GAA...AAUGU_______A_A______GACCA...CAU-3'\n______________GGUUUAC_G_UGGCUU\n______________:\|\|\|:\|\|_\|_\|\|\|\|\|\|\n______________UCAAGUG_C_ACCGAA\n3'-CGC...CGUUU_________A______AUCAA...AAG-5'\n_____________\|________________\|\n_____________88_______________72\n	26 -- 32	81 -- 87	-15.1856 kcal/mol	4.42747 kcal/mol	0.51474 kcal/mol	25 42@LB@ \| \|@LB@5'-GAA...AAUGU A A GACCA...CAU-3'@LB@ GGUUUAC G UGGCUU@LB@ :\|\|\|:\|\| \| \|\|\|\|\|\|@LB@ UCAAGUG C ACCGAA@LB@3'-CGC...CGUUU A AUCAA...AAG-5'@LB@ \| \|@LB@ 88 72@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -15.1856 kcal/mol@LB@Unfolding Energy - mRNA 4.42747 kcal/mol@LB@Unfolding Energy - sRNA 0.51474 kcal/mol@LB@@LB@Position - mRNA 26 -- 41@LB@Position - sRNA 73 -- 87@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 81 -- 87@LB@
11	0.002269	0.5385	b1205	ychH	-11.36	0.061344	181 -- 195	75 -- 90	DUF2583 family putative inner membrane protein	_____________180______________196\n_____________\|________________\|\n5'-CAA...ACCCA___________GA___GACAU...UAC-3'\n______________GAAAGUUC_CG__GGU\n______________:\|\|\|\|\|\|\|_\|\|__\|\|:\n______________UUUUCAAG_GC__CCG\n3'-CGC...AGGCG________U__AA___AAAUC...AAG-5'\n_____________\|________________\|\n_____________91_______________74\n	182 -- 188	83 -- 89	-14.0006 kcal/mol	2.12575 kcal/mol	0.517251 kcal/mol	180 196@LB@ \| \|@LB@5'-CAA...ACCCA GA GACAU...UAC-3'@LB@ GAAAGUUC CG GGU@LB@ :\|\|\|\|\|\|\| \|\| \|\|:@LB@ UUUUCAAG GC CCG@LB@3'-CGC...AGGCG U AA AAAUC...AAG-5'@LB@ \| \|@LB@ 91 74@LB@@LB@@LB@Energy -11.36 kcal/mol@LB@Hybridization Energy -14.0006 kcal/mol@LB@Unfolding Energy - mRNA 2.12575 kcal/mol@LB@Unfolding Energy - sRNA 0.517251 kcal/mol@LB@@LB@Position - mRNA 181 -- 195@LB@Position - sRNA 75 -- 90@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 83 -- 89@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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