Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
19	0.003195	0.5385	b3739	atpI	-12.12	0.045044	59 -- 85	62 -- 85	ATP synthase membrane-bound accessory factor	_____________58___________________________86\n_____________\|____________________________\|\n5'-GAA...AUUUA_____CUU______AC____________AAUCA...ACC-3'\n______________UUCAC___UUGGCU__UUA_UUGUUUGA\n______________\|\|\|\|\|___\|\|\|\|\|\|__\|\|\|_\|\|:\|\|:\|\|\n______________AAGUG___AACCGA__AAU_AAUAAGCU\n3'-CGC...UUUUC_____C_____________C________CAACU...AAG-5'\n_____________\|____________________________\|\n_____________86___________________________61\n	79 -- 85	62 -- 68	-17.7596 kcal/mol	3.93623 kcal/mol	1.70582 kcal/mol	58 86@LB@ \| \|@LB@5'-GAA...AUUUA CUU AC AAUCA...ACC-3'@LB@ UUCAC UUGGCU UUA UUGUUUGA@LB@ \|\|\|\|\| \|\|\|\|\|\| \|\|\| \|\|:\|\|:\|\|@LB@ AAGUG AACCGA AAU AAUAAGCU@LB@3'-CGC...UUUUC C C CAACU...AAG-5'@LB@ \| \|@LB@ 86 61@LB@@LB@@LB@Energy -12.12 kcal/mol@LB@Hybridization Energy -17.7596 kcal/mol@LB@Unfolding Energy - mRNA 3.93623 kcal/mol@LB@Unfolding Energy - sRNA 1.70582 kcal/mol@LB@@LB@Position - mRNA 59 -- 85@LB@Position - sRNA 62 -- 85@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 62 -- 68@LB@
127	0.02181	0.5385	b3057	bacA	-7.57	0.267037	62 -- 69	75 -- 82	undecaprenyl pyrophosphate phosphatase	_____________61_______70\n_____________\|________\|\n5'-UUC...GGCGA________GUAAU...UUG-3'\n______________AUGUUGGC\n______________\|:\|\|\|\|\|\|\n______________UGCAACCG\n3'-CGC...UCAAG________AAAUC...AAG-5'\n_____________\|________\|\n_____________83_______74\n	63 -- 69	75 -- 81	-12.0605 kcal/mol	4.15225 kcal/mol	0.339284 kcal/mol	61 70@LB@ \| \|@LB@5'-UUC...GGCGA GUAAU...UUG-3'@LB@ AUGUUGGC@LB@ \|:\|\|\|\|\|\|@LB@ UGCAACCG@LB@3'-CGC...UCAAG AAAUC...AAG-5'@LB@ \| \|@LB@ 83 74@LB@@LB@@LB@Energy -7.57 kcal/mol@LB@Hybridization Energy -12.0605 kcal/mol@LB@Unfolding Energy - mRNA 4.15225 kcal/mol@LB@Unfolding Energy - sRNA 0.339284 kcal/mol@LB@@LB@Position - mRNA 62 -- 69@LB@Position - sRNA 75 -- 82@LB@Position Seed - mRNA 63 -- 69@LB@Position Seed - sRNA 75 -- 81@LB@
133	0.02275	0.5385	b0177	bamA	-6.79	0.350358	265 -- 271	23 -- 29	BamABCDE complex OM biogenesis outer membrane pore-forming assembly factor	_____________264_____272\n_____________\|_______\|\n5'-CUG...UGCUG_______CGUAG...GCC-3'\n______________AAGGGUU\n______________\|\|\|\|\|\|\|\n______________UUCCCAA\n3'-CGC...UGAAG_______UUUUC...AAG-5'\n_____________\|_______\|\n_____________30______22\n	265 -- 271	23 -- 29	-7.93739 kcal/mol	1.09969 kcal/mol	0.0462613 kcal/mol	264 272@LB@ \| \|@LB@5'-CUG...UGCUG CGUAG...GCC-3'@LB@ AAGGGUU@LB@ \|\|\|\|\|\|\|@LB@ UUCCCAA@LB@3'-CGC...UGAAG UUUUC...AAG-5'@LB@ \| \|@LB@ 30 22@LB@@LB@@LB@Energy -6.79 kcal/mol@LB@Hybridization Energy -7.93739 kcal/mol@LB@Unfolding Energy - mRNA 1.09969 kcal/mol@LB@Unfolding Energy - sRNA 0.0462613 kcal/mol@LB@@LB@Position - mRNA 265 -- 271@LB@Position - sRNA 23 -- 29@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 23 -- 29@LB@
58	0.01138	0.5385	b2595	bamD	-7.85	0.240744	21 -- 29	72 -- 80	BamABCDE complex OM biogenesis lipoprotein	_____________20________30\n_____________\|_________\|\n5'-CUG...ACCGA_________CGGAC...AUG-3'\n______________GUUGGUUUU\n______________\|\|\|\|\|:\|\|\|\n______________CAACCGAAA\n3'-CGC...AAGUG_________UCAAU...AAG-5'\n_____________\|_________\|\n_____________81________71\n	23 -- 29	72 -- 78	-10.2023 kcal/mol	2.1418 kcal/mol	0.205868 kcal/mol	20 30@LB@ \| \|@LB@5'-CUG...ACCGA CGGAC...AUG-3'@LB@ GUUGGUUUU@LB@ \|\|\|\|\|:\|\|\|@LB@ CAACCGAAA@LB@3'-CGC...AAGUG UCAAU...AAG-5'@LB@ \| \|@LB@ 81 71@LB@@LB@@LB@Energy -7.85 kcal/mol@LB@Hybridization Energy -10.2023 kcal/mol@LB@Unfolding Energy - mRNA 2.1418 kcal/mol@LB@Unfolding Energy - sRNA 0.205868 kcal/mol@LB@@LB@Position - mRNA 21 -- 29@LB@Position - sRNA 72 -- 80@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 72 -- 78@LB@
9	0.0016	0.5385	b1709	btuD	-9.25	0.141890	185 -- 194	75 -- 84	vitamin B12 ABC transporter ATPase	_____________184________195\n_____________\|__________\|\n5'-GCA...UAAAC_G________AAAAU...GGC-3'\n______________U_GCGUUGGC\n______________\|_:\|\|\|\|\|\|\|\n______________A_UGCAACCG\n3'-CGC...UUUCA_G________AAAUC...AAG-5'\n_____________\|__________\|\n_____________85_________74\n	188 -- 194	75 -- 81	-15.1998 kcal/mol	5.44246 kcal/mol	0.505918 kcal/mol	184 195@LB@ \| \|@LB@5'-GCA...UAAAC G AAAAU...GGC-3'@LB@ U GCGUUGGC@LB@ \| :\|\|\|\|\|\|\|@LB@ A UGCAACCG@LB@3'-CGC...UUUCA G AAAUC...AAG-5'@LB@ \| \|@LB@ 85 74@LB@@LB@@LB@Energy -9.25 kcal/mol@LB@Hybridization Energy -15.1998 kcal/mol@LB@Unfolding Energy - mRNA 5.44246 kcal/mol@LB@Unfolding Energy - sRNA 0.505918 kcal/mol@LB@@LB@Position - mRNA 185 -- 194@LB@Position - sRNA 75 -- 84@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 75 -- 81@LB@
120	0.02045	0.5385	b0979	cbdB	-12.05	0.046235	177 -- 194	16 -- 34	cytochrome bd-II oxidase subunit II	_____________176_________________195\n_____________\|___________________\|\n5'-AUA...ACGCA__AA_______________AAAAU...GCU-3'\n______________GC__CAGGGG_UAAAGGAG\n______________:\|__\|\|:\|\|\|_\|\|\|\|:\|\|\|\n______________UG__GUUCCC_AUUUUCUC\n3'-CGC...GUCAC__AA______A________CACGG...AAG-5'\n_____________\|___________________\|\n_____________35__________________15\n	188 -- 194	16 -- 22	-18.7902 kcal/mol	3.40633 kcal/mol	3.33043 kcal/mol	176 195@LB@ \| \|@LB@5'-AUA...ACGCA AA AAAAU...GCU-3'@LB@ GC CAGGGG UAAAGGAG@LB@ :\| \|\|:\|\|\| \|\|\|\|:\|\|\|@LB@ UG GUUCCC AUUUUCUC@LB@3'-CGC...GUCAC AA A CACGG...AAG-5'@LB@ \| \|@LB@ 35 15@LB@@LB@@LB@Energy -12.05 kcal/mol@LB@Hybridization Energy -18.7902 kcal/mol@LB@Unfolding Energy - mRNA 3.40633 kcal/mol@LB@Unfolding Energy - sRNA 3.33043 kcal/mol@LB@@LB@Position - mRNA 177 -- 194@LB@Position - sRNA 16 -- 34@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 16 -- 22@LB@
105	0.01788	0.5385	b0619	citA	-6.78	0.351941	4 -- 24	64 -- 89	sensory histidine kinase in two-component regulatory system with CitB	_____________3__________________________25\n_____________\|__________________________\|\n________5'-CAU______AG__________________AUUUA...UCA-3'\n______________AGAGUU_____UGGUUUU__UUAUUU\n______________\|:\|\|\|\|_____\|\|\|:\|\|\|__\|\|\|\|\|:\n______________UUUCAA_____ACCGAAA__AAUAAG\n3'-CGC...GGCGU______GUGCA_______UC______CUCAA...AAG-5'\n_____________\|__________________________\|\n_____________90_________________________63\n	12 -- 18	72 -- 78	-10.9037 kcal/mol	3.54899 kcal/mol	0.576654 kcal/mol	3 25@LB@ \| \|@LB@ 5'-CAU AG AUUUA...UCA-3'@LB@ AGAGUU UGGUUUU UUAUUU@LB@ \|:\|\|\|\| \|\|\|:\|\|\| \|\|\|\|\|:@LB@ UUUCAA ACCGAAA AAUAAG@LB@3'-CGC...GGCGU GUGCA UC CUCAA...AAG-5'@LB@ \| \|@LB@ 90 63@LB@@LB@@LB@Energy -6.78 kcal/mol@LB@Hybridization Energy -10.9037 kcal/mol@LB@Unfolding Energy - mRNA 3.54899 kcal/mol@LB@Unfolding Energy - sRNA 0.576654 kcal/mol@LB@@LB@Position - mRNA 4 -- 24@LB@Position - sRNA 64 -- 89@LB@Position Seed - mRNA 12 -- 18@LB@Position Seed - sRNA 72 -- 78@LB@
91	0.0156	0.5385	b0881	clpS	-13.09	0.030255	248 -- 265	74 -- 90	regulatory protein for ClpA substrate specificity	_____________247________________266\n_____________\|__________________\|\n5'-GCA...GAAGA___________AC_C___AAAAC...UCA-3'\n______________AAAAGUUCGCG__G_GCU\n______________\|\|\|\|\|\|\|\|:\|\|__\|_\|\|\|\n______________UUUUCAAGUGC__C_CGA\n3'-CGC...AGGCG___________AA_____AAUCA...AAG-5'\n_____________\|__________________\|\n_____________91_________________73\n	252 -- 258	80 -- 86	-16.9257 kcal/mol	3.31752 kcal/mol	0.517587 kcal/mol	247 266@LB@ \| \|@LB@5'-GCA...GAAGA AC C AAAAC...UCA-3'@LB@ AAAAGUUCGCG G GCU@LB@ \|\|\|\|\|\|\|\|:\|\| \| \|\|\|@LB@ UUUUCAAGUGC C CGA@LB@3'-CGC...AGGCG AA AAUCA...AAG-5'@LB@ \| \|@LB@ 91 73@LB@@LB@@LB@Energy -13.09 kcal/mol@LB@Hybridization Energy -16.9257 kcal/mol@LB@Unfolding Energy - mRNA 3.31752 kcal/mol@LB@Unfolding Energy - sRNA 0.517587 kcal/mol@LB@@LB@Position - mRNA 248 -- 265@LB@Position - sRNA 74 -- 90@LB@Position Seed - mRNA 252 -- 258@LB@Position Seed - sRNA 80 -- 86@LB@
198	0.03475	0.5559	b1249	clsA	-8.08	0.221640	216 -- 230	57 -- 82	cardiolipin synthase 1	_____________215________________________231\n_____________\|__________________________\|\n5'-GUC...UUUAU__________________________UUGGC...CAG-3'\n______________ACGUUGGU___________GAGUUGG\n______________\|\|\|\|\|\|\|:___________\|\|\|\|\|\|:\n______________UGCAACCG___________CUCAACU\n3'-CGC...UCAAG________AAAUCAAUAAG_______CUUUG...AAG-5'\n_____________\|__________________________\|\n_____________83_________________________56\n	224 -- 230	57 -- 63	-16.5635 kcal/mol	7.18735 kcal/mol	1.29708 kcal/mol	215 231@LB@ \| \|@LB@5'-GUC...UUUAU UUGGC...CAG-3'@LB@ ACGUUGGU GAGUUGG@LB@ \|\|\|\|\|\|\|: \|\|\|\|\|\|:@LB@ UGCAACCG CUCAACU@LB@3'-CGC...UCAAG AAAUCAAUAAG CUUUG...AAG-5'@LB@ \| \|@LB@ 83 56@LB@@LB@@LB@Energy -8.08 kcal/mol@LB@Hybridization Energy -16.5635 kcal/mol@LB@Unfolding Energy - mRNA 7.18735 kcal/mol@LB@Unfolding Energy - sRNA 1.29708 kcal/mol@LB@@LB@Position - mRNA 216 -- 230@LB@Position - sRNA 57 -- 82@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 57 -- 63@LB@
84	0.01516	0.5385	b1871	cmoB	-9.91	0.109770	166 -- 183	71 -- 87	tRNA (cmo5U34)-carboxymethyltransferase carboxy-SAM-dependent	_____________165________________184\n_____________\|__________________\|\n5'-CAC...ACUUU_______U_G____A___AAAGC...AGC-3'\n______________GGUUCAC_G_UGGC_UUA\n______________:\|\|\|\|\|\|_\|_\|\|\|\|_\|\|\|\n______________UCAAGUG_C_ACCG_AAU\n3'-CGC...CGUUU_________A____A___CAAUA...AAG-5'\n_____________\|__________________\|\n_____________88_________________70\n	166 -- 172	81 -- 87	-14.4863 kcal/mol	4.01825 kcal/mol	0.562376 kcal/mol	165 184@LB@ \| \|@LB@5'-CAC...ACUUU U G A AAAGC...AGC-3'@LB@ GGUUCAC G UGGC UUA@LB@ :\|\|\|\|\|\| \| \|\|\|\| \|\|\|@LB@ UCAAGUG C ACCG AAU@LB@3'-CGC...CGUUU A A CAAUA...AAG-5'@LB@ \| \|@LB@ 88 70@LB@@LB@@LB@Energy -9.91 kcal/mol@LB@Hybridization Energy -14.4863 kcal/mol@LB@Unfolding Energy - mRNA 4.01825 kcal/mol@LB@Unfolding Energy - sRNA 0.562376 kcal/mol@LB@@LB@Position - mRNA 166 -- 183@LB@Position - sRNA 71 -- 87@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 81 -- 87@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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