Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
197	0.0346	0.5559	b2829	ptsP	-9.94	0.108120	103 -- 113	80 -- 90	PEP-protein phosphotransferase enzyme I; GAF domain containing protein	_____________102_________114\n_____________\|___________\|\n5'-CCA...AAUGA___________AGUGU...UGG-3'\n______________AAGAGUUCGCG\n______________\|\|:\|\|\|\|\|:\|\|\n______________UUUUCAAGUGC\n3'-CGC...AGGCG___________AACCG...AAG-5'\n_____________\|___________\|\n_____________91__________79\n	107 -- 113	80 -- 86	-13.2227 kcal/mol	2.77203 kcal/mol	0.506776 kcal/mol	102 114@LB@ \| \|@LB@5'-CCA...AAUGA AGUGU...UGG-3'@LB@ AAGAGUUCGCG@LB@ \|\|:\|\|\|\|\|:\|\|@LB@ UUUUCAAGUGC@LB@3'-CGC...AGGCG AACCG...AAG-5'@LB@ \| \|@LB@ 91 79@LB@@LB@@LB@Energy -9.94 kcal/mol@LB@Hybridization Energy -13.2227 kcal/mol@LB@Unfolding Energy - mRNA 2.77203 kcal/mol@LB@Unfolding Energy - sRNA 0.506776 kcal/mol@LB@@LB@Position - mRNA 103 -- 113@LB@Position - sRNA 80 -- 90@LB@Position Seed - mRNA 107 -- 113@LB@Position Seed - sRNA 80 -- 86@LB@
174	0.03067	0.5559	b4005	purD	-11.17	0.066076	4 -- 22	73 -- 90	phosphoribosylglycinamide synthetase phosphoribosylamine-glycine ligase	_____________3___________________23\n_____________\|___________________\|\n________5'-GUG________CU__A______CUGAC...GUA-3'\n______________AAAGGUUC__CG_UGGCUU\n______________\|\|\|:\|\|\|\|__\|\|_\|\|\|\|\|\|\n______________UUUUCAAG__GC_ACCGAA\n3'-CGC...AGGCG________U___A______AUCAA...AAG-5'\n_____________\|___________________\|\n_____________91__________________72\n	5 -- 11	83 -- 89	-16.9563 kcal/mol	5.26463 kcal/mol	0.517799 kcal/mol	3 23@LB@ \| \|@LB@ 5'-GUG CU A CUGAC...GUA-3'@LB@ AAAGGUUC CG UGGCUU@LB@ \|\|\|:\|\|\|\| \|\| \|\|\|\|\|\|@LB@ UUUUCAAG GC ACCGAA@LB@3'-CGC...AGGCG U A AUCAA...AAG-5'@LB@ \| \|@LB@ 91 72@LB@@LB@@LB@Energy -11.17 kcal/mol@LB@Hybridization Energy -16.9563 kcal/mol@LB@Unfolding Energy - mRNA 5.26463 kcal/mol@LB@Unfolding Energy - sRNA 0.517799 kcal/mol@LB@@LB@Position - mRNA 4 -- 22@LB@Position - sRNA 73 -- 90@LB@Position Seed - mRNA 5 -- 11@LB@Position Seed - sRNA 83 -- 89@LB@
103	0.01739	0.5385	b0945	pyrD	-8.62	0.180746	193 -- 204	79 -- 90	dihydro-orotate oxidase FMN-linked	_____________192__________205\n_____________\|____________\|\n5'-UGU...UCCAG____________ACUAC...CGU-3'\n______________GAGAGUUCAUGU\n______________:\|:\|\|\|\|\|\|:\|\|\n______________UUUUCAAGUGCA\n3'-CGC...AGGCG____________ACCGA...AAG-5'\n_____________\|____________\|\n_____________91___________78\n	198 -- 204	79 -- 85	-13.8118 kcal/mol	4.6828 kcal/mol	0.506924 kcal/mol	192 205@LB@ \| \|@LB@5'-UGU...UCCAG ACUAC...CGU-3'@LB@ GAGAGUUCAUGU@LB@ :\|:\|\|\|\|\|\|:\|\|@LB@ UUUUCAAGUGCA@LB@3'-CGC...AGGCG ACCGA...AAG-5'@LB@ \| \|@LB@ 91 78@LB@@LB@@LB@Energy -8.62 kcal/mol@LB@Hybridization Energy -13.8118 kcal/mol@LB@Unfolding Energy - mRNA 4.6828 kcal/mol@LB@Unfolding Energy - sRNA 0.506924 kcal/mol@LB@@LB@Position - mRNA 193 -- 204@LB@Position - sRNA 79 -- 90@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 79 -- 85@LB@
193	0.03391	0.5559	b3642	pyrE	-10.20	0.097546	70 -- 90	57 -- 80	orotate phosphoribosyltransferase	_____________69_______________________91\n_____________\|________________________\|\n5'-CGG...AUCUU_______C___CC_______GG__AUCGA...AUU-3'\n______________GUUGGCU_UGG_____CGAG__GA\n______________\|\|\|\|\|\|\|_\|:\|_____\|\|\|\|__\|\|\n______________CAACCGA_AUC_____GCUC__CU\n3'-CGC...AAGUG_______A___AAUAA____AA__CUUUG...AAG-5'\n_____________\|________________________\|\n_____________81_______________________56\n	70 -- 76	74 -- 80	-18.9167 kcal/mol	7.49492 kcal/mol	1.21861 kcal/mol	69 91@LB@ \| \|@LB@5'-CGG...AUCUU C CC GG AUCGA...AUU-3'@LB@ GUUGGCU UGG CGAG GA@LB@ \|\|\|\|\|\|\| \|:\| \|\|\|\| \|\|@LB@ CAACCGA AUC GCUC CU@LB@3'-CGC...AAGUG A AAUAA AA CUUUG...AAG-5'@LB@ \| \|@LB@ 81 56@LB@@LB@@LB@Energy -10.20 kcal/mol@LB@Hybridization Energy -18.9167 kcal/mol@LB@Unfolding Energy - mRNA 7.49492 kcal/mol@LB@Unfolding Energy - sRNA 1.21861 kcal/mol@LB@@LB@Position - mRNA 70 -- 90@LB@Position - sRNA 57 -- 80@LB@Position Seed - mRNA 70 -- 76@LB@Position Seed - sRNA 74 -- 80@LB@
15	0.002894	0.5385	b2780	pyrG	-11.46	0.058961	111 -- 140	60 -- 90	CTP synthetase	_____________110_______________________________141\n_____________\|_________________________________\|\n5'-UGU...AUAAU________________ACA___CA_________AUACU...GCC-3'\n______________GAAAGUUUG___UGGC___GGU__UGUUCGGGU\n______________:\|\|\|\|\|\|::___\|\|\|\|___:\|\|__\|:\|\|\|\|:\|\|\n______________UUUUCAAGU___ACCG___UCA__AUAAGCUCA\n3'-CGC...AGGCG_________GCA____AAA______________ACUCU...AAG-5'\n_____________\|_________________________________\|\n_____________91________________________________59\n	134 -- 140	60 -- 66	-21.5125 kcal/mol	8.34782 kcal/mol	1.70935 kcal/mol	110 141@LB@ \| \|@LB@5'-UGU...AUAAU ACA CA AUACU...GCC-3'@LB@ GAAAGUUUG UGGC GGU UGUUCGGGU@LB@ :\|\|\|\|\|\|:: \|\|\|\| :\|\| \|:\|\|\|\|:\|\|@LB@ UUUUCAAGU ACCG UCA AUAAGCUCA@LB@3'-CGC...AGGCG GCA AAA ACUCU...AAG-5'@LB@ \| \|@LB@ 91 59@LB@@LB@@LB@Energy -11.46 kcal/mol@LB@Hybridization Energy -21.5125 kcal/mol@LB@Unfolding Energy - mRNA 8.34782 kcal/mol@LB@Unfolding Energy - sRNA 1.70935 kcal/mol@LB@@LB@Position - mRNA 111 -- 140@LB@Position - sRNA 60 -- 90@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 60 -- 66@LB@
163	0.02879	0.5559	b4244	pyrI	-5.82	0.477897	276 -- 293	60 -- 77	aspartate carbamoyltransferase regulatory subunit	_____________275________________294\n_____________\|__________________\|\n5'-UUC...AGAUC_______AGCUG______CUGUUCA-3'\n______________GGUUUUA_____UUGAGU\n______________\|\|:\|\|\|\|_____\|:\|\|\|\|\n______________CCGAAAU_____AGCUCA\n3'-CGC...UGCAA_______CAAUA______ACUCU...AAG-5'\n_____________\|__________________\|\n_____________78_________________59\n	276 -- 282	71 -- 77	-12.7499 kcal/mol	6.13118 kcal/mol	0.796465 kcal/mol	275 294@LB@ \| \|@LB@5'-UUC...AGAUC AGCUG CUGUUCA-3'@LB@ GGUUUUA UUGAGU@LB@ \|\|:\|\|\|\| \|:\|\|\|\|@LB@ CCGAAAU AGCUCA@LB@3'-CGC...UGCAA CAAUA ACUCU...AAG-5'@LB@ \| \|@LB@ 78 59@LB@@LB@@LB@Energy -5.82 kcal/mol@LB@Hybridization Energy -12.7499 kcal/mol@LB@Unfolding Energy - mRNA 6.13118 kcal/mol@LB@Unfolding Energy - sRNA 0.796465 kcal/mol@LB@@LB@Position - mRNA 276 -- 293@LB@Position - sRNA 60 -- 77@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 71 -- 77@LB@
140	0.02329	0.5385	b3026	qseC	-7.47	0.277092	174 -- 202	52 -- 78	quorum sensing sensory histidine kinase in two-component regulatory system with QseB	_____________173___________________________203\n_____________\|_____________________________\|\n5'-AAA...GUGCA____A_______CA_AUU__G________GAAAU...GGA-3'\n______________UGGU_UUGGUUA__C___AG_UGAGAAAU\n______________\|\|\|:_\|\|:\|\|\|\|__\|___\|\|_\|\|\|\|\|\|\|:\n______________ACCG_AAUCAAU__G___UC_ACUCUUUG\n3'-CGC...GUGCA____A_______AA_C____A________AGAGC...AAG-5'\n_____________\|_____________________________\|\n_____________79____________________________51\n	196 -- 202	52 -- 58	-18.6962 kcal/mol	6.8409 kcal/mol	4.38944 kcal/mol	173 203@LB@ \| \|@LB@5'-AAA...GUGCA A CA AUU G GAAAU...GGA-3'@LB@ UGGU UUGGUUA C AG UGAGAAAU@LB@ \|\|\|: \|\|:\|\|\|\| \| \|\| \|\|\|\|\|\|\|:@LB@ ACCG AAUCAAU G UC ACUCUUUG@LB@3'-CGC...GUGCA A AA C A AGAGC...AAG-5'@LB@ \| \|@LB@ 79 51@LB@@LB@@LB@Energy -7.47 kcal/mol@LB@Hybridization Energy -18.6962 kcal/mol@LB@Unfolding Energy - mRNA 6.8409 kcal/mol@LB@Unfolding Energy - sRNA 4.38944 kcal/mol@LB@@LB@Position - mRNA 174 -- 202@LB@Position - sRNA 52 -- 78@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 52 -- 58@LB@
61	0.01179	0.5385	b3748	rbsD	-10.58	0.083988	129 -- 141	72 -- 84	D-ribose pyranase	_____________128___________142\n_____________\|_____________\|\n5'-GGU...UAACG_____A_______CCCAA...UCC-3'\n______________UCACG_UGGUUUU\n______________\|\|\|\|\|_\|\|\|:\|\|\|\n______________AGUGC_ACCGAAA\n3'-CGC...UUUCA_____A_______UCAAU...AAG-5'\n_____________\|_____________\|\n_____________85____________71\n	135 -- 141	72 -- 78	-14.1499 kcal/mol	3.06287 kcal/mol	0.509125 kcal/mol	128 142@LB@ \| \|@LB@5'-GGU...UAACG A CCCAA...UCC-3'@LB@ UCACG UGGUUUU@LB@ \|\|\|\|\| \|\|\|:\|\|\|@LB@ AGUGC ACCGAAA@LB@3'-CGC...UUUCA A UCAAU...AAG-5'@LB@ \| \|@LB@ 85 71@LB@@LB@@LB@Energy -10.58 kcal/mol@LB@Hybridization Energy -14.1499 kcal/mol@LB@Unfolding Energy - mRNA 3.06287 kcal/mol@LB@Unfolding Energy - sRNA 0.509125 kcal/mol@LB@@LB@Position - mRNA 129 -- 141@LB@Position - sRNA 72 -- 84@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 72 -- 78@LB@
114	0.01931	0.5385	b1951	rcsA	-10.71	0.079523	269 -- 285	16 -- 29	transcriptional regulator of colanic acid capsular biosynthesis	_____________268_______________286\n_____________\|_________________\|\n5'-GUU...AGUAG__________AAA____AAAUC...UUG-3'\n______________AGGGGUUAAA___AGAG\n______________\|:\|\|\|\|\|\|\|\|___\|\|\|\|\n______________UUCCCAAUUU___UCUC\n3'-CGC...UGAAG_________________CACGG...AAG-5'\n_____________\|_________________\|\n_____________30________________15\n	272 -- 278	20 -- 26	-15.5636 kcal/mol	1.76121 kcal/mol	3.08831 kcal/mol	268 286@LB@ \| \|@LB@5'-GUU...AGUAG AAA AAAUC...UUG-3'@LB@ AGGGGUUAAA AGAG@LB@ \|:\|\|\|\|\|\|\|\| \|\|\|\|@LB@ UUCCCAAUUU UCUC@LB@3'-CGC...UGAAG CACGG...AAG-5'@LB@ \| \|@LB@ 30 15@LB@@LB@@LB@Energy -10.71 kcal/mol@LB@Hybridization Energy -15.5636 kcal/mol@LB@Unfolding Energy - mRNA 1.76121 kcal/mol@LB@Unfolding Energy - sRNA 3.08831 kcal/mol@LB@@LB@Position - mRNA 269 -- 285@LB@Position - sRNA 16 -- 29@LB@Position Seed - mRNA 272 -- 278@LB@Position Seed - sRNA 20 -- 26@LB@
109	0.0187	0.5385	b2616	recN	-10.46	0.088138	200 -- 208	75 -- 83	recombination and repair protein	_____________199_______209\n_____________\|_________\|\n5'-UGA...ACGAC_________ACAAC...CGG-3'\n______________UAUGUUGGC\n______________:\|:\|\|\|\|\|\|\n______________GUGCAACCG\n3'-CGC...UUCAA_________AAAUC...AAG-5'\n_____________\|_________\|\n_____________84________74\n	202 -- 208	75 -- 81	-13.4657 kcal/mol	2.51607 kcal/mol	0.492259 kcal/mol	199 209@LB@ \| \|@LB@5'-UGA...ACGAC ACAAC...CGG-3'@LB@ UAUGUUGGC@LB@ :\|:\|\|\|\|\|\|@LB@ GUGCAACCG@LB@3'-CGC...UUCAA AAAUC...AAG-5'@LB@ \| \|@LB@ 84 74@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -13.4657 kcal/mol@LB@Unfolding Energy - mRNA 2.51607 kcal/mol@LB@Unfolding Energy - sRNA 0.492259 kcal/mol@LB@@LB@Position - mRNA 200 -- 208@LB@Position - sRNA 75 -- 83@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 75 -- 81@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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