Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
160	0.02589	0.526	b3297	rpsK	-10.33	0.083340	178 -- 193	52 -- 64	30S ribosomal subunit protein S11		_____________177______________194\n_____________\|________________\|\n5'-CGA...AUCAA______AUC_______UUGAA...CUA-3'\n______________GAAAUA___GGGGUGA\n______________\|\|\|\|\|\|___\|\|\|\|\|:\|\n______________CUUUAU___CCCCAUU\n3'-UUU...GCCAA________________UGUCU...UCG-5'\n_____________\|________________\|\n_____________65_______________51\n	187 -- 193	52 -- 58	-13.1856 kcal/mol	2.77978 kcal/mol	0.0778991 kcal/mol	177 194@LB@ \| \|@LB@5'-CGA...AUCAA AUC UUGAA...CUA-3'@LB@ GAAAUA GGGGUGA@LB@ \|\|\|\|\|\| \|\|\|\|\|:\|@LB@ CUUUAU CCCCAUU@LB@3'-UUU...GCCAA UGUCU...UCG-5'@LB@ \| \|@LB@ 65 51@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -13.1856 kcal/mol@LB@Unfolding Energy - mRNA 2.77978 kcal/mol@LB@Unfolding Energy - sRNA 0.0778991 kcal/mol@LB@@LB@Position - mRNA 178 -- 193@LB@Position - sRNA 52 -- 64@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 52 -- 58@LB@
159	0.02583	0.526	b2572	rseA	-12.83	0.023837	184 -- 236	1 -- 63	anti-sigma factor		_____________183_____________________________________________________________237\n_____________\|_______________________________________________________________\|\n5'-UCC...AUACU_____A_____U________G__________CA__UUUCCGCU____________________GAAAC...GGG-3'\n______________GGAUA_GGGUA__UAGGCAU_CAGAAA_GAA__AC______________UUAAUGG__AUGGC\n______________::\|\|\|_\|\|\|\|\|__:\|\|:\|\|\|_\|\|\|\|\|\|_\|\|\|__\|\|______________\|\|\|\|\|\|:__\|\|:\|\|\n______________UUUAU_CCCAU__GUCUGUA_GUCUUU_CUU__UG______________AAUUACU__UAUCG\n3'-UUU...CCAAC_____C_____UU_______A______A___AC__CCAUUAUUUAUUUC_______AC_____-5'\n_____________\|______________________________________________________________\|\n_____________64_____________________________________________________________1\n	225 -- 231	8 -- 14	-31.1691 kcal/mol	11.9981 kcal/mol	6.344 kcal/mol	183 237@LB@ \| \|@LB@5'-UCC...AUACU A U G CA UUUCCGCU GAAAC...GGG-3'@LB@ GGAUA GGGUA UAGGCAU CAGAAA GAA AC UUAAUGG AUGGC@LB@ ::\|\|\| \|\|\|\|\| :\|\|:\|\|\| \|\|\|\|\|\| \|\|\| \|\| \|\|\|\|\|\|: \|\|:\|\|@LB@ UUUAU CCCAU GUCUGUA GUCUUU CUU UG AAUUACU UAUCG@LB@3'-UUU...CCAAC C UU A A AC CCAUUAUUUAUUUC AC -5'@LB@ \| \|@LB@ 64 1@LB@@LB@@LB@Energy -12.83 kcal/mol@LB@Hybridization Energy -31.1691 kcal/mol@LB@Unfolding Energy - mRNA 11.9981 kcal/mol@LB@Unfolding Energy - sRNA 6.344 kcal/mol@LB@@LB@Position - mRNA 184 -- 236@LB@Position - sRNA 1 -- 63@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 8 -- 14@LB@
166	0.02659	0.526	b1580	rspB	-9.76	0.108040	184 -- 209	1 -- 29	putative Zn-dependent NAD(P)-binding oxidoreductase		_____________183___________________________210\n_____________\|_____________________________\|\n5'-AUG...GGAAC______G_______A_______AA_____AUAUU...UUG-3'\n______________UGGUAA_GAGUAAG__UAAUGA____AGC\n______________:\|\|\|\|\|_:\|:\|\|\|:__\|\|\|\|\|\|____\|\|\|\n______________GCCAUU_UUUAUUU__AUUACU____UCG\n3'-UUU...UUACU______A_______CA______ACUA___-5'\n_____________\|____________________________\|\n_____________30___________________________1\n	191 -- 197	16 -- 22	-16.0836 kcal/mol	4.192 kcal/mol	2.12711 kcal/mol	183 210@LB@ \| \|@LB@5'-AUG...GGAAC G A AA AUAUU...UUG-3'@LB@ UGGUAA GAGUAAG UAAUGA AGC@LB@ :\|\|\|\|\| :\|:\|\|\|: \|\|\|\|\|\| \|\|\|@LB@ GCCAUU UUUAUUU AUUACU UCG@LB@3'-UUU...UUACU A CA ACUA -5'@LB@ \| \|@LB@ 30 1@LB@@LB@@LB@Energy -9.76 kcal/mol@LB@Hybridization Energy -16.0836 kcal/mol@LB@Unfolding Energy - mRNA 4.192 kcal/mol@LB@Unfolding Energy - sRNA 2.12711 kcal/mol@LB@@LB@Position - mRNA 184 -- 209@LB@Position - sRNA 1 -- 29@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 16 -- 22@LB@
98	0.01586	0.5059	b1860	ruvB	-8.95	0.154425	184 -- 211	1 -- 30	ATP-dependent DNA helicase component of RuvABC resolvasome		_____________183_____________________________212\n_____________\|_______________________________\|\n5'-ACG...GCGCC___U__UG_______________U_______AGACC...GUC-3'\n______________GCG_UA__AGGUAAAG__GAUGA_UGA_AGC\n______________:\|\|_\|\|__\|::\|\|\|\|\|__:\|\|\|\|_\|\|\|_\|\|\|\n______________UGC_AU__UUUAUUUC__UUACU_ACU_UCG\n3'-UUU...CUUAC___C__UA________AA_________A___-5'\n_____________\|______________________________\|\n_____________31_____________________________1\n	193 -- 199	15 -- 21	-17.0174 kcal/mol	5.93551 kcal/mol	2.12822 kcal/mol	183 212@LB@ \| \|@LB@5'-ACG...GCGCC U UG U AGACC...GUC-3'@LB@ GCG UA AGGUAAAG GAUGA UGA AGC@LB@ :\|\| \|\| \|::\|\|\|\|\| :\|\|\|\| \|\|\| \|\|\|@LB@ UGC AU UUUAUUUC UUACU ACU UCG@LB@3'-UUU...CUUAC C UA AA A -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -8.95 kcal/mol@LB@Hybridization Energy -17.0174 kcal/mol@LB@Unfolding Energy - mRNA 5.93551 kcal/mol@LB@Unfolding Energy - sRNA 2.12822 kcal/mol@LB@@LB@Position - mRNA 184 -- 211@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 15 -- 21@LB@
32	0.003146	0.3086	b1553	rzpQ	-12.95	0.022283	187 -- 223	23 -- 60	Rz-like protein Qin prophage		_____________186______________________________________224\n_____________\|________________________________________\|\n5'-UAU...CCUGC______AUA______UGAA_____UAUUC___________UUUUC...GUG-3'\n______________UGGGGG___GAACAG____UCAGA________AUGGUGAU\n______________\|:\|\|\|\|___:\|\|\|\|\|____\|\|\|\|\|________\|:\|\|\|:\|\|\n______________AUCCCC___UUUGUC____AGUCU________UGCCAUUA\n3'-UUU...ACUUU______A________UGUA_____UUACUUAC________UUUAU...UCG-5'\n_____________\|________________________________________\|\n_____________61_______________________________________22\n	217 -- 223	23 -- 29	-27.4351 kcal/mol	8.1631 kcal/mol	6.31885 kcal/mol	186 224@LB@ \| \|@LB@5'-UAU...CCUGC AUA UGAA UAUUC UUUUC...GUG-3'@LB@ UGGGGG GAACAG UCAGA AUGGUGAU@LB@ \|:\|\|\|\| :\|\|\|\|\| \|\|\|\|\| \|:\|\|\|:\|\|@LB@ AUCCCC UUUGUC AGUCU UGCCAUUA@LB@3'-UUU...ACUUU A UGUA UUACUUAC UUUAU...UCG-5'@LB@ \| \|@LB@ 61 22@LB@@LB@@LB@Energy -12.95 kcal/mol@LB@Hybridization Energy -27.4351 kcal/mol@LB@Unfolding Energy - mRNA 8.1631 kcal/mol@LB@Unfolding Energy - sRNA 6.31885 kcal/mol@LB@@LB@Position - mRNA 187 -- 223@LB@Position - sRNA 23 -- 60@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 23 -- 29@LB@
16	0.0008012	0.1482	b2011	sbcB	-16.25	0.003318	186 -- 217	24 -- 52	exodeoxyribonuclease I; exonuclease I		_____________185______________________________218\n_____________\|________________________________\|\n5'-CCU...GAAAU_______UUAACCUAAU_______________GCAAC...CCA-3'\n______________AACGGAU__________GAUGAAUGACGGUAA\n______________\|\|\|:\|\|:__________:\|\|\|\|\|\|\|\|\|\|\|\|\|\|\n______________UUGUCUG__________UUACUUACUGCCAUU\n3'-UUU...CCCAU_______UAAGUCU__________________AUUUA...UCG-5'\n_____________\|________________________________\|\n_____________53_______________________________23\n	211 -- 217	24 -- 30	-27.0341 kcal/mol	4.49086 kcal/mol	6.2974 kcal/mol	185 218@LB@ \| \|@LB@5'-CCU...GAAAU UUAACCUAAU GCAAC...CCA-3'@LB@ AACGGAU GAUGAAUGACGGUAA@LB@ \|\|\|:\|\|: :\|\|\|\|\|\|\|\|\|\|\|\|\|\|@LB@ UUGUCUG UUACUUACUGCCAUU@LB@3'-UUU...CCCAU UAAGUCU AUUUA...UCG-5'@LB@ \| \|@LB@ 53 23@LB@@LB@@LB@Energy -16.25 kcal/mol@LB@Hybridization Energy -27.0341 kcal/mol@LB@Unfolding Energy - mRNA 4.49086 kcal/mol@LB@Unfolding Energy - sRNA 6.2974 kcal/mol@LB@@LB@Position - mRNA 186 -- 217@LB@Position - sRNA 24 -- 52@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 24 -- 30@LB@
7	0.0001529	0.07172	b0687	seqA	-15.48	0.005290	212 -- 226	5 -- 19	negative modulator of initiation of replication		_____________211_____________227\n_____________\|_______________\|\n5'-GGA...ACGAU_______________ACUCU...UGA-3'\n______________UGAAGUUGAUGAUGA\n______________\|:\|\|\|\|\|:\|\|\|\|\|\|\|\n______________AUUUCAAUUACUACU\n3'-UUU...UAUUU_______________AUCG-5'\n_____________\|_______________\|\n_____________20______________4\n	220 -- 226	5 -- 11	-18.3215 kcal/mol	2.77487 kcal/mol	0.0620344 kcal/mol	211 227@LB@ \| \|@LB@5'-GGA...ACGAU ACUCU...UGA-3'@LB@ UGAAGUUGAUGAUGA@LB@ \|:\|\|\|\|\|:\|\|\|\|\|\|\|@LB@ AUUUCAAUUACUACU@LB@3'-UUU...UAUUU AUCG-5'@LB@ \| \|@LB@ 20 4@LB@@LB@@LB@Energy -15.48 kcal/mol@LB@Hybridization Energy -18.3215 kcal/mol@LB@Unfolding Energy - mRNA 2.77487 kcal/mol@LB@Unfolding Energy - sRNA 0.0620344 kcal/mol@LB@@LB@Position - mRNA 212 -- 226@LB@Position - sRNA 5 -- 19@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 5 -- 11@LB@
100	0.01591	0.5059	b0531	sfmC	-11.86	0.039421	211 -- 227	6 -- 22	putative periplasmic pilus chaperone	b4316	_____________210_______________228\n_____________\|_________________\|\n5'-AAU...GACUA___________U_____CUCAU...GUA-3'\n______________AAAUAAAGUUA_UGAUG\n______________\|\|\|\|\|\|\|\|\|\|\|_\|\|\|\|\|\n______________UUUAUUUCAAU_ACUAC\n3'-UUU...CAUUA___________U_____UAUCG-5'\n_____________\|_________________\|\n_____________23________________5\n	215 -- 221	12 -- 18	-15.888 kcal/mol	3.9302 kcal/mol	0.0976382 kcal/mol	210 228@LB@ \| \|@LB@5'-AAU...GACUA U CUCAU...GUA-3'@LB@ AAAUAAAGUUA UGAUG@LB@ \|\|\|\|\|\|\|\|\|\|\| \|\|\|\|\|@LB@ UUUAUUUCAAU ACUAC@LB@3'-UUU...CAUUA U UAUCG-5'@LB@ \| \|@LB@ 23 5@LB@@LB@@LB@Energy -11.86 kcal/mol@LB@Hybridization Energy -15.888 kcal/mol@LB@Unfolding Energy - mRNA 3.9302 kcal/mol@LB@Unfolding Energy - sRNA 0.0976382 kcal/mol@LB@@LB@Position - mRNA 211 -- 227@LB@Position - sRNA 6 -- 22@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 12 -- 18@LB@
116	0.01846	0.5155	b3349	slyD	-11.19	0.055067	245 -- 274	8 -- 47	FKBP-type peptidyl prolyl cis-trans isomerase (rotamase)		_____________244______________________________________275\n_____________\|________________________________________\|\n5'-UGU...CAGGU____A__________________UGUU_____________GUCUC...ACU-3'\n______________ACGU_CAGAA______GACGGUG________GGUUGAUGA\n______________\|\|:\|_\|\|\|\|\|______\|\|\|\|\|\|:________:\|\|\|:\|\|\|\|\n______________UGUA_GUCUU______CUGCCAU________UCAAUUACU\n3'-UUU...UUGUC____A_____UACUUA_______UAUUUAUU_________ACUAUCG-5'\n_____________\|________________________________________\|\n_____________48_______________________________________7\n	268 -- 274	8 -- 14	-23.1983 kcal/mol	5.6913 kcal/mol	6.31565 kcal/mol	244 275@LB@ \| \|@LB@5'-UGU...CAGGU A UGUU GUCUC...ACU-3'@LB@ ACGU CAGAA GACGGUG GGUUGAUGA@LB@ \|\|:\| \|\|\|\|\| \|\|\|\|\|\|: :\|\|\|:\|\|\|\|@LB@ UGUA GUCUU CUGCCAU UCAAUUACU@LB@3'-UUU...UUGUC A UACUUA UAUUUAUU ACUAUCG-5'@LB@ \| \|@LB@ 48 7@LB@@LB@@LB@Energy -11.19 kcal/mol@LB@Hybridization Energy -23.1983 kcal/mol@LB@Unfolding Energy - mRNA 5.6913 kcal/mol@LB@Unfolding Energy - sRNA 6.31565 kcal/mol@LB@@LB@Position - mRNA 245 -- 274@LB@Position - sRNA 8 -- 47@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 8 -- 14@LB@
12	0.000426	0.1165	b2331	smrB	-16.71	0.002472	163 -- 205	16 -- 57	putative DNA endonuclease		_____________162____________________________________________206\n_____________\|______________________________________________\|\n5'-UCA...GAGUC________UGUA___AAA________A______A____________AAAGA...GAC-3'\n______________GGGUAAAC____GGA___UUAGAAAU_GA_GAC___GAUAAAUGAA\n______________\|\|\|\|\|\|\|\|____:\|\|___\|:\|\|\|\|\|\|_:\|_\|\|\|___:\|\|\|\|\|\|:\|\|\n______________CCCAUUUG____UCU___AGUCUUUA_UU_CUG___UUAUUUAUUU\n3'-UUU...UUAUC_______________GUA________C__A___CCA__________CAAUU...UCG-5'\n_____________\|______________________________________________\|\n_____________58_____________________________________________15\n	199 -- 205	16 -- 22	-27.8577 kcal/mol	4.82255 kcal/mol	6.32306 kcal/mol	162 206@LB@ \| \|@LB@5'-UCA...GAGUC UGUA AAA A A AAAGA...GAC-3'@LB@ GGGUAAAC GGA UUAGAAAU GA GAC GAUAAAUGAA@LB@ \|\|\|\|\|\|\|\| :\|\| \|:\|\|\|\|\|\| :\| \|\|\| :\|\|\|\|\|\|:\|\|@LB@ CCCAUUUG UCU AGUCUUUA UU CUG UUAUUUAUUU@LB@3'-UUU...UUAUC GUA C A CCA CAAUU...UCG-5'@LB@ \| \|@LB@ 58 15@LB@@LB@@LB@Energy -16.71 kcal/mol@LB@Hybridization Energy -27.8577 kcal/mol@LB@Unfolding Energy - mRNA 4.82255 kcal/mol@LB@Unfolding Energy - sRNA 6.32306 kcal/mol@LB@@LB@Position - mRNA 163 -- 205@LB@Position - sRNA 16 -- 57@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 16 -- 22@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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