Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
38	0.003831	0.3287	b3739	atpI	-13.67	0.015109	253 -- 271	1 -- 16	ATP synthase membrane-bound accessory factor	_____________252_________________272\n_____________\|___________________\|\n5'-GAA...CGUUC_____CUG___________AAGUG...ACC-3'\n______________AGUUA___GUGGUGAUAGC\n______________\|\|\|\|\|___:\|\|:\|\|\|\|\|\|\|\n______________UCAAU___UACUACUAUCG\n3'-UUU...UUAUU___________________-5'\n_____________\|__________________\|\n_____________17_________________1\n	265 -- 271	1 -- 7	-18.239 kcal/mol	4.52285 kcal/mol	0.0492804 kcal/mol	252 272@LB@ \| \|@LB@5'-GAA...CGUUC CUG AAGUG...ACC-3'@LB@ AGUUA GUGGUGAUAGC@LB@ \|\|\|\|\| :\|\|:\|\|\|\|\|\|\|@LB@ UCAAU UACUACUAUCG@LB@3'-UUU...UUAUU -5'@LB@ \| \|@LB@ 17 1@LB@@LB@@LB@Energy -13.67 kcal/mol@LB@Hybridization Energy -18.239 kcal/mol@LB@Unfolding Energy - mRNA 4.52285 kcal/mol@LB@Unfolding Energy - sRNA 0.0492804 kcal/mol@LB@@LB@Position - mRNA 253 -- 271@LB@Position - sRNA 1 -- 16@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 1 -- 7@LB@
104	0.01633	0.5059	b3057	bacA	-10.04	0.095309	188 -- 194	54 -- 60	undecaprenyl pyrophosphate phosphatase	_____________187_____195\n_____________\|_______\|\n5'-UUC...UAAUU_______UUAUU...UUG-3'\n______________UAGGGGU\n______________\|\|\|\|\|\|\|\n______________AUCCCCA\n3'-UUU...ACUUU_______UUUGU...UCG-5'\n_____________\|_______\|\n_____________61______53\n	188 -- 194	54 -- 60	-11.2865 kcal/mol	1.18126 kcal/mol	0.0661814 kcal/mol	187 195@LB@ \| \|@LB@5'-UUC...UAAUU UUAUU...UUG-3'@LB@ UAGGGGU@LB@ \|\|\|\|\|\|\|@LB@ AUCCCCA@LB@3'-UUU...ACUUU UUUGU...UCG-5'@LB@ \| \|@LB@ 61 53@LB@@LB@@LB@Energy -10.04 kcal/mol@LB@Hybridization Energy -11.2865 kcal/mol@LB@Unfolding Energy - mRNA 1.18126 kcal/mol@LB@Unfolding Energy - sRNA 0.0661814 kcal/mol@LB@@LB@Position - mRNA 188 -- 194@LB@Position - sRNA 54 -- 60@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 54 -- 60@LB@
192	0.03268	0.5507	b3532	bcsB	-10.41	0.080313	186 -- 199	1 -- 12	regulator of cellulose synthase cyclic di-GMP binding	_____________185____________200\n_____________\|______________\|\n5'-AAG...UCAAC__________AC__GAUGA...UCA-3'\n______________AAUGAUGAUA__GC\n______________\|\|\|\|\|\|\|\|\|\|__\|\|\n______________UUACUACUAU__CG\n3'-UUU...UUCAA______________-5'\n_____________\|_____________\|\n_____________13____________1\n	189 -- 195	3 -- 9	-11.9692 kcal/mol	1.52301 kcal/mol	0.0394463 kcal/mol	185 200@LB@ \| \|@LB@5'-AAG...UCAAC AC GAUGA...UCA-3'@LB@ AAUGAUGAUA GC@LB@ \|\|\|\|\|\|\|\|\|\| \|\|@LB@ UUACUACUAU CG@LB@3'-UUU...UUCAA -5'@LB@ \| \|@LB@ 13 1@LB@@LB@@LB@Energy -10.41 kcal/mol@LB@Hybridization Energy -11.9692 kcal/mol@LB@Unfolding Energy - mRNA 1.52301 kcal/mol@LB@Unfolding Energy - sRNA 0.0394463 kcal/mol@LB@@LB@Position - mRNA 186 -- 199@LB@Position - sRNA 1 -- 12@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 3 -- 9@LB@
45	0.005107	0.3601	b0435	bolA	-10.01	0.096403	160 -- 209	3 -- 59	stationary-phase morphogene transcriptional repressor for mreB; also regulator for dacA dacC and ampC	_____________159________________________________________________________210\n_____________\|__________________________________________________________\|\n5'-UGA...UUUAA____A___________C____CGU______U___AGG______UUUC___________CGUGA...CCG-3'\n______________AGGG_UGGAU_GACAU_UCAG_______UG_CGG____AGAUA______AUGAUGAUA\n______________\|\|\|\|_\|::\|:_\|\|\|\|\|_\|\|\|\|_______\|\|_\|\|\|____\|:\|\|\|______\|\|\|\|\|\|\|\|\|\n______________UCCC_AUUUG_CUGUA_AGUC_______AC_GCC____UUUAU______UACUACUAU\n3'-UUU...CUUUA____C_____U__________UUUACUU__U___AUUA_____UUCAAU_________CG-5'\n_____________\|__________________________________________________________\|\n_____________60_________________________________________________________2\n	203 -- 209	3 -- 9	-31.3101 kcal/mol	14.9582 kcal/mol	6.33762 kcal/mol	159 210@LB@ \| \|@LB@5'-UGA...UUUAA A C CGU U AGG UUUC CGUGA...CCG-3'@LB@ AGGG UGGAU GACAU UCAG UG CGG AGAUA AUGAUGAUA@LB@ \|\|\|\| \|::\|: \|\|\|\|\| \|\|\|\| \|\| \|\|\| \|:\|\|\| \|\|\|\|\|\|\|\|\|@LB@ UCCC AUUUG CUGUA AGUC AC GCC UUUAU UACUACUAU@LB@3'-UUU...CUUUA C U UUUACUU U AUUA UUCAAU CG-5'@LB@ \| \|@LB@ 60 2@LB@@LB@@LB@Energy -10.01 kcal/mol@LB@Hybridization Energy -31.3101 kcal/mol@LB@Unfolding Energy - mRNA 14.9582 kcal/mol@LB@Unfolding Energy - sRNA 6.33762 kcal/mol@LB@@LB@Position - mRNA 160 -- 209@LB@Position - sRNA 3 -- 59@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 3 -- 9@LB@
134	0.02184	0.526	b0034	caiF	-11.03	0.059446	251 -- 259	1 -- 9	cai operon transcriptional activator	_____________250_______260\n_____________\|_________\|\n5'-UGA...GAAUG_________UGAAA...AUU-3'\n______________GAUGAUGGC\n______________\|\|\|\|\|\|:\|\|\n______________CUACUAUCG\n3'-UUU...AAUUA_________-5'\n_____________\|________\|\n_____________10_______1\n	253 -- 259	1 -- 7	-13.9707 kcal/mol	2.91573 kcal/mol	0.0200155 kcal/mol	250 260@LB@ \| \|@LB@5'-UGA...GAAUG UGAAA...AUU-3'@LB@ GAUGAUGGC@LB@ \|\|\|\|\|\|:\|\|@LB@ CUACUAUCG@LB@3'-UUU...AAUUA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -13.9707 kcal/mol@LB@Unfolding Energy - mRNA 2.91573 kcal/mol@LB@Unfolding Energy - sRNA 0.0200155 kcal/mol@LB@@LB@Position - mRNA 251 -- 259@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 1 -- 7@LB@
123	0.01951	0.5155	b0032	carA	-10.10	0.092857	258 -- 264	55 -- 61	carbamoyl phosphate synthetase small subunit glutamine amidotransferase	_____________257_____265\n_____________\|_______\|\n5'-UUG...GGGCC_______CAACA...AUA-3'\n______________AUAGGGG\n______________\|\|\|\|\|\|\|\n______________UAUCCCC\n3'-UUU...AACUU_______AUUUG...UCG-5'\n_____________\|_______\|\n_____________62______54\n	258 -- 264	55 -- 61	-10.8319 kcal/mol	0.710423 kcal/mol	0.0258473 kcal/mol	257 265@LB@ \| \|@LB@5'-UUG...GGGCC CAACA...AUA-3'@LB@ AUAGGGG@LB@ \|\|\|\|\|\|\|@LB@ UAUCCCC@LB@3'-UUU...AACUU AUUUG...UCG-5'@LB@ \| \|@LB@ 62 54@LB@@LB@@LB@Energy -10.10 kcal/mol@LB@Hybridization Energy -10.8319 kcal/mol@LB@Unfolding Energy - mRNA 0.710423 kcal/mol@LB@Unfolding Energy - sRNA 0.0258473 kcal/mol@LB@@LB@Position - mRNA 258 -- 264@LB@Position - sRNA 55 -- 61@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 55 -- 61@LB@
183	0.03025	0.5427	b0999	cbpM	-7.72	0.255487	289 -- 296	53 -- 60	modulator of CbpA co-chaperone	_____________288______297\n_____________\|________\|\n5'-AUC...CGGUU________GUUG-3'\n______________UGGGGGUG\n______________\|:\|\|\|\|\|:\n______________AUCCCCAU\n3'-UUU...ACUUU________UUGUC...UCG-5'\n_____________\|________\|\n_____________61_______52\n	290 -- 296	53 -- 59	-12.779 kcal/mol	4.99203 kcal/mol	0.0710089 kcal/mol	288 297@LB@ \| \|@LB@5'-AUC...CGGUU GUUG-3'@LB@ UGGGGGUG@LB@ \|:\|\|\|\|\|:@LB@ AUCCCCAU@LB@3'-UUU...ACUUU UUGUC...UCG-5'@LB@ \| \|@LB@ 61 52@LB@@LB@@LB@Energy -7.72 kcal/mol@LB@Hybridization Energy -12.779 kcal/mol@LB@Unfolding Energy - mRNA 4.99203 kcal/mol@LB@Unfolding Energy - sRNA 0.0710089 kcal/mol@LB@@LB@Position - mRNA 289 -- 296@LB@Position - sRNA 53 -- 60@LB@Position Seed - mRNA 290 -- 296@LB@Position Seed - sRNA 53 -- 59@LB@
94	0.01566	0.5059	b3918	cdh	-10.38	0.081442	225 -- 238	1 -- 14	CDP-diacylglycerol phosphotidylhydrolase	_____________224____________239\n_____________\|______________\|\n5'-AUU...UUUUU______________UAUCG...CAU-3'\n______________UUGGUGAUGAUAGU\n______________\|\|::\|\|\|\|\|\|\|\|\|:\n______________AAUUACUACUAUCG\n3'-UUU...AUUUC______________-5'\n_____________\|_____________\|\n_____________15____________1\n	232 -- 238	1 -- 7	-16.1883 kcal/mol	5.76383 kcal/mol	0.0473494 kcal/mol	224 239@LB@ \| \|@LB@5'-AUU...UUUUU UAUCG...CAU-3'@LB@ UUGGUGAUGAUAGU@LB@ \|\|::\|\|\|\|\|\|\|\|\|:@LB@ AAUUACUACUAUCG@LB@3'-UUU...AUUUC -5'@LB@ \| \|@LB@ 15 1@LB@@LB@@LB@Energy -10.38 kcal/mol@LB@Hybridization Energy -16.1883 kcal/mol@LB@Unfolding Energy - mRNA 5.76383 kcal/mol@LB@Unfolding Energy - sRNA 0.0473494 kcal/mol@LB@@LB@Position - mRNA 225 -- 238@LB@Position - sRNA 1 -- 14@LB@Position Seed - mRNA 232 -- 238@LB@Position Seed - sRNA 1 -- 7@LB@
136	0.02238	0.526	b1883	cheB	-10.46	0.078180	185 -- 206	1 -- 23	fused chemotaxis regulator: protein-glutamate methylesterase in two-component regulatory system with CheA	_____________184______________________207\n_____________\|________________________\|\n5'-GGG...UGCGC_________A____C_________AAAAU...CGA-3'\n______________UAAGUAAGG_UUAA_GAUGA__GC\n______________\|\|\|:\|\|\|:\|_\|\|\|\|_\|\|\|\|\|__\|\|\n______________AUUUAUUUC_AAUU_CUACU__CG\n3'-UUU...CCAUU______________A_____AU__-5'\n_____________\|_______________________\|\n_____________24______________________1\n	187 -- 193	15 -- 21	-15.1436 kcal/mol	4.57462 kcal/mol	0.105066 kcal/mol	184 207@LB@ \| \|@LB@5'-GGG...UGCGC A C AAAAU...CGA-3'@LB@ UAAGUAAGG UUAA GAUGA GC@LB@ \|\|\|:\|\|\|:\| \|\|\|\| \|\|\|\|\| \|\|@LB@ AUUUAUUUC AAUU CUACU CG@LB@3'-UUU...CCAUU A AU -5'@LB@ \| \|@LB@ 24 1@LB@@LB@@LB@Energy -10.46 kcal/mol@LB@Hybridization Energy -15.1436 kcal/mol@LB@Unfolding Energy - mRNA 4.57462 kcal/mol@LB@Unfolding Energy - sRNA 0.105066 kcal/mol@LB@@LB@Position - mRNA 185 -- 206@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 15 -- 21@LB@
99	0.01587	0.5059	b2155	cirA	-12.92	0.022646	275 -- 290	1 -- 16	colicin IA outer membrane receptor and translocator; ferric iron-catecholate transporter	_____________274______________291\n_____________\|________________\|\n5'-GGA...UUAGC___C____________GAAAC...UGG-3'\n______________GGU_GAUGAUGAUGGC\n______________:\|\|_:\|\|\|\|\|\|\|\|:\|\|\n______________UCA_UUACUACUAUCG\n3'-UUU...UUAUU___A____________-5'\n_____________\|_______________\|\n_____________17______________1\n	284 -- 290	1 -- 7	-19.8516 kcal/mol	6.87927 kcal/mol	0.0492804 kcal/mol	274 291@LB@ \| \|@LB@5'-GGA...UUAGC C GAAAC...UGG-3'@LB@ GGU GAUGAUGAUGGC@LB@ :\|\| :\|\|\|\|\|\|\|\|:\|\|@LB@ UCA UUACUACUAUCG@LB@3'-UUU...UUAUU A -5'@LB@ \| \|@LB@ 17 1@LB@@LB@@LB@Energy -12.92 kcal/mol@LB@Hybridization Energy -19.8516 kcal/mol@LB@Unfolding Energy - mRNA 6.87927 kcal/mol@LB@Unfolding Energy - sRNA 0.0492804 kcal/mol@LB@@LB@Position - mRNA 275 -- 290@LB@Position - sRNA 1 -- 16@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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