Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
53	0.005067	0.3217	b3666	uhpT	-10.07	0.266829	134 -- 177	167 -- 215	hexose phosphate transporter	_____________133_______________________________________________________178\n_____________\|_________________________________________________________\|\n5'-GCC...UGGAC____UCU____CC______________________UA________AUGCC_______GGUAU...UGG-3'\n______________GCUA___CAGG__UGAUUUGCU_GC___UGAUUUU____CAAUGC_____UCACGCA\n______________:\|:\|___\|\|\|\|__\|:\|\|:\|\|\|\|_\|\|___\|\|\|\|\|\|\|____\|\|\|\|:\|_____\|\|\|\|:\|\|\n______________UGGU___GUCC__AUUAGACGA_CG___ACUAAAA____GUUAUG_____AGUGUGU\n____3'-UUUUUUU____C______AU_________C__CCC_______UGUG______AGUC________ACUAC...UAG-5'\n_____________\|_________________________________________________________\|\n_____________216_______________________________________________________166\n	171 -- 177	167 -- 173	-37.7334 kcal/mol	11.6521 kcal/mol	18.4949 kcal/mol	133 178@LB@ \| \|@LB@5'-GCC...UGGAC UCU CC UA AUGCC GGUAU...UGG-3'@LB@ GCUA CAGG UGAUUUGCU GC UGAUUUU CAAUGC UCACGCA@LB@ :\|:\| \|\|\|\| \|:\|\|:\|\|\|\| \|\| \|\|\|\|\|\|\| \|\|\|\|:\| \|\|\|\|:\|\|@LB@ UGGU GUCC AUUAGACGA CG ACUAAAA GUUAUG AGUGUGU@LB@ 3'-UUUUUUU C AU C CCC UGUG AGUC ACUAC...UAG-5'@LB@ \| \|@LB@ 216 166@LB@@LB@@LB@Energy -10.07 kcal/mol@LB@Hybridization Energy -37.7334 kcal/mol@LB@Unfolding Energy - mRNA 11.6521 kcal/mol@LB@Unfolding Energy - sRNA 18.4949 kcal/mol@LB@@LB@Position - mRNA 134 -- 177@LB@Position - sRNA 167 -- 215@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 167 -- 173@LB@
131	0.01426	0.3575	b0048	folA	-12.08	0.107221	135 -- 211	115 -- 187	dihydrofolate reductase	_____________134_________212\n_____________\|___________\|\n5'-UCA...UUGUU___________GUAAU...GCA-3'\n______________UUUGUUUCAUU\n______________:\|\|\|:\|\|\|\|\|:\n______________GAACGAAGUAG\n3'-UUU...UGGGG___________-5'\n_____________\|__________\|\n_____________188________115\n	56 -- 62	1 -- 7	-10.5455 kcal/mol	1.7602 kcal/mol	1.03329 kcal/mol	134 212@LB@ \| \|@LB@5'-UCA...UUGUU GUAAU...GCA-3'@LB@ UUUGUUUCAUU@LB@ :\|\|\|:\|\|\|\|\|:@LB@ GAACGAAGUAG@LB@3'-UUU...UGGGG -5'@LB@ \| \|@LB@ 188 115@LB@@LB@@LB@Energy -12.08 kcal/mol@LB@Hybridization Energy -10.5455 kcal/mol@LB@Unfolding Energy - mRNA 1.7602 kcal/mol@LB@Unfolding Energy - sRNA 1.03329 kcal/mol@LB@@LB@Position - mRNA 135 -- 211@LB@Position - sRNA 115 -- 187@LB@Position Seed - mRNA 56 -- 62@LB@Position Seed - sRNA 1 -- 7@LB@
186	0.02152	0.3833	b3581	sgbH	-10.17	0.255482	135 -- 158	123 -- 153	3-keto-L-gulonate 6-phosphate decarboxylase	_____________134________159\n_____________\|__________\|\n5'-UUU...UAAGG__________UAUGA...AAG-3'\n______________AG_CACACAU\n______________\|\|_\|\|\|\|\|\|\|\n______________UC_GUGUGUA\n3'-UUU...AUGAG__A_______CUACG...UAG-5'\n_____________\|__________\|\n_____________154________122\n	193 -- 199	166 -- 172	-9.15702 kcal/mol	1.98532 kcal/mol	0.591348 kcal/mol	134 159@LB@ \| \|@LB@5'-UUU...UAAGG UAUGA...AAG-3'@LB@ AG CACACAU@LB@ \|\| \|\|\|\|\|\|\|@LB@ UC GUGUGUA@LB@3'-UUU...AUGAG A CUACG...UAG-5'@LB@ \| \|@LB@ 154 122@LB@@LB@@LB@Energy -10.17 kcal/mol@LB@Hybridization Energy -9.15702 kcal/mol@LB@Unfolding Energy - mRNA 1.98532 kcal/mol@LB@Unfolding Energy - sRNA 0.591348 kcal/mol@LB@@LB@Position - mRNA 135 -- 158@LB@Position - sRNA 123 -- 153@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 166 -- 172@LB@
57	0.00565	0.3247	b2081	yegQ	-17.30	0.009213	136 -- 160	1 -- 21	putative peptidase	_____________135_______________________161\n_____________\|_________________________\|\n5'-AUU...AAGUG_______U_____AACC________AGGUG...GUU-3'\n______________GGGGCAC_CCCCU____GCUUCAUC\n______________\|\|\|\|\|\|\|_\|\|\|\|\|____\|\|\|\|\|\|\|\|\n______________CCCCGUG_GGGGA____CGAAGUAG\n3'-UUU...GCGUA_____________A___________-5'\n_____________\|________________________\|\n_____________22_______________________1\n	154 -- 160	1 -- 7	-36.7075 kcal/mol	11.7869 kcal/mol	10.2895 kcal/mol	135 161@LB@ \| \|@LB@5'-AUU...AAGUG U AACC AGGUG...GUU-3'@LB@ GGGGCAC CCCCU GCUUCAUC@LB@ \|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\|\|@LB@ CCCCGUG GGGGA CGAAGUAG@LB@3'-UUU...GCGUA A -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -17.30 kcal/mol@LB@Hybridization Energy -36.7075 kcal/mol@LB@Unfolding Energy - mRNA 11.7869 kcal/mol@LB@Unfolding Energy - sRNA 10.2895 kcal/mol@LB@@LB@Position - mRNA 136 -- 160@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 1 -- 7@LB@
33	0.002591	0.2642	b0331	prpB	-22.44	0.000909	137 -- 159	1 -- 22	2-methylisocitrate lyase	_____________136__________________160\n_____________\|____________________\|\n5'-GUC...AUUGU_____A______________UUUAU...UGG-3'\n______________GGCAC_CCCCUUGCUUUGUC\n______________\|\|\|\|\|_\|\|\|\|\|\|\|\|\|\|::\|\|\n______________CCGUG_GGGGAACGAAGUAG\n3'-UUU...GUACC____________________-5'\n_____________\|___________________\|\n_____________23__________________1\n	153 -- 159	1 -- 7	-33.844 kcal/mol	4.2272 kcal/mol	8.43224 kcal/mol	136 160@LB@ \| \|@LB@5'-GUC...AUUGU A UUUAU...UGG-3'@LB@ GGCAC CCCCUUGCUUUGUC@LB@ \|\|\|\|\| \|\|\|\|\|\|\|\|\|\|::\|\|@LB@ CCGUG GGGGAACGAAGUAG@LB@3'-UUU...GUACC -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -22.44 kcal/mol@LB@Hybridization Energy -33.844 kcal/mol@LB@Unfolding Energy - mRNA 4.2272 kcal/mol@LB@Unfolding Energy - sRNA 8.43224 kcal/mol@LB@@LB@Position - mRNA 137 -- 159@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 153 -- 159@LB@Position Seed - sRNA 1 -- 7@LB@
142	0.01524	0.3575	b0611	rna	-12.80	0.076457	138 -- 176	173 -- 202	ribonuclease I	_____________137_____________________________________177\n_____________\|_______________________________________\|\n5'-UAC...CCCAC________CUUUUUUA______A___GA___________GUAUG...AUU-3'\n______________CUGGCGGG________UGAUUU_AUA__UAGUAUUCAGU\n______________\|\|\|\|\|\|\|\|________\|\|\|\|\|\|_\|:\|__:\|:\|\|:\|\|\|\|\|\n______________GACCGCCC________ACUAAA_UGU__GUUAUGAGUCA\n3'-UUU...UAGAC______________________A___G____________GUGUG...UAG-5'\n_____________\|_______________________________________\|\n_____________203_____________________________________172\n	170 -- 176	173 -- 179	-31.7889 kcal/mol	9.30484 kcal/mol	14.6886 kcal/mol	137 177@LB@ \| \|@LB@5'-UAC...CCCAC CUUUUUUA A GA GUAUG...AUU-3'@LB@ CUGGCGGG UGAUUU AUA UAGUAUUCAGU@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|\| \|:\| :\|:\|\|:\|\|\|\|\|@LB@ GACCGCCC ACUAAA UGU GUUAUGAGUCA@LB@3'-UUU...UAGAC A G GUGUG...UAG-5'@LB@ \| \|@LB@ 203 172@LB@@LB@@LB@Energy -12.80 kcal/mol@LB@Hybridization Energy -31.7889 kcal/mol@LB@Unfolding Energy - mRNA 9.30484 kcal/mol@LB@Unfolding Energy - sRNA 14.6886 kcal/mol@LB@@LB@Position - mRNA 138 -- 176@LB@Position - sRNA 173 -- 202@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 173 -- 179@LB@
42	0.003683	0.2912	b2221	atoD	-14.87	0.028783	141 -- 155	6 -- 19	acetyl-CoA:acetoacetyl-CoA transferase alpha subunit	_____________140_____________156\n_____________\|_______________\|\n5'-UAU...AUUCU_____U_________AUUGC...CCC-3'\n______________GGCAC_CCCCUUGCU\n______________\|\|\|\|\|_\|\|\|\|\|\|\|\|\|\n______________CCGUG_GGGGAACGA\n3'-UUU...GUACC_______________AGUAG-5'\n_____________\|_______________\|\n_____________20______________5\n	149 -- 155	6 -- 12	-27.0081 kcal/mol	4.79018 kcal/mol	8.30673 kcal/mol	140 156@LB@ \| \|@LB@5'-UAU...AUUCU U AUUGC...CCC-3'@LB@ GGCAC CCCCUUGCU@LB@ \|\|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ CCGUG GGGGAACGA@LB@3'-UUU...GUACC AGUAG-5'@LB@ \| \|@LB@ 20 5@LB@@LB@@LB@Energy -14.87 kcal/mol@LB@Hybridization Energy -27.0081 kcal/mol@LB@Unfolding Energy - mRNA 4.79018 kcal/mol@LB@Unfolding Energy - sRNA 8.30673 kcal/mol@LB@@LB@Position - mRNA 141 -- 155@LB@Position - sRNA 6 -- 19@LB@Position Seed - mRNA 149 -- 155@LB@Position Seed - sRNA 6 -- 12@LB@
184	0.02128	0.3833	b2831	mutH	-12.07	0.107608	142 -- 230	114 -- 189	methyl-directed mismatch repair protein	_____________141______________231\n_____________\|________________\|\n5'-UUC...CUGCG________________AUUGC...CGG-3'\n______________AAUAUUCGG_CACAUA\n______________\|\|\|\|:\|\|:\|_\|\|\|\|:\|\n______________UUAUGAGUC_GUGUGU\n3'-UUU...UGUGG_________A______ACUAC...UAG-5'\n_____________\|________________\|\n_____________190______________113\n	154 -- 160	174 -- 180	-15.1193 kcal/mol	2.199 kcal/mol	0.596263 kcal/mol	141 231@LB@ \| \|@LB@5'-UUC...CUGCG AUUGC...CGG-3'@LB@ AAUAUUCGG CACAUA@LB@ \|\|\|\|:\|\|:\| \|\|\|\|:\|@LB@ UUAUGAGUC GUGUGU@LB@3'-UUU...UGUGG A ACUAC...UAG-5'@LB@ \| \|@LB@ 190 113@LB@@LB@@LB@Energy -12.07 kcal/mol@LB@Hybridization Energy -15.1193 kcal/mol@LB@Unfolding Energy - mRNA 2.199 kcal/mol@LB@Unfolding Energy - sRNA 0.596263 kcal/mol@LB@@LB@Position - mRNA 142 -- 230@LB@Position - sRNA 114 -- 189@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 174 -- 180@LB@
65	0.006332	0.3261	b3857	mobA	-11.59	0.134663	143 -- 181	126 -- 173	molybdopterin-guanine dinucleotide synthase	_____________142________________________________________________________182\n_____________\|__________________________________________________________\|\n5'-UCC...AUAGU_______C_____U_U___GA___A__C______AG______________A_______AAUAA...AAC-3'\n______________GAUGCUC__UUAC_C_UGA__CAG_CA_GUUAGC____________GGUC_AUCCCAC\n______________:\|\|:\|\|\|__\|:\|\|_\|_\|\|\|__\|\|\|_\|\|_\|\|:\|:\|____________\|\|\|\|_\|\|\|\|\|\|\|\n______________UUAUGAG__AGUG_G_ACU__GUC_GU_CAGUUG____________CCAG_UAGGGUG\n3'-UUU...UGUGG_______UC____U_U___AC___C__U______AAAGUCUUAACG____________ACGUA...UAG-5'\n_____________\|__________________________________________________________\|\n_____________174________________________________________________________125\n	175 -- 181	126 -- 132	-38.7055 kcal/mol	10.57 kcal/mol	15.6637 kcal/mol	142 182@LB@ \| \|@LB@5'-UCC...AUAGU C U U GA A C AG A AAUAA...AAC-3'@LB@ GAUGCUC UUAC C UGA CAG CA GUUAGC GGUC AUCCCAC@LB@ :\|\|:\|\|\| \|:\|\| \| \|\|\| \|\|\| \|\| \|\|:\|:\| \|\|\|\| \|\|\|\|\|\|\|@LB@ UUAUGAG AGUG G ACU GUC GU CAGUUG CCAG UAGGGUG@LB@3'-UUU...UGUGG UC U U AC C U AAAGUCUUAACG ACGUA...UAG-5'@LB@ \| \|@LB@ 174 125@LB@@LB@@LB@Energy -11.59 kcal/mol@LB@Hybridization Energy -38.7055 kcal/mol@LB@Unfolding Energy - mRNA 10.57 kcal/mol@LB@Unfolding Energy - sRNA 15.6637 kcal/mol@LB@@LB@Position - mRNA 143 -- 181@LB@Position - sRNA 126 -- 173@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 126 -- 132@LB@
31	0.002426	0.2633	b2593	yfiH	-12.28	0.097562	144 -- 222	126 -- 203	UPF0124 family protein	_____________143___________________________________________________________________________223\n_____________\|_____________________________________________________________________________\|\n5'-CCA...AUUGA______GC______________AAGA_____AG_____________G____GG___U____AA__U_A_________AGUGG...CAC-3'\n______________GGUGAU___GCGCCGAU_UUCG____ACAUA__GAUG_____AAGU_GACU__UUA_GAGU__GC_G_UUGUCCCGC\n______________\|\|\|\|\|\|___:\|:\|\|:\|\|_:\|\|:____\|\|\|:\|__\|\|\|\|_____\|\|\|\|_:\|\|\|__\|:\|_\|\|:\|__\|\|_\|_\|::\|\|\|\|:\|\n______________CCACUA___UGUGGUUA_GAGU____UGUGU__CUAC_____UUCA_UUGA__AGU_CUUA__CG_C_AGUAGGGUG\n3'-UUU...ACCGC______AAA________U____CAG______A_____GUCCG____G____A_________A___C___________ACGUA...UAG-5'\n_____________\|_____________________________________________________________________________\|\n_____________204___________________________________________________________________________125\n	216 -- 222	126 -- 132	-51.0225 kcal/mol	17.9985 kcal/mol	20.5417 kcal/mol	143 223@LB@ \| \|@LB@5'-CCA...AUUGA GC AAGA AG G GG U AA U A AGUGG...CAC-3'@LB@ GGUGAU GCGCCGAU UUCG ACAUA GAUG AAGU GACU UUA GAGU GC G UUGUCCCGC@LB@ \|\|\|\|\|\| :\|:\|\|:\|\| :\|\|: \|\|\|:\| \|\|\|\| \|\|\|\| :\|\|\| \|:\| \|\|:\| \|\| \| \|::\|\|\|\|:\|@LB@ CCACUA UGUGGUUA GAGU UGUGU CUAC UUCA UUGA AGU CUUA CG C AGUAGGGUG@LB@3'-UUU...ACCGC AAA U CAG A GUCCG G A A C ACGUA...UAG-5'@LB@ \| \|@LB@ 204 125@LB@@LB@@LB@Energy -12.28 kcal/mol@LB@Hybridization Energy -51.0225 kcal/mol@LB@Unfolding Energy - mRNA 17.9985 kcal/mol@LB@Unfolding Energy - sRNA 20.5417 kcal/mol@LB@@LB@Position - mRNA 144 -- 222@LB@Position - sRNA 126 -- 203@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 126 -- 132@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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