Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
19	0.001698	0.2633	b2575	trmN	-12.50	0.088044	81 -- 92	14 -- 25	tRNA1(Val) (adenine(37)-N6)-methyltransferase	_____________80___________93\n_____________\|____________\|\n5'-ACC...UACAU__G_________GUAAU...GUA-3'\n______________CC_UGCUUCGUC\n______________\|\|_\|\|\|\|\|\|:\|\|\n______________GG_ACGAAGUAG\n3'-UUU...GUGGG__A_________-5'\n_____________\|___________\|\n_____________26__________14\n	222 -- 228	1 -- 7	-15.2724 kcal/mol	3.783 kcal/mol	1.04836 kcal/mol	80 93@LB@ \| \|@LB@5'-ACC...UACAU G GUAAU...GUA-3'@LB@ CC UGCUUCGUC@LB@ \|\| \|\|\|\|\|\|:\|\|@LB@ GG ACGAAGUAG@LB@3'-UUU...GUGGG A -5'@LB@ \| \|@LB@ 26 14@LB@@LB@@LB@Energy -12.50 kcal/mol@LB@Hybridization Energy -15.2724 kcal/mol@LB@Unfolding Energy - mRNA 3.783 kcal/mol@LB@Unfolding Energy - sRNA 1.04836 kcal/mol@LB@@LB@Position - mRNA 81 -- 92@LB@Position - sRNA 14 -- 25@LB@Position Seed - mRNA 222 -- 228@LB@Position Seed - sRNA 1 -- 7@LB@
44	0.003817	0.292	b0967	rlmI	-10.41	0.230051	81 -- 118	128 -- 161	23S rRNA m(5)C1962 methyltransferase SAM-dependent	_____________80_________________________________119\n_____________\|__________________________________\|\n5'-CGU...UGAAC____C___AAACC_______UUAU_CA_______GACAU...AAG-3'\n______________UCGC_CGU_____CAGGCAA____G__GACUUUC\n______________\|\|:\|_\|:\|_____\|\|\|\|\|\|\|____\|__:\|\|\|\|\|\|\n______________AGUG_GUA_____GUCCGUU____C__UUGAAAG\n3'-UUU...GAGUC____U___CUAC_____________AG_______UCUUA...UAG-5'\n_____________\|__________________________________\|\n_____________162________________________________127\n	95 -- 101	145 -- 151	-21.0542 kcal/mol	5.99356 kcal/mol	7.97203 kcal/mol	80 119@LB@ \| \|@LB@5'-CGU...UGAAC C AAACC UUAU CA GACAU...AAG-3'@LB@ UCGC CGU CAGGCAA G GACUUUC@LB@ \|\|:\| \|:\| \|\|\|\|\|\|\| \| :\|\|\|\|\|\|@LB@ AGUG GUA GUCCGUU C UUGAAAG@LB@3'-UUU...GAGUC U CUAC AG UCUUA...UAG-5'@LB@ \| \|@LB@ 162 127@LB@@LB@@LB@Energy -10.41 kcal/mol@LB@Hybridization Energy -21.0542 kcal/mol@LB@Unfolding Energy - mRNA 5.99356 kcal/mol@LB@Unfolding Energy - sRNA 7.97203 kcal/mol@LB@@LB@Position - mRNA 81 -- 118@LB@Position - sRNA 128 -- 161@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 145 -- 151@LB@
89	0.009985	0.3575	b0477	gsk	-9.51	0.338446	88 -- 115	123 -- 150	inosine/guanosine kinase	_____________87_____________116\n_____________\|______________\|\n5'-UUU...UCGCC_________AC___CCGUA...CUG-3'\n______________GCUCAGUUA__CAC\n______________:\|\|\|\|\|\|:\|__\|\|\|\n______________UGAGUCAGU__GUG\n3'-UUU...GGUUA______________UACUA...UAG-5'\n_____________\|______________\|\n_____________151____________122\n	175 -- 181	171 -- 177	-13.3754 kcal/mol	2.68541 kcal/mol	0.283892 kcal/mol	87 116@LB@ \| \|@LB@5'-UUU...UCGCC AC CCGUA...CUG-3'@LB@ GCUCAGUUA CAC@LB@ :\|\|\|\|\|\|:\| \|\|\|@LB@ UGAGUCAGU GUG@LB@3'-UUU...GGUUA UACUA...UAG-5'@LB@ \| \|@LB@ 151 122@LB@@LB@@LB@Energy -9.51 kcal/mol@LB@Hybridization Energy -13.3754 kcal/mol@LB@Unfolding Energy - mRNA 2.68541 kcal/mol@LB@Unfolding Energy - sRNA 0.283892 kcal/mol@LB@@LB@Position - mRNA 88 -- 115@LB@Position - sRNA 123 -- 150@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 171 -- 177@LB@
63	0.006154	0.3261	b3750	rbsC	-15.65	0.019915	89 -- 120	139 -- 174	D-ribose ABC transporter permease	_____________88___________________________________121\n_____________\|____________________________________\|\n5'-GAU...UCAUC___________A_______UC_____GG________UACUC...UCU-3'\n______________GUCAUGCAUGA___GGGCA__UCAGC____GGAAUU\n______________\|\|\|\|::\|\|\|\|\|___:\|\|\|\|__\|\|\|:\|____:\|\|\|\|\|\n______________CAGUGUGUACU___UCCGU__AGUUG____UCUUAA\n3'-UUU...UGAGU___________ACG_____UC_____AAAG______CGCCA...UAG-5'\n_____________\|____________________________________\|\n_____________175__________________________________138\n	91 -- 97	166 -- 172	-33.4294 kcal/mol	9.19626 kcal/mol	8.8011 kcal/mol	88 121@LB@ \| \|@LB@5'-GAU...UCAUC A UC GG UACUC...UCU-3'@LB@ GUCAUGCAUGA GGGCA UCAGC GGAAUU@LB@ \|\|\|\|::\|\|\|\|\| :\|\|\|\| \|\|\|:\| :\|\|\|\|\|@LB@ CAGUGUGUACU UCCGU AGUUG UCUUAA@LB@3'-UUU...UGAGU ACG UC AAAG CGCCA...UAG-5'@LB@ \| \|@LB@ 175 138@LB@@LB@@LB@Energy -15.65 kcal/mol@LB@Hybridization Energy -33.4294 kcal/mol@LB@Unfolding Energy - mRNA 9.19626 kcal/mol@LB@Unfolding Energy - sRNA 8.8011 kcal/mol@LB@@LB@Position - mRNA 89 -- 120@LB@Position - sRNA 139 -- 174@LB@Position Seed - mRNA 91 -- 97@LB@Position Seed - sRNA 166 -- 172@LB@
107	0.01169	0.3575	b3858	yihD	-12.50	0.087903	89 -- 104	63 -- 77	DUF1040 protein YihD	_____________88______________________________105\n_____________\|_______________________________\|\n5'-AUA...UUUAC___CU___________GCC____AUC_____CAUCA...CGA-3'\n______________CGC__GCCAGCACAAC___UGUU___GUCGU\n______________\|\|\|__:\|\|\|\|\|\|\|\|\|\|___:\|:\|___\|\|\|\|:\n______________GCG__UGGUCGUGUUG___GCGA___CAGCG\n3'-UUU...UGGUU___U____________AA_____________CCAUU...UAG-5'\n_____________\|_______________________________\|\n_____________78______________________________62\n	98 -- 104	63 -- 69	-31.4348 kcal/mol	4.67185 kcal/mol	17.7932 kcal/mol	88 105@LB@ \| \|@LB@5'-AUA...UUUAC CU GCC AUC CAUCA...CGA-3'@LB@ CGC GCCAGCACAAC UGUU GUCGU@LB@ \|\|\| :\|\|\|\|\|\|\|\|\|\| :\|:\| \|\|\|\|:@LB@ GCG UGGUCGUGUUG GCGA CAGCG@LB@3'-UUU...UGGUU U AA CCAUU...UAG-5'@LB@ \| \|@LB@ 78 62@LB@@LB@@LB@Energy -12.50 kcal/mol@LB@Hybridization Energy -31.4348 kcal/mol@LB@Unfolding Energy - mRNA 4.67185 kcal/mol@LB@Unfolding Energy - sRNA 17.7932 kcal/mol@LB@@LB@Position - mRNA 89 -- 104@LB@Position - sRNA 63 -- 77@LB@Position Seed - mRNA 98 -- 104@LB@Position Seed - sRNA 63 -- 69@LB@
82	0.008839	0.3575	b3680	yidL	-8.33	0.532337	91 -- 113	142 -- 164	AraC family putative transcriptional regulator	_____________90________________114\n_____________\|_________________\|\n5'-AAC...UGCGU________GCAG_____UUCUU...UGA-3'\n______________CGGGUCAG____UCAGA\n______________\|::\|\|\|\|:____\|\|\|\|\|\n______________GUUCAGUU____AGUCU\n3'-UUU...CGUCC________GAA______UAACG...UAG-5'\n_____________\|_________________\|\n_____________165_______________141\n	97 -- 103	151 -- 157	-14.3717 kcal/mol	4.36777 kcal/mol	4.37859 kcal/mol	90 114@LB@ \| \|@LB@5'-AAC...UGCGU GCAG UUCUU...UGA-3'@LB@ CGGGUCAG UCAGA@LB@ \|::\|\|\|\|: \|\|\|\|\|@LB@ GUUCAGUU AGUCU@LB@3'-UUU...CGUCC GAA UAACG...UAG-5'@LB@ \| \|@LB@ 165 141@LB@@LB@@LB@Energy -8.33 kcal/mol@LB@Hybridization Energy -14.3717 kcal/mol@LB@Unfolding Energy - mRNA 4.36777 kcal/mol@LB@Unfolding Energy - sRNA 4.37859 kcal/mol@LB@@LB@Position - mRNA 91 -- 113@LB@Position - sRNA 142 -- 164@LB@Position Seed - mRNA 97 -- 103@LB@Position Seed - sRNA 151 -- 157@LB@
92	0.01029	0.3575	b3360	pabA	-10.28	0.244097	93 -- 100	16 -- 23	aminodeoxychorismate synthase subunit II	_____________92__________101\n_____________\|___________\|\n5'-GCG...ACCUC___________UUGUG...CGU-3'\n______________UACCAGUACUU\n______________:\|\|\|\|:\|\|\|\|:\n______________GUGGUUAUGAG\n3'-UUU...AAAAU___________UCAGU...UAG-5'\n_____________\|___________\|\n_____________24__________15\n	250 -- 256	176 -- 182	-13.2399 kcal/mol	3.06354 kcal/mol	3.38772 kcal/mol	92 101@LB@ \| \|@LB@5'-GCG...ACCUC UUGUG...CGU-3'@LB@ UACCAGUACUU@LB@ :\|\|\|\|:\|\|\|\|:@LB@ GUGGUUAUGAG@LB@3'-UUU...AAAAU UCAGU...UAG-5'@LB@ \| \|@LB@ 24 15@LB@@LB@@LB@Energy -10.28 kcal/mol@LB@Hybridization Energy -13.2399 kcal/mol@LB@Unfolding Energy - mRNA 3.06354 kcal/mol@LB@Unfolding Energy - sRNA 3.38772 kcal/mol@LB@@LB@Position - mRNA 93 -- 100@LB@Position - sRNA 16 -- 23@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 176 -- 182@LB@
156	0.01739	0.3749	b4332	yjiJ	-7.83	0.625728	95 -- 115	80 -- 101	DUF1228 family putative inner membrane MFS superfamily transporter	_____________94_______116\n_____________\|________\|\n5'-AAC...GGUUU________ACAUC...UUU-3'\n______________GUCGCAUA\n______________\|\|\|:\|\|:\|\n______________CAGUGUGU\n3'-UUU...UGAGU________ACUAC...UAG-5'\n_____________\|________\|\n_____________102______79\n	23 -- 29	167 -- 173	-9.78459 kcal/mol	1.62979 kcal/mol	0.409692 kcal/mol	94 116@LB@ \| \|@LB@5'-AAC...GGUUU ACAUC...UUU-3'@LB@ GUCGCAUA@LB@ \|\|\|:\|\|:\|@LB@ CAGUGUGU@LB@3'-UUU...UGAGU ACUAC...UAG-5'@LB@ \| \|@LB@ 102 79@LB@@LB@@LB@Energy -7.83 kcal/mol@LB@Hybridization Energy -9.78459 kcal/mol@LB@Unfolding Energy - mRNA 1.62979 kcal/mol@LB@Unfolding Energy - sRNA 0.409692 kcal/mol@LB@@LB@Position - mRNA 95 -- 115@LB@Position - sRNA 80 -- 101@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 167 -- 173@LB@
172	0.01969	0.3812	b0213	yafS	-9.47	0.344290	95 -- 113	1 -- 19	putative S-adenosyl-L-methionine-dependent methyltransferase	_____________94________114\n_____________\|_________\|\n5'-UGG...UCAAA_________CUCCU...UCA-3'\n______________CUGUCGUGG\n______________\|\|\|\|\|\|:\|\|\n______________GACAGCGCC\n3'-UUU...GAAGC_________AUUUU...UAG-5'\n_____________\|_________\|\n_____________20________0\n	228 -- 234	50 -- 56	-16.2917 kcal/mol	2.78861 kcal/mol	4.02 kcal/mol	94 114@LB@ \| \|@LB@5'-UGG...UCAAA CUCCU...UCA-3'@LB@ CUGUCGUGG@LB@ \|\|\|\|\|\|:\|\|@LB@ GACAGCGCC@LB@3'-UUU...GAAGC AUUUU...UAG-5'@LB@ \| \|@LB@ 20 0@LB@@LB@@LB@Energy -9.47 kcal/mol@LB@Hybridization Energy -16.2917 kcal/mol@LB@Unfolding Energy - mRNA 2.78861 kcal/mol@LB@Unfolding Energy - sRNA 4.02 kcal/mol@LB@@LB@Position - mRNA 95 -- 113@LB@Position - sRNA 1 -- 19@LB@Position Seed - mRNA 228 -- 234@LB@Position Seed - sRNA 50 -- 56@LB@
111	0.0122	0.3575	b1813	nudL	-9.44	0.347588	99 -- 131	174 -- 206	putative CoA pyrophosphohydrolase weak 3-phosphohydroxypyruvate phosphatase	_____________98________________________________132\n_____________\|_________________________________\|\n5'-CCU...UCUGC_____G_____UG__A__GU________GC___GAAGA...CUG-3'\n______________UCUGC_GGCGG__GA_UU__CGCCGAUA__CAG\n______________\|\|\|\|\|_\|\|\|\|\|__\|\|_\|\|__\|:\|\|:\|\|\|__\|\|\|\n______________AGACG_CCGCC__CU_AA__GUGGUUAU__GUC\n3'-UUU...AUAUU_____A_____CA__A__AU________GA___AGUGU...UAG-5'\n_____________\|_________________________________\|\n_____________207_______________________________173\n	120 -- 126	179 -- 185	-36.2385 kcal/mol	15.3331 kcal/mol	14.8345 kcal/mol	98 132@LB@ \| \|@LB@5'-CCU...UCUGC G UG A GU GC GAAGA...CUG-3'@LB@ UCUGC GGCGG GA UU CGCCGAUA CAG@LB@ \|\|\|\|\| \|\|\|\|\| \|\| \|\| \|:\|\|:\|\|\| \|\|\|@LB@ AGACG CCGCC CU AA GUGGUUAU GUC@LB@3'-UUU...AUAUU A CA A AU GA AGUGU...UAG-5'@LB@ \| \|@LB@ 207 173@LB@@LB@@LB@Energy -9.44 kcal/mol@LB@Hybridization Energy -36.2385 kcal/mol@LB@Unfolding Energy - mRNA 15.3331 kcal/mol@LB@Unfolding Energy - sRNA 14.8345 kcal/mol@LB@@LB@Position - mRNA 99 -- 131@LB@Position - sRNA 174 -- 206@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 179 -- 185@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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