Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
70	0.00686	0.3298	b0761	modE	-10.05	0.269682	248 -- 271	57 -- 77	transcriptional repressor for the molybdenum transport operon modABC	_____________247______272\n_____________\|________\|\n5'-ACC...GAAAA________CUUUU...CCA-3'\n______________GCACUCCC\n______________\|\|\|\|:\|\|\|\n______________CGUGGGGG\n3'-UUU...UACCC________AACGA...UAG-5'\n_____________\|________\|\n_____________78_______56\n	126 -- 132	11 -- 17	-14.7879 kcal/mol	0.633098 kcal/mol	5.13224 kcal/mol	247 272@LB@ \| \|@LB@5'-ACC...GAAAA CUUUU...CCA-3'@LB@ GCACUCCC@LB@ \|\|\|\|:\|\|\|@LB@ CGUGGGGG@LB@3'-UUU...UACCC AACGA...UAG-5'@LB@ \| \|@LB@ 78 56@LB@@LB@@LB@Energy -10.05 kcal/mol@LB@Hybridization Energy -14.7879 kcal/mol@LB@Unfolding Energy - mRNA 0.633098 kcal/mol@LB@Unfolding Energy - sRNA 5.13224 kcal/mol@LB@@LB@Position - mRNA 248 -- 271@LB@Position - sRNA 57 -- 77@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 11 -- 17@LB@
129	0.01414	0.3575	b3579	yiaO	-11.03	0.174508	249 -- 273	50 -- 76	2 3-diketo-L-gulonate-binding periplasmic protein	_____________248_________________________274\n_____________\|___________________________\|\n5'-ACU...CGUCC____________C__UA__________UUGUU...AAA-3'\n______________UGGUUUUAUAUU_AU__UUAGUCGUAU\n______________\|\|:\|\|\|\|\|:\|::_\|\|__\|:\|\|\|\|::\|:\n______________ACUAAAAUGUGG_UA__AGUCAGUGUG\n3'-UUU...CGCCC____________U__UG__________UACUA...UAG-5'\n_____________\|___________________________\|\n_____________77__________________________49\n	132 -- 138	168 -- 174	-19.0823 kcal/mol	5.4875 kcal/mol	5.03533 kcal/mol	248 274@LB@ \| \|@LB@5'-ACU...CGUCC C UA UUGUU...AAA-3'@LB@ UGGUUUUAUAUU AU UUAGUCGUAU@LB@ \|\|:\|\|\|\|\|:\|:: \|\| \|:\|\|\|\|::\|:@LB@ ACUAAAAUGUGG UA AGUCAGUGUG@LB@3'-UUU...CGCCC U UG UACUA...UAG-5'@LB@ \| \|@LB@ 77 49@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -19.0823 kcal/mol@LB@Unfolding Energy - mRNA 5.4875 kcal/mol@LB@Unfolding Energy - sRNA 5.03533 kcal/mol@LB@@LB@Position - mRNA 249 -- 273@LB@Position - sRNA 50 -- 76@LB@Position Seed - mRNA 132 -- 138@LB@Position Seed - sRNA 168 -- 174@LB@
11	0.0006963	0.1842	b1739	osmE	-13.14	0.065080	250 -- 260	194 -- 204	osmotically-inducible lipoprotein	_____________249_________261\n_____________\|___________\|\n5'-GAA...AACCA___________UGUAC...AGC-3'\n______________UGCUGGCGGGU\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________ACGACCGCCCA\n3'-UUU...AUUAG___________CUAAA...UAG-5'\n_____________\|___________\|\n_____________205_________193\n	254 -- 260	194 -- 200	-22.742 kcal/mol	5.67651 kcal/mol	8.00138 kcal/mol	249 261@LB@ \| \|@LB@5'-GAA...AACCA UGUAC...AGC-3'@LB@ UGCUGGCGGGU@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ ACGACCGCCCA@LB@3'-UUU...AUUAG CUAAA...UAG-5'@LB@ \| \|@LB@ 205 193@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -22.742 kcal/mol@LB@Unfolding Energy - mRNA 5.67651 kcal/mol@LB@Unfolding Energy - sRNA 8.00138 kcal/mol@LB@@LB@Position - mRNA 250 -- 260@LB@Position - sRNA 194 -- 204@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 194 -- 200@LB@
26	0.002141	0.2633	b0183	rnhB	-16.17	0.015603	251 -- 292	123 -- 175	ribonuclease HII degrades RNA of DNA-RNA hybrids	______________251_______________________________________________________293\n______________\|_________________________________________________________\|\n___________5'-____________________________AG_G_______AAGAAUUAU__________AGUGU...UCC-3'\n______________AAUCUGCUGGCGGG___________CAG__A_UUAGUCA_________UGCAGG_AAG\n______________\|\|\|\|\|\|\|\|\|\|\|\|\|\|___________\|\|:__\|_\|:\|\|\|\|\|_________\|\|\|\|\|\|_\|\|\|\n______________UUAGACGACCGCCC___________GUU__U_AGUCAGU_________ACGUCC_UUC\n3'-UUU...CCAUA______________ACUAAAAUGUG___A__G_______GUGUACU________G___AGUUG...UAG-5'\n_____________\|__________________________________________________________\|\n_____________176________________________________________________________122\n	22 -- 28	171 -- 177	-46.3072 kcal/mol	12.4456 kcal/mol	20.994 kcal/mol	251 293@LB@ \| \|@LB@ 5'- AG G AAGAAUUAU AGUGU...UCC-3'@LB@ AAUCUGCUGGCGGG CAG A UUAGUCA UGCAGG AAG@LB@ \|\|\|\|\|\|\|\|\|\|\|\|\|\| \|\|: \| \|:\|\|\|\|\| \|\|\|\|\|\| \|\|\|@LB@ UUAGACGACCGCCC GUU U AGUCAGU ACGUCC UUC@LB@3'-UUU...CCAUA ACUAAAAUGUG A G GUGUACU G AGUUG...UAG-5'@LB@ \| \|@LB@ 176 122@LB@@LB@@LB@Energy -16.17 kcal/mol@LB@Hybridization Energy -46.3072 kcal/mol@LB@Unfolding Energy - mRNA 12.4456 kcal/mol@LB@Unfolding Energy - sRNA 20.994 kcal/mol@LB@@LB@Position - mRNA 251 -- 292@LB@Position - sRNA 123 -- 175@LB@Position Seed - mRNA 22 -- 28@LB@Position Seed - sRNA 171 -- 177@LB@
32	0.002513	0.2642	b3456	livM	-11.89	0.117100	251 -- 271	186 -- 206	branched-chain amino acid ABC transporter permease	_____________250___________________________272\n_____________\|_____________________________\|\n5'-GCC...CUGCU______GCCG___________UG______CCGGA...CCA-3'\n______________GGUGAU_____ACCGGUAUUC__GGUCGC\n______________\|\|\|\|\|\|_____\|\|\|::\|\|:\|\|__:\|\|\|:\|\n______________CCACUA_____UGGUUAUGAG__UCAGUG\n3'-UUU...ACCGC______AAAUG__________________UGUAC...UAG-5'\n_____________\|_____________________________\|\n_____________207___________________________185\n	169 -- 175	176 -- 182	-27.5836 kcal/mol	14.0763 kcal/mol	5.03772 kcal/mol	250 272@LB@ \| \|@LB@5'-GCC...CUGCU GCCG UG CCGGA...CCA-3'@LB@ GGUGAU ACCGGUAUUC GGUCGC@LB@ \|\|\|\|\|\| \|\|\|::\|\|:\|\| :\|\|\|:\|@LB@ CCACUA UGGUUAUGAG UCAGUG@LB@3'-UUU...ACCGC AAAUG UGUAC...UAG-5'@LB@ \| \|@LB@ 207 185@LB@@LB@@LB@Energy -11.89 kcal/mol@LB@Hybridization Energy -27.5836 kcal/mol@LB@Unfolding Energy - mRNA 14.0763 kcal/mol@LB@Unfolding Energy - sRNA 5.03772 kcal/mol@LB@@LB@Position - mRNA 251 -- 271@LB@Position - sRNA 186 -- 206@LB@Position Seed - mRNA 169 -- 175@LB@Position Seed - sRNA 176 -- 182@LB@
110	0.01217	0.3575	b1324	tpx	-10.54	0.217228	251 -- 258	126 -- 133	lipid hydroperoxide peroxidase	_____________250____________259\n_____________\|______________\|\n5'-GCG...ACUCC______________GUCUG...AAG-3'\n______________UUGUUGUCGCGGUG\n______________\|:\|:\|\|\|\|\|\|\|\|\|:\n______________AGCGACAGCGCCAU\n3'-UUU...GUUGA______________UUUAU...UAG-5'\n_____________\|______________\|\n_____________134____________125\n	99 -- 105	48 -- 54	-22.761 kcal/mol	7.03952 kcal/mol	5.88061 kcal/mol	250 259@LB@ \| \|@LB@5'-GCG...ACUCC GUCUG...AAG-3'@LB@ UUGUUGUCGCGGUG@LB@ \|:\|:\|\|\|\|\|\|\|\|\|:@LB@ AGCGACAGCGCCAU@LB@3'-UUU...GUUGA UUUAU...UAG-5'@LB@ \| \|@LB@ 134 125@LB@@LB@@LB@Energy -10.54 kcal/mol@LB@Hybridization Energy -22.761 kcal/mol@LB@Unfolding Energy - mRNA 7.03952 kcal/mol@LB@Unfolding Energy - sRNA 5.88061 kcal/mol@LB@@LB@Position - mRNA 251 -- 258@LB@Position - sRNA 126 -- 133@LB@Position Seed - mRNA 99 -- 105@LB@Position Seed - sRNA 48 -- 54@LB@
165	0.01884	0.3812	b3291	mscL	-11.12	0.167422	252 -- 267	193 -- 208	mechanosensitive channel protein high conductance	_____________251_____268\n_____________\|_______\|\n5'-UGU...UGUCG_______AGGGA...UCU-3'\n______________GCUUCAU\n______________\|\|\|\|\|\|\|\n______________CGAAGUA\n3'-UUU...GGGAA_______G-5'\n_____________\|_______\|\n_____________209_____192\n	182 -- 188	2 -- 8	-9.41561 kcal/mol	2.23742 kcal/mol	0.70263 kcal/mol	251 268@LB@ \| \|@LB@5'-UGU...UGUCG AGGGA...UCU-3'@LB@ GCUUCAU@LB@ \|\|\|\|\|\|\|@LB@ CGAAGUA@LB@3'-UUU...GGGAA G-5'@LB@ \| \|@LB@ 209 192@LB@@LB@@LB@Energy -11.12 kcal/mol@LB@Hybridization Energy -9.41561 kcal/mol@LB@Unfolding Energy - mRNA 2.23742 kcal/mol@LB@Unfolding Energy - sRNA 0.70263 kcal/mol@LB@@LB@Position - mRNA 252 -- 267@LB@Position - sRNA 193 -- 208@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 2 -- 8@LB@
28	0.002284	0.2633	b1114	mfd	-18.81	0.004608	253 -- 297	50 -- 95	transcription-repair coupling factor	_____________252______________298\n_____________\|________________\|\n5'-UUC...GCGUC___U____________CGGCG...AAA-3'\n______________UGC_GGGCGAGUUAAC\n______________\|\|\|_:\|\|\|:\|\|\|:\|\|\|\n______________ACG_UCCGUUCAGUUG\n3'-UUU...GUACU________________AAAGU...UAG-5'\n_____________\|________________\|\n_____________96_______________49\n	262 -- 268	149 -- 155	-20.6961 kcal/mol	4.60584 kcal/mol	7.12854 kcal/mol	252 298@LB@ \| \|@LB@5'-UUC...GCGUC U CGGCG...AAA-3'@LB@ UGC GGGCGAGUUAAC@LB@ \|\|\| :\|\|\|:\|\|\|:\|\|\|@LB@ ACG UCCGUUCAGUUG@LB@3'-UUU...GUACU AAAGU...UAG-5'@LB@ \| \|@LB@ 96 49@LB@@LB@@LB@Energy -18.81 kcal/mol@LB@Hybridization Energy -20.6961 kcal/mol@LB@Unfolding Energy - mRNA 4.60584 kcal/mol@LB@Unfolding Energy - sRNA 7.12854 kcal/mol@LB@@LB@Position - mRNA 253 -- 297@LB@Position - sRNA 50 -- 95@LB@Position Seed - mRNA 262 -- 268@LB@Position Seed - sRNA 149 -- 155@LB@
52	0.005023	0.3217	b0403	malZ	-9.64	0.320537	253 -- 286	155 -- 185	maltodextrin glucosidase	_____________252___________________________287\n_____________\|_____________________________\|\n5'-UUU...UCAAC_____________U____C__A_______AGACC...AGC-3'\n______________UGCUCA_UUACAC_GUGG_UG_CGGGCGA\n______________\|:\|\|\|\|_\|:\|\|\|\|_:\|\|:_\|\|_\|:\|\|\|:\|\n______________AUGAGU_AGUGUG_UACU_AC_GUCCGUU\n3'-UUU...UGGUU______C______________________CAGUU...UAG-5'\n_____________\|_____________________________\|\n_____________186___________________________154\n	280 -- 286	155 -- 161	-27.4446 kcal/mol	6.9929 kcal/mol	7.82707 kcal/mol	252 287@LB@ \| \|@LB@5'-UUU...UCAAC U C A AGACC...AGC-3'@LB@ UGCUCA UUACAC GUGG UG CGGGCGA@LB@ \|:\|\|\|\| \|:\|\|\|\| :\|\|: \|\| \|:\|\|\|:\|@LB@ AUGAGU AGUGUG UACU AC GUCCGUU@LB@3'-UUU...UGGUU C CAGUU...UAG-5'@LB@ \| \|@LB@ 186 154@LB@@LB@@LB@Energy -9.64 kcal/mol@LB@Hybridization Energy -27.4446 kcal/mol@LB@Unfolding Energy - mRNA 6.9929 kcal/mol@LB@Unfolding Energy - sRNA 7.82707 kcal/mol@LB@@LB@Position - mRNA 253 -- 286@LB@Position - sRNA 155 -- 185@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 155 -- 161@LB@
191	0.02237	0.3833	b1658	purR	-11.05	0.172432	254 -- 265	163 -- 174	transcriptional repressor hypoxanthine-binding	_____________253_______________266\n_____________\|_________________\|\n5'-UUU...CUACA___GU____________CAACA...GCA-3'\n______________ACU__GUCACACGUGAU\n______________\|\|\|__\|\|\|\|\|\|\|:\|\|\|\|\n______________UGA__CAGUGUGUACUA\n3'-UUU...GGUUA___GU____________CGUCC...UAG-5'\n_____________\|_________________\|\n_____________175_______________162\n	259 -- 265	163 -- 169	-20.3148 kcal/mol	3.57431 kcal/mol	4.30742 kcal/mol	253 266@LB@ \| \|@LB@5'-UUU...CUACA GU CAACA...GCA-3'@LB@ ACU GUCACACGUGAU@LB@ \|\|\| \|\|\|\|\|\|\|:\|\|\|\|@LB@ UGA CAGUGUGUACUA@LB@3'-UUU...GGUUA GU CGUCC...UAG-5'@LB@ \| \|@LB@ 175 162@LB@@LB@@LB@Energy -11.05 kcal/mol@LB@Hybridization Energy -20.3148 kcal/mol@LB@Unfolding Energy - mRNA 3.57431 kcal/mol@LB@Unfolding Energy - sRNA 4.30742 kcal/mol@LB@@LB@Position - mRNA 254 -- 265@LB@Position - sRNA 163 -- 174@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 163 -- 169@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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