Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
90	0.01008	0.3575	b3994	thiC	-11.53	0.138483	145 -- 158	1 -- 14	phosphomethylpyrimidine synthase	_____________144_________159\n_____________\|___________\|\n5'-AUG...CGUCU___________CGUCG...UUU-3'\n______________CUUGCUUCAUC\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________GAACGAAGUAG\n3'-UUU...UGGGG___________-5'\n_____________\|__________\|\n_____________15_________1\n	152 -- 158	1 -- 7	-17.5294 kcal/mol	1.22878 kcal/mol	1.03329 kcal/mol	144 159@LB@ \| \|@LB@5'-AUG...CGUCU CGUCG...UUU-3'@LB@ CUUGCUUCAUC@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ GAACGAAGUAG@LB@3'-UUU...UGGGG -5'@LB@ \| \|@LB@ 15 1@LB@@LB@@LB@Energy -11.53 kcal/mol@LB@Hybridization Energy -17.5294 kcal/mol@LB@Unfolding Energy - mRNA 1.22878 kcal/mol@LB@Unfolding Energy - sRNA 1.03329 kcal/mol@LB@@LB@Position - mRNA 145 -- 158@LB@Position - sRNA 1 -- 14@LB@Position Seed - mRNA 152 -- 158@LB@Position Seed - sRNA 1 -- 7@LB@
51	0.005002	0.3217	b0115	aceF	-12.52	0.087110	146 -- 171	149 -- 173	pyruvate dehydrogenase dihydrolipoyltransacetylase component E2	_____________145______________________________________172\n_____________\|________________________________________\|\n5'-GAU...GCAAU_____________________C_______AUA________CCGCG...CGC-3'\n______________CGCCAA_AUUCA_____ACAU_GAUGCAG___AAGUUAAC\n______________\|:\|\|\|\|_\|:\|\|\|_____\|\|\|\|_\|\|\|\|\|\|\|___\|\|\|\|:\|\|\|\n______________GUGGUU_UGAGU_____UGUA_CUACGUC___UUCAGUUG\n3'-UUU...AAAAU______A_____CAGUG____________CG_________AAAGU...UAG-5'\n_____________\|________________________________________\|\n_____________174______________________________________148\n	165 -- 171	149 -- 155	-31.6788 kcal/mol	5.24034 kcal/mol	12.6269 kcal/mol	145 172@LB@ \| \|@LB@5'-GAU...GCAAU C AUA CCGCG...CGC-3'@LB@ CGCCAA AUUCA ACAU GAUGCAG AAGUUAAC@LB@ \|:\|\|\|\| \|:\|\|\| \|\|\|\| \|\|\|\|\|\|\| \|\|\|\|:\|\|\|@LB@ GUGGUU UGAGU UGUA CUACGUC UUCAGUUG@LB@3'-UUU...AAAAU A CAGUG CG AAAGU...UAG-5'@LB@ \| \|@LB@ 174 148@LB@@LB@@LB@Energy -12.52 kcal/mol@LB@Hybridization Energy -31.6788 kcal/mol@LB@Unfolding Energy - mRNA 5.24034 kcal/mol@LB@Unfolding Energy - sRNA 12.6269 kcal/mol@LB@@LB@Position - mRNA 146 -- 171@LB@Position - sRNA 149 -- 173@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 149 -- 155@LB@
148	0.01584	0.3602	b1822	rlmA	-10.68	0.203557	148 -- 239	111 -- 186	23S rRNA m(1)G745 methyltransferase SAM-dependent	_____________147___________240\n_____________\|_____________\|\n5'-AAG...GCGUG_____C_______UUGCG...GGU-3'\n______________AUUCG_GUUACAC\n______________\|:\|\|:_\|\|:\|\|\|\|\n______________UGAGU_CAGUGUG\n3'-UUU...GGUUA_____________UACUA...UAG-5'\n_____________\|_____________\|\n_____________187___________110\n	163 -- 169	168 -- 174	-12.5288 kcal/mol	1.89227 kcal/mol	0.283892 kcal/mol	147 240@LB@ \| \|@LB@5'-AAG...GCGUG C UUGCG...GGU-3'@LB@ AUUCG GUUACAC@LB@ \|:\|\|: \|\|:\|\|\|\|@LB@ UGAGU CAGUGUG@LB@3'-UUU...GGUUA UACUA...UAG-5'@LB@ \| \|@LB@ 187 110@LB@@LB@@LB@Energy -10.68 kcal/mol@LB@Hybridization Energy -12.5288 kcal/mol@LB@Unfolding Energy - mRNA 1.89227 kcal/mol@LB@Unfolding Energy - sRNA 0.283892 kcal/mol@LB@@LB@Position - mRNA 148 -- 239@LB@Position - sRNA 111 -- 186@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 168 -- 174@LB@
18	0.001156	0.216	b3474	yhhT	-13.05	0.067927	149 -- 189	169 -- 204	UPF0118 family putative transporter	_____________148__________________________________________________________________190\n_____________\|____________________________________________________________________\|\n5'-GAC...GACGU____________UAAG____AGAAUAU________U_ACUCCCGC_____U______AAAA_______AGGAA...UCA-3'\n______________GAAAAUCAGCAG____AAUC_______UGCUGGCG_G________UACAC_UAAUAC____AGUCACA\n______________:\|\|\|\|:\|\|\|\|\|\|____\|\|\|\|_______\|\|\|\|\|\|\|\|_\|________\|\|\|\|\|_:\|\|\|\|\|____\|\|\|\|\|\|\|\n______________UUUUUGGUCGUC____UUAG_______ACGACCGC_C________AUGUG_GUUAUG____UCAGUGU\n________3'-UUU____________CAUA_____________________CACUAAA_____________AG_________GUACU...UAG-5'\n_____________\|____________________________________________________________________\|\n_____________205__________________________________________________________________168\n	183 -- 189	169 -- 175	-48.4478 kcal/mol	16.2259 kcal/mol	18.3074 kcal/mol	148 190@LB@ \| \|@LB@5'-GAC...GACGU UAAG AGAAUAU U ACUCCCGC U AAAA AGGAA...UCA-3'@LB@ GAAAAUCAGCAG AAUC UGCUGGCG G UACAC UAAUAC AGUCACA@LB@ :\|\|\|\|:\|\|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \| \|\|\|\|\| :\|\|\|\|\| \|\|\|\|\|\|\|@LB@ UUUUUGGUCGUC UUAG ACGACCGC C AUGUG GUUAUG UCAGUGU@LB@ 3'-UUU CAUA CACUAAA AG GUACU...UAG-5'@LB@ \| \|@LB@ 205 168@LB@@LB@@LB@Energy -13.05 kcal/mol@LB@Hybridization Energy -48.4478 kcal/mol@LB@Unfolding Energy - mRNA 16.2259 kcal/mol@LB@Unfolding Energy - sRNA 18.3074 kcal/mol@LB@@LB@Position - mRNA 149 -- 189@LB@Position - sRNA 169 -- 204@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 169 -- 175@LB@
167	0.01932	0.3812	b2912	fau	-13.57	0.053124	153 -- 189	109 -- 152	5-formyltetrahydrofolate cyclo-ligase family protein	_____________152__________190\n_____________\|____________\|\n5'-GAA...UGAGC____________GUCCG...UGG-3'\n______________AUGCUCGGUC_C\n______________\|\|:\|\|\|:\|\|\|_\|\n______________UAUGAGUCAG_G\n3'-UUU...GUGGU__________U_UGUAC...UAG-5'\n_____________\|____________\|\n_____________153__________108\n	31 -- 37	172 -- 178	-13.9133 kcal/mol	3.57919 kcal/mol	0.28482 kcal/mol	152 190@LB@ \| \|@LB@5'-GAA...UGAGC GUCCG...UGG-3'@LB@ AUGCUCGGUC C@LB@ \|\|:\|\|\|:\|\|\| \|@LB@ UAUGAGUCAG G@LB@3'-UUU...GUGGU U UGUAC...UAG-5'@LB@ \| \|@LB@ 153 108@LB@@LB@@LB@Energy -13.57 kcal/mol@LB@Hybridization Energy -13.9133 kcal/mol@LB@Unfolding Energy - mRNA 3.57919 kcal/mol@LB@Unfolding Energy - sRNA 0.28482 kcal/mol@LB@@LB@Position - mRNA 153 -- 189@LB@Position - sRNA 109 -- 152@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 172 -- 178@LB@
147	0.01564	0.358	b3153	yhbO	-15.63	0.020103	154 -- 186	72 -- 98	stress-resistance protein	_____________153_______________________________187\n_____________\|_________________________________\|\n5'-GUU...AUCCC__A________AAUU___CUCAU__________AACGC...UUA-3'\n______________CG_UUGUGGGA____CGC_____CCAAUGCAAC\n______________\|\|_\|\|\|\|\|\|\|\|____\|\|\|_____\|\|\|\|:\|\|\|\|\|\n______________GC_AACACCCU____GCG_____GGUUGCGUUG\n3'-UUU...AAUUA__________________AAUU___________GUCGU...UAG-5'\n_____________\|_________________________________\|\n_____________99________________________________71\n	180 -- 186	72 -- 78	-31.4938 kcal/mol	8.17407 kcal/mol	13.9341 kcal/mol	153 187@LB@ \| \|@LB@5'-GUU...AUCCC A AAUU CUCAU AACGC...UUA-3'@LB@ CG UUGUGGGA CGC CCAAUGCAAC@LB@ \|\| \|\|\|\|\|\|\|\| \|\|\| \|\|\|\|:\|\|\|\|\|@LB@ GC AACACCCU GCG GGUUGCGUUG@LB@3'-UUU...AAUUA AAUU GUCGU...UAG-5'@LB@ \| \|@LB@ 99 71@LB@@LB@@LB@Energy -15.63 kcal/mol@LB@Hybridization Energy -31.4938 kcal/mol@LB@Unfolding Energy - mRNA 8.17407 kcal/mol@LB@Unfolding Energy - sRNA 13.9341 kcal/mol@LB@@LB@Position - mRNA 154 -- 186@LB@Position - sRNA 72 -- 98@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 72 -- 78@LB@
71	0.00726	0.3408	b2944	yggI	-9.32	0.365111	156 -- 174	1 -- 18	Zn-dependent metalloprotease-related protein	_____________155_____________175\n_____________\|_______________\|\n5'-GAA...AGCCA________AG_____UUUUC...ACC-3'\n______________CCUCUUGC__UCAUC\n______________\|\|:\|\|\|\|\|__\|\|\|\|\|\n______________GGGGAACG__AGUAG\n3'-UUU...CCGUG________A______-5'\n_____________\|______________\|\n_____________19_____________1\n	161 -- 167	7 -- 13	-18.0684 kcal/mol	1.30619 kcal/mol	6.71812 kcal/mol	155 175@LB@ \| \|@LB@5'-GAA...AGCCA AG UUUUC...ACC-3'@LB@ CCUCUUGC UCAUC@LB@ \|\|:\|\|\|\|\| \|\|\|\|\|@LB@ GGGGAACG AGUAG@LB@3'-UUU...CCGUG A -5'@LB@ \| \|@LB@ 19 1@LB@@LB@@LB@Energy -9.32 kcal/mol@LB@Hybridization Energy -18.0684 kcal/mol@LB@Unfolding Energy - mRNA 1.30619 kcal/mol@LB@Unfolding Energy - sRNA 6.71812 kcal/mol@LB@@LB@Position - mRNA 156 -- 174@LB@Position - sRNA 1 -- 18@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 7 -- 13@LB@
119	0.013	0.3575	b0801	ybiC	-16.26	0.015008	158 -- 253	108 -- 187	putative dehydrogenase	_____________157____________________________________________________________________________________________254\n_____________\|______________________________________________________________________________________________\|\n5'-GCU...AUAAA_____AA__AA______ACAUAAA_____________CAU_____A__________G_UUUGAUGCUCAGAC_____________UA_______UGUAU...UUG-3'\n______________AAUAC__CA__CACAUA_______CAGG__AGUUAAC____GGAA__GUGGUCAUC_C______________GCUGCA_CAGUUU__UUCAGGC\n______________\|\|\|\|\|__\|\|__\|\|\|\|:\|_______\|\|\|\|__\|\|\|:\|\|\|____:\|\|\|__\|:\|\|\|\|\|\|\|_\|______________:\|\|\|\|\|_\|\|\|\|::__\|\|\|\|:\|\|\n______________UUAUG__GU__GUGUGU_______GUCC__UCAGUUG____UCUU__CGCCAGUAG_G______________UGACGU_GUCAGG__AAGUUCG\n3'-UUU...UGUGG_____A___CA______ACUAC______GU_______AAAG____AA_________G_____________________A_______________UACCA...UAG-5'\n_____________\|______________________________________________________________________________________________\|\n_____________188____________________________________________________________________________________________107\n	247 -- 253	108 -- 114	-56.5044 kcal/mol	18.8914 kcal/mol	26.5178 kcal/mol	157 254@LB@ \| \|@LB@5'-GCU...AUAAA AA AA ACAUAAA CAU A G UUUGAUGCUCAGAC UA UGUAU...UUG-3'@LB@ AAUAC CA CACAUA CAGG AGUUAAC GGAA GUGGUCAUC C GCUGCA CAGUUU UUCAGGC@LB@ \|\|\|\|\| \|\| \|\|\|\|:\| \|\|\|\| \|\|\|:\|\|\| :\|\|\| \|:\|\|\|\|\|\|\| \| :\|\|\|\|\| \|\|\|\|:: \|\|\|\|:\|\|@LB@ UUAUG GU GUGUGU GUCC UCAGUUG UCUU CGCCAGUAG G UGACGU GUCAGG AAGUUCG@LB@3'-UUU...UGUGG A CA ACUAC GU AAAG AA G A UACCA...UAG-5'@LB@ \| \|@LB@ 188 107@LB@@LB@@LB@Energy -16.26 kcal/mol@LB@Hybridization Energy -56.5044 kcal/mol@LB@Unfolding Energy - mRNA 18.8914 kcal/mol@LB@Unfolding Energy - sRNA 26.5178 kcal/mol@LB@@LB@Position - mRNA 158 -- 253@LB@Position - sRNA 108 -- 187@LB@Position Seed - mRNA 247 -- 253@LB@Position Seed - sRNA 108 -- 114@LB@
143	0.01531	0.3575	b1641	slyB	-10.83	0.190300	158 -- 167	83 -- 92	outer membrane lipoprotein	_____________157____________________________168\n_____________\|______________________________\|\n5'-CCG...UCACA_______U______UGAC____AGG_____UUGCG...AAG-3'\n______________UAUGCCA_AUACUU____CAUG___GAUGC\n______________\|\|::\|\|\|_\|\|\|\|\|:____\|\|::___\|\|\|\|\|\n______________AUGUGGU_UAUGAG____GUGU___CUACG\n3'-UUU...CUAAA______________UCA_____GUA_____UCCGU...UAG-5'\n_____________\|______________________________\|\n_____________93_____________________________82\n	135 -- 141	182 -- 188	-20.8318 kcal/mol	7.22081 kcal/mol	7.72227 kcal/mol	157 168@LB@ \| \|@LB@5'-CCG...UCACA U UGAC AGG UUGCG...AAG-3'@LB@ UAUGCCA AUACUU CAUG GAUGC@LB@ \|\|::\|\|\| \|\|\|\|\|: \|\|:: \|\|\|\|\|@LB@ AUGUGGU UAUGAG GUGU CUACG@LB@3'-UUU...CUAAA UCA GUA UCCGU...UAG-5'@LB@ \| \|@LB@ 93 82@LB@@LB@@LB@Energy -10.83 kcal/mol@LB@Hybridization Energy -20.8318 kcal/mol@LB@Unfolding Energy - mRNA 7.22081 kcal/mol@LB@Unfolding Energy - sRNA 7.72227 kcal/mol@LB@@LB@Position - mRNA 158 -- 167@LB@Position - sRNA 83 -- 92@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 182 -- 188@LB@
60	0.005924	0.3261	b4201	priB	-15.35	0.022961	159 -- 180	18 -- 40	primosomal protein N	_____________158_____________181\n_____________\|_______________\|\n5'-AGG...GAAAC_______C_______ACGAU...AUC-3'\n______________UACCUUC__GCUUCA\n______________:\|\|\|::\|__\|\|\|\|\|\|\n______________GUGGGGG__CGAAGU\n3'-UUU...ACCCC_______AA______AG-5'\n_____________\|_______________\|\n_____________41______________17\n	26 -- 32	11 -- 17	-15.0598 kcal/mol	3.0465 kcal/mol	7.09151 kcal/mol	158 181@LB@ \| \|@LB@5'-AGG...GAAAC C ACGAU...AUC-3'@LB@ UACCUUC GCUUCA@LB@ :\|\|\|::\| \|\|\|\|\|\|@LB@ GUGGGGG CGAAGU@LB@3'-UUU...ACCCC AA AG-5'@LB@ \| \|@LB@ 41 17@LB@@LB@@LB@Energy -15.35 kcal/mol@LB@Hybridization Energy -15.0598 kcal/mol@LB@Unfolding Energy - mRNA 3.0465 kcal/mol@LB@Unfolding Energy - sRNA 7.09151 kcal/mol@LB@@LB@Position - mRNA 159 -- 180@LB@Position - sRNA 18 -- 40@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 11 -- 17@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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