Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
63	0.003337	0.1851	b1453	ansP	-14.01	0.047364	263 -- 293	1 -- 34	L-asparagine transporter		_____________262_________________________________294\n_____________\|___________________________________\|\n5'-GUC...AAUGC________A__________C_________AUG___AAUCGCC-3'\n______________CCACGAAG_GGGG_UAUCA____AAAGCG___GGC\n______________\|\|\|\|:\|\|:_\|\|\|\|_:\|\|\|\|____\|\|\|\|\|:___\|\|\|\n______________GGUGUUUU_CCCC_GUAGU____UUUCGU___CCG\n3'-UUU...CCCGA________A____U_____UUCU______CA____-5'\n_____________\|__________________________________\|\n_____________35_________________________________1\n	263 -- 269	28 -- 34	-31.7064 kcal/mol	13.4305 kcal/mol	4.26884 kcal/mol	262 294@LB@ \| \|@LB@5'-GUC...AAUGC A C AUG AAUCGCC-3'@LB@ CCACGAAG GGGG UAUCA AAAGCG GGC@LB@ \|\|\|\|:\|\|: \|\|\|\| :\|\|\|\| \|\|\|\|\|: \|\|\|@LB@ GGUGUUUU CCCC GUAGU UUUCGU CCG@LB@3'-UUU...CCCGA A U UUCU CA -5'@LB@ \| \|@LB@ 35 1@LB@@LB@@LB@Energy -14.01 kcal/mol@LB@Hybridization Energy -31.7064 kcal/mol@LB@Unfolding Energy - mRNA 13.4305 kcal/mol@LB@Unfolding Energy - sRNA 4.26884 kcal/mol@LB@@LB@Position - mRNA 263 -- 293@LB@Position - sRNA 1 -- 34@LB@Position Seed - mRNA 263 -- 269@LB@Position Seed - sRNA 28 -- 34@LB@
158	0.01161	0.2504	b3907	rhaT	-15.90	0.016445	218 -- 234	1 -- 28	L-rhamnose:proton symporter		_____________217__________________________235\n_____________\|____________________________\|\n5'-AUG...AUUAC____________U_______________AUUUG...CAU-3'\n______________GAUGGGGAUAUU____________UGGC\n______________:\|\|\|\|\|\|\|:\|\|:____________\|\|\|\|\n______________UUACCCCUGUAG____________ACCG\n3'-UUU...GUGUU____________UUUCUUUUCGUC____-5'\n_____________\|___________________________\|\n_____________29__________________________1\n	223 -- 229	17 -- 23	-20.3321 kcal/mol	1.91011 kcal/mol	2.519 kcal/mol	217 235@LB@ \| \|@LB@5'-AUG...AUUAC U AUUUG...CAU-3'@LB@ GAUGGGGAUAUU UGGC@LB@ :\|\|\|\|\|\|\|:\|\|: \|\|\|\|@LB@ UUACCCCUGUAG ACCG@LB@3'-UUU...GUGUU UUUCUUUUCGUC -5'@LB@ \| \|@LB@ 29 1@LB@@LB@@LB@Energy -15.90 kcal/mol@LB@Hybridization Energy -20.3321 kcal/mol@LB@Unfolding Energy - mRNA 1.91011 kcal/mol@LB@Unfolding Energy - sRNA 2.519 kcal/mol@LB@@LB@Position - mRNA 218 -- 234@LB@Position - sRNA 1 -- 28@LB@Position Seed - mRNA 223 -- 229@LB@Position Seed - sRNA 17 -- 23@LB@
188	0.01388	0.2529	b4198	ulaF	-10.93	0.193396	24 -- 37	2 -- 17	L-ribulose 5-phosphate 4-epimerase		_____________23_______________38\n_____________\|________________\|\n5'-GUC...UUUGG______U_________AUUUC...GCG-3'\n______________CGAAGG___GUAGUGG\n______________\|:\|\|\|:___\|:\|\|\|\|\|\n______________GUUUCU___CGUCACC\n3'-UUU...CUGUA______UUU_______G-5'\n_____________\|________________\|\n_____________18_______________1\n	31 -- 37	2 -- 8	-15.3961 kcal/mol	3.02615 kcal/mol	1.43836 kcal/mol	23 38@LB@ \| \|@LB@5'-GUC...UUUGG U AUUUC...GCG-3'@LB@ CGAAGG GUAGUGG@LB@ \|:\|\|\|: \|:\|\|\|\|\|@LB@ GUUUCU CGUCACC@LB@3'-UUU...CUGUA UUU G-5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -10.93 kcal/mol@LB@Hybridization Energy -15.3961 kcal/mol@LB@Unfolding Energy - mRNA 3.02615 kcal/mol@LB@Unfolding Energy - sRNA 1.43836 kcal/mol@LB@@LB@Position - mRNA 24 -- 37@LB@Position - sRNA 2 -- 17@LB@Position Seed - mRNA 31 -- 37@LB@Position Seed - sRNA 2 -- 8@LB@
30	0.001442	0.1635	b3458	livK	-12.84	0.084462	180 -- 215	6 -- 33	leucine transporter subunit		_____________179___________________________________216\n_____________\|_____________________________________\|\n5'-UGU...AACAU______________UUUUGACU__G___C____U___AAAAC...CGA-3'\n______________CAC_GAAUGGGGAU________AU_AAA_GGAA_GCG\n______________\|\|\|_:\|\|\|\|\|\|\|\|:________\|\|_\|\|\|_\|:\|\|_\|\|:\n______________GUG_UUUACCCCUG________UA_UUU_CUUU_CGU\n3'-UUU...CCGAG___U____________________G________U___CACCG-5'\n_____________\|_____________________________________\|\n_____________34____________________________________5\n	186 -- 192	20 -- 26	-21.4141 kcal/mol	5.49822 kcal/mol	3.07787 kcal/mol	179 216@LB@ \| \|@LB@5'-UGU...AACAU UUUUGACU G C U AAAAC...CGA-3'@LB@ CAC GAAUGGGGAU AU AAA GGAA GCG@LB@ \|\|\| :\|\|\|\|\|\|\|\|: \|\| \|\|\| \|:\|\| \|\|:@LB@ GUG UUUACCCCUG UA UUU CUUU CGU@LB@3'-UUU...CCGAG U G U CACCG-5'@LB@ \| \|@LB@ 34 5@LB@@LB@@LB@Energy -12.84 kcal/mol@LB@Hybridization Energy -21.4141 kcal/mol@LB@Unfolding Energy - mRNA 5.49822 kcal/mol@LB@Unfolding Energy - sRNA 3.07787 kcal/mol@LB@@LB@Position - mRNA 180 -- 215@LB@Position - sRNA 6 -- 33@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 20 -- 26@LB@
69	0.003755	0.191	b0889	lrp	-14.02	0.046951	165 -- 212	1 -- 32	leucine-responsive global transcriptional regulator		_____________164______________________________________________213\n_____________\|________________________________________________\|\n5'-GUG...GUCAG______GUA____AGGAAUA________CAAUAAUAAU_____AUA__AAGAA...AGC-3'\n______________ACAGGA___GGGA_______CAGAGAGA__________GGUAG___GC\n______________\|\|\|::\|___\|\|\|\|_______\|\|:\|\|\|:\|__________:\|:\|\|___\|\|\n______________UGUUUU___CCCU_______GUUUCUUU__________UCGUC___CG\n3'-UUU...CGAGG______AC_____GUA___________________________AC___-5'\n_____________\|_______________________________________________\|\n_____________33______________________________________________1\n	186 -- 192	10 -- 16	-19.2298 kcal/mol	2.66644 kcal/mol	2.53929 kcal/mol	164 213@LB@ \| \|@LB@5'-GUG...GUCAG GUA AGGAAUA CAAUAAUAAU AUA AAGAA...AGC-3'@LB@ ACAGGA GGGA CAGAGAGA GGUAG GC@LB@ \|\|\|::\| \|\|\|\| \|\|:\|\|\|:\| :\|:\|\| \|\|@LB@ UGUUUU CCCU GUUUCUUU UCGUC CG@LB@3'-UUU...CGAGG AC GUA AC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -14.02 kcal/mol@LB@Hybridization Energy -19.2298 kcal/mol@LB@Unfolding Energy - mRNA 2.66644 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 165 -- 212@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 10 -- 16@LB@
139	0.01022	0.2504	b3163	nlpI	-13.31	0.067339	19 -- 42	4 -- 21	lipoprotein involved in osmotic sensitivity and filamentation		_____________18_______________________43\n_____________\|________________________\|\n5'-AGG...UCUGA___________GCGACU_______CUCAA...CAU-3'\n______________GCAUUAAAGAA______GAGCAGU\n______________:\|\|\|:\|\|\|\|\|\|______:\|\|\|\|\|\|\n______________UGUAGUUUCUU______UUCGUCA\n3'-UUU...ACCCC________________________CCG-5'\n_____________\|________________________\|\n_____________22_______________________3\n	36 -- 42	4 -- 10	-18.4935 kcal/mol	3.67449 kcal/mol	1.51 kcal/mol	18 43@LB@ \| \|@LB@5'-AGG...UCUGA GCGACU CUCAA...CAU-3'@LB@ GCAUUAAAGAA GAGCAGU@LB@ :\|\|\|:\|\|\|\|\|\| :\|\|\|\|\|\|@LB@ UGUAGUUUCUU UUCGUCA@LB@3'-UUU...ACCCC CCG-5'@LB@ \| \|@LB@ 22 3@LB@@LB@@LB@Energy -13.31 kcal/mol@LB@Hybridization Energy -18.4935 kcal/mol@LB@Unfolding Energy - mRNA 3.67449 kcal/mol@LB@Unfolding Energy - sRNA 1.51 kcal/mol@LB@@LB@Position - mRNA 19 -- 42@LB@Position - sRNA 4 -- 21@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 4 -- 10@LB@
163	0.01202	0.2504	b1482	osmC	-10.53	0.225653	203 -- 226	1 -- 22	lipoyl-dependent Cys-based peroxidase hydroperoxide resistance; salt-shock inducible membrane protein; peroxiredoxin		_____________202_______________________227\n_____________\|_________________________\|\n5'-UAG...ACAAU___A___CAU_________CA____ACACU...CGU-3'\n______________GAC_AUC___AAGAAAGGU___GGC\n______________\|\|\|_\|\|\|___\|\|\|\|\|\|:\|:___\|\|\|\n______________CUG_UAG___UUCUUUUCG___CCG\n3'-UUU...UACCC_______U___________UCA___-5'\n_____________\|________________________\|\n_____________23_______________________1\n	215 -- 221	7 -- 13	-17.1921 kcal/mol	5.11752 kcal/mol	1.54023 kcal/mol	202 227@LB@ \| \|@LB@5'-UAG...ACAAU A CAU CA ACACU...CGU-3'@LB@ GAC AUC AAGAAAGGU GGC@LB@ \|\|\| \|\|\| \|\|\|\|\|\|:\|: \|\|\|@LB@ CUG UAG UUCUUUUCG CCG@LB@3'-UUU...UACCC U UCA -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -10.53 kcal/mol@LB@Hybridization Energy -17.1921 kcal/mol@LB@Unfolding Energy - mRNA 5.11752 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 203 -- 226@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 7 -- 13@LB@
115	0.007886	0.2401	b2890	lysS	-11.64	0.144658	161 -- 186	1 -- 20	lysine tRNA synthetase constitutive	b4129	_____________160_________________________187\n_____________\|___________________________\|\n5'-AAA...CAAUU________CAAGUUU____________UAUGA...GGA-3'\n______________UAUCGAAG_______GAAAGCAG_GGU\n______________:\|\|\|:\|\|\|_______:\|\|\|\|\|\|\|_\|\|:\n______________GUAGUUUC_______UUUUCGUC_CCG\n3'-UUU...CCCCU_______________________A___-5'\n_____________\|__________________________\|\n_____________21_________________________1\n	177 -- 183	5 -- 11	-18.6981 kcal/mol	5.60124 kcal/mol	1.45855 kcal/mol	160 187@LB@ \| \|@LB@5'-AAA...CAAUU CAAGUUU UAUGA...GGA-3'@LB@ UAUCGAAG GAAAGCAG GGU@LB@ :\|\|\|:\|\|\| :\|\|\|\|\|\|\| \|\|:@LB@ GUAGUUUC UUUUCGUC CCG@LB@3'-UUU...CCCCU A -5'@LB@ \| \|@LB@ 21 1@LB@@LB@@LB@Energy -11.64 kcal/mol@LB@Hybridization Energy -18.6981 kcal/mol@LB@Unfolding Energy - mRNA 5.60124 kcal/mol@LB@Unfolding Energy - sRNA 1.45855 kcal/mol@LB@@LB@Position - mRNA 161 -- 186@LB@Position - sRNA 1 -- 20@LB@Position Seed - mRNA 177 -- 183@LB@Position Seed - sRNA 5 -- 11@LB@
28	0.001242	0.1512	b0879	macB	-8.34	0.466885	135 -- 159	1 -- 22	macrolide ABC transporter peremase/ATPase		_____________134_________________________160\n_____________\|___________________________\|\n5'-AAU...GUUGA__U_________GCUU___________GAUGA...CGG-3'\n______________GA_UGUCAAAGG____GAAGC_G_GGC\n______________\|\|_::\|\|\|\|\|\|:____:\|\|\|\|_\|_\|\|\|\n______________CU_GUAGUUUCU____UUUCG_C_CCG\n3'-UUU...UACCC_____________________U_A___-5'\n_____________\|__________________________\|\n_____________23_________________________1\n	140 -- 146	12 -- 18	-17.2972 kcal/mol	7.41924 kcal/mol	1.54023 kcal/mol	134 160@LB@ \| \|@LB@5'-AAU...GUUGA U GCUU GAUGA...CGG-3'@LB@ GA UGUCAAAGG GAAGC G GGC@LB@ \|\| ::\|\|\|\|\|\|: :\|\|\|\| \| \|\|\|@LB@ CU GUAGUUUCU UUUCG C CCG@LB@3'-UUU...UACCC U A -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -8.34 kcal/mol@LB@Hybridization Energy -17.2972 kcal/mol@LB@Unfolding Energy - mRNA 7.41924 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 135 -- 159@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 140 -- 146@LB@Position Seed - sRNA 12 -- 18@LB@
171	0.01235	0.2504	b1092	fabD	-9.75	0.300248	258 -- 270	47 -- 59	malonyl-CoA-[acyl-carrier-protein] transacylase		_____________257___________271\n_____________\|_____________\|\n5'-AAC...UGCUG_____U_______CGAGC...UUG-3'\n______________GCUGA_AUGGCGG\n______________:\|:\|\|_\|\|\|\|\|\|\|\n______________UGGCU_UACCGCC\n3'-UUU...GAACU_____U_______CCAAC...CCG-5'\n_____________\|_____________\|\n_____________60____________46\n	264 -- 270	47 -- 53	-17.9053 kcal/mol	4.19285 kcal/mol	3.96442 kcal/mol	257 271@LB@ \| \|@LB@5'-AAC...UGCUG U CGAGC...UUG-3'@LB@ GCUGA AUGGCGG@LB@ :\|:\|\| \|\|\|\|\|\|\|@LB@ UGGCU UACCGCC@LB@3'-UUU...GAACU U CCAAC...CCG-5'@LB@ \| \|@LB@ 60 46@LB@@LB@@LB@Energy -9.75 kcal/mol@LB@Hybridization Energy -17.9053 kcal/mol@LB@Unfolding Energy - mRNA 4.19285 kcal/mol@LB@Unfolding Energy - sRNA 3.96442 kcal/mol@LB@@LB@Position - mRNA 258 -- 270@LB@Position - sRNA 47 -- 59@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 47 -- 53@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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