Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
199	0.01447	0.2552	b2790	yqcA	-9.70	0.305430	160 -- 205	1 -- 31	short-chain flavodoxin FMN-binding		_____________159_____________________________________________206\n_____________\|_______________________________________________\|\n5'-CUA...CGGGU________A__G__AAGUUCCUAAUC________AAAC_________GGAAA...CAA-3'\n______________CAGGAUGG_GA_AU____________CAGGGAGA____GGUA_UGGC\n______________\|\|::\|\|\|\|_\|\|_\|\|____________\|\|::\|\|:\|____:\|:\|_\|\|\|\|\n______________GUUUUACC_CU_UA____________GUUUCUUU____UCGU_ACCG\n3'-UUU...GAGGU________C__G______________________________C____-5'\n_____________\|______________________________________________\|\n_____________32_____________________________________________1\n	187 -- 193	10 -- 16	-23.6635 kcal/mol	11.4257 kcal/mol	2.53929 kcal/mol	159 206@LB@ \| \|@LB@5'-CUA...CGGGU A G AAGUUCCUAAUC AAAC GGAAA...CAA-3'@LB@ CAGGAUGG GA AU CAGGGAGA GGUA UGGC@LB@ \|\|::\|\|\|\| \|\| \|\| \|\|::\|\|:\| :\|:\| \|\|\|\|@LB@ GUUUUACC CU UA GUUUCUUU UCGU ACCG@LB@3'-UUU...GAGGU C G C -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -9.70 kcal/mol@LB@Hybridization Energy -23.6635 kcal/mol@LB@Unfolding Energy - mRNA 11.4257 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 160 -- 205@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 10 -- 16@LB@
83	0.005101	0.2147	b2610	ffh	-14.32	0.040245	57 -- 74	4 -- 21	Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)		_____________56_________________75\n_____________\|__________________\|\n5'-AGG...GAGCA___AG_____________AUAGC...AAG-3'\n______________AUA__AAAGAGAAGCAGU\n______________\|:\|__\|\|\|\|\|:\|\|\|\|\|\|\|\n______________UGU__UUUCUUUUCGUCA\n3'-UUU...ACCCC___AG_____________CCG-5'\n_____________\|__________________\|\n_____________22_________________3\n	68 -- 74	4 -- 10	-18.2522 kcal/mol	2.42435 kcal/mol	1.51 kcal/mol	56 75@LB@ \| \|@LB@5'-AGG...GAGCA AG AUAGC...AAG-3'@LB@ AUA AAAGAGAAGCAGU@LB@ \|:\| \|\|\|\|\|:\|\|\|\|\|\|\|@LB@ UGU UUUCUUUUCGUCA@LB@3'-UUU...ACCCC AG CCG-5'@LB@ \| \|@LB@ 22 3@LB@@LB@@LB@Energy -14.32 kcal/mol@LB@Hybridization Energy -18.2522 kcal/mol@LB@Unfolding Energy - mRNA 2.42435 kcal/mol@LB@Unfolding Energy - sRNA 1.51 kcal/mol@LB@@LB@Position - mRNA 57 -- 74@LB@Position - sRNA 4 -- 21@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 4 -- 10@LB@
95	0.005813	0.2147	b3450	ugpC	-12.76	0.087557	84 -- 111	1 -- 34	sn-glycerol-3-phosphate ABC transporter ATPase		_____________83_________________________________112\n_____________\|__________________________________\|\n5'-GUG...AGGCA___________A________U_____________GAUGU...UCG-3'\n______________CCACGGAAUGG__AC_UCAG_GA____UGGUGGC\n______________\|\|\|\|::\|\|\|\|\|__\|\|_\|\|\|:_\|\|____::\|\|\|\|\|\n______________GGUGUUUUACC__UG_AGUU_CU____GUCACCG\n3'-UUU...CCCGA___________CC__U____U__UUUC_______-5'\n_____________\|_________________________________\|\n_____________35________________________________1\n	105 -- 111	1 -- 7	-27.9886 kcal/mol	10.9582 kcal/mol	4.26884 kcal/mol	83 112@LB@ \| \|@LB@5'-GUG...AGGCA A U GAUGU...UCG-3'@LB@ CCACGGAAUGG AC UCAG GA UGGUGGC@LB@ \|\|\|\|::\|\|\|\|\| \|\| \|\|\|: \|\| ::\|\|\|\|\|@LB@ GGUGUUUUACC UG AGUU CU GUCACCG@LB@3'-UUU...CCCGA CC U U UUUC -5'@LB@ \| \|@LB@ 35 1@LB@@LB@@LB@Energy -12.76 kcal/mol@LB@Hybridization Energy -27.9886 kcal/mol@LB@Unfolding Energy - mRNA 10.9582 kcal/mol@LB@Unfolding Energy - sRNA 4.26884 kcal/mol@LB@@LB@Position - mRNA 84 -- 111@LB@Position - sRNA 1 -- 34@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 1 -- 7@LB@
5	2.356e-05	0.01653	b1123	potD	-19.53	0.001282	190 -- 207	9 -- 25	spermidine/putrescine ABC transporter periplasmic binding protein		_____________189________________208\n_____________\|__________________\|\n5'-GAC...ACACA____________U_____AAUGG...ACU-3'\n______________GGGGACGUUAAA_GAAAA\n______________\|\|\|\|\|\|:\|:\|\|\|_\|\|\|\|\|\n______________CCCCUGUAGUUU_CUUUU\n3'-UUU...UUUUA__________________CGUCACCG-5'\n_____________\|__________________\|\n_____________26_________________8\n	195 -- 201	14 -- 20	-22.5319 kcal/mol	2.32696 kcal/mol	0.671024 kcal/mol	189 208@LB@ \| \|@LB@5'-GAC...ACACA U AAUGG...ACU-3'@LB@ GGGGACGUUAAA GAAAA@LB@ \|\|\|\|\|\|:\|:\|\|\| \|\|\|\|\|@LB@ CCCCUGUAGUUU CUUUU@LB@3'-UUU...UUUUA CGUCACCG-5'@LB@ \| \|@LB@ 26 8@LB@@LB@@LB@Energy -19.53 kcal/mol@LB@Hybridization Energy -22.5319 kcal/mol@LB@Unfolding Energy - mRNA 2.32696 kcal/mol@LB@Unfolding Energy - sRNA 0.671024 kcal/mol@LB@@LB@Position - mRNA 190 -- 207@LB@Position - sRNA 9 -- 25@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 14 -- 20@LB@
14	0.0006392	0.1243	b3745	viaA	-13.90	0.050132	246 -- 264	10 -- 25	stimulator of RavA ATPase activity; von Willebrand factor domain protein		_____________245_________________265\n_____________\|___________________\|\n5'-ACG...GCGAA_A_____UG__________UGAUC...CGG-3'\n______________G_GGGAU__AUCGAAGAGA\n______________\|_\|\|\|\|:__\|\|\|:\|\|\|\|:\|\n______________C_CCCUG__UAGUUUCUUU\n3'-UUU...UUUUA___________________UCGUC...CCG-5'\n_____________\|___________________\|\n_____________26__________________9\n	258 -- 264	10 -- 16	-16.9183 kcal/mol	2.35086 kcal/mol	0.670388 kcal/mol	245 265@LB@ \| \|@LB@5'-ACG...GCGAA A UG UGAUC...CGG-3'@LB@ G GGGAU AUCGAAGAGA@LB@ \| \|\|\|\|: \|\|\|:\|\|\|\|:\|@LB@ C CCCUG UAGUUUCUUU@LB@3'-UUU...UUUUA UCGUC...CCG-5'@LB@ \| \|@LB@ 26 9@LB@@LB@@LB@Energy -13.90 kcal/mol@LB@Hybridization Energy -16.9183 kcal/mol@LB@Unfolding Energy - mRNA 2.35086 kcal/mol@LB@Unfolding Energy - sRNA 0.670388 kcal/mol@LB@@LB@Position - mRNA 246 -- 264@LB@Position - sRNA 10 -- 25@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 10 -- 16@LB@
40	0.001973	0.1648	b1823	cspC	-10.89	0.196769	199 -- 231	1 -- 29	stress protein member of the CspA-family		_____________198_______________________________232\n_____________\|_________________________________\|\n5'-UAU...UUUUC______CAA_G________UC____UA______UCAAC...UCU-3'\n______________AAAUGG___A_AUUAAAGG__AGGU__AGUGGU\n______________\|\|\|\|\|\|___\|_\|\|:\|\|\|\|:__\|:\|:__\|\|\|\|\|:\n______________UUUACC___U_UAGUUUCU__UUCG__UCACCG\n3'-UUU...GGUGU______CC__G________U_____________-5'\n_____________\|________________________________\|\n_____________30_______________________________1\n	210 -- 216	13 -- 19	-20.1954 kcal/mol	6.78842 kcal/mol	2.51907 kcal/mol	198 232@LB@ \| \|@LB@5'-UAU...UUUUC CAA G UC UA UCAAC...UCU-3'@LB@ AAAUGG A AUUAAAGG AGGU AGUGGU@LB@ \|\|\|\|\|\| \| \|\|:\|\|\|\|: \|:\|: \|\|\|\|\|:@LB@ UUUACC U UAGUUUCU UUCG UCACCG@LB@3'-UUU...GGUGU CC G U -5'@LB@ \| \|@LB@ 30 1@LB@@LB@@LB@Energy -10.89 kcal/mol@LB@Hybridization Energy -20.1954 kcal/mol@LB@Unfolding Energy - mRNA 6.78842 kcal/mol@LB@Unfolding Energy - sRNA 2.51907 kcal/mol@LB@@LB@Position - mRNA 199 -- 231@LB@Position - sRNA 1 -- 29@LB@Position Seed - mRNA 210 -- 216@LB@Position Seed - sRNA 13 -- 19@LB@
27	0.001238	0.1512	b4045	yjbJ	-9.99	0.275707	259 -- 267	19 -- 28	stress-induced protein UPF0337 family		_____________258________268\n_____________\|__________\|\n5'-CGU...AGAGC__________AACUG...AAG-3'\n______________AAUGGGG_CA\n______________\|\|\|\|\|\|\|_\|\|\n______________UUACCCC_GU\n3'-UUU...GUGUU_______U__AGUUU...CCG-5'\n_____________\|__________\|\n_____________29_________18\n	259 -- 265	22 -- 28	-11.8839 kcal/mol	1.24365 kcal/mol	0.649553 kcal/mol	258 268@LB@ \| \|@LB@5'-CGU...AGAGC AACUG...AAG-3'@LB@ AAUGGGG CA@LB@ \|\|\|\|\|\|\| \|\|@LB@ UUACCCC GU@LB@3'-UUU...GUGUU U AGUUU...CCG-5'@LB@ \| \|@LB@ 29 18@LB@@LB@@LB@Energy -9.99 kcal/mol@LB@Hybridization Energy -11.8839 kcal/mol@LB@Unfolding Energy - mRNA 1.24365 kcal/mol@LB@Unfolding Energy - sRNA 0.649553 kcal/mol@LB@@LB@Position - mRNA 259 -- 267@LB@Position - sRNA 19 -- 28@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 22 -- 28@LB@
31	0.001481	0.1635	b3468	yhhN	-14.35	0.039524	32 -- 52	1 -- 21	TMEM86 family putative inner membrane protein		_____________31____________________53\n_____________\|_____________________\|\n5'-CGA...UGAUA___A__________A______UUGCU...UAA-3'\n______________ACA_UAAAGAAAAG_GGUGGU\n______________\|\|\|_:\|\|\|\|\|\|\|\|\|_:\|\|\|\|:\n______________UGU_GUUUCUUUUC_UCACCG\n3'-UUU...ACCCC___A__________G______-5'\n_____________\|____________________\|\n_____________22___________________1\n	39 -- 45	8 -- 14	-21.1877 kcal/mol	5.32481 kcal/mol	1.51103 kcal/mol	31 53@LB@ \| \|@LB@5'-CGA...UGAUA A A UUGCU...UAA-3'@LB@ ACA UAAAGAAAAG GGUGGU@LB@ \|\|\| :\|\|\|\|\|\|\|\|\| :\|\|\|\|:@LB@ UGU GUUUCUUUUC UCACCG@LB@3'-UUU...ACCCC A G -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -14.35 kcal/mol@LB@Hybridization Energy -21.1877 kcal/mol@LB@Unfolding Energy - mRNA 5.32481 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 32 -- 52@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 8 -- 14@LB@
67	0.003548	0.1858	b4412	hokC	-18.22	0.003564	16 -- 36	7 -- 32	toxic membrane protein small	b1562 b0018	_____________15_________________________37\n_____________\|__________________________\|\n5'-AUU...AUAAA__________________________GAUAU...ACA-3'\n______________ACAAAAUGGG____UUAAAGAGA_GC\n______________\|\|\|\|\|\|\|\|\|\|____\|:\|\|\|\|\|:\|_\|\|\n______________UGUUUUACCC____AGUUUCUUU_CG\n3'-UUU...CGAGG__________CUGU_________U__UCACCG-5'\n_____________\|__________________________\|\n_____________33_________________________6\n	28 -- 34	10 -- 16	-22.2189 kcal/mol	1.46836 kcal/mol	2.53437 kcal/mol	15 37@LB@ \| \|@LB@5'-AUU...AUAAA GAUAU...ACA-3'@LB@ ACAAAAUGGG UUAAAGAGA GC@LB@ \|\|\|\|\|\|\|\|\|\| \|:\|\|\|\|\|:\| \|\|@LB@ UGUUUUACCC AGUUUCUUU CG@LB@3'-UUU...CGAGG CUGU U UCACCG-5'@LB@ \| \|@LB@ 33 6@LB@@LB@@LB@Energy -18.22 kcal/mol@LB@Hybridization Energy -22.2189 kcal/mol@LB@Unfolding Energy - mRNA 1.46836 kcal/mol@LB@Unfolding Energy - sRNA 2.53437 kcal/mol@LB@@LB@Position - mRNA 16 -- 36@LB@Position - sRNA 7 -- 32@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 10 -- 16@LB@
116	0.007938	0.2401	b0008	talB	-12.38	0.104625	254 -- 276	7 -- 26	transaldolase B		_____________253_____________________277\n_____________\|_______________________\|\n5'-AGG...GACAC___________C__CAAU_____UGUAU...CAA-3'\n______________UGGGGACAUCG_GG____GAAGC\n______________\|\|\|\|\|\|\|\|\|\|:_:\|____:\|\|\|\|\n______________ACCCCUGUAGU_UC____UUUCG\n3'-UUU...GUUUU___________U__U________UCACCG-5'\n_____________\|_______________________\|\n_____________27______________________6\n	258 -- 264	16 -- 22	-23.5048 kcal/mol	10.4229 kcal/mol	0.706388 kcal/mol	253 277@LB@ \| \|@LB@5'-AGG...GACAC C CAAU UGUAU...CAA-3'@LB@ UGGGGACAUCG GG GAAGC@LB@ \|\|\|\|\|\|\|\|\|\|: :\| :\|\|\|\|@LB@ ACCCCUGUAGU UC UUUCG@LB@3'-UUU...GUUUU U U UCACCG-5'@LB@ \| \|@LB@ 27 6@LB@@LB@@LB@Energy -12.38 kcal/mol@LB@Hybridization Energy -23.5048 kcal/mol@LB@Unfolding Energy - mRNA 10.4229 kcal/mol@LB@Unfolding Energy - sRNA 0.706388 kcal/mol@LB@@LB@Position - mRNA 254 -- 276@LB@Position - sRNA 7 -- 26@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 16 -- 22@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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