Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
38	0.001836	0.1648	b3188	sfsB	-13.60	0.058336	253 -- 274	1 -- 32	malPQ operon transcriptional activator	_____________252______________________________275\n_____________\|________________________________\|\n5'-CGG...UUUGC______A___A_____________________GGCGG...UGA-3'\n______________GCAAGA_GGG_ACGUCAA__________UGGC\n______________:\|\|\|:\|_\|\|\|_\|\|:\|\|\|\|__________\|\|\|\|\n______________UGUUUU_CCC_UGUAGUU__________ACCG\n3'-UUU...CGAGG______A___C_______UCUUUUCGUC____-5'\n_____________\|_______________________________\|\n_____________33______________________________1\n	264 -- 270	15 -- 21	-21.5209 kcal/mol	5.38314 kcal/mol	2.53929 kcal/mol	252 275@LB@ \| \|@LB@5'-CGG...UUUGC A A GGCGG...UGA-3'@LB@ GCAAGA GGG ACGUCAA UGGC@LB@ :\|\|\|:\| \|\|\| \|\|:\|\|\|\| \|\|\|\|@LB@ UGUUUU CCC UGUAGUU ACCG@LB@3'-UUU...CGAGG A C UCUUUUCGUC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -13.60 kcal/mol@LB@Hybridization Energy -21.5209 kcal/mol@LB@Unfolding Energy - mRNA 5.38314 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 253 -- 274@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 15 -- 21@LB@
105	0.007004	0.2333	b4034	malE	-12.62	0.093531	92 -- 123	2 -- 32	maltose transporter subunit	_____________91_________________________________124\n_____________\|__________________________________\|\n5'-AGG...ACAAA____C_________________C___G__GA___AAAGA...UGG-3'\n______________GCGA_GGUGGGG_CGU_AGGGG_AAG_AG__UGG\n______________:\|:\|_::\|\|\|\|\|_\|:\|_\|::\|:_\|\|\|_\|\|__\|\|\|\n______________UGUU_UUACCCC_GUA_UUUCU_UUC_UC__ACC\n3'-UUU...CGAGG____________U___G_____U___G_______G-5'\n_____________\|__________________________________\|\n_____________33_________________________________1\n	97 -- 103	22 -- 28	-24.1065 kcal/mol	8.94734 kcal/mol	2.53929 kcal/mol	91 124@LB@ \| \|@LB@5'-AGG...ACAAA C C G GA AAAGA...UGG-3'@LB@ GCGA GGUGGGG CGU AGGGG AAG AG UGG@LB@ :\|:\| ::\|\|\|\|\| \|:\| \|::\|: \|\|\| \|\| \|\|\|@LB@ UGUU UUACCCC GUA UUUCU UUC UC ACC@LB@3'-UUU...CGAGG U G U G G-5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -12.62 kcal/mol@LB@Hybridization Energy -24.1065 kcal/mol@LB@Unfolding Energy - mRNA 8.94734 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 92 -- 123@LB@Position - sRNA 2 -- 32@LB@Position Seed - mRNA 97 -- 103@LB@Position Seed - sRNA 22 -- 28@LB@
87	0.005508	0.2147	b0083	ftsL	-13.20	0.070894	129 -- 146	1 -- 23	membrane bound cell division leucine zipper septum protein	_____________128_____________________147\n_____________\|_______________________\|\n5'-CGU...AUGCC_______________________UGAGA...UGC-3'\n______________GGGC___GAAGAAGAG__GUGGC\n______________\|\|:\|___:\|\|\|\|\|:\|\|__\|\|\|\|\|\n______________CCUG___UUUCUUUUC__CACCG\n3'-UUU...UUACC____UAG_________GU_____-5'\n_____________\|______________________\|\n_____________24_____________________1\n	135 -- 141	8 -- 14	-20.4792 kcal/mol	5.69631 kcal/mol	1.57928 kcal/mol	128 147@LB@ \| \|@LB@5'-CGU...AUGCC UGAGA...UGC-3'@LB@ GGGC GAAGAAGAG GUGGC@LB@ \|\|:\| :\|\|\|\|\|:\|\| \|\|\|\|\|@LB@ CCUG UUUCUUUUC CACCG@LB@3'-UUU...UUACC UAG GU -5'@LB@ \| \|@LB@ 24 1@LB@@LB@@LB@Energy -13.20 kcal/mol@LB@Hybridization Energy -20.4792 kcal/mol@LB@Unfolding Energy - mRNA 5.69631 kcal/mol@LB@Unfolding Energy - sRNA 1.57928 kcal/mol@LB@@LB@Position - mRNA 129 -- 146@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 8 -- 14@LB@
53	0.002642	0.1735	b0055	djlA	-12.56	0.096184	186 -- 227	1 -- 31	membrane-anchored DnaK co-chaperone DNA-binding protein	_____________185________________________________________228\n_____________\|__________________________________________\|\n5'-GAC...UGUGU___UU______AUAUAUGCAG____________AU__U____GUGGC...UUG-3'\n______________CGA__UGGGGA__________UAUUGGGGAAAA__CA_UGGC\n______________\|:\|__\|\|\|\|\|\|__________:\|\|::::\|\|\|\|\|__\|\|_\|\|\|\|\n______________GUU__ACCCCU__________GUAGUUUCUUUU__GU_ACCG\n3'-UUU...GAGGU___UU____________________________C___C____-5'\n_____________\|_________________________________________\|\n_____________32________________________________________1\n	212 -- 218	9 -- 15	-25.7115 kcal/mol	10.6128 kcal/mol	2.53929 kcal/mol	185 228@LB@ \| \|@LB@5'-GAC...UGUGU UU AUAUAUGCAG AU U GUGGC...UUG-3'@LB@ CGA UGGGGA UAUUGGGGAAAA CA UGGC@LB@ \|:\| \|\|\|\|\|\| :\|\|::::\|\|\|\|\| \|\| \|\|\|\|@LB@ GUU ACCCCU GUAGUUUCUUUU GU ACCG@LB@3'-UUU...GAGGU UU C C -5'@LB@ \| \|@LB@ 32 1@LB@@LB@@LB@Energy -12.56 kcal/mol@LB@Hybridization Energy -25.7115 kcal/mol@LB@Unfolding Energy - mRNA 10.6128 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 186 -- 227@LB@Position - sRNA 1 -- 31@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 9 -- 15@LB@
2	5.107e-11	8.961e-08	b2314	dedD	-25.37	0.000001	176 -- 205	1 -- 30	membrane-anchored periplasmic protein involved in septation	_____________175_____________________________206\n_____________\|_______________________________\|\n5'-UUG...UCAUG______AUU______________________AAGUA...AUU-3'\n______________GAAGUG___GACG_CGAGGAGAAGCGGUGGC\n______________:\|\|:\|\|___\|\|\|:_\|:\|:\|\|:\|\|\|\|:\|\|\|\|\|\n______________UUUUAC___CUGU_GUUUCUUUUCGUCACCG\n3'-UUU...AGGUG______CC_____A_________________-5'\n_____________\|______________________________\|\n_____________31_____________________________1\n	199 -- 205	1 -- 7	-36.5756 kcal/mol	8.68219 kcal/mol	2.51911 kcal/mol	175 206@LB@ \| \|@LB@5'-UUG...UCAUG AUU AAGUA...AUU-3'@LB@ GAAGUG GACG CGAGGAGAAGCGGUGGC@LB@ :\|\|:\|\| \|\|\|: \|:\|:\|\|:\|\|\|\|:\|\|\|\|\|@LB@ UUUUAC CUGU GUUUCUUUUCGUCACCG@LB@3'-UUU...AGGUG CC A -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -25.37 kcal/mol@LB@Hybridization Energy -36.5756 kcal/mol@LB@Unfolding Energy - mRNA 8.68219 kcal/mol@LB@Unfolding Energy - sRNA 2.51911 kcal/mol@LB@@LB@Position - mRNA 176 -- 205@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 199 -- 205@LB@Position Seed - sRNA 1 -- 7@LB@
36	0.001784	0.1648	b0785	moaE	-14.28	0.040953	241 -- 264	1 -- 21	molybdopterin synthase large subunit	_____________240______________________265\n_____________\|________________________\|\n5'-AGA...GUUCA____A___________CCC_____UGGCG...CCU-3'\n______________GCGU_GGAGAAGAGUA___GUGGC\n______________:\|:\|_::\|\|\|\|:\|\|:\|___\|\|\|\|\|\n______________UGUA_UUUCUUUUCGU___CACCG\n3'-UUU...ACCCC____G___________________-5'\n_____________\|_______________________\|\n_____________22______________________1\n	250 -- 256	6 -- 12	-21.7367 kcal/mol	5.94077 kcal/mol	1.51103 kcal/mol	240 265@LB@ \| \|@LB@5'-AGA...GUUCA A CCC UGGCG...CCU-3'@LB@ GCGU GGAGAAGAGUA GUGGC@LB@ :\|:\| ::\|\|\|\|:\|\|:\| \|\|\|\|\|@LB@ UGUA UUUCUUUUCGU CACCG@LB@3'-UUU...ACCCC G -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -14.28 kcal/mol@LB@Hybridization Energy -21.7367 kcal/mol@LB@Unfolding Energy - mRNA 5.94077 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 241 -- 264@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 6 -- 12@LB@
110	0.007401	0.2348	b0784	moaD	-14.78	0.031320	178 -- 203	3 -- 25	molybdopterin synthase small subunit	_____________177________________________204\n_____________\|__________________________\|\n5'-GCA...AAGUC___U___U______U________A__AUUAA...AGC-3'\n______________GGG_GAC_UUAAGG_GGAAGCGG_UG\n______________\|\|\|_\|\|\|_\|:\|\|:\|_::\|\|\|\|:\|_\|\|\n______________CCC_CUG_AGUUUC_UUUUCGUC_AC\n3'-UUU...UUUUA_______U__________________CG-5'\n_____________\|__________________________\|\n_____________26_________________________2\n	194 -- 200	5 -- 11	-23.9822 kcal/mol	6.68375 kcal/mol	2.5166 kcal/mol	177 204@LB@ \| \|@LB@5'-GCA...AAGUC U U U A AUUAA...AGC-3'@LB@ GGG GAC UUAAGG GGAAGCGG UG@LB@ \|\|\| \|\|\| \|:\|\|:\| ::\|\|\|\|:\| \|\|@LB@ CCC CUG AGUUUC UUUUCGUC AC@LB@3'-UUU...UUUUA U CG-5'@LB@ \| \|@LB@ 26 2@LB@@LB@@LB@Energy -14.78 kcal/mol@LB@Hybridization Energy -23.9822 kcal/mol@LB@Unfolding Energy - mRNA 6.68375 kcal/mol@LB@Unfolding Energy - sRNA 2.5166 kcal/mol@LB@@LB@Position - mRNA 178 -- 203@LB@Position - sRNA 3 -- 25@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 5 -- 11@LB@
73	0.004089	0.1966	b3857	mobA	-16.43	0.011914	256 -- 282	7 -- 29	molybdopterin-guanine dinucleotide synthase	_____________255_________________________283\n_____________\|___________________________\|\n5'-UCC...CAGAC_______C_____AGA_______UU__UUGAA...AAC-3'\n______________GAAUGGG_GGCGU___UAAAGGA__GC\n______________:\|\|\|\|\|\|_\|:\|:\|___:\|\|\|\|:\|__\|\|\n______________UUUACCC_CUGUA___GUUUCUU__CG\n3'-UUU...GGUGU_______________________UU__UCACCG-5'\n_____________\|___________________________\|\n_____________30__________________________6\n	272 -- 278	11 -- 17	-21.4093 kcal/mol	4.27411 kcal/mol	0.706428 kcal/mol	255 283@LB@ \| \|@LB@5'-UCC...CAGAC C AGA UU UUGAA...AAC-3'@LB@ GAAUGGG GGCGU UAAAGGA GC@LB@ :\|\|\|\|\|\| \|:\|:\| :\|\|\|\|:\| \|\|@LB@ UUUACCC CUGUA GUUUCUU CG@LB@3'-UUU...GGUGU UU UCACCG-5'@LB@ \| \|@LB@ 30 6@LB@@LB@@LB@Energy -16.43 kcal/mol@LB@Hybridization Energy -21.4093 kcal/mol@LB@Unfolding Energy - mRNA 4.27411 kcal/mol@LB@Unfolding Energy - sRNA 0.706428 kcal/mol@LB@@LB@Position - mRNA 256 -- 282@LB@Position - sRNA 7 -- 29@LB@Position Seed - mRNA 272 -- 278@LB@Position Seed - sRNA 11 -- 17@LB@
119	0.008338	0.2459	b0123	cueO	-14.31	0.040489	47 -- 61	9 -- 24	multicopper oxidase (laccase)	_____________46_______________62\n_____________\|________________\|\n5'-CUG...UACCA________________UAAAC...CGU-3'\n______________GGGGC_UUAAAGAAGA\n______________\|\|\|:\|_\|:\|\|\|\|\|\|:\|\n______________CCCUG_AGUUUCUUUU\n3'-UUU...UUUAC_____U__________CGUCACCG-5'\n_____________\|________________\|\n_____________25_______________8\n	55 -- 61	9 -- 15	-17.0719 kcal/mol	2.10565 kcal/mol	0.659805 kcal/mol	46 62@LB@ \| \|@LB@5'-CUG...UACCA UAAAC...CGU-3'@LB@ GGGGC UUAAAGAAGA@LB@ \|\|\|:\| \|:\|\|\|\|\|\|:\|@LB@ CCCUG AGUUUCUUUU@LB@3'-UUU...UUUAC U CGUCACCG-5'@LB@ \| \|@LB@ 25 8@LB@@LB@@LB@Energy -14.31 kcal/mol@LB@Hybridization Energy -17.0719 kcal/mol@LB@Unfolding Energy - mRNA 2.10565 kcal/mol@LB@Unfolding Energy - sRNA 0.659805 kcal/mol@LB@@LB@Position - mRNA 47 -- 61@LB@Position - sRNA 9 -- 24@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 9 -- 15@LB@
72	0.004007	0.1953	b2175	mepS	-13.55	0.059767	177 -- 194	6 -- 23	murein DD-endopeptidase space-maker hydrolase mutational suppressor of prc thermosensitivity outer membrane lipoprotein weak murein LD-carboxypeptidase	_____________176________________195\n_____________\|__________________\|\n5'-CAA...GUUAA____U__________A__AACAA...CAA-3'\n______________GGAC_UCAAGGGAAA_CA\n______________\|\|\|\|_\|\|\|\|:\|:\|\|\|_\|\|\n______________CCUG_AGUUUCUUUU_GU\n3'-UUU...UUACC____U__________C__CACCG-5'\n_____________\|__________________\|\n_____________24_________________5\n	185 -- 191	9 -- 15	-17.1277 kcal/mol	2.97845 kcal/mol	0.599171 kcal/mol	176 195@LB@ \| \|@LB@5'-CAA...GUUAA U A AACAA...CAA-3'@LB@ GGAC UCAAGGGAAA CA@LB@ \|\|\|\| \|\|\|\|:\|:\|\|\| \|\|@LB@ CCUG AGUUUCUUUU GU@LB@3'-UUU...UUACC U C CACCG-5'@LB@ \| \|@LB@ 24 5@LB@@LB@@LB@Energy -13.55 kcal/mol@LB@Hybridization Energy -17.1277 kcal/mol@LB@Unfolding Energy - mRNA 2.97845 kcal/mol@LB@Unfolding Energy - sRNA 0.599171 kcal/mol@LB@@LB@Position - mRNA 177 -- 194@LB@Position - sRNA 6 -- 23@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 9 -- 15@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching