Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
88	0.005527	0.2147	b3525	yhjH	-13.65	0.056862	190 -- 212	5 -- 23	cyclic-di-GMP phosphodiesterase FlhDC-regulated		_____________189_____________________213\n_____________\|_______________________\|\n5'-UUA...ACACA_______UUU__UGAU_______GUUAU...CCU-3'\n______________GGACAUC___GA____AAGGCAG\n______________\|\|\|\|\|\|\|___\|\|____\|\|:\|\|\|\|\n______________CCUGUAG___CU____UUUCGUC\n3'-UUU...UUACC_______UUU_____________ACCG-5'\n_____________\|_______________________\|\n_____________24______________________4\n	206 -- 212	5 -- 11	-19.0549 kcal/mol	3.83037 kcal/mol	1.57523 kcal/mol	189 213@LB@ \| \|@LB@5'-UUA...ACACA UUU UGAU GUUAU...CCU-3'@LB@ GGACAUC GA AAGGCAG@LB@ \|\|\|\|\|\|\| \|\| \|\|:\|\|\|\|@LB@ CCUGUAG CU UUUCGUC@LB@3'-UUU...UUACC UUU ACCG-5'@LB@ \| \|@LB@ 24 4@LB@@LB@@LB@Energy -13.65 kcal/mol@LB@Hybridization Energy -19.0549 kcal/mol@LB@Unfolding Energy - mRNA 3.83037 kcal/mol@LB@Unfolding Energy - sRNA 1.57523 kcal/mol@LB@@LB@Position - mRNA 190 -- 212@LB@Position - sRNA 5 -- 23@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 5 -- 11@LB@
131	0.009354	0.2491	b4478	dgoD	-14.24	0.041949	180 -- 199	1 -- 22	D-galactonate dehydratase		_____________179____________________200\n_____________\|______________________\|\n5'-AAA...GUUAA_____AUC______________AAUGA...UGA-3'\n______________GGCGU____GAGAGGCAGUG_C\n______________\|:\|:\|____\|\|:\|:\|\|\|\|\|\|_\|\n______________CUGUA____CUUUUCGUCAC_G\n3'-UUU...UACCC_____GUUU___________C_-5'\n_____________\|_____________________\|\n_____________23____________________1\n	192 -- 198	3 -- 9	-21.1696 kcal/mol	5.38995 kcal/mol	1.54023 kcal/mol	179 200@LB@ \| \|@LB@5'-AAA...GUUAA AUC AAUGA...UGA-3'@LB@ GGCGU GAGAGGCAGUG C@LB@ \|:\|:\| \|\|:\|:\|\|\|\|\|\| \|@LB@ CUGUA CUUUUCGUCAC G@LB@3'-UUU...UACCC GUUU C -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -14.24 kcal/mol@LB@Hybridization Energy -21.1696 kcal/mol@LB@Unfolding Energy - mRNA 5.38995 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 180 -- 199@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 3 -- 9@LB@
43	0.00215	0.1648	b4268	idnK	-13.73	0.054596	180 -- 268	1 -- 79	D-gluconate kinase thermosensitive	b3437	_____________179______________________________________________________________________________________________269\n_____________\|________________________________________________________________________________________________\|\n5'-AGG...AGGUA___U______GGA______________________U_AAAGCUUUAUU_________GU____G_______UAAA_______UUGGU____A____UGCCG...AUG-3'\n______________AGA_GAUUCA___GAUUUU______AAAUGGCGGG_G___________UUGAUGGGC__UUCA_GGAGUGG____ACAUUAA_____AGCA__GGU\n______________\|:\|_:\|::\|\|___:\|::\|\|______\|\|\|\|\|\|\|\|\|\|_\|___________\|\|\|\|\|\|\|\|\|__\|:\|\|_::\|:\|\|\|____\|\|\|\|:\|\|_____\|\|\|\|__\|\|:\n______________UUU_UUGGGU___UUGGAA______UUUACCGCCC_C___________AACUACCCG__AGGU_UUUUACC____UGUAGUU_____UCGU__CCG\n___________3'-___U______AG_______CUUGGC______________________________________G_______CC_________UCUUU____CA___-5'\n______________\|______________________________________________________________________________________________\|\n______________79_____________________________________________________________________________________________1\n	249 -- 255	15 -- 21	-57.0668 kcal/mol	16.9855 kcal/mol	26.3515 kcal/mol	179 269@LB@ \| \|@LB@5'-AGG...AGGUA U GGA U AAAGCUUUAUU GU G UAAA UUGGU A UGCCG...AUG-3'@LB@ AGA GAUUCA GAUUUU AAAUGGCGGG G UUGAUGGGC UUCA GGAGUGG ACAUUAA AGCA GGU@LB@ \|:\| :\|::\|\| :\|::\|\| \|\|\|\|\|\|\|\|\|\| \| \|\|\|\|\|\|\|\|\| \|:\|\| ::\|:\|\|\| \|\|\|\|:\|\| \|\|\|\| \|\|:@LB@ UUU UUGGGU UUGGAA UUUACCGCCC C AACUACCCG AGGU UUUUACC UGUAGUU UCGU CCG@LB@ 3'- U AG CUUGGC G CC UCUUU CA -5'@LB@ \| \|@LB@ 79 1@LB@@LB@@LB@Energy -13.73 kcal/mol@LB@Hybridization Energy -57.0668 kcal/mol@LB@Unfolding Energy - mRNA 16.9855 kcal/mol@LB@Unfolding Energy - sRNA 26.3515 kcal/mol@LB@@LB@Position - mRNA 180 -- 268@LB@Position - sRNA 1 -- 79@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 15 -- 21@LB@
169	0.01228	0.2504	b3386	rpe	-13.36	0.065831	171 -- 203	3 -- 33	D-ribulose-5-phosphate 3-epimerase		_____________170_______________________________204\n_____________\|_________________________________\|\n5'-CGC...UCACC_______AAAUAAUUC______________A__AAACA...UGC-3'\n______________CGCGAAA_________UCAAGGAGAAGCGG_UG\n______________\|:\|:\|\|\|_________\|\|\|\|:\|\|:\|\|\|\|:\|_\|\|\n______________GUGUUUU_________AGUUUCUUUUCGUC_AC\n3'-UUU...CCGAG_______ACCCCUGU__________________CG-5'\n_____________\|_________________________________\|\n_____________34________________________________2\n	194 -- 200	5 -- 11	-23.2607 kcal/mol	6.82691 kcal/mol	3.0786 kcal/mol	170 204@LB@ \| \|@LB@5'-CGC...UCACC AAAUAAUUC A AAACA...UGC-3'@LB@ CGCGAAA UCAAGGAGAAGCGG UG@LB@ \|:\|:\|\|\| \|\|\|\|:\|\|:\|\|\|\|:\| \|\|@LB@ GUGUUUU AGUUUCUUUUCGUC AC@LB@3'-UUU...CCGAG ACCCCUGU CG-5'@LB@ \| \|@LB@ 34 2@LB@@LB@@LB@Energy -13.36 kcal/mol@LB@Hybridization Energy -23.2607 kcal/mol@LB@Unfolding Energy - mRNA 6.82691 kcal/mol@LB@Unfolding Energy - sRNA 3.0786 kcal/mol@LB@@LB@Position - mRNA 171 -- 203@LB@Position - sRNA 3 -- 33@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 5 -- 11@LB@
132	0.00937	0.2491	b1120	cobB	-12.17	0.114836	175 -- 191	3 -- 18	deacetylase of acs and cheY chemotaxis regulator		_____________174_______________192\n_____________\|_________________\|\n5'-CGC...UUUUU_______U_________CCGGA...AUC-3'\n______________UCAGAGA_AAAGUGGUG\n______________\|\|\|:\|\|\|_\|\|\|\|::\|\|\|\n______________AGUUUCU_UUUCGUCAC\n3'-UUU...CCUGU_________________CG-5'\n_____________\|_________________\|\n_____________19________________2\n	185 -- 191	3 -- 9	-17.0979 kcal/mol	3.47557 kcal/mol	1.45388 kcal/mol	174 192@LB@ \| \|@LB@5'-CGC...UUUUU U CCGGA...AUC-3'@LB@ UCAGAGA AAAGUGGUG@LB@ \|\|\|:\|\|\| \|\|\|\|::\|\|\|@LB@ AGUUUCU UUUCGUCAC@LB@3'-UUU...CCUGU CG-5'@LB@ \| \|@LB@ 19 2@LB@@LB@@LB@Energy -12.17 kcal/mol@LB@Hybridization Energy -17.0979 kcal/mol@LB@Unfolding Energy - mRNA 3.47557 kcal/mol@LB@Unfolding Energy - sRNA 1.45388 kcal/mol@LB@@LB@Position - mRNA 175 -- 191@LB@Position - sRNA 3 -- 18@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 3 -- 9@LB@
111	0.007434	0.2348	b0103	coaE	-19.66	0.001155	224 -- 242	1 -- 25	dephospho-CoA kinase		_____________223________________________243\n_____________\|__________________________\|\n5'-UAU...UUAAC___A______________________AAGAG...AUA-3'\n______________GGG_GGCAUU_______GGCAGUGGC\n______________\|\|\|_\|:\|\|\|:_______:\|\|\|\|\|\|\|\|\n______________CCC_CUGUAG_______UCGUCACCG\n3'-UUU...UUUUA__________UUUCUUU_________-5'\n_____________\|_________________________\|\n_____________26________________________1\n	236 -- 242	1 -- 7	-28.3462 kcal/mol	6.16994 kcal/mol	2.51748 kcal/mol	223 243@LB@ \| \|@LB@5'-UAU...UUAAC A AAGAG...AUA-3'@LB@ GGG GGCAUU GGCAGUGGC@LB@ \|\|\| \|:\|\|\|: :\|\|\|\|\|\|\|\|@LB@ CCC CUGUAG UCGUCACCG@LB@3'-UUU...UUUUA UUUCUUU -5'@LB@ \| \|@LB@ 26 1@LB@@LB@@LB@Energy -19.66 kcal/mol@LB@Hybridization Energy -28.3462 kcal/mol@LB@Unfolding Energy - mRNA 6.16994 kcal/mol@LB@Unfolding Energy - sRNA 2.51748 kcal/mol@LB@@LB@Position - mRNA 224 -- 242@LB@Position - sRNA 1 -- 25@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 1 -- 7@LB@
190	0.01401	0.2529	b2478	dapA	-10.08	0.266807	73 -- 98	6 -- 25	dihydrodipicolinate synthase		_____________72_________________________99\n_____________\|__________________________\|\n5'-GGC...UUUAA____UGA____UUCUU__________UAAAA...CCA-3'\n______________GGGG___GUUG_____AAGGAAAGCA\n______________\|\|\|\|___:\|::_____\|\|\|:\|\|\|\|\|\|\n______________CCCC___UAGU_____UUCUUUUCGU\n3'-UUU...UUUUA____UG____________________CACCG-5'\n_____________\|__________________________\|\n_____________26_________________________5\n	92 -- 98	6 -- 12	-17.2074 kcal/mol	6.41889 kcal/mol	0.70636 kcal/mol	72 99@LB@ \| \|@LB@5'-GGC...UUUAA UGA UUCUU UAAAA...CCA-3'@LB@ GGGG GUUG AAGGAAAGCA@LB@ \|\|\|\| :\|:: \|\|\|:\|\|\|\|\|\|@LB@ CCCC UAGU UUCUUUUCGU@LB@3'-UUU...UUUUA UG CACCG-5'@LB@ \| \|@LB@ 26 5@LB@@LB@@LB@Energy -10.08 kcal/mol@LB@Hybridization Energy -17.2074 kcal/mol@LB@Unfolding Energy - mRNA 6.41889 kcal/mol@LB@Unfolding Energy - sRNA 0.70636 kcal/mol@LB@@LB@Position - mRNA 73 -- 98@LB@Position - sRNA 6 -- 25@LB@Position Seed - mRNA 92 -- 98@LB@Position Seed - sRNA 6 -- 12@LB@
161	0.01187	0.2504	b0093	ftsQ	-14.15	0.043981	32 -- 43	18 -- 29	divisome assembly protein membrane anchored protein involved in growth of wall at septum		_____________31___________44\n_____________\|____________\|\n5'-GCU...GCAAA____________AUUGA...CCG-3'\n______________GGAUGGGGACGU\n______________::\|\|\|\|\|\|\|\|:\|\n______________UUUACCCCUGUA\n3'-UUU...GGUGU____________GUUUC...CCG-5'\n_____________\|____________\|\n_____________30___________17\n	37 -- 43	18 -- 24	-18.9266 kcal/mol	4.11166 kcal/mol	0.665542 kcal/mol	31 44@LB@ \| \|@LB@5'-GCU...GCAAA AUUGA...CCG-3'@LB@ GGAUGGGGACGU@LB@ ::\|\|\|\|\|\|\|\|:\|@LB@ UUUACCCCUGUA@LB@3'-UUU...GGUGU GUUUC...CCG-5'@LB@ \| \|@LB@ 30 17@LB@@LB@@LB@Energy -14.15 kcal/mol@LB@Hybridization Energy -18.9266 kcal/mol@LB@Unfolding Energy - mRNA 4.11166 kcal/mol@LB@Unfolding Energy - sRNA 0.665542 kcal/mol@LB@@LB@Position - mRNA 32 -- 43@LB@Position - sRNA 18 -- 29@LB@Position Seed - mRNA 37 -- 43@LB@Position Seed - sRNA 18 -- 24@LB@
156	0.01139	0.2504	b0197	metQ	-9.46	0.330846	169 -- 205	1 -- 33	DL-methionine transporter subunit		_____________168_____________________________________206\n_____________\|_______________________________________\|\n5'-GUG...UUCAA_____CAUAAAUA___________GAAUAA_________GUUCA...UUA-3'\n______________CACAA_________AUUGAAGAAG______GGUA_UGGC\n______________\|\|\|\|\|_________\|\|::\|\|\|\|\|:______:\|:\|_\|\|\|\|\n______________GUGUU_________UAGUUUCUUU______UCGU_ACCG\n3'-UUU...CCGAG_____UUACCCCUG____________________C____-5'\n_____________\|______________________________________\|\n_____________34_____________________________________1\n	185 -- 191	10 -- 16	-16.9822 kcal/mol	4.44131 kcal/mol	3.07861 kcal/mol	168 206@LB@ \| \|@LB@5'-GUG...UUCAA CAUAAAUA GAAUAA GUUCA...UUA-3'@LB@ CACAA AUUGAAGAAG GGUA UGGC@LB@ \|\|\|\|\| \|\|::\|\|\|\|\|: :\|:\| \|\|\|\|@LB@ GUGUU UAGUUUCUUU UCGU ACCG@LB@3'-UUU...CCGAG UUACCCCUG C -5'@LB@ \| \|@LB@ 34 1@LB@@LB@@LB@Energy -9.46 kcal/mol@LB@Hybridization Energy -16.9822 kcal/mol@LB@Unfolding Energy - mRNA 4.44131 kcal/mol@LB@Unfolding Energy - sRNA 3.07861 kcal/mol@LB@@LB@Position - mRNA 169 -- 205@LB@Position - sRNA 1 -- 33@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 10 -- 16@LB@
58	0.00302	0.1789	b0556	rzpD	-16.79	0.009474	64 -- 103	3 -- 36	DLP12 prophage; putative murein endopeptidase		_____________63_________________________________________104\n_____________\|__________________________________________\|\n5'-CGC...CGCUU___________AAACCA___C___________C_____U___AUCAG...ACA-3'\n______________CUUCGCAAAAU______GGG_GAUAUCAAAGG___GCA_GUG\n______________\|\|:\|:\|\|\|\|\|\|______\|\|\|_\|\|:\|\|\|\|\|\|\|:___\|\|\|_\|\|\|\n______________GAGGUGUUUUA______CCC_CUGUAGUUUCU___CGU_CAC\n3'-UUU...UACCC________________________________UUU_______CG-5'\n_____________\|__________________________________________\|\n_____________37_________________________________________2\n	89 -- 95	12 -- 18	-34.1904 kcal/mol	10.724 kcal/mol	6.67685 kcal/mol	63 104@LB@ \| \|@LB@5'-CGC...CGCUU AAACCA C C U AUCAG...ACA-3'@LB@ CUUCGCAAAAU GGG GAUAUCAAAGG GCA GUG@LB@ \|\|:\|:\|\|\|\|\|\| \|\|\| \|\|:\|\|\|\|\|\|\|: \|\|\| \|\|\|@LB@ GAGGUGUUUUA CCC CUGUAGUUUCU CGU CAC@LB@3'-UUU...UACCC UUU CG-5'@LB@ \| \|@LB@ 37 2@LB@@LB@@LB@Energy -16.79 kcal/mol@LB@Hybridization Energy -34.1904 kcal/mol@LB@Unfolding Energy - mRNA 10.724 kcal/mol@LB@Unfolding Energy - sRNA 6.67685 kcal/mol@LB@@LB@Position - mRNA 64 -- 103@LB@Position - sRNA 3 -- 36@LB@Position Seed - mRNA 89 -- 95@LB@Position Seed - sRNA 12 -- 18@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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