Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
8	0.0001267	0.05555	b2697	alaS	-16.66	0.010303	247 -- 274	1 -- 35	alanyl-tRNA synthetase		_____________246_________________________________275\n_____________\|___________________________________\|\n5'-CCA...CUUUU_______UAA______________________U__CAGCA...ACG-3'\n______________UCCAUAG____GGGACAUCA______GGUAGU_GC\n______________\|\|\|\|:\|:____\|\|\|\|\|\|\|\|\|______:\|:\|\|\|_\|\|\n______________AGGUGUU____CCCUGUAGU______UCGUCA_CG\n3'-UUU...ACCCG_______UUAC_________UUCUUU______C__-5'\n_____________\|__________________________________\|\n_____________36_________________________________1\n	259 -- 265	16 -- 22	-29.5579 kcal/mol	7.86268 kcal/mol	5.03645 kcal/mol	246 275@LB@ \| \|@LB@5'-CCA...CUUUU UAA U CAGCA...ACG-3'@LB@ UCCAUAG GGGACAUCA GGUAGU GC@LB@ \|\|\|\|:\|: \|\|\|\|\|\|\|\|\| :\|:\|\|\| \|\|@LB@ AGGUGUU CCCUGUAGU UCGUCA CG@LB@3'-UUU...ACCCG UUAC UUCUUU C -5'@LB@ \| \|@LB@ 36 1@LB@@LB@@LB@Energy -16.66 kcal/mol@LB@Hybridization Energy -29.5579 kcal/mol@LB@Unfolding Energy - mRNA 7.86268 kcal/mol@LB@Unfolding Energy - sRNA 5.03645 kcal/mol@LB@@LB@Position - mRNA 247 -- 274@LB@Position - sRNA 1 -- 35@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 16 -- 22@LB@
189	0.01393	0.2529	b0934	ssuC	-14.25	0.041816	137 -- 153	1 -- 17	aliphatic sulfonate ABC transporter permease		_____________136_______________154\n_____________\|_________________\|\n5'-UGC...AUCCG_____C___________GAAUG...GGC-3'\n______________CAAGG_GAAGCGGUGGC\n______________\|\|\|:\|_:\|\|\|\|:\|\|\|\|\|\n______________GUUUC_UUUCGUCACCG\n3'-UUU...CUGUA_____U___________-5'\n_____________\|________________\|\n_____________18_______________1\n	147 -- 153	1 -- 7	-24.8998 kcal/mol	9.21593 kcal/mol	1.43853 kcal/mol	136 154@LB@ \| \|@LB@5'-UGC...AUCCG C GAAUG...GGC-3'@LB@ CAAGG GAAGCGGUGGC@LB@ \|\|\|:\| :\|\|\|\|:\|\|\|\|\|@LB@ GUUUC UUUCGUCACCG@LB@3'-UUU...CUGUA U -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -14.25 kcal/mol@LB@Hybridization Energy -24.8998 kcal/mol@LB@Unfolding Energy - mRNA 9.21593 kcal/mol@LB@Unfolding Energy - sRNA 1.43853 kcal/mol@LB@@LB@Position - mRNA 137 -- 153@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 1 -- 7@LB@
64	0.003422	0.1851	b4238	nrdD	-18.31	0.003326	69 -- 106	1 -- 34	anaerobic ribonucleoside-triphosphate reductase		_____________68_____________________________________107\n_____________\|______________________________________\|\n5'-UAU...GCUCC________AA__U_C_______U___U______A____GAUUG...UCG-3'\n______________CCACGAAA__GG_G_GGCGUUG_GGA_AAGCGG_UGGC\n______________\|\|\|\|:\|\|\|__\|\|_\|_\|:\|:\|::_:\|\|_\|\|\|\|:\|_\|\|\|\|\n______________GGUGUUUU__CC_C_CUGUAGU_UCU_UUCGUC_ACCG\n3'-UUU...CCCGA________A_____________U___U___________-5'\n_____________\|_____________________________________\|\n_____________35____________________________________1\n	84 -- 90	16 -- 22	-34.2754 kcal/mol	11.6957 kcal/mol	4.26884 kcal/mol	68 107@LB@ \| \|@LB@5'-UAU...GCUCC AA U C U U A GAUUG...UCG-3'@LB@ CCACGAAA GG G GGCGUUG GGA AAGCGG UGGC@LB@ \|\|\|\|:\|\|\| \|\| \| \|:\|:\|:: :\|\| \|\|\|\|:\| \|\|\|\|@LB@ GGUGUUUU CC C CUGUAGU UCU UUCGUC ACCG@LB@3'-UUU...CCCGA A U U -5'@LB@ \| \|@LB@ 35 1@LB@@LB@@LB@Energy -18.31 kcal/mol@LB@Hybridization Energy -34.2754 kcal/mol@LB@Unfolding Energy - mRNA 11.6957 kcal/mol@LB@Unfolding Energy - sRNA 4.26884 kcal/mol@LB@@LB@Position - mRNA 69 -- 106@LB@Position - sRNA 1 -- 34@LB@Position Seed - mRNA 84 -- 90@LB@Position Seed - sRNA 16 -- 22@LB@
84	0.005241	0.2147	b2513	yfgM	-12.13	0.116945	176 -- 204	2 -- 26	ancillary SecYEG translocon subunit; putative anti-RcsB factor		_____________175___________________________205\n_____________\|_____________________________\|\n5'-GGC...GUUAC____UUAAGG_________G_CAGC____AAAUU...UUG-3'\n______________UGGG______AAGGAGAAG_A____GUGG\n______________\|\|\|\|______\|\|:\|\|:\|\|\|_\|____\|\|\|\|\n______________ACCC______UUUCUUUUC_U____CACC\n3'-UUU...GUUUU____CUGUAG_________G_________G-5'\n_____________\|_____________________________\|\n_____________27____________________________1\n	188 -- 194	8 -- 14	-19.1762 kcal/mol	4.53021 kcal/mol	2.5184 kcal/mol	175 205@LB@ \| \|@LB@5'-GGC...GUUAC UUAAGG G CAGC AAAUU...UUG-3'@LB@ UGGG AAGGAGAAG A GUGG@LB@ \|\|\|\| \|\|:\|\|:\|\|\| \| \|\|\|\|@LB@ ACCC UUUCUUUUC U CACC@LB@3'-UUU...GUUUU CUGUAG G G-5'@LB@ \| \|@LB@ 27 1@LB@@LB@@LB@Energy -12.13 kcal/mol@LB@Hybridization Energy -19.1762 kcal/mol@LB@Unfolding Energy - mRNA 4.53021 kcal/mol@LB@Unfolding Energy - sRNA 2.5184 kcal/mol@LB@@LB@Position - mRNA 176 -- 204@LB@Position - sRNA 2 -- 26@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 8 -- 14@LB@
19	0.000738	0.1243	b1508	hipB	-18.00	0.004180	266 -- 295	1 -- 32	antitoxin of HipAB toxin-antitoxin system	b1299 b1434	_____________265________________________________296\n_____________\|__________________________________\|\n5'-AUG...CGCCA_________CU___________C___________GAAAA-3'\n______________GCAAAAUGG__GGACG_CAGAG__GAGC__UGGC\n______________:\|\|\|\|\|\|\|\|__\|\|\|\|:_\|\|:\|\|__:\|\|\|__\|\|\|\|\n______________UGUUUUACC__CCUGU_GUUUC__UUCG__ACCG\n3'-UUU...CGAGG________________A_____UU____UC____-5'\n_____________\|_________________________________\|\n_____________33________________________________1\n	268 -- 274	24 -- 30	-30.0826 kcal/mol	9.54799 kcal/mol	2.53929 kcal/mol	265 296@LB@ \| \|@LB@5'-AUG...CGCCA CU C GAAAA-3'@LB@ GCAAAAUGG GGACG CAGAG GAGC UGGC@LB@ :\|\|\|\|\|\|\|\| \|\|\|\|: \|\|:\|\| :\|\|\| \|\|\|\|@LB@ UGUUUUACC CCUGU GUUUC UUCG ACCG@LB@3'-UUU...CGAGG A UU UC -5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -18.00 kcal/mol@LB@Hybridization Energy -30.0826 kcal/mol@LB@Unfolding Energy - mRNA 9.54799 kcal/mol@LB@Unfolding Energy - sRNA 2.53929 kcal/mol@LB@@LB@Position - mRNA 266 -- 295@LB@Position - sRNA 1 -- 32@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 24 -- 30@LB@
130	0.009344	0.2491	b2840	ygeA	-9.74	0.301486	266 -- 295	2 -- 29	Asp/Glu_racemase family protein		_____________265____________________________296\n_____________\|______________________________\|\n5'-AAA...UUGAU_____AA_________C__C___UU_____GCUUC-3'\n______________AAAUG__GGCAUUAAA_AG_GGC__GGUGG\n______________\|\|\|\|\|__\|:\|\|\|:\|\|\|_\|:_:\|\|__:\|\|\|\|\n______________UUUAC__CUGUAGUUU_UU_UCG__UCACC\n3'-UUU...GGUGU_____CC_________C__U__________G-5'\n_____________\|______________________________\|\n_____________30_____________________________1\n	275 -- 281	14 -- 20	-20.8158 kcal/mol	8.56062 kcal/mol	2.51906 kcal/mol	265 296@LB@ \| \|@LB@5'-AAA...UUGAU AA C C UU GCUUC-3'@LB@ AAAUG GGCAUUAAA AG GGC GGUGG@LB@ \|\|\|\|\| \|:\|\|\|:\|\|\| \|: :\|\| :\|\|\|\|@LB@ UUUAC CUGUAGUUU UU UCG UCACC@LB@3'-UUU...GGUGU CC C U G-5'@LB@ \| \|@LB@ 30 1@LB@@LB@@LB@Energy -9.74 kcal/mol@LB@Hybridization Energy -20.8158 kcal/mol@LB@Unfolding Energy - mRNA 8.56062 kcal/mol@LB@Unfolding Energy - sRNA 2.51906 kcal/mol@LB@@LB@Position - mRNA 266 -- 295@LB@Position - sRNA 2 -- 29@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 14 -- 20@LB@
54	0.00267	0.1735	b0131	panD	-12.96	0.079905	178 -- 195	5 -- 22	aspartate 1-decarboxylase		_____________177________________196\n_____________\|__________________\|\n5'-AUU...UUAAC____GGGU__________AAGUU...UUG-3'\n______________GACA____AGAAAGGUAG\n______________\|\|\|\|____\|\|\|\|\|:\|:\|\|\n______________CUGU____UCUUUUCGUC\n3'-UUU...UACCC____AGUU__________ACCG-5'\n_____________\|__________________\|\n_____________23_________________4\n	189 -- 195	5 -- 11	-16.4376 kcal/mol	1.9462 kcal/mol	1.53637 kcal/mol	177 196@LB@ \| \|@LB@5'-AUU...UUAAC GGGU AAGUU...UUG-3'@LB@ GACA AGAAAGGUAG@LB@ \|\|\|\| \|\|\|\|\|:\|:\|\|@LB@ CUGU UCUUUUCGUC@LB@3'-UUU...UACCC AGUU ACCG-5'@LB@ \| \|@LB@ 23 4@LB@@LB@@LB@Energy -12.96 kcal/mol@LB@Hybridization Energy -16.4376 kcal/mol@LB@Unfolding Energy - mRNA 1.9462 kcal/mol@LB@Unfolding Energy - sRNA 1.53637 kcal/mol@LB@@LB@Position - mRNA 178 -- 195@LB@Position - sRNA 5 -- 22@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 5 -- 11@LB@
10	0.0003274	0.1087	b1020	phoH	-9.93	0.281763	74 -- 85	48 -- 59	ATP-binding protein; putative PhoH family P-loop ATPase		_____________73___________86\n_____________\|____________\|\n5'-AAU...AUGUC____________UUUUA...AAC-3'\n______________ACUGAAAUGGUG\n______________\|\|:\|\|\|\|\|\|\|:\|\n______________UGGCUUUACCGC\n3'-UUU...GAACU____________CCCAA...CCG-5'\n_____________\|____________\|\n_____________60___________47\n	79 -- 85	48 -- 54	-17.134 kcal/mol	3.24305 kcal/mol	3.96136 kcal/mol	73 86@LB@ \| \|@LB@5'-AAU...AUGUC UUUUA...AAC-3'@LB@ ACUGAAAUGGUG@LB@ \|\|:\|\|\|\|\|\|\|:\|@LB@ UGGCUUUACCGC@LB@3'-UUU...GAACU CCCAA...CCG-5'@LB@ \| \|@LB@ 60 47@LB@@LB@@LB@Energy -9.93 kcal/mol@LB@Hybridization Energy -17.134 kcal/mol@LB@Unfolding Energy - mRNA 3.24305 kcal/mol@LB@Unfolding Energy - sRNA 3.96136 kcal/mol@LB@@LB@Position - mRNA 74 -- 85@LB@Position - sRNA 48 -- 59@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 48 -- 54@LB@
98	0.006107	0.2179	b3822	recQ	-9.35	0.343777	185 -- 205	1 -- 22	ATP-dependent DNA helicase		_____________184_____________________206\n_____________\|_______________________\|\n5'-CGC...UUUCA___A_______U___U_______GCAGG...UUA-3'\n______________GGC_AUCGGGG_GAA___GUGGC\n______________\|:\|_\|\|\|:::\|_:\|\|___\|\|\|\|\|\n______________CUG_UAGUUUC_UUU___CACCG\n3'-UUU...UACCC___________U___CGU_____-5'\n_____________\|______________________\|\n_____________23_____________________1\n	189 -- 195	13 -- 19	-17.8698 kcal/mol	6.98341 kcal/mol	1.54023 kcal/mol	184 206@LB@ \| \|@LB@5'-CGC...UUUCA A U U GCAGG...UUA-3'@LB@ GGC AUCGGGG GAA GUGGC@LB@ \|:\| \|\|\|:::\| :\|\| \|\|\|\|\|@LB@ CUG UAGUUUC UUU CACCG@LB@3'-UUU...UACCC U CGU -5'@LB@ \| \|@LB@ 23 1@LB@@LB@@LB@Energy -9.35 kcal/mol@LB@Hybridization Energy -17.8698 kcal/mol@LB@Unfolding Energy - mRNA 6.98341 kcal/mol@LB@Unfolding Energy - sRNA 1.54023 kcal/mol@LB@@LB@Position - mRNA 185 -- 205@LB@Position - sRNA 1 -- 22@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 13 -- 19@LB@
164	0.01206	0.2504	b0882	clpA	-9.12	0.369525	95 -- 107	48 -- 60	ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease chaperone activity		_____________94____________108\n_____________\|_____________\|\n5'-UUU...GCAGA____A________AUGGU...CGC-3'\n______________AACC_AAGUGGCG\n______________\|\|\|\|_\|\|:\|\|\|\|\|\n______________UUGG_UUUACCGC\n3'-UUU...GGAAC____C________CCCAA...CCG-5'\n_____________\|_____________\|\n_____________61____________47\n	101 -- 107	48 -- 54	-16.8445 kcal/mol	3.76103 kcal/mol	3.96136 kcal/mol	94 108@LB@ \| \|@LB@5'-UUU...GCAGA A AUGGU...CGC-3'@LB@ AACC AAGUGGCG@LB@ \|\|\|\| \|\|:\|\|\|\|\|@LB@ UUGG UUUACCGC@LB@3'-UUU...GGAAC C CCCAA...CCG-5'@LB@ \| \|@LB@ 61 47@LB@@LB@@LB@Energy -9.12 kcal/mol@LB@Hybridization Energy -16.8445 kcal/mol@LB@Unfolding Energy - mRNA 3.76103 kcal/mol@LB@Unfolding Energy - sRNA 3.96136 kcal/mol@LB@@LB@Position - mRNA 95 -- 107@LB@Position - sRNA 48 -- 60@LB@Position Seed - mRNA 101 -- 107@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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