Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
183	0.03203	0.5559	b4484	cpxP	-9.38	0.135070	107 -- 120	71 -- 85	inhibitor of the cpx response; periplasmic adaptor protein	_____________106_____________121\n_____________\|_______________\|\n5'-UAA...AUGAC_______________CACCC...GUG-3'\n______________UUUACGUUG_UUUUA\n______________\|\|:\|\|\|\|\|\|_:\|\|\|\|\n______________AAGUGCAAC_GAAAU\n3'-CGC...UUUUC_________C_____CAAUA...AAG-5'\n_____________\|_______________\|\n_____________86______________70\n	109 -- 115	77 -- 83	-11.4265 kcal/mol	1.48788 kcal/mol	0.560731 kcal/mol	106 121@LB@ \| \|@LB@5'-UAA...AUGAC CACCC...GUG-3'@LB@ UUUACGUUG UUUUA@LB@ \|\|:\|\|\|\|\|\| :\|\|\|\|@LB@ AAGUGCAAC GAAAU@LB@3'-CGC...UUUUC C CAAUA...AAG-5'@LB@ \| \|@LB@ 86 70@LB@@LB@@LB@Energy -9.38 kcal/mol@LB@Hybridization Energy -11.4265 kcal/mol@LB@Unfolding Energy - mRNA 1.48788 kcal/mol@LB@Unfolding Energy - sRNA 0.560731 kcal/mol@LB@@LB@Position - mRNA 107 -- 120@LB@Position - sRNA 71 -- 85@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 77 -- 83@LB@
98	0.0165	0.5385	b0151	fhuC	-12.15	0.044517	184 -- 202	66 -- 85	iron(3+)-hydroxamate import ABC transporter ATPase	_____________183____________________203\n_____________\|______________________\|\n5'-CUG...CCCAU_____AA_______________GCAGG...CCU-3'\n______________UUCAC__GUUGGCU___GUUAU\n______________\|\|\|\|\|__\|\|\|\|\|\|\|___\|\|\|\|\|\n______________AAGUG__CAACCGA___CAAUA\n3'-CGC...UUUUC______________AAU_____AGCUC...AAG-5'\n_____________\|______________________\|\n_____________86_____________________65\n	191 -- 197	74 -- 80	-16.912 kcal/mol	4.1925 kcal/mol	0.573112 kcal/mol	183 203@LB@ \| \|@LB@5'-CUG...CCCAU AA GCAGG...CCU-3'@LB@ UUCAC GUUGGCU GUUAU@LB@ \|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|@LB@ AAGUG CAACCGA CAAUA@LB@3'-CGC...UUUUC AAU AGCUC...AAG-5'@LB@ \| \|@LB@ 86 65@LB@@LB@@LB@Energy -12.15 kcal/mol@LB@Hybridization Energy -16.912 kcal/mol@LB@Unfolding Energy - mRNA 4.1925 kcal/mol@LB@Unfolding Energy - sRNA 0.573112 kcal/mol@LB@@LB@Position - mRNA 184 -- 202@LB@Position - sRNA 66 -- 85@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 74 -- 80@LB@
65	0.01282	0.5385	b0589	fepG	-12.08	0.045738	8 -- 17	74 -- 83	iron-enterobactin ABC transporter permease	_____________7__________18\n_____________\|__________\|\n____5'-CAUAUGG__________GGUGG...CGC-3'\n______________CGCGUUGGCU\n______________\|:\|\|\|\|\|\|\|\|\n______________GUGCAACCGA\n3'-CGC...UUCAA__________AAUCA...AAG-5'\n_____________\|__________\|\n_____________84_________73\n	11 -- 17	74 -- 80	-17.1529 kcal/mol	4.57841 kcal/mol	0.494551 kcal/mol	7 18@LB@ \| \|@LB@ 5'-CAUAUGG GGUGG...CGC-3'@LB@ CGCGUUGGCU@LB@ \|:\|\|\|\|\|\|\|\|@LB@ GUGCAACCGA@LB@3'-CGC...UUCAA AAUCA...AAG-5'@LB@ \| \|@LB@ 84 73@LB@@LB@@LB@Energy -12.08 kcal/mol@LB@Hybridization Energy -17.1529 kcal/mol@LB@Unfolding Energy - mRNA 4.57841 kcal/mol@LB@Unfolding Energy - sRNA 0.494551 kcal/mol@LB@@LB@Position - mRNA 8 -- 17@LB@Position - sRNA 74 -- 83@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 74 -- 80@LB@
43	0.009109	0.5385	b2531	iscR	-9.39	0.134324	275 -- 298	62 -- 87	isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress- and iron starvation-inducible; autorepressor	_____________274________________________299\n_____________\|__________________________\|\n5'-AUG...GGCCC___A__________GAUAUUUC____AC-3'\n______________GGU_C_CGUUGGCU_________CGA\n______________:\|\|_\|_\|\|\|\|\|\|\|\|_________\|\|\|\n______________UCA_G_GCAACCGA_________GCU\n3'-CGC...CGUUU___A_U________AAUCAAUAA___CAACU...AAG-5'\n_____________\|__________________________\|\n_____________88_________________________61\n	281 -- 287	74 -- 80	-16.3089 kcal/mol	5.20915 kcal/mol	1.70771 kcal/mol	274 299@LB@ \| \|@LB@5'-AUG...GGCCC A GAUAUUUC AC-3'@LB@ GGU C CGUUGGCU CGA@LB@ :\|\| \| \|\|\|\|\|\|\|\| \|\|\|@LB@ UCA G GCAACCGA GCU@LB@3'-CGC...CGUUU A U AAUCAAUAA CAACU...AAG-5'@LB@ \| \|@LB@ 88 61@LB@@LB@@LB@Energy -9.39 kcal/mol@LB@Hybridization Energy -16.3089 kcal/mol@LB@Unfolding Energy - mRNA 5.20915 kcal/mol@LB@Unfolding Energy - sRNA 1.70771 kcal/mol@LB@@LB@Position - mRNA 275 -- 298@LB@Position - sRNA 62 -- 87@LB@Position Seed - mRNA 281 -- 287@LB@Position Seed - sRNA 74 -- 80@LB@
57	0.01133	0.5385	b0026	ileS	-8.45	0.192830	289 -- 299	74 -- 83	isoleucyl-tRNA synthetase	_____________288_________300\n_____________\|___________\|\n5'-AUG...GCUGG________A__G-3'\n______________CGCGUUGG_CU\n______________\|:\|\|\|\|\|\|_\|\|\n______________GUGCAACC_GA\n3'-CGC...UUCAA___________AAUCA...AAG-5'\n_____________\|___________\|\n_____________84__________73\n	290 -- 296	76 -- 82	-13.1796 kcal/mol	4.23376 kcal/mol	0.494551 kcal/mol	288 300@LB@ \| \|@LB@5'-AUG...GCUGG A G-3'@LB@ CGCGUUGG CU@LB@ \|:\|\|\|\|\|\| \|\|@LB@ GUGCAACC GA@LB@3'-CGC...UUCAA AAUCA...AAG-5'@LB@ \| \|@LB@ 84 73@LB@@LB@@LB@Energy -8.45 kcal/mol@LB@Hybridization Energy -13.1796 kcal/mol@LB@Unfolding Energy - mRNA 4.23376 kcal/mol@LB@Unfolding Energy - sRNA 0.494551 kcal/mol@LB@@LB@Position - mRNA 289 -- 299@LB@Position - sRNA 74 -- 83@LB@Position Seed - mRNA 290 -- 296@LB@Position Seed - sRNA 76 -- 82@LB@
154	0.02609	0.5541	b4195	ulaC	-15.85	0.009707	6 -- 31	56 -- 80	L-ascorbate-specific enzyme IIA component of PTS	_____________5__________________________32\n_____________\|__________________________\|\n______5'-GCGCG______GAGUACAAAAG_________UGGCG...UGG-3'\n______________GUUGGC___________CGAGUUGAG\n______________\|\|\|\|\|\|___________\|\|\|\|\|\|\|\|\|\n______________CAACCG___________GCUCAACUC\n3'-CGC...AAGUG______AAAUCAAUAA__________UUUGA...AAG-5'\n_____________\|__________________________\|\n_____________81_________________________55\n	25 -- 31	56 -- 62	-23.4839 kcal/mol	5.91355 kcal/mol	1.71557 kcal/mol	5 32@LB@ \| \|@LB@ 5'-GCGCG GAGUACAAAAG UGGCG...UGG-3'@LB@ GUUGGC CGAGUUGAG@LB@ \|\|\|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ CAACCG GCUCAACUC@LB@3'-CGC...AAGUG AAAUCAAUAA UUUGA...AAG-5'@LB@ \| \|@LB@ 81 55@LB@@LB@@LB@Energy -15.85 kcal/mol@LB@Hybridization Energy -23.4839 kcal/mol@LB@Unfolding Energy - mRNA 5.91355 kcal/mol@LB@Unfolding Energy - sRNA 1.71557 kcal/mol@LB@@LB@Position - mRNA 6 -- 31@LB@Position - sRNA 56 -- 80@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 56 -- 62@LB@
134	0.0229	0.5385	b3729	glmS	-8.05	0.223933	255 -- 266	79 -- 90	L-glutamine:D-fructose-6-phosphate aminotransferase	_____________254__________267\n_____________\|____________\|\n5'-CAG...UUCUU____________CGUCU...GUC-3'\n______________GAAGGUUUACGU\n______________:\|\|:\|\|\|:\|\|\|\|\n______________UUUUCAAGUGCA\n3'-CGC...AGGCG____________ACCGA...AAG-5'\n_____________\|____________\|\n_____________91___________78\n	260 -- 266	79 -- 85	-12.2414 kcal/mol	3.68327 kcal/mol	0.506924 kcal/mol	254 267@LB@ \| \|@LB@5'-CAG...UUCUU CGUCU...GUC-3'@LB@ GAAGGUUUACGU@LB@ :\|\|:\|\|\|:\|\|\|\|@LB@ UUUUCAAGUGCA@LB@3'-CGC...AGGCG ACCGA...AAG-5'@LB@ \| \|@LB@ 91 78@LB@@LB@@LB@Energy -8.05 kcal/mol@LB@Hybridization Energy -12.2414 kcal/mol@LB@Unfolding Energy - mRNA 3.68327 kcal/mol@LB@Unfolding Energy - sRNA 0.506924 kcal/mol@LB@@LB@Position - mRNA 255 -- 266@LB@Position - sRNA 79 -- 90@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 79 -- 85@LB@
167	0.02971	0.5559	b3062	ttdB	-9.65	0.121251	224 -- 260	75 -- 106	L-tartrate dehydratase beta subunit	_____________223___________________________________261\n_____________\|_____________________________________\|\n5'-GGA...ACCCC____AAAG___A____________UA_______C___GAUGU...GCG-3'\n______________GAUC____CUG_AGAUCUGCAAGA__UUCGCGU_GGC\n______________\|\|\|\|____\|\|\|_\|\|\|\|\|:\|\|\|\|:\|__\|\|\|:\|\|\|_\|\|\|\n______________CUAG____GAC_UCUAGGCGUUUU__AAGUGCA_CCG\n________3'-CGC___________C____________C________A___AAAUC...AAG-5'\n_____________\|_____________________________________\|\n_____________107___________________________________74\n	250 -- 256	79 -- 85	-36.8095 kcal/mol	15.2774 kcal/mol	11.8787 kcal/mol	223 261@LB@ \| \|@LB@5'-GGA...ACCCC AAAG A UA C GAUGU...GCG-3'@LB@ GAUC CUG AGAUCUGCAAGA UUCGCGU GGC@LB@ \|\|\|\| \|\|\| \|\|\|\|\|:\|\|\|\|:\| \|\|\|:\|\|\| \|\|\|@LB@ CUAG GAC UCUAGGCGUUUU AAGUGCA CCG@LB@ 3'-CGC C C A AAAUC...AAG-5'@LB@ \| \|@LB@ 107 74@LB@@LB@@LB@Energy -9.65 kcal/mol@LB@Hybridization Energy -36.8095 kcal/mol@LB@Unfolding Energy - mRNA 15.2774 kcal/mol@LB@Unfolding Energy - sRNA 11.8787 kcal/mol@LB@@LB@Position - mRNA 224 -- 260@LB@Position - sRNA 75 -- 106@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 79 -- 85@LB@
99	0.01653	0.5385	b0004	thrC	-7.16	0.308279	183 -- 210	49 -- 64	L-threonine synthase	_____________182__________________________211\n_____________\|____________________________\|\n5'-CGC...GCGCA_____AC___AAAACUAAAU________ACAAU...ACG-3'\n______________CGAGU__UGG__________GAAACUCU\n______________\|\|\|\|\|__\|\|:__________\|\|\|\|\|\|\|\|\n______________GCUCA__ACU__________CUUUGAGA\n3'-CGC...AAUAA____________________________GCUUU...AAG-5'\n_____________\|____________________________\|\n_____________65___________________________48\n	204 -- 210	49 -- 55	-14.0058 kcal/mol	2.63666 kcal/mol	4.20534 kcal/mol	182 211@LB@ \| \|@LB@5'-CGC...GCGCA AC AAAACUAAAU ACAAU...ACG-3'@LB@ CGAGU UGG GAAACUCU@LB@ \|\|\|\|\| \|\|: \|\|\|\|\|\|\|\|@LB@ GCUCA ACU CUUUGAGA@LB@3'-CGC...AAUAA GCUUU...AAG-5'@LB@ \| \|@LB@ 65 48@LB@@LB@@LB@Energy -7.16 kcal/mol@LB@Hybridization Energy -14.0058 kcal/mol@LB@Unfolding Energy - mRNA 2.63666 kcal/mol@LB@Unfolding Energy - sRNA 4.20534 kcal/mol@LB@@LB@Position - mRNA 183 -- 210@LB@Position - sRNA 49 -- 64@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 49 -- 55@LB@
48	0.009918	0.5385	b0641	lptE	-9.88	0.110832	177 -- 184	74 -- 81	LPS assembly OM complex LptDE lipoprotein component	_____________176______185\n_____________\|________\|\n5'-ACG...CUGGU________AAGCG...UGA-3'\n______________CGUUGGCU\n______________\|\|\|\|\|\|\|\|\n______________GCAACCGA\n3'-CGC...CAAGU________AAUCA...AAG-5'\n_____________\|________\|\n_____________82_______73\n	178 -- 184	74 -- 80	-13.2047 kcal/mol	2.99134 kcal/mol	0.332056 kcal/mol	176 185@LB@ \| \|@LB@5'-ACG...CUGGU AAGCG...UGA-3'@LB@ CGUUGGCU@LB@ \|\|\|\|\|\|\|\|@LB@ GCAACCGA@LB@3'-CGC...CAAGU AAUCA...AAG-5'@LB@ \| \|@LB@ 82 73@LB@@LB@@LB@Energy -9.88 kcal/mol@LB@Hybridization Energy -13.2047 kcal/mol@LB@Unfolding Energy - mRNA 2.99134 kcal/mol@LB@Unfolding Energy - sRNA 0.332056 kcal/mol@LB@@LB@Position - mRNA 177 -- 184@LB@Position - sRNA 74 -- 81@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 74 -- 80@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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