Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
111	0.01894	0.5385	b2600	tyrA	-7.88	0.238290	191 -- 206	74 -- 89	fused chorismate mutase T/prephenate dehydrogenase		_____________190______________207\n_____________\|________________\|\n5'-UCG...CUUAA_________UA_____GCUGA...UGG-3'\n______________GAGGUUUAU__UGGUU\n______________:\|:\|\|\|:\|:__\|\|\|:\|\n______________UUUCAAGUG__ACCGA\n3'-CGC...GGCGU_________CA_____AAUCA...AAG-5'\n_____________\|________________\|\n_____________90_______________73\n	193 -- 199	81 -- 87	-12.4695 kcal/mol	4.06959 kcal/mol	0.517373 kcal/mol	190 207@LB@ \| \|@LB@5'-UCG...CUUAA UA GCUGA...UGG-3'@LB@ GAGGUUUAU UGGUU@LB@ :\|:\|\|\|:\|: \|\|\|:\|@LB@ UUUCAAGUG ACCGA@LB@3'-CGC...GGCGU CA AAUCA...AAG-5'@LB@ \| \|@LB@ 90 73@LB@@LB@@LB@Energy -7.88 kcal/mol@LB@Hybridization Energy -12.4695 kcal/mol@LB@Unfolding Energy - mRNA 4.06959 kcal/mol@LB@Unfolding Energy - sRNA 0.517373 kcal/mol@LB@@LB@Position - mRNA 191 -- 206@LB@Position - sRNA 74 -- 89@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 81 -- 87@LB@
88	0.01545	0.5385	b3009	yghB	-9.13	0.148475	202 -- 216	61 -- 78	general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter		_____________201________________217\n_____________\|__________________\|\n5'-UCU...GUCCA_______________A__AUAUC...UUG-3'\n______________UGGCU___GUUAUUC_AG\n______________\|\|\|\|\|___\|\|\|\|\|\|\|_\|\|\n______________ACCGA___CAAUAAG_UC\n3'-CGC...GUGCA_____AAU_______C__AACUC...AAG-5'\n_____________\|__________________\|\n_____________79_________________60\n	207 -- 213	64 -- 70	-14.1767 kcal/mol	4.24529 kcal/mol	0.796888 kcal/mol	201 217@LB@ \| \|@LB@5'-UCU...GUCCA A AUAUC...UUG-3'@LB@ UGGCU GUUAUUC AG@LB@ \|\|\|\|\| \|\|\|\|\|\|\| \|\|@LB@ ACCGA CAAUAAG UC@LB@3'-CGC...GUGCA AAU C AACUC...AAG-5'@LB@ \| \|@LB@ 79 60@LB@@LB@@LB@Energy -9.13 kcal/mol@LB@Hybridization Energy -14.1767 kcal/mol@LB@Unfolding Energy - mRNA 4.24529 kcal/mol@LB@Unfolding Energy - sRNA 0.796888 kcal/mol@LB@@LB@Position - mRNA 202 -- 216@LB@Position - sRNA 61 -- 78@LB@Position Seed - mRNA 207 -- 213@LB@Position Seed - sRNA 64 -- 70@LB@
162	0.02861	0.5559	b0564	appY	-9.53	0.127084	148 -- 162	62 -- 79	global transcriptional activator; DLP12 prophage	b1499 b3515 b4116 b0566 b3516	_____________147________________163\n_____________\|__________________\|\n5'-AAA...UUUAA__________________CUACU...UAA-3'\n______________UUGGUU___GUUAUUUGA\n______________\|\|\|\|:\|___\|\|\|\|\|\|:\|\|\n______________AACCGA___CAAUAAGCU\n3'-CGC...AGUGC______AAU_________CAACU...AAG-5'\n_____________\|__________________\|\n_____________80_________________61\n	156 -- 162	62 -- 68	-13.1987 kcal/mol	2.92728 kcal/mol	0.737706 kcal/mol	147 163@LB@ \| \|@LB@5'-AAA...UUUAA CUACU...UAA-3'@LB@ UUGGUU GUUAUUUGA@LB@ \|\|\|\|:\| \|\|\|\|\|\|:\|\|@LB@ AACCGA CAAUAAGCU@LB@3'-CGC...AGUGC AAU CAACU...AAG-5'@LB@ \| \|@LB@ 80 61@LB@@LB@@LB@Energy -9.53 kcal/mol@LB@Hybridization Energy -13.1987 kcal/mol@LB@Unfolding Energy - mRNA 2.92728 kcal/mol@LB@Unfolding Energy - sRNA 0.737706 kcal/mol@LB@@LB@Position - mRNA 148 -- 162@LB@Position - sRNA 62 -- 79@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 62 -- 68@LB@
104	0.0176	0.5385	b2788	gudX	-9.05	0.153387	171 -- 179	57 -- 65	glucarate dehydratase-related protein substrate unknown		_____________170_______180\n_____________\|_________\|\n5'-CGG...GCGUA_________AACCU...CAU-3'\n______________UCGAGUUGA\n______________\|\|\|\|\|\|\|\|\|\n______________AGCUCAACU\n3'-CGC...CAAUA_________CUUUG...AAG-5'\n_____________\|_________\|\n_____________66________56\n	173 -- 179	57 -- 63	-12.8916 kcal/mol	2.7095 kcal/mol	1.13183 kcal/mol	170 180@LB@ \| \|@LB@5'-CGG...GCGUA AACCU...CAU-3'@LB@ UCGAGUUGA@LB@ \|\|\|\|\|\|\|\|\|@LB@ AGCUCAACU@LB@3'-CGC...CAAUA CUUUG...AAG-5'@LB@ \| \|@LB@ 66 56@LB@@LB@@LB@Energy -9.05 kcal/mol@LB@Hybridization Energy -12.8916 kcal/mol@LB@Unfolding Energy - mRNA 2.7095 kcal/mol@LB@Unfolding Energy - sRNA 1.13183 kcal/mol@LB@@LB@Position - mRNA 171 -- 179@LB@Position - sRNA 57 -- 65@LB@Position Seed - mRNA 173 -- 179@LB@Position Seed - sRNA 57 -- 63@LB@
97	0.01643	0.5385	b2704	srlB	-9.20	0.144516	91 -- 103	75 -- 87	glucitol/sorbitol-specific enzyme IIA component of PTS		_____________90____________104\n_____________\|_____________\|\n5'-GGC...GACAC_________C___GUCCC...CUG-3'\n______________GGUUCGCGU_GGU\n______________:\|\|\|\|:\|\|\|_\|\|:\n______________UCAAGUGCA_CCG\n3'-CGC...CGUUU_________A___AAAUC...AAG-5'\n_____________\|_____________\|\n_____________88____________74\n	93 -- 99	79 -- 85	-16.5171 kcal/mol	6.8003 kcal/mol	0.512669 kcal/mol	90 104@LB@ \| \|@LB@5'-GGC...GACAC C GUCCC...CUG-3'@LB@ GGUUCGCGU GGU@LB@ :\|\|\|\|:\|\|\| \|\|:@LB@ UCAAGUGCA CCG@LB@3'-CGC...CGUUU A AAAUC...AAG-5'@LB@ \| \|@LB@ 88 74@LB@@LB@@LB@Energy -9.20 kcal/mol@LB@Hybridization Energy -16.5171 kcal/mol@LB@Unfolding Energy - mRNA 6.8003 kcal/mol@LB@Unfolding Energy - sRNA 0.512669 kcal/mol@LB@@LB@Position - mRNA 91 -- 103@LB@Position - sRNA 75 -- 87@LB@Position Seed - mRNA 93 -- 99@LB@Position Seed - sRNA 79 -- 85@LB@
93	0.01594	0.5385	b3430	glgC	-8.76	0.171477	231 -- 238	75 -- 82	glucose-1-phosphate adenylyltransferase		_____________230______239\n_____________\|________\|\n5'-CAG...ACUUA________GCGCC...UGA-3'\n______________AUGUUGGC\n______________\|:\|\|\|\|\|\|\n______________UGCAACCG\n3'-CGC...UCAAG________AAAUC...AAG-5'\n_____________\|________\|\n_____________83_______74\n	232 -- 238	75 -- 81	-11.9398 kcal/mol	2.84031 kcal/mol	0.339284 kcal/mol	230 239@LB@ \| \|@LB@5'-CAG...ACUUA GCGCC...UGA-3'@LB@ AUGUUGGC@LB@ \|:\|\|\|\|\|\|@LB@ UGCAACCG@LB@3'-CGC...UCAAG AAAUC...AAG-5'@LB@ \| \|@LB@ 83 74@LB@@LB@@LB@Energy -8.76 kcal/mol@LB@Hybridization Energy -11.9398 kcal/mol@LB@Unfolding Energy - mRNA 2.84031 kcal/mol@LB@Unfolding Energy - sRNA 0.339284 kcal/mol@LB@@LB@Position - mRNA 231 -- 238@LB@Position - sRNA 75 -- 82@LB@Position Seed - mRNA 232 -- 238@LB@Position Seed - sRNA 75 -- 81@LB@
184	0.03259	0.5559	b0811	glnH	-9.61	0.123139	215 -- 230	74 -- 90	glutamine transporter subunit		_____________214________________231\n_____________\|__________________\|\n5'-AUC...GUAUU_______U__________GCACU...CCG-3'\n______________AAAAGUU_CAC__UGGCU\n______________\|\|\|\|\|\|\|_\|\|\|__\|\|\|\|\|\n______________UUUUCAA_GUG__ACCGA\n3'-CGC...AGGCG___________CA_____AAUCA...AAG-5'\n_____________\|__________________\|\n_____________91_________________73\n	215 -- 221	84 -- 90	-14.2101 kcal/mol	4.07847 kcal/mol	0.517587 kcal/mol	214 231@LB@ \| \|@LB@5'-AUC...GUAUU U GCACU...CCG-3'@LB@ AAAAGUU CAC UGGCU@LB@ \|\|\|\|\|\|\| \|\|\| \|\|\|\|\|@LB@ UUUUCAA GUG ACCGA@LB@3'-CGC...AGGCG CA AAUCA...AAG-5'@LB@ \| \|@LB@ 91 73@LB@@LB@@LB@Energy -9.61 kcal/mol@LB@Hybridization Energy -14.2101 kcal/mol@LB@Unfolding Energy - mRNA 4.07847 kcal/mol@LB@Unfolding Energy - sRNA 0.517587 kcal/mol@LB@@LB@Position - mRNA 215 -- 230@LB@Position - sRNA 74 -- 90@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 84 -- 90@LB@
108	0.0187	0.5385	b1210	hemA	-9.14	0.148366	174 -- 184	75 -- 84	glutamyl tRNA reductase		_____________173_________185\n_____________\|___________\|\n5'-CCU...CCCUA__A________AUUAU...ACA-3'\n______________UC_ACGUUGGU\n______________\|\|_\|\|\|\|\|\|\|:\n______________AG_UGCAACCG\n3'-CGC...UUUCA___________AAAUC...AAG-5'\n_____________\|___________\|\n_____________85__________74\n	178 -- 184	75 -- 81	-12.935 kcal/mol	3.29275 kcal/mol	0.505918 kcal/mol	173 185@LB@ \| \|@LB@5'-CCU...CCCUA A AUUAU...ACA-3'@LB@ UC ACGUUGGU@LB@ \|\| \|\|\|\|\|\|\|:@LB@ AG UGCAACCG@LB@3'-CGC...UUUCA AAAUC...AAG-5'@LB@ \| \|@LB@ 85 74@LB@@LB@@LB@Energy -9.14 kcal/mol@LB@Hybridization Energy -12.935 kcal/mol@LB@Unfolding Energy - mRNA 3.29275 kcal/mol@LB@Unfolding Energy - sRNA 0.505918 kcal/mol@LB@@LB@Position - mRNA 174 -- 184@LB@Position - sRNA 75 -- 84@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 75 -- 81@LB@
6	0.001078	0.5385	b0849	grxA	-11.19	0.065759	209 -- 232	58 -- 81	glutaredoxin 1 redox coenzyme for ribonucleotide reductase (RNR1a)		_____________208______________________233\n_____________\|________________________\|\n5'-AGU...CAAAC____AU_______C__________CCCUU...AUC-3'\n______________CGUU__UUUUGGU_GUUCGGGUUG\n______________\|\|\|\|__:\|\|\|:\|\|_:\|\|\|\|:\|\|\|\|\n______________GCAA__GAAAUCA_UAAGCUCAAC\n3'-CGC...CAAGU____CC_______A__________UCUUU...AAG-5'\n_____________\|________________________\|\n_____________82_______________________57\n	226 -- 232	58 -- 64	-21.2762 kcal/mol	8.79419 kcal/mol	1.29619 kcal/mol	208 233@LB@ \| \|@LB@5'-AGU...CAAAC AU C CCCUU...AUC-3'@LB@ CGUU UUUUGGU GUUCGGGUUG@LB@ \|\|\|\| :\|\|\|:\|\| :\|\|\|\|:\|\|\|\|@LB@ GCAA GAAAUCA UAAGCUCAAC@LB@3'-CGC...CAAGU CC A UCUUU...AAG-5'@LB@ \| \|@LB@ 82 57@LB@@LB@@LB@Energy -11.19 kcal/mol@LB@Hybridization Energy -21.2762 kcal/mol@LB@Unfolding Energy - mRNA 8.79419 kcal/mol@LB@Unfolding Energy - sRNA 1.29619 kcal/mol@LB@@LB@Position - mRNA 209 -- 232@LB@Position - sRNA 58 -- 81@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 58 -- 64@LB@
152	0.02564	0.5485	b0887	cydD	-10.22	0.096853	263 -- 271	74 -- 82	glutathione/cysteine ABC transporter export permease/ATPase		_____________262_______272\n_____________\|_________\|\n5'-AUU...UCCCA_________GAAUA...UGA-3'\n______________ACGUUGGCU\n______________\|\|\|\|\|\|\|\|\|\n______________UGCAACCGA\n3'-CGC...UCAAG_________AAUCA...AAG-5'\n_____________\|_________\|\n_____________83________73\n	265 -- 271	74 -- 80	-15.056 kcal/mol	4.4924 kcal/mol	0.342466 kcal/mol	262 272@LB@ \| \|@LB@5'-AUU...UCCCA GAAUA...UGA-3'@LB@ ACGUUGGCU@LB@ \|\|\|\|\|\|\|\|\|@LB@ UGCAACCGA@LB@3'-CGC...UCAAG AAUCA...AAG-5'@LB@ \| \|@LB@ 83 73@LB@@LB@@LB@Energy -10.22 kcal/mol@LB@Hybridization Energy -15.056 kcal/mol@LB@Unfolding Energy - mRNA 4.4924 kcal/mol@LB@Unfolding Energy - sRNA 0.342466 kcal/mol@LB@@LB@Position - mRNA 263 -- 271@LB@Position - sRNA 74 -- 82@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 74 -- 80@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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