Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
96	0.0164	0.5385	b2670	alaE	-9.59	0.124335	245 -- 257	74 -- 86	alanine exporter alanine-inducible stress-responsive	_____________244___________258\n_____________\|_____________\|\n5'-CGG...GAUAC_______A_____GUUUA...UCU-3'\n______________GUUCGCG_UGGUU\n______________\|\|\|\|:\|\|_\|\|\|:\|\n______________CAAGUGC_ACCGA\n3'-CGC...GUUUU_______A_____AAUCA...AAG-5'\n_____________\|_____________\|\n_____________87____________73\n	245 -- 251	80 -- 86	-15.4589 kcal/mol	5.35611 kcal/mol	0.513318 kcal/mol	244 258@LB@ \| \|@LB@5'-CGG...GAUAC A GUUUA...UCU-3'@LB@ GUUCGCG UGGUU@LB@ \|\|\|\|:\|\| \|\|\|:\|@LB@ CAAGUGC ACCGA@LB@3'-CGC...GUUUU A AAUCA...AAG-5'@LB@ \| \|@LB@ 87 73@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -15.4589 kcal/mol@LB@Unfolding Energy - mRNA 5.35611 kcal/mol@LB@Unfolding Energy - sRNA 0.513318 kcal/mol@LB@@LB@Position - mRNA 245 -- 257@LB@Position - sRNA 74 -- 86@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 80 -- 86@LB@
86	0.01521	0.5385	b0605	ahpC	-11.47	0.058587	74 -- 93	73 -- 89	alkyl hydroperoxide reductase C22 subunit	_____________73___________________94\n_____________\|____________________\|\n5'-ACC...GGUGC_________AAA____U___ACGAA...GGA-3'\n______________AAAGUUCAC___GUUG_CUU\n______________\|\|\|\|\|\|\|\|\|___\|\|\|\|_\|\|\|\n______________UUUCAAGUG___CAAC_GAA\n3'-CGC...GGCGU________________C___AUCAA...AAG-5'\n_____________\|____________________\|\n_____________90___________________72\n	76 -- 82	81 -- 87	-14.6327 kcal/mol	2.64398 kcal/mol	0.517627 kcal/mol	73 94@LB@ \| \|@LB@5'-ACC...GGUGC AAA U ACGAA...GGA-3'@LB@ AAAGUUCAC GUUG CUU@LB@ \|\|\|\|\|\|\|\|\| \|\|\|\| \|\|\|@LB@ UUUCAAGUG CAAC GAA@LB@3'-CGC...GGCGU C AUCAA...AAG-5'@LB@ \| \|@LB@ 90 72@LB@@LB@@LB@Energy -11.47 kcal/mol@LB@Hybridization Energy -14.6327 kcal/mol@LB@Unfolding Energy - mRNA 2.64398 kcal/mol@LB@Unfolding Energy - sRNA 0.517627 kcal/mol@LB@@LB@Position - mRNA 74 -- 93@LB@Position - sRNA 73 -- 89@LB@Position Seed - mRNA 76 -- 82@LB@Position Seed - sRNA 81 -- 87@LB@
45	0.009513	0.5385	b2513	yfgM	-9.58	0.124672	177 -- 189	15 -- 27	ancillary SecYEG translocon subunit; putative anti-RcsB factor	_____________176___________190\n_____________\|_____________\|\n5'-GGC...UUACU_________A___AGAAG...UUG-3'\n______________GGGUUAAGG_AGG\n______________\|\|\|\|\|\|\|::_\|\|\|\n______________CCCAAUUUU_UCC\n3'-CGC...AAGUU_________C___ACGGC...AAG-5'\n_____________\|_____________\|\n_____________28____________14\n	179 -- 185	19 -- 25	-14.0675 kcal/mol	1.24912 kcal/mol	3.23583 kcal/mol	176 190@LB@ \| \|@LB@5'-GGC...UUACU A AGAAG...UUG-3'@LB@ GGGUUAAGG AGG@LB@ \|\|\|\|\|\|\|:: \|\|\|@LB@ CCCAAUUUU UCC@LB@3'-CGC...AAGUU C ACGGC...AAG-5'@LB@ \| \|@LB@ 28 14@LB@@LB@@LB@Energy -9.58 kcal/mol@LB@Hybridization Energy -14.0675 kcal/mol@LB@Unfolding Energy - mRNA 1.24912 kcal/mol@LB@Unfolding Energy - sRNA 3.23583 kcal/mol@LB@@LB@Position - mRNA 177 -- 189@LB@Position - sRNA 15 -- 27@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 19 -- 25@LB@
67	0.01284	0.5385	b1294	sapA	-9.42	0.132690	25 -- 48	61 -- 86	antimicrobial peptide transport ABC transporter periplasmic binding protein	_____________24_________________________49\n_____________\|__________________________\|\n5'-UAC...AAGUU________GA___A_______A____AAAUC...ACA-3'\n______________GUUC_UGU__GUU_UAGUUAU__GAG\n______________\|\|\|\|_:\|\|__\|:\|_\|\|\|\|\|\|\|__\|\|\|\n______________CAAG_GCA__CGA_AUCAAUA__CUC\n3'-CGC...GUUUU____U___AC___A_______AG___AACUC...AAG-5'\n_____________\|__________________________\|\n_____________87_________________________60\n	38 -- 44	66 -- 72	-17.3513 kcal/mol	6.21881 kcal/mol	1.70915 kcal/mol	24 49@LB@ \| \|@LB@5'-UAC...AAGUU GA A A AAAUC...ACA-3'@LB@ GUUC UGU GUU UAGUUAU GAG@LB@ \|\|\|\| :\|\| \|:\| \|\|\|\|\|\|\| \|\|\|@LB@ CAAG GCA CGA AUCAAUA CUC@LB@3'-CGC...GUUUU U AC A AG AACUC...AAG-5'@LB@ \| \|@LB@ 87 60@LB@@LB@@LB@Energy -9.42 kcal/mol@LB@Hybridization Energy -17.3513 kcal/mol@LB@Unfolding Energy - mRNA 6.21881 kcal/mol@LB@Unfolding Energy - sRNA 1.70915 kcal/mol@LB@@LB@Position - mRNA 25 -- 48@LB@Position - sRNA 61 -- 86@LB@Position Seed - mRNA 38 -- 44@LB@Position Seed - sRNA 66 -- 72@LB@
163	0.02879	0.5559	b4244	pyrI	-5.82	0.477897	276 -- 293	60 -- 77	aspartate carbamoyltransferase regulatory subunit	_____________275________________294\n_____________\|__________________\|\n5'-UUC...AGAUC_______AGCUG______CUGUUCA-3'\n______________GGUUUUA_____UUGAGU\n______________\|\|:\|\|\|\|_____\|:\|\|\|\|\n______________CCGAAAU_____AGCUCA\n3'-CGC...UGCAA_______CAAUA______ACUCU...AAG-5'\n_____________\|__________________\|\n_____________78_________________59\n	276 -- 282	71 -- 77	-12.7499 kcal/mol	6.13118 kcal/mol	0.796465 kcal/mol	275 294@LB@ \| \|@LB@5'-UUC...AGAUC AGCUG CUGUUCA-3'@LB@ GGUUUUA UUGAGU@LB@ \|\|:\|\|\|\| \|:\|\|\|\|@LB@ CCGAAAU AGCUCA@LB@3'-CGC...UGCAA CAAUA ACUCU...AAG-5'@LB@ \| \|@LB@ 78 59@LB@@LB@@LB@Energy -5.82 kcal/mol@LB@Hybridization Energy -12.7499 kcal/mol@LB@Unfolding Energy - mRNA 6.13118 kcal/mol@LB@Unfolding Energy - sRNA 0.796465 kcal/mol@LB@@LB@Position - mRNA 276 -- 293@LB@Position - sRNA 60 -- 77@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 71 -- 77@LB@
29	0.005218	0.5385	b3433	asd	-11.22	0.064904	208 -- 219	71 -- 82	aspartate-semialdehyde dehydrogenase NAD(P)-binding	_____________207__________220\n_____________\|____________\|\n5'-UCA...GAAAA____________UCGGC...UCU-3'\n______________AUGUUGGUUUUA\n______________\|:\|\|\|\|\|:\|\|\|\|\n______________UGCAACCGAAAU\n3'-CGC...UCAAG____________CAAUA...AAG-5'\n_____________\|____________\|\n_____________83___________70\n	213 -- 219	71 -- 77	-13.9075 kcal/mol	2.30708 kcal/mol	0.38228 kcal/mol	207 220@LB@ \| \|@LB@5'-UCA...GAAAA UCGGC...UCU-3'@LB@ AUGUUGGUUUUA@LB@ \|:\|\|\|\|\|:\|\|\|\|@LB@ UGCAACCGAAAU@LB@3'-CGC...UCAAG CAAUA...AAG-5'@LB@ \| \|@LB@ 83 70@LB@@LB@@LB@Energy -11.22 kcal/mol@LB@Hybridization Energy -13.9075 kcal/mol@LB@Unfolding Energy - mRNA 2.30708 kcal/mol@LB@Unfolding Energy - sRNA 0.38228 kcal/mol@LB@@LB@Position - mRNA 208 -- 219@LB@Position - sRNA 71 -- 82@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 71 -- 77@LB@
187	0.03332	0.5559	b2019	hisG	-10.76	0.077976	170 -- 191	15 -- 34	ATP phosphoribosyltransferase	_____________169____________________192\n_____________\|______________________\|\n5'-UUU...AUAAC_G_____ACA____U_______AAUAA...AUC-3'\n______________G_UUCAG___GGUU_AAAGAGG\n______________:_\|\|\|\|:___\|\|\|\|_\|\|\|\|\|\|\|\n______________U_AAGUU___CCAA_UUUCUCC\n3'-CGC...GUCAC_G_____C______U_______ACGGC...AAG-5'\n_____________\|______________________\|\n_____________35_____________________14\n	185 -- 191	15 -- 21	-17.0816 kcal/mol	2.9882 kcal/mol	3.33044 kcal/mol	169 192@LB@ \| \|@LB@5'-UUU...AUAAC G ACA U AAUAA...AUC-3'@LB@ G UUCAG GGUU AAAGAGG@LB@ : \|\|\|\|: \|\|\|\| \|\|\|\|\|\|\|@LB@ U AAGUU CCAA UUUCUCC@LB@3'-CGC...GUCAC G C U ACGGC...AAG-5'@LB@ \| \|@LB@ 35 14@LB@@LB@@LB@Energy -10.76 kcal/mol@LB@Hybridization Energy -17.0816 kcal/mol@LB@Unfolding Energy - mRNA 2.9882 kcal/mol@LB@Unfolding Energy - sRNA 3.33044 kcal/mol@LB@@LB@Position - mRNA 170 -- 191@LB@Position - sRNA 15 -- 34@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 15 -- 21@LB@
19	0.003195	0.5385	b3739	atpI	-12.12	0.045044	59 -- 85	62 -- 85	ATP synthase membrane-bound accessory factor	_____________58___________________________86\n_____________\|____________________________\|\n5'-GAA...AUUUA_____CUU______AC____________AAUCA...ACC-3'\n______________UUCAC___UUGGCU__UUA_UUGUUUGA\n______________\|\|\|\|\|___\|\|\|\|\|\|__\|\|\|_\|\|:\|\|:\|\|\n______________AAGUG___AACCGA__AAU_AAUAAGCU\n3'-CGC...UUUUC_____C_____________C________CAACU...AAG-5'\n_____________\|____________________________\|\n_____________86___________________________61\n	79 -- 85	62 -- 68	-17.7596 kcal/mol	3.93623 kcal/mol	1.70582 kcal/mol	58 86@LB@ \| \|@LB@5'-GAA...AUUUA CUU AC AAUCA...ACC-3'@LB@ UUCAC UUGGCU UUA UUGUUUGA@LB@ \|\|\|\|\| \|\|\|\|\|\| \|\|\| \|\|:\|\|:\|\|@LB@ AAGUG AACCGA AAU AAUAAGCU@LB@3'-CGC...UUUUC C C CAACU...AAG-5'@LB@ \| \|@LB@ 86 61@LB@@LB@@LB@Energy -12.12 kcal/mol@LB@Hybridization Energy -17.7596 kcal/mol@LB@Unfolding Energy - mRNA 3.93623 kcal/mol@LB@Unfolding Energy - sRNA 1.70582 kcal/mol@LB@@LB@Position - mRNA 59 -- 85@LB@Position - sRNA 62 -- 85@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 62 -- 68@LB@
85	0.01519	0.5385	b0094	ftsA	-12.00	0.047343	234 -- 271	69 -- 107	ATP-binding cell division FtsK recruitment protein	_____________233__________________________________________272\n_____________\|____________________________________________\|\n5'-AAU...UAGUA_____________UGGUA___________GC_____________AGGGG...UCA-3'\n______________GGA__CUGGAGAU_____CCGCGAAGGUU__C____GCUUUAGU\n______________\|\|\|__\|\|\|\|\|\|\|\|_____\|\|\|\|:\|\|:\|\|\|__\|____\|\|\|\|\|\|\|\|\n______________CCU__GACCUCUA_____GGCGUUUUCAA__G____CGAAAUCA\n_________3'-CG___AG________________________GU_CAAC________AUAAG...AAG-5'\n_____________\|____________________________________________\|\n_____________108__________________________________________68\n	265 -- 271	69 -- 75	-38.6824 kcal/mol	14.7528 kcal/mol	11.9342 kcal/mol	233 272@LB@ \| \|@LB@5'-AAU...UAGUA UGGUA GC AGGGG...UCA-3'@LB@ GGA CUGGAGAU CCGCGAAGGUU C GCUUUAGU@LB@ \|\|\| \|\|\|\|\|\|\|\| \|\|\|\|:\|\|:\|\|\| \| \|\|\|\|\|\|\|\|@LB@ CCU GACCUCUA GGCGUUUUCAA G CGAAAUCA@LB@ 3'-CG AG GU CAAC AUAAG...AAG-5'@LB@ \| \|@LB@ 108 68@LB@@LB@@LB@Energy -12.00 kcal/mol@LB@Hybridization Energy -38.6824 kcal/mol@LB@Unfolding Energy - mRNA 14.7528 kcal/mol@LB@Unfolding Energy - sRNA 11.9342 kcal/mol@LB@@LB@Position - mRNA 234 -- 271@LB@Position - sRNA 69 -- 107@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 69 -- 75@LB@
145	0.02443	0.5467	b2569	lepA	-12.53	0.038132	5 -- 17	71 -- 85	back-translocating elongation factor EF4 GTPase	_____________4_______________18\n_____________\|_______________\|\n_______5'-UGAU_______________UUGCA...ACC-3'\n______________UUCAC__UGGCUUUG\n______________\|\|\|\|\|__\|\|\|\|\|\|\|:\n______________AAGUG__ACCGAAAU\n3'-CGC...UUUUC_____CA________CAAUA...AAG-5'\n_____________\|_______________\|\n_____________86______________70\n	11 -- 17	71 -- 77	-14.3569 kcal/mol	1.27074 kcal/mol	0.560731 kcal/mol	4 18@LB@ \| \|@LB@ 5'-UGAU UUGCA...ACC-3'@LB@ UUCAC UGGCUUUG@LB@ \|\|\|\|\| \|\|\|\|\|\|\|:@LB@ AAGUG ACCGAAAU@LB@3'-CGC...UUUUC CA CAAUA...AAG-5'@LB@ \| \|@LB@ 86 70@LB@@LB@@LB@Energy -12.53 kcal/mol@LB@Hybridization Energy -14.3569 kcal/mol@LB@Unfolding Energy - mRNA 1.27074 kcal/mol@LB@Unfolding Energy - sRNA 0.560731 kcal/mol@LB@@LB@Position - mRNA 5 -- 17@LB@Position - sRNA 71 -- 85@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 71 -- 77@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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