Input and runtime details for job 3429477 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3429477 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:26:36 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:01 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:15:20 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:15:27 CET 2018
	Job Completed		@ Fri Feb 16 20:15:52 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3429477&toolName=CopraRNA ( 30 days expiry )

Description of the job

OxyS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
41	0.00828	0.5385	b2246	rhmT	-13.65	0.023999	257 -- 273	69 -- 86	putative L-rhamnonate transporter	_____________256________________274\n_____________\|__________________\|\n5'-ACG...AUUCC____________GCU___AUGUG...CGA-3'\n______________GUUUAUGUUGGC____GU\n______________\|\|\|:\|:\|\|\|\|\|\|____\|\|\n______________CAAGUGCAACCG____CA\n3'-CGC...GUUUU____________AAAU__AUAAG...AAG-5'\n_____________\|__________________\|\n_____________87_________________68\n	262 -- 268	75 -- 81	-18.6828 kcal/mol	4.45929 kcal/mol	0.568509 kcal/mol	256 274@LB@ \| \|@LB@5'-ACG...AUUCC GCU AUGUG...CGA-3'@LB@ GUUUAUGUUGGC GU@LB@ \|\|\|:\|:\|\|\|\|\|\| \|\|@LB@ CAAGUGCAACCG CA@LB@3'-CGC...GUUUU AAAU AUAAG...AAG-5'@LB@ \| \|@LB@ 87 68@LB@@LB@@LB@Energy -13.65 kcal/mol@LB@Hybridization Energy -18.6828 kcal/mol@LB@Unfolding Energy - mRNA 4.45929 kcal/mol@LB@Unfolding Energy - sRNA 0.568509 kcal/mol@LB@@LB@Position - mRNA 257 -- 273@LB@Position - sRNA 69 -- 86@LB@Position Seed - mRNA 262 -- 268@LB@Position Seed - sRNA 75 -- 81@LB@
195	0.03428	0.5559	b0434	yajG	-10.30	0.093744	131 -- 149	66 -- 84	putative lipoprotein	_____________130__________________150\n_____________\|____________________\|\n5'-UGG...UCCCC___C_______AAU______GAUGG...UGC-3'\n______________UCG_CGUUGGC____GUUAU\n______________\|\|:_\|\|\|\|\|\|\|____\|\|\|\|\|\n______________AGU_GCAACCG____CAAUA\n3'-CGC...UUUCA___________AAAU_____AGCUC...AAG-5'\n_____________\|____________________\|\n_____________85___________________65\n	135 -- 141	75 -- 81	-17.0927 kcal/mol	6.21917 kcal/mol	0.5705 kcal/mol	130 150@LB@ \| \|@LB@5'-UGG...UCCCC C AAU GAUGG...UGC-3'@LB@ UCG CGUUGGC GUUAU@LB@ \|\|: \|\|\|\|\|\|\| \|\|\|\|\|@LB@ AGU GCAACCG CAAUA@LB@3'-CGC...UUUCA AAAU AGCUC...AAG-5'@LB@ \| \|@LB@ 85 65@LB@@LB@@LB@Energy -10.30 kcal/mol@LB@Hybridization Energy -17.0927 kcal/mol@LB@Unfolding Energy - mRNA 6.21917 kcal/mol@LB@Unfolding Energy - sRNA 0.5705 kcal/mol@LB@@LB@Position - mRNA 131 -- 149@LB@Position - sRNA 66 -- 84@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 75 -- 81@LB@
171	0.03054	0.5559	b0045	yaaU	-10.82	0.076059	142 -- 153	73 -- 84	putative MFS sugar transporter; membrane protein	_____________141__________154\n_____________\|____________\|\n5'-AAG...ACGGC_G__________GAUAC...UAC-3'\n______________U_AUGUUGGUUU\n______________\|_\|:\|\|\|\|\|:\|\|\n______________A_UGCAACCGAA\n3'-CGC...UUUCA_G__________AUCAA...AAG-5'\n_____________\|____________\|\n_____________85___________72\n	147 -- 153	73 -- 79	-12.9415 kcal/mol	1.60777 kcal/mol	0.508835 kcal/mol	141 154@LB@ \| \|@LB@5'-AAG...ACGGC G GAUAC...UAC-3'@LB@ U AUGUUGGUUU@LB@ \| \|:\|\|\|\|\|:\|\|@LB@ A UGCAACCGAA@LB@3'-CGC...UUUCA G AUCAA...AAG-5'@LB@ \| \|@LB@ 85 72@LB@@LB@@LB@Energy -10.82 kcal/mol@LB@Hybridization Energy -12.9415 kcal/mol@LB@Unfolding Energy - mRNA 1.60777 kcal/mol@LB@Unfolding Energy - sRNA 0.508835 kcal/mol@LB@@LB@Position - mRNA 142 -- 153@LB@Position - sRNA 73 -- 84@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 73 -- 79@LB@
136	0.02309	0.5385	b3223	nanE	-8.63	0.180292	34 -- 47	74 -- 86	putative N-acetylmannosamine-6-P epimerase	_____________33_____________48\n_____________\|______________\|\n5'-AUC...CUCGC_____G________GCGCC...AAA-3'\n______________GUUCA_CGUUGGUU\n______________\|\|\|\|\|_\|\|\|\|\|\|:\|\n______________CAAGU_GCAACCGA\n3'-CGC...GUUUU______________AAUCA...AAG-5'\n_____________\|______________\|\n_____________87_____________73\n	41 -- 47	74 -- 80	-15.9355 kcal/mol	6.79344 kcal/mol	0.513318 kcal/mol	33 48@LB@ \| \|@LB@5'-AUC...CUCGC G GCGCC...AAA-3'@LB@ GUUCA CGUUGGUU@LB@ \|\|\|\|\| \|\|\|\|\|\|:\|@LB@ CAAGU GCAACCGA@LB@3'-CGC...GUUUU AAUCA...AAG-5'@LB@ \| \|@LB@ 87 73@LB@@LB@@LB@Energy -8.63 kcal/mol@LB@Hybridization Energy -15.9355 kcal/mol@LB@Unfolding Energy - mRNA 6.79344 kcal/mol@LB@Unfolding Energy - sRNA 0.513318 kcal/mol@LB@@LB@Position - mRNA 34 -- 47@LB@Position - sRNA 74 -- 86@LB@Position Seed - mRNA 41 -- 47@LB@Position Seed - sRNA 74 -- 80@LB@
172	0.03064	0.5559	b1542	ydfI	-13.08	0.030437	273 -- 290	69 -- 86	putative NAD-dependent D-mannonate oxidoreductase	_____________272________________291\n_____________\|__________________\|\n5'-CUC...GAAUU______UUA_________GCAUU...ACC-3'\n______________GUUCAU___GGCUUUGGU\n______________\|\|\|\|\|:___\|\|\|\|\|\|:\|\|\n______________CAAGUG___CCGAAAUCA\n3'-CGC...GUUUU______CAA_________AUAAG...AAG-5'\n_____________\|__________________\|\n_____________87_________________68\n	284 -- 290	69 -- 75	-19.6529 kcal/mol	6.00837 kcal/mol	0.568509 kcal/mol	272 291@LB@ \| \|@LB@5'-CUC...GAAUU UUA GCAUU...ACC-3'@LB@ GUUCAU GGCUUUGGU@LB@ \|\|\|\|\|: \|\|\|\|\|\|:\|\|@LB@ CAAGUG CCGAAAUCA@LB@3'-CGC...GUUUU CAA AUAAG...AAG-5'@LB@ \| \|@LB@ 87 68@LB@@LB@@LB@Energy -13.08 kcal/mol@LB@Hybridization Energy -19.6529 kcal/mol@LB@Unfolding Energy - mRNA 6.00837 kcal/mol@LB@Unfolding Energy - sRNA 0.568509 kcal/mol@LB@@LB@Position - mRNA 273 -- 290@LB@Position - sRNA 69 -- 86@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 69 -- 75@LB@
189	0.03342	0.5559	b2172	yeiQ	-17.65	0.004645	282 -- 298	70 -- 86	putative NAD-dependent D-mannonate oxidoreductase	_____________281_______________299\n_____________\|_________________\|\n5'-GAU...GUAUC______U__________AG-3'\n______________GUUCAU_UUGGCUUUGG\n______________\|\|\|\|\|:_\|\|\|\|\|\|\|\|:\|\n______________CAAGUG_AACCGAAAUC\n3'-CGC...GUUUU______C__________AAUAA...AAG-5'\n_____________\|_________________\|\n_____________87________________69\n	292 -- 298	70 -- 76	-21.6632 kcal/mol	3.44897 kcal/mol	0.568254 kcal/mol	281 299@LB@ \| \|@LB@5'-GAU...GUAUC U AG-3'@LB@ GUUCAU UUGGCUUUGG@LB@ \|\|\|\|\|: \|\|\|\|\|\|\|\|:\|@LB@ CAAGUG AACCGAAAUC@LB@3'-CGC...GUUUU C AAUAA...AAG-5'@LB@ \| \|@LB@ 87 69@LB@@LB@@LB@Energy -17.65 kcal/mol@LB@Hybridization Energy -21.6632 kcal/mol@LB@Unfolding Energy - mRNA 3.44897 kcal/mol@LB@Unfolding Energy - sRNA 0.568254 kcal/mol@LB@@LB@Position - mRNA 282 -- 298@LB@Position - sRNA 70 -- 86@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 70 -- 76@LB@
30	0.005373	0.5385	b1624	ydgJ	-10.76	0.078067	229 -- 247	61 -- 79	putative oxidoreductase	_____________228_________________248\n_____________\|___________________\|\n5'-GUA...GUUGA____G_A_______G____CAAAA...CGG-3'\n______________UUGG_U_UGGUUAU_CGAG\n______________\|\|\|\|_\|_\|:\|\|\|\|\|_\|\|\|\|\n______________AACC_A_AUCAAUA_GCUC\n3'-CGC...AGUGC____G_A_______A____AACUC...AAG-5'\n_____________\|___________________\|\n_____________80__________________60\n	236 -- 242	66 -- 72	-16.8363 kcal/mol	5.27817 kcal/mol	0.798019 kcal/mol	228 248@LB@ \| \|@LB@5'-GUA...GUUGA G A G CAAAA...CGG-3'@LB@ UUGG U UGGUUAU CGAG@LB@ \|\|\|\| \| \|:\|\|\|\|\| \|\|\|\|@LB@ AACC A AUCAAUA GCUC@LB@3'-CGC...AGUGC G A A AACUC...AAG-5'@LB@ \| \|@LB@ 80 60@LB@@LB@@LB@Energy -10.76 kcal/mol@LB@Hybridization Energy -16.8363 kcal/mol@LB@Unfolding Energy - mRNA 5.27817 kcal/mol@LB@Unfolding Energy - sRNA 0.798019 kcal/mol@LB@@LB@Position - mRNA 229 -- 247@LB@Position - sRNA 61 -- 79@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 66 -- 72@LB@
173	0.03066	0.5559	b2928	yggC	-11.87	0.049837	290 -- 298	75 -- 83	putative PanK family P-loop kinase	_____________289_______299\n_____________\|_________\|\n5'-GAA...CUGCA_________GG-3'\n______________CAUGUUGGC\n______________\|\|:\|\|\|\|\|\|\n______________GUGCAACCG\n3'-CGC...UUCAA_________AAAUC...AAG-5'\n_____________\|_________\|\n_____________84________74\n	292 -- 298	75 -- 81	-14.124 kcal/mol	1.76242 kcal/mol	0.492259 kcal/mol	289 299@LB@ \| \|@LB@5'-GAA...CUGCA GG-3'@LB@ CAUGUUGGC@LB@ \|\|:\|\|\|\|\|\|@LB@ GUGCAACCG@LB@3'-CGC...UUCAA AAAUC...AAG-5'@LB@ \| \|@LB@ 84 74@LB@@LB@@LB@Energy -11.87 kcal/mol@LB@Hybridization Energy -14.124 kcal/mol@LB@Unfolding Energy - mRNA 1.76242 kcal/mol@LB@Unfolding Energy - sRNA 0.492259 kcal/mol@LB@@LB@Position - mRNA 290 -- 298@LB@Position - sRNA 75 -- 83@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 75 -- 81@LB@
82	0.01477	0.5385	b3527	yhjJ	-9.01	0.155560	190 -- 221	62 -- 91	putative periplasmic M16 family chaperone	_____________189_______________________________222\n_____________\|_________________________________\|\n5'-CUC...UUGAU___________AUGCAG___ACAAAA_______CUUUU...GGC-3'\n______________CAGGGGUUCAC______GGC_______AUUCGA\n______________\|\|:::\|\|\|\|\|\|______\|\|\|_______\|\|\|\|\|\|\n______________GUUUUCAAGUG______CCG_______UAAGCU\n3'-CGC...UAGGC___________CAA______AAAUCAA______CAACU...AAG-5'\n_____________\|_________________________________\|\n_____________92________________________________61\n	194 -- 200	81 -- 87	-19.9715 kcal/mol	6.94165 kcal/mol	4.01597 kcal/mol	189 222@LB@ \| \|@LB@5'-CUC...UUGAU AUGCAG ACAAAA CUUUU...GGC-3'@LB@ CAGGGGUUCAC GGC AUUCGA@LB@ \|\|:::\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|@LB@ GUUUUCAAGUG CCG UAAGCU@LB@3'-CGC...UAGGC CAA AAAUCAA CAACU...AAG-5'@LB@ \| \|@LB@ 92 61@LB@@LB@@LB@Energy -9.01 kcal/mol@LB@Hybridization Energy -19.9715 kcal/mol@LB@Unfolding Energy - mRNA 6.94165 kcal/mol@LB@Unfolding Energy - sRNA 4.01597 kcal/mol@LB@@LB@Position - mRNA 190 -- 221@LB@Position - sRNA 62 -- 91@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 81 -- 87@LB@
68	0.013	0.5385	b2823	ppdC	-12.74	0.034901	243 -- 262	69 -- 89	putative prepilin peptidase-dependent protein	_____________242___________________263\n_____________\|_____________________\|\n5'-ACG...UGCCG_____A_________GA____GUUGA...UCC-3'\n______________GAGGU__AUGUUGGC__UGGU\n______________:\|:\|\|__\|:\|\|\|\|\|\|__\|:\|\|\n______________UUUCA__UGCAACCG__AUCA\n3'-CGC...GGCGU_____AG________AA____AUAAG...AAG-5'\n_____________\|_____________________\|\n_____________90____________________68\n	250 -- 256	75 -- 81	-18.5568 kcal/mol	5.24273 kcal/mol	0.572143 kcal/mol	242 263@LB@ \| \|@LB@5'-ACG...UGCCG A GA GUUGA...UCC-3'@LB@ GAGGU AUGUUGGC UGGU@LB@ :\|:\|\| \|:\|\|\|\|\|\| \|:\|\|@LB@ UUUCA UGCAACCG AUCA@LB@3'-CGC...GGCGU AG AA AUAAG...AAG-5'@LB@ \| \|@LB@ 90 68@LB@@LB@@LB@Energy -12.74 kcal/mol@LB@Hybridization Energy -18.5568 kcal/mol@LB@Unfolding Energy - mRNA 5.24273 kcal/mol@LB@Unfolding Energy - sRNA 0.572143 kcal/mol@LB@@LB@Position - mRNA 243 -- 262@LB@Position - sRNA 69 -- 89@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 75 -- 81@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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