Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
162	0.03332	0.6177	b3304	rplR	-8.58	0.079933	170 -- 177	46 -- 53	50S ribosomal subunit protein L18	_____________169______178\n_____________\|________\|\n5'-AUC...CGUAC________CUAAG...GUC-3'\n______________CAAAGAGG\n______________\|\|\|\|\|\|\|\|\n______________GUUUCUCC\n3'-UUU...GGGCA________UUAAA...ACA-5'\n_____________\|________\|\n_____________54_______45\n	171 -- 177	46 -- 52	-11.2869 kcal/mol	1.80103 kcal/mol	0.910679 kcal/mol	169 178@LB@ \| \|@LB@5'-AUC...CGUAC CUAAG...GUC-3'@LB@ CAAAGAGG@LB@ \|\|\|\|\|\|\|\|@LB@ GUUUCUCC@LB@3'-UUU...GGGCA UUAAA...ACA-5'@LB@ \| \|@LB@ 54 45@LB@@LB@@LB@Energy -8.58 kcal/mol@LB@Hybridization Energy -11.2869 kcal/mol@LB@Unfolding Energy - mRNA 1.80103 kcal/mol@LB@Unfolding Energy - sRNA 0.910679 kcal/mol@LB@@LB@Position - mRNA 170 -- 177@LB@Position - sRNA 46 -- 53@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 46 -- 52@LB@
80	0.01709	0.6177	b2498	upp	-4.70	0.418803	173 -- 195	44 -- 70	uracil phosphoribosyltransferase	_____________172_________________________196\n_____________\|___________________________\|\n5'-GGC...UUUUU___G___________C_______A___AGAGU...CCG-3'\n______________UGU_GCU____GCCC_UCAAAGG_GAA\n______________\|:\|_\|\|\|____\|\|\|\|_\|\|\|\|\|\|:_\|\|\|\n______________AUA_CGA____CGGG_AGUUUCU_CUU\n3'-UUU...CGGUU___G___UAAC____C_______C___AAAGU...ACA-5'\n_____________\|___________________________\|\n_____________71__________________________43\n	185 -- 191	48 -- 54	-22.3772 kcal/mol	6.37003 kcal/mol	11.3101 kcal/mol	172 196@LB@ \| \|@LB@5'-GGC...UUUUU G C A AGAGU...CCG-3'@LB@ UGU GCU GCCC UCAAAGG GAA@LB@ \|:\| \|\|\| \|\|\|\| \|\|\|\|\|\|: \|\|\|@LB@ AUA CGA CGGG AGUUUCU CUU@LB@3'-UUU...CGGUU G UAAC C C AAAGU...ACA-5'@LB@ \| \|@LB@ 71 43@LB@@LB@@LB@Energy -4.70 kcal/mol@LB@Hybridization Energy -22.3772 kcal/mol@LB@Unfolding Energy - mRNA 6.37003 kcal/mol@LB@Unfolding Energy - sRNA 11.3101 kcal/mol@LB@@LB@Position - mRNA 173 -- 195@LB@Position - sRNA 44 -- 70@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 48 -- 54@LB@
72	0.015	0.6177	b2513	yfgM	-10.40	0.034106	179 -- 190	45 -- 55	ancillary SecYEG translocon subunit; putative anti-RcsB factor	_____________178__________191\n_____________\|____________\|\n5'-GGC...ACUGG________A___GAAGG...UUG-3'\n______________GUUAAGGA_GGA\n______________\|\|:\|\|:\|\|_\|\|\|\n______________CAGUUUCU_CCU\n3'-UUU...CCGGG____________UAAAG...ACA-5'\n_____________\|____________\|\n_____________56___________44\n	180 -- 186	48 -- 54	-12.2699 kcal/mol	0.940443 kcal/mol	0.930875 kcal/mol	178 191@LB@ \| \|@LB@5'-GGC...ACUGG A GAAGG...UUG-3'@LB@ GUUAAGGA GGA@LB@ \|\|:\|\|:\|\| \|\|\|@LB@ CAGUUUCU CCU@LB@3'-UUU...CCGGG UAAAG...ACA-5'@LB@ \| \|@LB@ 56 44@LB@@LB@@LB@Energy -10.40 kcal/mol@LB@Hybridization Energy -12.2699 kcal/mol@LB@Unfolding Energy - mRNA 0.940443 kcal/mol@LB@Unfolding Energy - sRNA 0.930875 kcal/mol@LB@@LB@Position - mRNA 179 -- 190@LB@Position - sRNA 45 -- 55@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 48 -- 54@LB@
110	0.02234	0.6177	b0141	yadN	-8.72	0.074917	179 -- 189	37 -- 47	putative fimbrial-like adhesin protein	_____________178_________190\n_____________\|___________\|\n5'-UUA...CUAAU___________AAAGG...GUG-3'\n______________GGAAUUUUAUU\n______________\|\|\|\|\|\|\|:\|\|\|\n______________CCUUAAAGUAA\n3'-UUU...UUUCU___________AAAAA...ACA-5'\n_____________\|___________\|\n_____________48__________36\n	183 -- 189	37 -- 43	-11.1734 kcal/mol	1.41261 kcal/mol	1.04525 kcal/mol	178 190@LB@ \| \|@LB@5'-UUA...CUAAU AAAGG...GUG-3'@LB@ GGAAUUUUAUU@LB@ \|\|\|\|\|\|\|:\|\|\|@LB@ CCUUAAAGUAA@LB@3'-UUU...UUUCU AAAAA...ACA-5'@LB@ \| \|@LB@ 48 36@LB@@LB@@LB@Energy -8.72 kcal/mol@LB@Hybridization Energy -11.1734 kcal/mol@LB@Unfolding Energy - mRNA 1.41261 kcal/mol@LB@Unfolding Energy - sRNA 1.04525 kcal/mol@LB@@LB@Position - mRNA 179 -- 189@LB@Position - sRNA 37 -- 47@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 37 -- 43@LB@
115	0.02287	0.6177	b3621	waaC	-10.02	0.040839	179 -- 191	44 -- 55	ADP-heptose:LPS heptosyl transferase I	_____________178___________192\n_____________\|_____________\|\n5'-UUG...CUAUU____C________GCCUG...UUG-3'\n______________GUUA_AAGAGGAA\n______________\|\|:\|_\|\|\|\|\|\|\|\|\n______________CAGU_UUCUCCUU\n3'-UUU...CCGGG_____________AAAGU...ACA-5'\n_____________\|_____________\|\n_____________56____________43\n	185 -- 191	44 -- 50	-12.6771 kcal/mol	1.72007 kcal/mol	0.941432 kcal/mol	178 192@LB@ \| \|@LB@5'-UUG...CUAUU C GCCUG...UUG-3'@LB@ GUUA AAGAGGAA@LB@ \|\|:\| \|\|\|\|\|\|\|\|@LB@ CAGU UUCUCCUU@LB@3'-UUU...CCGGG AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -10.02 kcal/mol@LB@Hybridization Energy -12.6771 kcal/mol@LB@Unfolding Energy - mRNA 1.72007 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 179 -- 191@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 44 -- 50@LB@
133	0.02604	0.6177	b0876	ybjD	-5.23	0.342712	179 -- 199	45 -- 55	putative OLD family ATP-dependent endonuclease; DUF2813 family protein	_____________178___________________200\n_____________\|_____________________\|\n5'-CGU...GAUAA____CAUAUUUCUG_______UAUGA...GGG-3'\n______________GUCA__________GAGGGGA\n______________\|\|\|\|__________:\|\|:\|\|\|\n______________CAGU__________UUCUCCU\n3'-UUU...CCGGG_____________________UAAAG...ACA-5'\n_____________\|_____________________\|\n_____________56____________________44\n	193 -- 199	45 -- 51	-10.7419 kcal/mol	4.58374 kcal/mol	0.930875 kcal/mol	178 200@LB@ \| \|@LB@5'-CGU...GAUAA CAUAUUUCUG UAUGA...GGG-3'@LB@ GUCA GAGGGGA@LB@ \|\|\|\| :\|\|:\|\|\|@LB@ CAGU UUCUCCU@LB@3'-UUU...CCGGG UAAAG...ACA-5'@LB@ \| \|@LB@ 56 44@LB@@LB@@LB@Energy -5.23 kcal/mol@LB@Hybridization Energy -10.7419 kcal/mol@LB@Unfolding Energy - mRNA 4.58374 kcal/mol@LB@Unfolding Energy - sRNA 0.930875 kcal/mol@LB@@LB@Position - mRNA 179 -- 199@LB@Position - sRNA 45 -- 55@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 45 -- 51@LB@
192	0.04008	0.6177	b2822	recC	-5.02	0.370915	179 -- 195	44 -- 57	exonuclease V (RecBCD complex) gamma chain	_____________178_______________196\n_____________\|_________________\|\n5'-AGU...CCGAA_______U__UC_____CCGCU...AGA-3'\n______________UCGUCAG_AG__AGGAG\n______________:\|\|\|\|\|:_\|\|__\|\|\|\|:\n______________GGCAGUU_UC__UCCUU\n3'-UUU...AACCG_________________AAAGU...ACA-5'\n_____________\|_________________\|\n_____________58________________43\n	179 -- 185	51 -- 57	-11.1502 kcal/mol	3.39036 kcal/mol	2.73756 kcal/mol	178 196@LB@ \| \|@LB@5'-AGU...CCGAA U UC CCGCU...AGA-3'@LB@ UCGUCAG AG AGGAG@LB@ :\|\|\|\|\|: \|\| \|\|\|\|:@LB@ GGCAGUU UC UCCUU@LB@3'-UUU...AACCG AAAGU...ACA-5'@LB@ \| \|@LB@ 58 43@LB@@LB@@LB@Energy -5.02 kcal/mol@LB@Hybridization Energy -11.1502 kcal/mol@LB@Unfolding Energy - mRNA 3.39036 kcal/mol@LB@Unfolding Energy - sRNA 2.73756 kcal/mol@LB@@LB@Position - mRNA 179 -- 195@LB@Position - sRNA 44 -- 57@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 51 -- 57@LB@
36	0.007218	0.6129	b3938	metJ	-9.22	0.059290	181 -- 189	45 -- 53	transcriptional repressor S-adenosylmethionine-binding	_____________180_______190\n_____________\|_________\|\n5'-UCU...AUUGA_________UUAAG...UGU-3'\n______________CGAAGAGGA\n______________\|:\|\|\|\|\|\|\|\n______________GUUUCUCCU\n3'-UUU...GGGCA_________UAAAG...ACA-5'\n_____________\|_________\|\n_____________54________44\n	183 -- 189	45 -- 51	-12.478 kcal/mol	2.34125 kcal/mol	0.917553 kcal/mol	180 190@LB@ \| \|@LB@5'-UCU...AUUGA UUAAG...UGU-3'@LB@ CGAAGAGGA@LB@ \|:\|\|\|\|\|\|\|@LB@ GUUUCUCCU@LB@3'-UUU...GGGCA UAAAG...ACA-5'@LB@ \| \|@LB@ 54 44@LB@@LB@@LB@Energy -9.22 kcal/mol@LB@Hybridization Energy -12.478 kcal/mol@LB@Unfolding Energy - mRNA 2.34125 kcal/mol@LB@Unfolding Energy - sRNA 0.917553 kcal/mol@LB@@LB@Position - mRNA 181 -- 189@LB@Position - sRNA 45 -- 53@LB@Position Seed - mRNA 183 -- 189@LB@Position Seed - sRNA 45 -- 51@LB@
104	0.02144	0.6177	b2718	hycH	-8.07	0.100760	181 -- 190	46 -- 55	hydrogenase 3 maturation protein	_____________180________191\n_____________\|__________\|\n5'-CAG...AUGUU__________CGCGU...UGG-3'\n______________GUUGAAGAGG\n______________\|\|::\|\|\|\|\|\|\n______________CAGUUUCUCC\n3'-UUU...CCGGG__________UUAAA...ACA-5'\n_____________\|__________\|\n_____________56_________45\n	184 -- 190	46 -- 52	-11.8031 kcal/mol	2.8078 kcal/mol	0.924411 kcal/mol	180 191@LB@ \| \|@LB@5'-CAG...AUGUU CGCGU...UGG-3'@LB@ GUUGAAGAGG@LB@ \|\|::\|\|\|\|\|\|@LB@ CAGUUUCUCC@LB@3'-UUU...CCGGG UUAAA...ACA-5'@LB@ \| \|@LB@ 56 45@LB@@LB@@LB@Energy -8.07 kcal/mol@LB@Hybridization Energy -11.8031 kcal/mol@LB@Unfolding Energy - mRNA 2.8078 kcal/mol@LB@Unfolding Energy - sRNA 0.924411 kcal/mol@LB@@LB@Position - mRNA 181 -- 190@LB@Position - sRNA 46 -- 55@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 46 -- 52@LB@
143	0.02773	0.6177	b0409	secF	-7.84	0.111773	181 -- 195	43 -- 55	SecYEG protein translocase auxillary subunit	_____________180_____________196\n_____________\|_______________\|\n5'-CUU...AAGCU_____UCU_______GCGAU...UGU-3'\n______________GUCAA___GAGGAGU\n______________\|\|\|\|\|___\|\|\|\|\|:\|\n______________CAGUU___CUCCUUA\n3'-UUU...CCGGG_____U_________AAGUA...ACA-5'\n_____________\|_______________\|\n_____________56______________42\n	189 -- 195	43 -- 49	-13.7308 kcal/mol	4.94116 kcal/mol	0.946481 kcal/mol	180 196@LB@ \| \|@LB@5'-CUU...AAGCU UCU GCGAU...UGU-3'@LB@ GUCAA GAGGAGU@LB@ \|\|\|\|\| \|\|\|\|\|:\|@LB@ CAGUU CUCCUUA@LB@3'-UUU...CCGGG U AAGUA...ACA-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -7.84 kcal/mol@LB@Hybridization Energy -13.7308 kcal/mol@LB@Unfolding Energy - mRNA 4.94116 kcal/mol@LB@Unfolding Energy - sRNA 0.946481 kcal/mol@LB@@LB@Position - mRNA 181 -- 195@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 43 -- 49@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching