Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
167	0.03387	0.6177	b3980	tufB	-7.70	0.119070	183 -- 194	44 -- 55	translation elongation factor EF-Tu 2	b3339	_____________182__________195\n_____________\|____________\|\n5'-UCC...UCCGU___UU_______CAAUC...CUA-3'\n______________GUC__AGAGGGA\n______________\|\|\|__\|\|\|\|\|:\|\n______________CAG__UCUCCUU\n3'-UUU...CCGGG___UU_______AAAGU...ACA-5'\n_____________\|____________\|\n_____________56___________43\n	188 -- 194	44 -- 50	-14.3886 kcal/mol	5.74361 kcal/mol	0.941432 kcal/mol	182 195@LB@ \| \|@LB@5'-UCC...UCCGU UU CAAUC...CUA-3'@LB@ GUC AGAGGGA@LB@ \|\|\| \|\|\|\|\|:\|@LB@ CAG UCUCCUU@LB@3'-UUU...CCGGG UU AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -7.70 kcal/mol@LB@Hybridization Energy -14.3886 kcal/mol@LB@Unfolding Energy - mRNA 5.74361 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 183 -- 194@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 44 -- 50@LB@
168	0.03398	0.6177	b0321	yahG	-8.56	0.080369	183 -- 192	44 -- 53	DUF1116 family protein	b4572	_____________182________193\n_____________\|__________\|\n5'-AUC...AUCUG__________GAAGC...AAC-3'\n______________CAAAGGGGAG\n______________\|\|\|\|\|:\|\|\|:\n______________GUUUCUCCUU\n3'-UUU...GGGCA__________AAAGU...ACA-5'\n_____________\|__________\|\n_____________54_________43\n	186 -- 192	44 -- 50	-12.9704 kcal/mol	3.47901 kcal/mol	0.927997 kcal/mol	182 193@LB@ \| \|@LB@5'-AUC...AUCUG GAAGC...AAC-3'@LB@ CAAAGGGGAG@LB@ \|\|\|\|\|:\|\|\|:@LB@ GUUUCUCCUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -8.56 kcal/mol@LB@Hybridization Energy -12.9704 kcal/mol@LB@Unfolding Energy - mRNA 3.47901 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 183 -- 192@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 44 -- 50@LB@
9	0.001801	0.593	b1276	acnA	-10.22	0.037037	184 -- 198	44 -- 55	aconitate hydratase 1; aconitase A		_____________183_____________199\n_____________\|_______________\|\n5'-CUU...ACCCU____UUA________CUAUG...GCG-3'\n______________GUCA___AGGAGGAG\n______________\|\|\|\|___\|:\|\|\|\|\|:\n______________CAGU___UUCUCCUU\n3'-UUU...CCGGG_______________AAAGU...ACA-5'\n_____________\|_______________\|\n_____________56______________43\n	192 -- 198	44 -- 50	-13.3918 kcal/mol	2.22696 kcal/mol	0.941432 kcal/mol	183 199@LB@ \| \|@LB@5'-CUU...ACCCU UUA CUAUG...GCG-3'@LB@ GUCA AGGAGGAG@LB@ \|\|\|\| \|:\|\|\|\|\|:@LB@ CAGU UUCUCCUU@LB@3'-UUU...CCGGG AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -10.22 kcal/mol@LB@Hybridization Energy -13.3918 kcal/mol@LB@Unfolding Energy - mRNA 2.22696 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 184 -- 198@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 44 -- 50@LB@
20	0.00399	0.5992	b4175	hflC	-10.54	0.031960	184 -- 196	43 -- 56	HflB protease modulator specific for phage lambda cII repressor		_____________183____________197\n_____________\|______________\|\n5'-UUA...ACCAG______________AACGA...GUU-3'\n______________CGUCAGGG_GGAAU\n______________\|\|\|\|\|::\|_\|\|\|\|\|\n______________GCAGUUUC_CCUUA\n3'-UUU...ACCGG________U_____AAGUA...ACA-5'\n_____________\|______________\|\n_____________57_____________42\n	185 -- 191	49 -- 55	-15.8123 kcal/mol	4.32193 kcal/mol	0.953958 kcal/mol	183 197@LB@ \| \|@LB@5'-UUA...ACCAG AACGA...GUU-3'@LB@ CGUCAGGG GGAAU@LB@ \|\|\|\|\|::\| \|\|\|\|\|@LB@ GCAGUUUC CCUUA@LB@3'-UUU...ACCGG U AAGUA...ACA-5'@LB@ \| \|@LB@ 57 42@LB@@LB@@LB@Energy -10.54 kcal/mol@LB@Hybridization Energy -15.8123 kcal/mol@LB@Unfolding Energy - mRNA 4.32193 kcal/mol@LB@Unfolding Energy - sRNA 0.953958 kcal/mol@LB@@LB@Position - mRNA 184 -- 196@LB@Position - sRNA 43 -- 56@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 49 -- 55@LB@
29	0.005664	0.6127	b3831	udp	-8.88	0.069383	184 -- 196	42 -- 54	uridine phosphorylase		_____________183___________197\n_____________\|_____________\|\n5'-AUC...CCGAU___C_________GUAUA...AGA-3'\n______________UCA_AGAGGAGUU\n______________\|\|\|_\|\|\|\|\|\|:\|\|\n______________AGU_UCUCCUUAA\n3'-UUU...CGGGC___U_________AGUAA...ACA-5'\n_____________\|_____________\|\n_____________55____________41\n	190 -- 196	42 -- 48	-14.4874 kcal/mol	4.66075 kcal/mol	0.945538 kcal/mol	183 197@LB@ \| \|@LB@5'-AUC...CCGAU C GUAUA...AGA-3'@LB@ UCA AGAGGAGUU@LB@ \|\|\| \|\|\|\|\|\|:\|\|@LB@ AGU UCUCCUUAA@LB@3'-UUU...CGGGC U AGUAA...ACA-5'@LB@ \| \|@LB@ 55 41@LB@@LB@@LB@Energy -8.88 kcal/mol@LB@Hybridization Energy -14.4874 kcal/mol@LB@Unfolding Energy - mRNA 4.66075 kcal/mol@LB@Unfolding Energy - sRNA 0.945538 kcal/mol@LB@@LB@Position - mRNA 184 -- 196@LB@Position - sRNA 42 -- 54@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 42 -- 48@LB@
34	0.007003	0.6129	b4199	yjfY	-12.43	0.013073	184 -- 195	46 -- 57	YhcN family protein periplasmic		_____________183__________196\n_____________\|____________\|\n5'-AGG...GUCAG____________UAUCU...ACG-3'\n______________CCGUCGAGGAGG\n______________\|\|\|\|\|:\|:\|\|\|\|\n______________GGCAGUUUCUCC\n3'-UUU...AACCG____________UUAAA...ACA-5'\n_____________\|____________\|\n_____________58___________45\n	189 -- 195	46 -- 52	-19.9606 kcal/mol	4.79886 kcal/mol	2.7365 kcal/mol	183 196@LB@ \| \|@LB@5'-AGG...GUCAG UAUCU...ACG-3'@LB@ CCGUCGAGGAGG@LB@ \|\|\|\|\|:\|:\|\|\|\|@LB@ GGCAGUUUCUCC@LB@3'-UUU...AACCG UUAAA...ACA-5'@LB@ \| \|@LB@ 58 45@LB@@LB@@LB@Energy -12.43 kcal/mol@LB@Hybridization Energy -19.9606 kcal/mol@LB@Unfolding Energy - mRNA 4.79886 kcal/mol@LB@Unfolding Energy - sRNA 2.7365 kcal/mol@LB@@LB@Position - mRNA 184 -- 195@LB@Position - sRNA 46 -- 57@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 46 -- 52@LB@
43	0.008509	0.6129	b4322	uxuA	-11.91	0.016653	184 -- 195	44 -- 55	mannonate hydrolase		_____________183__________196\n_____________\|____________\|\n5'-CAU...ACUAU__G_________AAAUC...AUA-3'\n______________GU_AAAGAGGAA\n______________\|\|_\|\|\|\|\|\|\|\|\|\n______________CA_UUUCUCCUU\n3'-UUU...CCGGG__G_________AAAGU...ACA-5'\n_____________\|____________\|\n_____________56___________43\n	189 -- 195	44 -- 50	-15.5118 kcal/mol	2.65561 kcal/mol	0.941432 kcal/mol	183 196@LB@ \| \|@LB@5'-CAU...ACUAU G AAAUC...AUA-3'@LB@ GU AAAGAGGAA@LB@ \|\| \|\|\|\|\|\|\|\|\|@LB@ CA UUUCUCCUU@LB@3'-UUU...CCGGG G AAAGU...ACA-5'@LB@ \| \|@LB@ 56 43@LB@@LB@@LB@Energy -11.91 kcal/mol@LB@Hybridization Energy -15.5118 kcal/mol@LB@Unfolding Energy - mRNA 2.65561 kcal/mol@LB@Unfolding Energy - sRNA 0.941432 kcal/mol@LB@@LB@Position - mRNA 184 -- 195@LB@Position - sRNA 44 -- 55@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 44 -- 50@LB@
44	0.008636	0.6129	b3317	rplB	-9.78	0.045660	184 -- 194	43 -- 55	50S ribosomal subunit protein L2		_____________183___________195\n_____________\|_____________\|\n5'-GCA...AGUAA_____________AAUAC...UGC-3'\n______________GUC__GGAGGAGU\n______________\|\|\|__:\|\|\|\|\|:\|\n______________CAG__UCUCCUUA\n3'-UUU...CCGGG___UU________AAGUA...ACA-5'\n_____________\|_____________\|\n_____________56____________42\n	188 -- 194	43 -- 49	-13.3143 kcal/mol	2.58995 kcal/mol	0.946481 kcal/mol	183 195@LB@ \| \|@LB@5'-GCA...AGUAA AAUAC...UGC-3'@LB@ GUC GGAGGAGU@LB@ \|\|\| :\|\|\|\|\|:\|@LB@ CAG UCUCCUUA@LB@3'-UUU...CCGGG UU AAGUA...ACA-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -9.78 kcal/mol@LB@Hybridization Energy -13.3143 kcal/mol@LB@Unfolding Energy - mRNA 2.58995 kcal/mol@LB@Unfolding Energy - sRNA 0.946481 kcal/mol@LB@@LB@Position - mRNA 184 -- 194@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 43 -- 49@LB@
66	0.01414	0.6177	b0440	hupB	-10.30	0.035643	184 -- 193	44 -- 53	HU DNA-binding transcriptional regulator beta subunit		_____________183________194\n_____________\|__________\|\n5'-CGC...AAUUA__________GAGAA...CUG-3'\n______________UAAAGAGGAA\n______________:\|\|\|\|\|\|\|\|\|\n______________GUUUCUCCUU\n3'-UUU...GGGCA__________AAAGU...ACA-5'\n_____________\|__________\|\n_____________54_________43\n	187 -- 193	44 -- 50	-11.552 kcal/mol	0.319105 kcal/mol	0.927997 kcal/mol	183 194@LB@ \| \|@LB@5'-CGC...AAUUA GAGAA...CUG-3'@LB@ UAAAGAGGAA@LB@ :\|\|\|\|\|\|\|\|\|@LB@ GUUUCUCCUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -10.30 kcal/mol@LB@Hybridization Energy -11.552 kcal/mol@LB@Unfolding Energy - mRNA 0.319105 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 184 -- 193@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 44 -- 50@LB@
79	0.01695	0.6177	b0435	bolA	-9.02	0.064980	184 -- 202	38 -- 55	stationary-phase morphogene transcriptional repressor for mreB; also regulator for dacA dacC and ampC		_____________183_________________203\n_____________\|___________________\|\n5'-UGA...GCGUU___G______AU_______GAUGA...CCG-3'\n______________GUC_GAGGAG__AUUUCAU\n______________\|\|\|_:\|:\|\|\|__\|\|\|\|\|\|\|\n______________CAG_UUUCUC__UAAAGUA\n3'-UUU...CCGGG__________CU_______AAAAA...ACA-5'\n_____________\|___________________\|\n_____________56__________________37\n	196 -- 202	38 -- 44	-15.2358 kcal/mol	5.04311 kcal/mol	1.17036 kcal/mol	183 203@LB@ \| \|@LB@5'-UGA...GCGUU G AU GAUGA...CCG-3'@LB@ GUC GAGGAG AUUUCAU@LB@ \|\|\| :\|:\|\|\| \|\|\|\|\|\|\|@LB@ CAG UUUCUC UAAAGUA@LB@3'-UUU...CCGGG CU AAAAA...ACA-5'@LB@ \| \|@LB@ 56 37@LB@@LB@@LB@Energy -9.02 kcal/mol@LB@Hybridization Energy -15.2358 kcal/mol@LB@Unfolding Energy - mRNA 5.04311 kcal/mol@LB@Unfolding Energy - sRNA 1.17036 kcal/mol@LB@@LB@Position - mRNA 184 -- 202@LB@Position - sRNA 38 -- 55@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 38 -- 44@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching