Input and runtime details for job 1765463 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1765463 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:41:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:42:13 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 19:57:45 CET 2018
	Post-Processing Finished		@ Fri Feb 16 19:57:53 CET 2018
	Job Completed		@ Fri Feb 16 19:58:19 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1765463&toolName=CopraRNA ( 30 days expiry )

Description of the job

5 ChiX sequences

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
197	0.04129	0.6177	b1205	ychH	-8.72	0.074776	153 -- 162	43 -- 52	DUF2583 family putative inner membrane protein	_____________152________163\n_____________\|__________\|\n5'-CAA...AUAAG__________AUUCC...UAC-3'\n______________GAAGAGGGGU\n______________:\|\|\|\|\|\|::\|\n______________UUUCUCCUUA\n3'-UUU...GGCAG__________AAGUA...ACA-5'\n_____________\|__________\|\n_____________53_________42\n	156 -- 162	43 -- 49	-11.7568 kcal/mol	2.17132 kcal/mol	0.865937 kcal/mol	152 163@LB@ \| \|@LB@5'-CAA...AUAAG AUUCC...UAC-3'@LB@ GAAGAGGGGU@LB@ :\|\|\|\|\|\|::\|@LB@ UUUCUCCUUA@LB@3'-UUU...GGCAG AAGUA...ACA-5'@LB@ \| \|@LB@ 53 42@LB@@LB@@LB@Energy -8.72 kcal/mol@LB@Hybridization Energy -11.7568 kcal/mol@LB@Unfolding Energy - mRNA 2.17132 kcal/mol@LB@Unfolding Energy - sRNA 0.865937 kcal/mol@LB@@LB@Position - mRNA 153 -- 162@LB@Position - sRNA 43 -- 52@LB@Position Seed - mRNA 156 -- 162@LB@Position Seed - sRNA 43 -- 49@LB@
185	0.03847	0.6177	b0621	dcuC	-6.69	0.186516	155 -- 171	26 -- 48	anaerobic C4-dicarboxylate transport	_____________154_____________________172\n_____________\|_______________________\|\n5'-UGC...GCUUU_______________________UACUU...UCG-3'\n______________AGGAAUUUU______UUAUUAUU\n______________\|\|\|\|\|\|\|\|:______\|\|\|\|\|\|\|\|\n______________UCCUUAAAG______AAUAAUAA\n3'-UUU...GUUUC_________UAAAAA________UACGG...ACA-5'\n_____________\|_______________________\|\n_____________49______________________25\n	165 -- 171	26 -- 32	-10.8714 kcal/mol	3.1068 kcal/mol	1.07117 kcal/mol	154 172@LB@ \| \|@LB@5'-UGC...GCUUU UACUU...UCG-3'@LB@ AGGAAUUUU UUAUUAUU@LB@ \|\|\|\|\|\|\|\|: \|\|\|\|\|\|\|\|@LB@ UCCUUAAAG AAUAAUAA@LB@3'-UUU...GUUUC UAAAAA UACGG...ACA-5'@LB@ \| \|@LB@ 49 25@LB@@LB@@LB@Energy -6.69 kcal/mol@LB@Hybridization Energy -10.8714 kcal/mol@LB@Unfolding Energy - mRNA 3.1068 kcal/mol@LB@Unfolding Energy - sRNA 1.07117 kcal/mol@LB@@LB@Position - mRNA 155 -- 171@LB@Position - sRNA 26 -- 48@LB@Position Seed - mRNA 165 -- 171@LB@Position Seed - sRNA 26 -- 32@LB@
21	0.004054	0.5992	b2076	mdtC	-11.67	0.018717	158 -- 193	45 -- 78	multidrug efflux system subunit C	_____________157____________________________________194\n_____________\|______________________________________\|\n5'-CUC...GCAUU____A____AAGC____UU__________UG_______GGCGU...CGG-3'\n______________GUGG_CCAA____CGCU____GCCCGUCA__AAGAGGA\n______________:\|\|\|_\|\|\|\|____\|\|\|\|____\|\|\|\|\|\|\|\|__\|\|\|\|\|\|\|\n______________UACC_GGUU____GCGA____CGGGCAGU__UUCUCCU\n_____3'-UUUUUU_________AUA_____UAAC_________________UAAAG...ACA-5'\n_____________\|______________________________________\|\n_____________79_____________________________________44\n	187 -- 193	45 -- 51	-37.8051 kcal/mol	12.6053 kcal/mol	13.5316 kcal/mol	157 194@LB@ \| \|@LB@5'-CUC...GCAUU A AAGC UU UG GGCGU...CGG-3'@LB@ GUGG CCAA CGCU GCCCGUCA AAGAGGA@LB@ :\|\|\| \|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\|\|\|@LB@ UACC GGUU GCGA CGGGCAGU UUCUCCU@LB@ 3'-UUUUUU AUA UAAC UAAAG...ACA-5'@LB@ \| \|@LB@ 79 44@LB@@LB@@LB@Energy -11.67 kcal/mol@LB@Hybridization Energy -37.8051 kcal/mol@LB@Unfolding Energy - mRNA 12.6053 kcal/mol@LB@Unfolding Energy - sRNA 13.5316 kcal/mol@LB@@LB@Position - mRNA 158 -- 193@LB@Position - sRNA 45 -- 78@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 45 -- 51@LB@
135	0.02628	0.6177	b3547	yhjX	-9.70	0.047334	158 -- 184	28 -- 55	pyruvate-inducible inner membrane protein putative transporter	_____________157___________________________185\n_____________\|_____________________________\|\n5'-UAA...UGGUU_____C____AUU____C___________CUGCA...UUA-3'\n______________GUCGG_AGAG___UUUC___UUUUUAUUA\n______________\|\|\|::_\|\|\|\|___\|\|\|\|___\|\|\|\|\|\|\|\|\|\n______________CAGUU_UCUC___AAAG___AAAAAUAAU\n3'-UUU...CCGGG__________CUU____UAA_________AAUAC...ACA-5'\n_____________\|_____________________________\|\n_____________56____________________________27\n	178 -- 184	28 -- 34	-15.2509 kcal/mol	4.36169 kcal/mol	1.18814 kcal/mol	157 185@LB@ \| \|@LB@5'-UAA...UGGUU C AUU C CUGCA...UUA-3'@LB@ GUCGG AGAG UUUC UUUUUAUUA@LB@ \|\|\|:: \|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ CAGUU UCUC AAAG AAAAAUAAU@LB@3'-UUU...CCGGG CUU UAA AAUAC...ACA-5'@LB@ \| \|@LB@ 56 27@LB@@LB@@LB@Energy -9.70 kcal/mol@LB@Hybridization Energy -15.2509 kcal/mol@LB@Unfolding Energy - mRNA 4.36169 kcal/mol@LB@Unfolding Energy - sRNA 1.18814 kcal/mol@LB@@LB@Position - mRNA 158 -- 184@LB@Position - sRNA 28 -- 55@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 28 -- 34@LB@
8	0.001474	0.5928	b1048	opgG	-11.67	0.018726	159 -- 168	44 -- 53	OPG biosynthetic periplasmic beta-1 6 branching glycosyltransferase	_____________158________169\n_____________\|__________\|\n5'-ACA...GCACA__________AGUGC...AAU-3'\n______________CAAAGGGGGA\n______________\|\|\|\|\|:\|\|:\|\n______________GUUUCUCCUU\n3'-UUU...GGGCA__________AAAGU...ACA-5'\n_____________\|__________\|\n_____________54_________43\n	162 -- 168	44 -- 50	-12.9473 kcal/mol	0.352192 kcal/mol	0.927997 kcal/mol	158 169@LB@ \| \|@LB@5'-ACA...GCACA AGUGC...AAU-3'@LB@ CAAAGGGGGA@LB@ \|\|\|\|\|:\|\|:\|@LB@ GUUUCUCCUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -11.67 kcal/mol@LB@Hybridization Energy -12.9473 kcal/mol@LB@Unfolding Energy - mRNA 0.352192 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 159 -- 168@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 44 -- 50@LB@
97	0.01904	0.6177	b3102	yqjG	-9.04	0.064335	159 -- 168	44 -- 53	putative S-transferase	_____________158________169\n_____________\|__________\|\n5'-GCA...AAAAA__________GCUCC...GGC-3'\n______________CGAGGAGGAA\n______________\|:\|:\|\|\|\|\|\|\n______________GUUUCUCCUU\n3'-UUU...GGGCA__________AAAGU...ACA-5'\n_____________\|__________\|\n_____________54_________43\n	162 -- 168	44 -- 50	-13.5343 kcal/mol	3.56251 kcal/mol	0.927997 kcal/mol	158 169@LB@ \| \|@LB@5'-GCA...AAAAA GCUCC...GGC-3'@LB@ CGAGGAGGAA@LB@ \|:\|:\|\|\|\|\|\|@LB@ GUUUCUCCUU@LB@3'-UUU...GGGCA AAAGU...ACA-5'@LB@ \| \|@LB@ 54 43@LB@@LB@@LB@Energy -9.04 kcal/mol@LB@Hybridization Energy -13.5343 kcal/mol@LB@Unfolding Energy - mRNA 3.56251 kcal/mol@LB@Unfolding Energy - sRNA 0.927997 kcal/mol@LB@@LB@Position - mRNA 159 -- 168@LB@Position - sRNA 44 -- 53@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 44 -- 50@LB@
106	0.02162	0.6177	b1831	proQ	-7.28	0.143813	159 -- 202	38 -- 75	RNA chaperone putative ProP translation regulator	_____________158_____________________________________________203\n_____________\|_______________________________________________\|\n5'-UUA...UUCAU___UG______U________A____CGUUGU__UC___A________GGAAA...CGC-3'\n______________GCC_____CGC_UGUUGGCU_CGUC______AA__AGG_AAUUUCAU\n______________\|\|\|_____\|\|\|_\|:\|\|\|\|\|:_\|\|\|\|______\|\|__\|\|\|_\|\|\|\|\|\|\|\|\n______________CGG_____GCG_AUAACCGG_GCAG______UU__UCC_UUAAAGUA\n3'-UUU...UUUAC___UUAUA_________________________UC____________AAAAA...ACA-5'\n_____________\|_______________________________________________\|\n_____________76______________________________________________37\n	196 -- 202	38 -- 44	-31.8607 kcal/mol	10.974 kcal/mol	13.6034 kcal/mol	158 203@LB@ \| \|@LB@5'-UUA...UUCAU UG U A CGUUGU UC A GGAAA...CGC-3'@LB@ GCC CGC UGUUGGCU CGUC AA AGG AAUUUCAU@LB@ \|\|\| \|\|\| \|:\|\|\|\|\|: \|\|\|\| \|\| \|\|\| \|\|\|\|\|\|\|\|@LB@ CGG GCG AUAACCGG GCAG UU UCC UUAAAGUA@LB@3'-UUU...UUUAC UUAUA UC AAAAA...ACA-5'@LB@ \| \|@LB@ 76 37@LB@@LB@@LB@Energy -7.28 kcal/mol@LB@Hybridization Energy -31.8607 kcal/mol@LB@Unfolding Energy - mRNA 10.974 kcal/mol@LB@Unfolding Energy - sRNA 13.6034 kcal/mol@LB@@LB@Position - mRNA 159 -- 202@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 38 -- 44@LB@
13	0.003065	0.593	b4529	ydbJ	-10.82	0.027981	161 -- 194	44 -- 78	DUF333 family putative lipoprotein	_____________160___________________________________195\n_____________\|_____________________________________\|\n5'-GCA...UAUUU________________________AA__UC_______GAAAA...GUU-3'\n______________AUGG_CGGUGUCGU__UUGGCUUG__GA__AGAGGAA\n______________\|\|\|\|_\|::\|:\|\|\|:__\|\|\|\|\|::\|__:\|__\|\|\|\|\|\|\|\n______________UACC_GUUAUAGCG__AACCGGGC__UU__UCUCCUU\n_____3'-UUUUUU____G_________AU________AG___________AAAGU...ACA-5'\n_____________\|_____________________________________\|\n_____________79____________________________________43\n	188 -- 194	44 -- 50	-34.9495 kcal/mol	10.5981 kcal/mol	13.5333 kcal/mol	160 195@LB@ \| \|@LB@5'-GCA...UAUUU AA UC GAAAA...GUU-3'@LB@ AUGG CGGUGUCGU UUGGCUUG GA AGAGGAA@LB@ \|\|\|\| \|::\|:\|\|\|: \|\|\|\|\|::\| :\| \|\|\|\|\|\|\|@LB@ UACC GUUAUAGCG AACCGGGC UU UCUCCUU@LB@ 3'-UUUUUU G AU AG AAAGU...ACA-5'@LB@ \| \|@LB@ 79 43@LB@@LB@@LB@Energy -10.82 kcal/mol@LB@Hybridization Energy -34.9495 kcal/mol@LB@Unfolding Energy - mRNA 10.5981 kcal/mol@LB@Unfolding Energy - sRNA 13.5333 kcal/mol@LB@@LB@Position - mRNA 161 -- 194@LB@Position - sRNA 44 -- 78@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 44 -- 50@LB@
31	0.005902	0.6127	b1951	rcsA	-8.09	0.100100	163 -- 178	37 -- 51	transcriptional regulator of colanic acid capsular biosynthesis	_____________162______________179\n_____________\|________________\|\n5'-GUU...CAGGU__________A_____ACGCA...UUG-3'\n______________AAGGGGAAUU_UCGUU\n______________\|\|\|:\|\|\|\|\|\|_\|\|:\|\|\n______________UUCUCCUUAA_AGUAA\n3'-UUU...GCAGU________________AAAAA...ACA-5'\n_____________\|________________\|\n_____________52_______________36\n	166 -- 172	42 -- 48	-14.3412 kcal/mol	5.18535 kcal/mol	1.07049 kcal/mol	162 179@LB@ \| \|@LB@5'-GUU...CAGGU A ACGCA...UUG-3'@LB@ AAGGGGAAUU UCGUU@LB@ \|\|\|:\|\|\|\|\|\| \|\|:\|\|@LB@ UUCUCCUUAA AGUAA@LB@3'-UUU...GCAGU AAAAA...ACA-5'@LB@ \| \|@LB@ 52 36@LB@@LB@@LB@Energy -8.09 kcal/mol@LB@Hybridization Energy -14.3412 kcal/mol@LB@Unfolding Energy - mRNA 5.18535 kcal/mol@LB@Unfolding Energy - sRNA 1.07049 kcal/mol@LB@@LB@Position - mRNA 163 -- 178@LB@Position - sRNA 37 -- 51@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 42 -- 48@LB@
73	0.01516	0.6177	b0619	citA	-15.44	0.003129	164 -- 175	46 -- 57	sensory histidine kinase in two-component regulatory system with CitB	_____________163__________176\n_____________\|____________\|\n5'-CAU...UACAU____________CUCGG...UCA-3'\n______________CCGUCAAAGAGG\n______________\|\|\|\|\|\|\|\|\|\|\|\|\n______________GGCAGUUUCUCC\n3'-UUU...AACCG____________UUAAA...ACA-5'\n_____________\|____________\|\n_____________58___________45\n	169 -- 175	46 -- 52	-20.3901 kcal/mol	2.21322 kcal/mol	2.7365 kcal/mol	163 176@LB@ \| \|@LB@5'-CAU...UACAU CUCGG...UCA-3'@LB@ CCGUCAAAGAGG@LB@ \|\|\|\|\|\|\|\|\|\|\|\|@LB@ GGCAGUUUCUCC@LB@3'-UUU...AACCG UUAAA...ACA-5'@LB@ \| \|@LB@ 58 45@LB@@LB@@LB@Energy -15.44 kcal/mol@LB@Hybridization Energy -20.3901 kcal/mol@LB@Unfolding Energy - mRNA 2.21322 kcal/mol@LB@Unfolding Energy - sRNA 2.7365 kcal/mol@LB@@LB@Position - mRNA 164 -- 175@LB@Position - sRNA 46 -- 57@LB@Position Seed - mRNA 169 -- 175@LB@Position Seed - sRNA 46 -- 52@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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