Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
184	0.02128	0.3833	b2831	mutH	-12.07	0.107608	142 -- 230	114 -- 189	methyl-directed mismatch repair protein	_____________141______________231\n_____________\|________________\|\n5'-UUC...CUGCG________________AUUGC...CGG-3'\n______________AAUAUUCGG_CACAUA\n______________\|\|\|\|:\|\|:\|_\|\|\|\|:\|\n______________UUAUGAGUC_GUGUGU\n3'-UUU...UGUGG_________A______ACUAC...UAG-5'\n_____________\|________________\|\n_____________190______________113\n	154 -- 160	174 -- 180	-15.1193 kcal/mol	2.199 kcal/mol	0.596263 kcal/mol	141 231@LB@ \| \|@LB@5'-UUC...CUGCG AUUGC...CGG-3'@LB@ AAUAUUCGG CACAUA@LB@ \|\|\|\|:\|\|:\| \|\|\|\|:\|@LB@ UUAUGAGUC GUGUGU@LB@3'-UUU...UGUGG A ACUAC...UAG-5'@LB@ \| \|@LB@ 190 113@LB@@LB@@LB@Energy -12.07 kcal/mol@LB@Hybridization Energy -15.1193 kcal/mol@LB@Unfolding Energy - mRNA 2.199 kcal/mol@LB@Unfolding Energy - sRNA 0.596263 kcal/mol@LB@@LB@Position - mRNA 142 -- 230@LB@Position - sRNA 114 -- 189@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 174 -- 180@LB@
117	0.01283	0.3575	b3096	mzrA	-10.41	0.230387	15 -- 55	168 -- 208	modulator of EnvZ/OmpR regulon	_____________14________________________________________________________56\n_____________\|_________________________________________________________\|\n_____5'-UCAACU____G____________________________GAUCCUGA___CU__G________GCUCG...AGC-3'\n______________GGAU_AGCGG______UCUGCU_GUGGGU_AUU________CAA__CU_GGUUACAU\n______________::\|:_\|\|\|:\|______\|\|\|\|\|\|_\|:\|\|\|\|_\|\|\|________\|\|\|__\|\|_:\|\|:\|\|\|:\n______________UUUG_UCGUC______AGACGA_CGCCCA_UAA________GUU__GA_UCAGUGUG\n______3'-UUUUU____G_____CAUAUU______C______C___AAUGUG_____AU__G________UACUA...UAG-5'\n_____________\|_________________________________________________________\|\n_____________209_______________________________________________________167\n	49 -- 55	168 -- 174	-40.4023 kcal/mol	11.8856 kcal/mol	18.3074 kcal/mol	14 56@LB@ \| \|@LB@ 5'-UCAACU G GAUCCUGA CU G GCUCG...AGC-3'@LB@ GGAU AGCGG UCUGCU GUGGGU AUU CAA CU GGUUACAU@LB@ ::\|: \|\|\|:\| \|\|\|\|\|\| \|:\|\|\|\| \|\|\| \|\|\| \|\| :\|\|:\|\|\|:@LB@ UUUG UCGUC AGACGA CGCCCA UAA GUU GA UCAGUGUG@LB@ 3'-UUUUU G CAUAUU C C AAUGUG AU G UACUA...UAG-5'@LB@ \| \|@LB@ 209 167@LB@@LB@@LB@Energy -10.41 kcal/mol@LB@Hybridization Energy -40.4023 kcal/mol@LB@Unfolding Energy - mRNA 11.8856 kcal/mol@LB@Unfolding Energy - sRNA 18.3074 kcal/mol@LB@@LB@Position - mRNA 15 -- 55@LB@Position - sRNA 168 -- 208@LB@Position Seed - mRNA 49 -- 55@LB@Position Seed - sRNA 168 -- 174@LB@
123	0.01344	0.3575	b2615	nadK	-10.03	0.271821	99 -- 122	57 -- 77	NAD kinase	_____________98____________123\n_____________\|_____________\|\n5'-CUC...CCCAC________AC___ACUGA...AAG-3'\n______________GGCACCCC__UGC\n______________\|\|\|\|\|\|\|\|__\|\|\|\n______________CCGUGGGG__ACG\n3'-UUU...GUACC________GA___AAGUAG-5'\n_____________\|_____________\|\n_____________78____________56\n	245 -- 251	12 -- 18	-21.986 kcal/mol	5.26387 kcal/mol	8.16826 kcal/mol	98 123@LB@ \| \|@LB@5'-CUC...CCCAC AC ACUGA...AAG-3'@LB@ GGCACCCC UGC@LB@ \|\|\|\|\|\|\|\| \|\|\|@LB@ CCGUGGGG ACG@LB@3'-UUU...GUACC GA AAGUAG-5'@LB@ \| \|@LB@ 78 56@LB@@LB@@LB@Energy -10.03 kcal/mol@LB@Hybridization Energy -21.986 kcal/mol@LB@Unfolding Energy - mRNA 5.26387 kcal/mol@LB@Unfolding Energy - sRNA 8.16826 kcal/mol@LB@@LB@Position - mRNA 99 -- 122@LB@Position - sRNA 57 -- 77@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 12 -- 18@LB@
111	0.0122	0.3575	b1813	nudL	-9.44	0.347588	99 -- 131	174 -- 206	putative CoA pyrophosphohydrolase weak 3-phosphohydroxypyruvate phosphatase	_____________98________________________________132\n_____________\|_________________________________\|\n5'-CCU...UCUGC_____G_____UG__A__GU________GC___GAAGA...CUG-3'\n______________UCUGC_GGCGG__GA_UU__CGCCGAUA__CAG\n______________\|\|\|\|\|_\|\|\|\|\|__\|\|_\|\|__\|:\|\|:\|\|\|__\|\|\|\n______________AGACG_CCGCC__CU_AA__GUGGUUAU__GUC\n3'-UUU...AUAUU_____A_____CA__A__AU________GA___AGUGU...UAG-5'\n_____________\|_________________________________\|\n_____________207_______________________________173\n	120 -- 126	179 -- 185	-36.2385 kcal/mol	15.3331 kcal/mol	14.8345 kcal/mol	98 132@LB@ \| \|@LB@5'-CCU...UCUGC G UG A GU GC GAAGA...CUG-3'@LB@ UCUGC GGCGG GA UU CGCCGAUA CAG@LB@ \|\|\|\|\| \|\|\|\|\| \|\| \|\| \|:\|\|:\|\|\| \|\|\|@LB@ AGACG CCGCC CU AA GUGGUUAU GUC@LB@3'-UUU...AUAUU A CA A AU GA AGUGU...UAG-5'@LB@ \| \|@LB@ 207 173@LB@@LB@@LB@Energy -9.44 kcal/mol@LB@Hybridization Energy -36.2385 kcal/mol@LB@Unfolding Energy - mRNA 15.3331 kcal/mol@LB@Unfolding Energy - sRNA 14.8345 kcal/mol@LB@@LB@Position - mRNA 99 -- 131@LB@Position - sRNA 174 -- 206@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 179 -- 185@LB@
185	0.02128	0.3833	b2276	nuoN	-8.72	0.461673	62 -- 81	196 -- 211	NADH:ubiquinone oxidoreductase membrane subunit N	_____________61______________82\n_____________\|_______________\|\n5'-GUA...UAUGA__________U____GUGCU...AUC-3'\n______________UC_CUGUUGC_GGUG\n______________\|\|_\|\|\|\|:\|\|_\|\|\|:\n______________AG_GACAGCG_CCAU\n3'-UUU...GUUGA__C____________UUUAU...UAG-5'\n_____________\|_______________\|\n_____________212_____________195\n	69 -- 75	52 -- 58	-15.8669 kcal/mol	5.2118 kcal/mol	5.88061 kcal/mol	61 82@LB@ \| \|@LB@5'-GUA...UAUGA U GUGCU...AUC-3'@LB@ UC CUGUUGC GGUG@LB@ \|\| \|\|\|\|:\|\| \|\|\|:@LB@ AG GACAGCG CCAU@LB@3'-UUU...GUUGA C UUUAU...UAG-5'@LB@ \| \|@LB@ 212 195@LB@@LB@@LB@Energy -8.72 kcal/mol@LB@Hybridization Energy -15.8669 kcal/mol@LB@Unfolding Energy - mRNA 5.2118 kcal/mol@LB@Unfolding Energy - sRNA 5.88061 kcal/mol@LB@@LB@Position - mRNA 62 -- 81@LB@Position - sRNA 196 -- 211@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 52 -- 58@LB@
11	0.0006963	0.1842	b1739	osmE	-13.14	0.065080	250 -- 260	194 -- 204	osmotically-inducible lipoprotein	_____________249_________261\n_____________\|___________\|\n5'-GAA...AACCA___________UGUAC...AGC-3'\n______________UGCUGGCGGGU\n______________\|\|\|\|\|\|\|\|\|\|\|\n______________ACGACCGCCCA\n3'-UUU...AUUAG___________CUAAA...UAG-5'\n_____________\|___________\|\n_____________205_________193\n	254 -- 260	194 -- 200	-22.742 kcal/mol	5.67651 kcal/mol	8.00138 kcal/mol	249 261@LB@ \| \|@LB@5'-GAA...AACCA UGUAC...AGC-3'@LB@ UGCUGGCGGGU@LB@ \|\|\|\|\|\|\|\|\|\|\|@LB@ ACGACCGCCCA@LB@3'-UUU...AUUAG CUAAA...UAG-5'@LB@ \| \|@LB@ 205 193@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -22.742 kcal/mol@LB@Unfolding Energy - mRNA 5.67651 kcal/mol@LB@Unfolding Energy - sRNA 8.00138 kcal/mol@LB@@LB@Position - mRNA 250 -- 260@LB@Position - sRNA 194 -- 204@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 194 -- 200@LB@
92	0.01029	0.3575	b3360	pabA	-10.28	0.244097	93 -- 100	16 -- 23	aminodeoxychorismate synthase subunit II	_____________92__________101\n_____________\|___________\|\n5'-GCG...ACCUC___________UUGUG...CGU-3'\n______________UACCAGUACUU\n______________:\|\|\|\|:\|\|\|\|:\n______________GUGGUUAUGAG\n3'-UUU...AAAAU___________UCAGU...UAG-5'\n_____________\|___________\|\n_____________24__________15\n	250 -- 256	176 -- 182	-13.2399 kcal/mol	3.06354 kcal/mol	3.38772 kcal/mol	92 101@LB@ \| \|@LB@5'-GCG...ACCUC UUGUG...CGU-3'@LB@ UACCAGUACUU@LB@ :\|\|\|\|:\|\|\|\|:@LB@ GUGGUUAUGAG@LB@3'-UUU...AAAAU UCAGU...UAG-5'@LB@ \| \|@LB@ 24 15@LB@@LB@@LB@Energy -10.28 kcal/mol@LB@Hybridization Energy -13.2399 kcal/mol@LB@Unfolding Energy - mRNA 3.06354 kcal/mol@LB@Unfolding Energy - sRNA 3.38772 kcal/mol@LB@@LB@Position - mRNA 93 -- 100@LB@Position - sRNA 16 -- 23@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 176 -- 182@LB@
109	0.01201	0.3575	b1096	pabC	-8.08	0.578936	115 -- 139	144 -- 163	4-amino-4-deoxychorismate lyase component of para-aminobenzoate synthase multienzyme complex	_____________114____________140\n_____________\|______________\|\n5'-GUU...GUGUA_______CG_____GGUGG...ACG-3'\n______________ACUCCUU__GCUUC\n______________\|\|:\|\|:\|__\|\|\|\|\|\n______________UGGGGGA__CGAAG\n3'-UUU...CCCCG_______A______UAG-5'\n_____________\|______________\|\n_____________164____________143\n	29 -- 35	10 -- 16	-15.037 kcal/mol	0.971533 kcal/mol	6.96912 kcal/mol	114 140@LB@ \| \|@LB@5'-GUU...GUGUA CG GGUGG...ACG-3'@LB@ ACUCCUU GCUUC@LB@ \|\|:\|\|:\| \|\|\|\|\|@LB@ UGGGGGA CGAAG@LB@3'-UUU...CCCCG A UAG-5'@LB@ \| \|@LB@ 164 143@LB@@LB@@LB@Energy -8.08 kcal/mol@LB@Hybridization Energy -15.037 kcal/mol@LB@Unfolding Energy - mRNA 0.971533 kcal/mol@LB@Unfolding Energy - sRNA 6.96912 kcal/mol@LB@@LB@Position - mRNA 115 -- 139@LB@Position - sRNA 144 -- 163@LB@Position Seed - mRNA 29 -- 35@LB@Position Seed - sRNA 10 -- 16@LB@
25	0.002009	0.2633	b0134	panB	-9.88	0.290153	57 -- 67	176 -- 186	3-methyl-2-oxobutanoate hydroxymethyltransferase	_____________56__________68\n_____________\|___________\|\n5'-CAG...AAAGC___________UCCAG...UUG-3'\n______________CACCAGUACUU\n______________\|\|\|\|\|:\|\|\|\|:\n______________GUGGUUAUGAG\n3'-UUU...AAAAU___________UCAGU...UAG-5'\n_____________\|___________\|\n_____________187_________175\n	61 -- 67	176 -- 182	-15.2136 kcal/mol	0.973349 kcal/mol	3.38772 kcal/mol	56 68@LB@ \| \|@LB@5'-CAG...AAAGC UCCAG...UUG-3'@LB@ CACCAGUACUU@LB@ \|\|\|\|\|:\|\|\|\|:@LB@ GUGGUUAUGAG@LB@3'-UUU...AAAAU UCAGU...UAG-5'@LB@ \| \|@LB@ 187 175@LB@@LB@@LB@Energy -9.88 kcal/mol@LB@Hybridization Energy -15.2136 kcal/mol@LB@Unfolding Energy - mRNA 0.973349 kcal/mol@LB@Unfolding Energy - sRNA 3.38772 kcal/mol@LB@@LB@Position - mRNA 57 -- 67@LB@Position - sRNA 176 -- 186@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 176 -- 182@LB@
141	0.01524	0.3575	b0133	panC	-11.57	0.136229	4 -- 24	142 -- 162	pantothenate synthetase	_____________3_________25\n_____________\|_________\|\n5'-GGC...ACCGG_________UCCUA...UGC-3'\n______________CGGUCACAU\n______________\|:\|\|\|\|\|\|:\n______________GUCAGUGUG\n3'-UUU...UAUGA_________UACUA...UAG-5'\n_____________\|_________\|\n_____________163_______141\n	65 -- 71	168 -- 174	-12.1402 kcal/mol	2.67616 kcal/mol	0.276974 kcal/mol	3 25@LB@ \| \|@LB@5'-GGC...ACCGG UCCUA...UGC-3'@LB@ CGGUCACAU@LB@ \|:\|\|\|\|\|\|:@LB@ GUCAGUGUG@LB@3'-UUU...UAUGA UACUA...UAG-5'@LB@ \| \|@LB@ 163 141@LB@@LB@@LB@Energy -11.57 kcal/mol@LB@Hybridization Energy -12.1402 kcal/mol@LB@Unfolding Energy - mRNA 2.67616 kcal/mol@LB@Unfolding Energy - sRNA 0.276974 kcal/mol@LB@@LB@Position - mRNA 4 -- 24@LB@Position - sRNA 142 -- 162@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 168 -- 174@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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