Input and runtime details for job 6860187 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6860187 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:33 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:29:58 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 01:36:19 CET 2018
	Post-Processing Finished		@ Sat Feb 17 01:36:26 CET 2018
	Job Completed		@ Sat Feb 17 01:36:50 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6860187&toolName=CopraRNA ( 30 days expiry )

Description of the job

SgrS

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
61	0.006025	0.3261	b4330	yjiH	-10.68	0.204041	214 -- 227	80 -- 92	nucleoside recognition pore and gate family putative inner membrane transporter	_____________213______228\n_____________\|________\|\n5'-ACU...ACAAG________GUAGC...CCG-3'\n______________GGGAUGCU\n______________\|\|\|\|:\|\|\|\n______________CCCUGCGA\n3'-UUU...CAACA________AUUGG...UAG-5'\n_____________\|________\|\n_____________93_______79\n	215 -- 221	85 -- 91	-14.2816 kcal/mol	2.08889 kcal/mol	5.76908 kcal/mol	213 228@LB@ \| \|@LB@5'-ACU...ACAAG GUAGC...CCG-3'@LB@ GGGAUGCU@LB@ \|\|\|\|:\|\|\|@LB@ CCCUGCGA@LB@3'-UUU...CAACA AUUGG...UAG-5'@LB@ \| \|@LB@ 93 79@LB@@LB@@LB@Energy -10.68 kcal/mol@LB@Hybridization Energy -14.2816 kcal/mol@LB@Unfolding Energy - mRNA 2.08889 kcal/mol@LB@Unfolding Energy - sRNA 5.76908 kcal/mol@LB@@LB@Position - mRNA 214 -- 227@LB@Position - sRNA 80 -- 92@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 85 -- 91@LB@
156	0.01739	0.3749	b4332	yjiJ	-7.83	0.625728	95 -- 115	80 -- 101	DUF1228 family putative inner membrane MFS superfamily transporter	_____________94_______116\n_____________\|________\|\n5'-AAC...GGUUU________ACAUC...UUU-3'\n______________GUCGCAUA\n______________\|\|\|:\|\|:\|\n______________CAGUGUGU\n3'-UUU...UGAGU________ACUAC...UAG-5'\n_____________\|________\|\n_____________102______79\n	23 -- 29	167 -- 173	-9.78459 kcal/mol	1.62979 kcal/mol	0.409692 kcal/mol	94 116@LB@ \| \|@LB@5'-AAC...GGUUU ACAUC...UUU-3'@LB@ GUCGCAUA@LB@ \|\|\|:\|\|:\|@LB@ CAGUGUGU@LB@3'-UUU...UGAGU ACUAC...UAG-5'@LB@ \| \|@LB@ 102 79@LB@@LB@@LB@Energy -7.83 kcal/mol@LB@Hybridization Energy -9.78459 kcal/mol@LB@Unfolding Energy - mRNA 1.62979 kcal/mol@LB@Unfolding Energy - sRNA 0.409692 kcal/mol@LB@@LB@Position - mRNA 95 -- 115@LB@Position - sRNA 80 -- 101@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 167 -- 173@LB@
192	0.02246	0.3833	b4340	yjiR	-11.68	0.128907	36 -- 62	126 -- 153	putative DNA-binding transcriptional regulator/putative aminotransferase	_____________35_______63\n_____________\|________\|\n5'-AGC...CAGAG________AUCCA...GUC-3'\n______________GCUUCAUC\n______________\|\|\|\|\|\|\|\|\n______________CGAAGUAG\n3'-UUU...GGGAA________-5'\n_____________\|_______\|\n_____________154_____126\n	50 -- 56	1 -- 7	-12.7164 kcal/mol	1.73081 kcal/mol	0.703102 kcal/mol	35 63@LB@ \| \|@LB@5'-AGC...CAGAG AUCCA...GUC-3'@LB@ GCUUCAUC@LB@ \|\|\|\|\|\|\|\|@LB@ CGAAGUAG@LB@3'-UUU...GGGAA -5'@LB@ \| \|@LB@ 154 126@LB@@LB@@LB@Energy -11.68 kcal/mol@LB@Hybridization Energy -12.7164 kcal/mol@LB@Unfolding Energy - mRNA 1.73081 kcal/mol@LB@Unfolding Energy - sRNA 0.703102 kcal/mol@LB@@LB@Position - mRNA 36 -- 62@LB@Position - sRNA 126 -- 153@LB@Position Seed - mRNA 50 -- 56@LB@Position Seed - sRNA 1 -- 7@LB@
168	0.01937	0.3812	b4354	yjiY	-11.43	0.145336	99 -- 125	132 -- 159	putative transporter	_____________98__________________________________________________126\n_____________\|___________________________________________________\|\n5'-CUU...UACCU_____UACG_______AACUCU_____CA______A_______A_______AUUUA...ACA-3'\n______________UACUU____CGCGCGU______GGCAA__UC_ACU__CAGGAU_GCGGUCA\n______________\|\|\|\|:____\|:\|:\|:\|______\|\|\|\|\|__\|\|_\|\|\|__\|\|\|:\|\|_\|\|\|\|\|\|\|\n______________AUGAG____GUGUGUA______CCGUU__AG_UGA__GUCUUA_CGCCAGU\n3'-UUU...UGGUU_____UCA________CUACGU_____C___U___AA______A_______AGGGU...UAG-5'\n_____________\|___________________________________________________\|\n_____________160_________________________________________________131\n	119 -- 125	132 -- 138	-39.8751 kcal/mol	13.2469 kcal/mol	12.8104 kcal/mol	98 126@LB@ \| \|@LB@5'-CUU...UACCU UACG AACUCU CA A A AUUUA...ACA-3'@LB@ UACUU CGCGCGU GGCAA UC ACU CAGGAU GCGGUCA@LB@ \|\|\|\|: \|:\|:\|:\| \|\|\|\|\| \|\| \|\|\| \|\|\|:\|\| \|\|\|\|\|\|\|@LB@ AUGAG GUGUGUA CCGUU AG UGA GUCUUA CGCCAGU@LB@3'-UUU...UGGUU UCA CUACGU C U AA A AGGGU...UAG-5'@LB@ \| \|@LB@ 160 131@LB@@LB@@LB@Energy -11.43 kcal/mol@LB@Hybridization Energy -39.8751 kcal/mol@LB@Unfolding Energy - mRNA 13.2469 kcal/mol@LB@Unfolding Energy - sRNA 12.8104 kcal/mol@LB@@LB@Position - mRNA 99 -- 125@LB@Position - sRNA 132 -- 159@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 132 -- 138@LB@
146	0.01559	0.358	b4394	yjjX	-8.60	0.483218	101 -- 114	123 -- 137	non-canonical purine NTP phosphatase ITPase/XTPase	_____________100__________________________________115\n_____________\|____________________________________\|\n5'-UGC...AAUCC______A______________UC________UU___CGAGA...CAU-3'\n______________CGCUAA_AUUCAG__GC_CAU__UGCAGGCA__UCA\n______________\|:\|:\|\|_\|:\|\|\|\|__:\|_\|\|\|__\|\|\|\|\|\|\|\|__\|\|\|\n______________GUGGUU_UGAGUC__UG_GUA__ACGUCCGU__AGU\n3'-UUU...AAAAU______A______AG__U___CU________UC___UGAAA...UAG-5'\n_____________\|____________________________________\|\n_____________138__________________________________122\n	254 -- 260	156 -- 162	-32.2061 kcal/mol	8.98762 kcal/mol	12.5635 kcal/mol	100 115@LB@ \| \|@LB@5'-UGC...AAUCC A UC UU CGAGA...CAU-3'@LB@ CGCUAA AUUCAG GC CAU UGCAGGCA UCA@LB@ \|:\|:\|\| \|:\|\|\|\| :\| \|\|\| \|\|\|\|\|\|\|\| \|\|\|@LB@ GUGGUU UGAGUC UG GUA ACGUCCGU AGU@LB@3'-UUU...AAAAU A AG U CU UC UGAAA...UAG-5'@LB@ \| \|@LB@ 138 122@LB@@LB@@LB@Energy -8.60 kcal/mol@LB@Hybridization Energy -32.2061 kcal/mol@LB@Unfolding Energy - mRNA 8.98762 kcal/mol@LB@Unfolding Energy - sRNA 12.5635 kcal/mol@LB@@LB@Position - mRNA 101 -- 114@LB@Position - sRNA 123 -- 137@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 156 -- 162@LB@
126	0.01383	0.3575	b1843	yobB	-8.48	0.503741	101 -- 118	168 -- 185	C-N hydrolase family protein	_____________100____________119\n_____________\|______________\|\n5'-CCU...AACUU____AG________UUUCU...CCA-3'\n______________AUAC__AGUUACAC\n______________\|\|\|\|__\|\|\|:\|\|\|\|\n______________UAUG__UCAGUGUG\n3'-UUU...GUGGU____AG________UACUA...UAG-5'\n_____________\|______________\|\n_____________186____________167\n	112 -- 118	168 -- 174	-13.9608 kcal/mol	3.09724 kcal/mol	0.286881 kcal/mol	100 119@LB@ \| \|@LB@5'-CCU...AACUU AG UUUCU...CCA-3'@LB@ AUAC AGUUACAC@LB@ \|\|\|\| \|\|\|:\|\|\|\|@LB@ UAUG UCAGUGUG@LB@3'-UUU...GUGGU AG UACUA...UAG-5'@LB@ \| \|@LB@ 186 167@LB@@LB@@LB@Energy -8.48 kcal/mol@LB@Hybridization Energy -13.9608 kcal/mol@LB@Unfolding Energy - mRNA 3.09724 kcal/mol@LB@Unfolding Energy - sRNA 0.286881 kcal/mol@LB@@LB@Position - mRNA 101 -- 118@LB@Position - sRNA 168 -- 185@LB@Position Seed - mRNA 112 -- 118@LB@Position Seed - sRNA 168 -- 174@LB@
96	0.01055	0.3575	b1953	yodD	-11.39	0.147949	264 -- 273	196 -- 205	uncharacterized protein	_____________263_____274\n_____________\|_______\|\n5'-CCA...UUCAC_______GUUUU...AAG-3'\n______________CUUCAUC\n______________\|\|\|\|\|\|\|\n______________GAAGUAG\n3'-UUU...GGAAC_______-5'\n_____________\|______\|\n_____________206____196\n	33 -- 39	1 -- 7	-8.82286 kcal/mol	0.690587 kcal/mol	0.701705 kcal/mol	263 274@LB@ \| \|@LB@5'-CCA...UUCAC GUUUU...AAG-3'@LB@ CUUCAUC@LB@ \|\|\|\|\|\|\|@LB@ GAAGUAG@LB@3'-UUU...GGAAC -5'@LB@ \| \|@LB@ 206 196@LB@@LB@@LB@Energy -11.39 kcal/mol@LB@Hybridization Energy -8.82286 kcal/mol@LB@Unfolding Energy - mRNA 0.690587 kcal/mol@LB@Unfolding Energy - sRNA 0.701705 kcal/mol@LB@@LB@Position - mRNA 264 -- 273@LB@Position - sRNA 196 -- 205@LB@Position Seed - mRNA 33 -- 39@LB@Position Seed - sRNA 1 -- 7@LB@
46	0.004081	0.2985	b2466	ypfG	-10.37	0.233995	256 -- 280	68 -- 95	DUF1176 family protein	_____________255_____281\n_____________\|_______\|\n5'-AAA...CAAAC_______UUAAU...UCA-3'\n______________GUCACAU\n______________\|\|\|\|\|\|:\n______________CAGUGUG\n3'-UUU...UGAGU_______UACUA...UAG-5'\n_____________\|_______\|\n_____________96______67\n	57 -- 63	168 -- 174	-9.42181 kcal/mol	2.13246 kcal/mol	0.260649 kcal/mol	255 281@LB@ \| \|@LB@5'-AAA...CAAAC UUAAU...UCA-3'@LB@ GUCACAU@LB@ \|\|\|\|\|\|:@LB@ CAGUGUG@LB@3'-UUU...UGAGU UACUA...UAG-5'@LB@ \| \|@LB@ 96 67@LB@@LB@@LB@Energy -10.37 kcal/mol@LB@Hybridization Energy -9.42181 kcal/mol@LB@Unfolding Energy - mRNA 2.13246 kcal/mol@LB@Unfolding Energy - sRNA 0.260649 kcal/mol@LB@@LB@Position - mRNA 256 -- 280@LB@Position - sRNA 68 -- 95@LB@Position Seed - mRNA 57 -- 63@LB@Position Seed - sRNA 168 -- 174@LB@
36	0.002935	0.2744	b3152	yraR	-13.11	0.066158	241 -- 255	123 -- 138	putative nucleoside-diphosphate-sugar epimerase	_____________240______________256\n_____________\|________________\|\n5'-ACU...GGUGG________________GGAUG...CGA-3'\n______________GCGGUCA_CCUGCUGC\n______________\|\|\|\|\|\|\|_\|\|::\|\|\|\|\n______________CGCCAGU_GGGUGACG\n3'-UUU...CUUAA_______A________UAGUC...UAG-5'\n_____________\|________________\|\n_____________139______________122\n	241 -- 247	132 -- 138	-26.7154 kcal/mol	6.55898 kcal/mol	13.7588 kcal/mol	240 256@LB@ \| \|@LB@5'-ACU...GGUGG GGAUG...CGA-3'@LB@ GCGGUCA CCUGCUGC@LB@ \|\|\|\|\|\|\| \|\|::\|\|\|\|@LB@ CGCCAGU GGGUGACG@LB@3'-UUU...CUUAA A UAGUC...UAG-5'@LB@ \| \|@LB@ 139 122@LB@@LB@@LB@Energy -13.11 kcal/mol@LB@Hybridization Energy -26.7154 kcal/mol@LB@Unfolding Energy - mRNA 6.55898 kcal/mol@LB@Unfolding Energy - sRNA 13.7588 kcal/mol@LB@@LB@Position - mRNA 241 -- 255@LB@Position - sRNA 123 -- 138@LB@Position Seed - mRNA 241 -- 247@LB@Position Seed - sRNA 132 -- 138@LB@
43	0.003721	0.2912	b3232	zapE	-10.85	0.189153	236 -- 256	70 -- 90	divisome ATPase	_____________235___________257\n_____________\|_____________\|\n5'-AGG...AACGG____UU_______AACCG...AAA-3'\n______________CCUU__UUUCGUC\n______________\|\|\|\|__:\|\|\|:\|\|\n______________GGAA__GAAGUAG\n3'-UUU...GUGGG____C________-5'\n_____________\|____________\|\n_____________91___________70\n	146 -- 152	1 -- 7	-9.27362 kcal/mol	0.691484 kcal/mol	1.04836 kcal/mol	235 257@LB@ \| \|@LB@5'-AGG...AACGG UU AACCG...AAA-3'@LB@ CCUU UUUCGUC@LB@ \|\|\|\| :\|\|\|:\|\|@LB@ GGAA GAAGUAG@LB@3'-UUU...GUGGG C -5'@LB@ \| \|@LB@ 91 70@LB@@LB@@LB@Energy -10.85 kcal/mol@LB@Hybridization Energy -9.27362 kcal/mol@LB@Unfolding Energy - mRNA 0.691484 kcal/mol@LB@Unfolding Energy - sRNA 1.04836 kcal/mol@LB@@LB@Position - mRNA 236 -- 256@LB@Position - sRNA 70 -- 90@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 1 -- 7@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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