Input and runtime details for job 6038639 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6038639 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:35:57 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:19:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:35:30 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:35:37 CET 2018
	Job Completed		@ Fri Feb 16 21:35:51 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6038639&toolName=CopraRNA ( 30 days expiry )

Description of the job

IhtA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000117 Chlamydia trachomatis D/UW-3/CX

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
37	0.01294	0.2859	ct_591	sdhB	-7.06	0.139804	161 -- 176	18 -- 34	succinate dehydrogenase iron sulfur subunit	_____________160_______________177\n_____________\|_________________\|\n5'-AAA...AUUUA_____________UG__AAAUA...GGG-3'\n______________UAGAGGC_GUUCC__GG\n______________:\|\|\|:\|\|_:\|\|\|\|__\|\|\n______________GUCUUCG_UAAGG__CC\n3'-UUU...UCUCA_______A_____UA__GCAAU...GAA-5'\n_____________\|_________________\|\n_____________35________________17\n	161 -- 167	28 -- 34	-17.8156 kcal/mol	5.77845 kcal/mol	4.97803 kcal/mol	160 177@LB@ \| \|@LB@5'-AAA...AUUUA UG AAAUA...GGG-3'@LB@ UAGAGGC GUUCC GG@LB@ :\|\|\|:\|\| :\|\|\|\| \|\|@LB@ GUCUUCG UAAGG CC@LB@3'-UUU...UCUCA A UA GCAAU...GAA-5'@LB@ \| \|@LB@ 35 17@LB@@LB@@LB@Energy -7.06 kcal/mol@LB@Hybridization Energy -17.8156 kcal/mol@LB@Unfolding Energy - mRNA 5.77845 kcal/mol@LB@Unfolding Energy - sRNA 4.97803 kcal/mol@LB@@LB@Position - mRNA 161 -- 176@LB@Position - sRNA 18 -- 34@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 28 -- 34@LB@
58	0.02303	0.29	ct_184	N/A	-4.35	0.465673	5 -- 18	18 -- 35	Holliday junction resolvase	_____________4__________________19\n_____________\|__________________\|\n_______5'-ACGU_______AA_________AUUAA...UGC-3'\n______________UUAGAAG______CAUGG\n______________\|:\|\|\|\|\|______\|\|\|\|\|\n______________AGUCUUC______GUACC\n3'-UUU...AUCUC_______GAUAAG_____GCAAU...GAA-5'\n_____________\|__________________\|\n_____________36_________________17\n	5 -- 11	29 -- 35	-12.193 kcal/mol	2.86148 kcal/mol	4.97803 kcal/mol	4 19@LB@ \| \|@LB@ 5'-ACGU AA AUUAA...UGC-3'@LB@ UUAGAAG CAUGG@LB@ \|:\|\|\|\|\| \|\|\|\|\|@LB@ AGUCUUC GUACC@LB@3'-UUU...AUCUC GAUAAG GCAAU...GAA-5'@LB@ \| \|@LB@ 36 17@LB@@LB@@LB@Energy -4.35 kcal/mol@LB@Hybridization Energy -12.193 kcal/mol@LB@Unfolding Energy - mRNA 2.86148 kcal/mol@LB@Unfolding Energy - sRNA 4.97803 kcal/mol@LB@@LB@Position - mRNA 5 -- 18@LB@Position - sRNA 18 -- 35@LB@Position Seed - mRNA 5 -- 11@LB@Position Seed - sRNA 29 -- 35@LB@
75	0.02725	0.29	ct_536	dnaQ_2	-6.54	0.180881	173 -- 186	18 -- 31	DNA polymerase III subunit epsilon	_____________172____________187\n_____________\|______________\|\n5'-AGA...GAUUU_____U________AGAAC...GGU-3'\n______________GAGCU_UUUUAUGG\n______________:\|\|\|\|_\|\|::\|\|\|\|\n______________UUCGA_AAGGUACC\n3'-UUU...CAGUC_____U________GCAAU...GAA-5'\n_____________\|______________\|\n_____________32_____________17\n	180 -- 186	18 -- 24	-14.3298 kcal/mol	2.92947 kcal/mol	4.86077 kcal/mol	172 187@LB@ \| \|@LB@5'-AGA...GAUUU U AGAAC...GGU-3'@LB@ GAGCU UUUUAUGG@LB@ :\|\|\|\| \|\|::\|\|\|\|@LB@ UUCGA AAGGUACC@LB@3'-UUU...CAGUC U GCAAU...GAA-5'@LB@ \| \|@LB@ 32 17@LB@@LB@@LB@Energy -6.54 kcal/mol@LB@Hybridization Energy -14.3298 kcal/mol@LB@Unfolding Energy - mRNA 2.92947 kcal/mol@LB@Unfolding Energy - sRNA 4.86077 kcal/mol@LB@@LB@Position - mRNA 173 -- 186@LB@Position - sRNA 18 -- 31@LB@Position Seed - mRNA 180 -- 186@LB@Position Seed - sRNA 18 -- 24@LB@
85	0.03073	0.29	ct_522	rs3	-5.11	0.345416	189 -- 204	18 -- 34	30S ribosomal protein S3	_____________188_______________205\n_____________\|_________________\|\n5'-UGU...GGCAG_______AAGG______GUCAA...UCA-3'\n______________UAGGAGU_____UAUGG\n______________:\|\|:\|\|:_____:\|\|\|\|\n______________GUCUUCG_____GUACC\n3'-UUU...UCUCA_______AUAAG_____GCAAU...GAA-5'\n_____________\|_________________\|\n_____________35________________17\n	189 -- 195	28 -- 34	-12.0284 kcal/mol	1.93617 kcal/mol	4.97803 kcal/mol	188 205@LB@ \| \|@LB@5'-UGU...GGCAG AAGG GUCAA...UCA-3'@LB@ UAGGAGU UAUGG@LB@ :\|\|:\|\|: :\|\|\|\|@LB@ GUCUUCG GUACC@LB@3'-UUU...UCUCA AUAAG GCAAU...GAA-5'@LB@ \| \|@LB@ 35 17@LB@@LB@@LB@Energy -5.11 kcal/mol@LB@Hybridization Energy -12.0284 kcal/mol@LB@Unfolding Energy - mRNA 1.93617 kcal/mol@LB@Unfolding Energy - sRNA 4.97803 kcal/mol@LB@@LB@Position - mRNA 189 -- 204@LB@Position - sRNA 18 -- 34@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 28 -- 34@LB@
109	0.04235	0.3194	ct_002	gatC	-8.76	0.056639	255 -- 282	18 -- 44	aspartyl/glutamyl-tRNA amidotransferase subunit C	_____________254__________________________283\n_____________\|____________________________\|\n5'-GAC...AUGCU________________AUGCCCA_____AAGAG...CUA-3'\n______________GCAUUGGAGUUGGAAG_______CGUGG\n______________:\|\|:\|:\|\|\|\|::\|\|\|\|_______\|:\|\|\|\n______________UGUGAUCUCAGUCUUC_______GUACC\n3'-UUU...AUGUU________________GAUAAG______GCAAU...GAA-5'\n_____________\|____________________________\|\n_____________45___________________________17\n	261 -- 267	32 -- 38	-28.0671 kcal/mol	6.54471 kcal/mol	12.7603 kcal/mol	254 283@LB@ \| \|@LB@5'-GAC...AUGCU AUGCCCA AAGAG...CUA-3'@LB@ GCAUUGGAGUUGGAAG CGUGG@LB@ :\|\|:\|:\|\|\|\|::\|\|\|\| \|:\|\|\|@LB@ UGUGAUCUCAGUCUUC GUACC@LB@3'-UUU...AUGUU GAUAAG GCAAU...GAA-5'@LB@ \| \|@LB@ 45 17@LB@@LB@@LB@Energy -8.76 kcal/mol@LB@Hybridization Energy -28.0671 kcal/mol@LB@Unfolding Energy - mRNA 6.54471 kcal/mol@LB@Unfolding Energy - sRNA 12.7603 kcal/mol@LB@@LB@Position - mRNA 255 -- 282@LB@Position - sRNA 18 -- 44@LB@Position Seed - mRNA 261 -- 267@LB@Position Seed - sRNA 32 -- 38@LB@
124	0.04963	0.3262	ct_290	ptsN_1	-8.82	0.054773	1 -- 15	18 -- 32	PTS fructose transporter subunit IIA	______________1______________16\n______________\|______________\|\n___________5'-____G__________GAUGC...UUA-3'\n______________GAGG_UAUUUUGUGG\n______________\|\|:\|_\|\|\|\|:::\|\|\|\n______________CUUC_AUAAGGUACC\n3'-UUU...UCAGU____G__________GCAAU...GAA-5'\n_____________\|_______________\|\n_____________33______________17\n	9 -- 15	18 -- 24	-16.4794 kcal/mol	2.69105 kcal/mol	4.96566 kcal/mol	1 16@LB@ \| \|@LB@ 5'- G GAUGC...UUA-3'@LB@ GAGG UAUUUUGUGG@LB@ \|\|:\| \|\|\|\|:::\|\|\|@LB@ CUUC AUAAGGUACC@LB@3'-UUU...UCAGU G GCAAU...GAA-5'@LB@ \| \|@LB@ 33 17@LB@@LB@@LB@Energy -8.82 kcal/mol@LB@Hybridization Energy -16.4794 kcal/mol@LB@Unfolding Energy - mRNA 2.69105 kcal/mol@LB@Unfolding Energy - sRNA 4.96566 kcal/mol@LB@@LB@Position - mRNA 1 -- 15@LB@Position - sRNA 18 -- 32@LB@Position Seed - mRNA 9 -- 15@LB@Position Seed - sRNA 18 -- 24@LB@
127	0.0509	0.3262	ct_808	cafE	-7.80	0.095102	169 -- 204	18 -- 57	ribonuclease E	_____________168_________________________________________205\n_____________\|___________________________________________\|\n5'-GUU...GAGGA_______A______________CAU______ACG_________AAAAC...AAA-3'\n______________CUCCUGU____GAGCGC_AGAG___UUAGGA______CCAUGG\n______________\|:\|\|\|\|\|____:\|:\|:\|_\|\|\|\|___\|:\|\|:\|______\|\|\|\|\|\|\n______________GGGGACA____UUUGUG_UCUC___AGUCUU______GGUACC\n3'-UUU...GAAGG_______CAUG______A_____________CGAUAA______GCAAU...GAA-5'\n_____________\|___________________________________________\|\n_____________58__________________________________________17\n	169 -- 175	51 -- 57	-30.1775 kcal/mol	9.43695 kcal/mol	12.9362 kcal/mol	168 205@LB@ \| \|@LB@5'-GUU...GAGGA A CAU ACG AAAAC...AAA-3'@LB@ CUCCUGU GAGCGC AGAG UUAGGA CCAUGG@LB@ \|:\|\|\|\|\| :\|:\|:\| \|\|\|\| \|:\|\|:\| \|\|\|\|\|\|@LB@ GGGGACA UUUGUG UCUC AGUCUU GGUACC@LB@3'-UUU...GAAGG CAUG A CGAUAA GCAAU...GAA-5'@LB@ \| \|@LB@ 58 17@LB@@LB@@LB@Energy -7.80 kcal/mol@LB@Hybridization Energy -30.1775 kcal/mol@LB@Unfolding Energy - mRNA 9.43695 kcal/mol@LB@Unfolding Energy - sRNA 12.9362 kcal/mol@LB@@LB@Position - mRNA 169 -- 204@LB@Position - sRNA 18 -- 57@LB@Position Seed - mRNA 169 -- 175@LB@Position Seed - sRNA 51 -- 57@LB@
129	0.0523	0.3262	ct_872	pmpH	-6.76	0.162049	85 -- 103	18 -- 34	outer membrane protein PmpH	_____________84__________________104\n_____________\|___________________\|\n5'-AGA...CAAUA_______A___GG______AAGAU...AUG-3'\n______________UAGAAGU_UAU__CCAUGG\n______________:\|\|\|\|\|:_\|\|\|__\|\|\|\|\|\|\n______________GUCUUCG_AUA__GGUACC\n3'-UUU...UCUCA___________A_______GCAAU...GAA-5'\n_____________\|___________________\|\n_____________35__________________17\n	85 -- 91	28 -- 34	-16.9306 kcal/mol	5.18888 kcal/mol	4.97803 kcal/mol	84 104@LB@ \| \|@LB@5'-AGA...CAAUA A GG AAGAU...AUG-3'@LB@ UAGAAGU UAU CCAUGG@LB@ :\|\|\|\|\|: \|\|\| \|\|\|\|\|\|@LB@ GUCUUCG AUA GGUACC@LB@3'-UUU...UCUCA A GCAAU...GAA-5'@LB@ \| \|@LB@ 35 17@LB@@LB@@LB@Energy -6.76 kcal/mol@LB@Hybridization Energy -16.9306 kcal/mol@LB@Unfolding Energy - mRNA 5.18888 kcal/mol@LB@Unfolding Energy - sRNA 4.97803 kcal/mol@LB@@LB@Position - mRNA 85 -- 103@LB@Position - sRNA 18 -- 34@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 28 -- 34@LB@
136	0.05474	0.3262	ct_592	sdhA	-7.43	0.115894	38 -- 56	18 -- 34	succinate dehydrogenase flavoprotein subunit	_____________37__________________57\n_____________\|___________________\|\n5'-UCU...UGUUA___CU_____UU_______GUUUA...UGU-3'\n______________UAG__GCUGU__UCCAUGG\n______________:\|\|__\|\|\|:\|__\|\|\|\|\|\|\|\n______________GUC__CGAUA__AGGUACC\n3'-UUU...UCUCA___UU______________GCAAU...GAA-5'\n_____________\|___________________\|\n_____________35__________________17\n	50 -- 56	18 -- 24	-18.2308 kcal/mol	5.82664 kcal/mol	4.97803 kcal/mol	37 57@LB@ \| \|@LB@5'-UCU...UGUUA CU UU GUUUA...UGU-3'@LB@ UAG GCUGU UCCAUGG@LB@ :\|\| \|\|\|:\| \|\|\|\|\|\|\|@LB@ GUC CGAUA AGGUACC@LB@3'-UUU...UCUCA UU GCAAU...GAA-5'@LB@ \| \|@LB@ 35 17@LB@@LB@@LB@Energy -7.43 kcal/mol@LB@Hybridization Energy -18.2308 kcal/mol@LB@Unfolding Energy - mRNA 5.82664 kcal/mol@LB@Unfolding Energy - sRNA 4.97803 kcal/mol@LB@@LB@Position - mRNA 38 -- 56@LB@Position - sRNA 18 -- 34@LB@Position Seed - mRNA 50 -- 56@LB@Position Seed - sRNA 18 -- 24@LB@
142	0.0566	0.3262	ct_284	N/A	-6.95	0.147726	278 -- 292	18 -- 30	phospholipase D	_____________277_____________293\n_____________\|_______________\|\n5'-AUC...ACUAU_________GG____AGACAAAA-3'\n______________AGCUAUUCC__AUGG\n______________\|\|\|\|\|\|\|\|\|__\|\|\|\|\n______________UCGAUAAGG__UACC\n3'-UUU...AGUCU_______________GCAAU...GAA-5'\n_____________\|_______________\|\n_____________31______________17\n	280 -- 286	22 -- 28	-16.5616 kcal/mol	4.87795 kcal/mol	4.73403 kcal/mol	277 293@LB@ \| \|@LB@5'-AUC...ACUAU GG AGACAAAA-3'@LB@ AGCUAUUCC AUGG@LB@ \|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ UCGAUAAGG UACC@LB@3'-UUU...AGUCU GCAAU...GAA-5'@LB@ \| \|@LB@ 31 17@LB@@LB@@LB@Energy -6.95 kcal/mol@LB@Hybridization Energy -16.5616 kcal/mol@LB@Unfolding Energy - mRNA 4.87795 kcal/mol@LB@Unfolding Energy - sRNA 4.73403 kcal/mol@LB@@LB@Position - mRNA 278 -- 292@LB@Position - sRNA 18 -- 30@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 22 -- 28@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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