Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
9	4.743e-05	0.01855	b1204	pth	-17.38	0.004387	162 -- 190	43 -- 74	peptidyl-tRNA hydrolase	_____________161_______________________________191\n_____________\|_________________________________\|\n5'-UGG...AGUAA________________A_____ACA________ACAAA...AGC-3'\n______________ACUACGCGC__CAGUU___UGU___CACUCAGG\n______________\|\|\|\|\|:\|:\|__\|\|:\|\|___\|\|\|___\|\|\|\|\|\|\|\|\n______________UGAUGUGUG__GUUAA___ACA___GUGAGUCC\n3'-UUU...GUUUC_________UC_____GCA___AA_________CCUAA...GCA-5'\n_____________\|_________________________________\|\n_____________75________________________________42\n	184 -- 190	43 -- 49	-27.969 kcal/mol	6.24318 kcal/mol	4.35222 kcal/mol	161 191@LB@ \| \|@LB@5'-UGG...AGUAA A ACA ACAAA...AGC-3'@LB@ ACUACGCGC CAGUU UGU CACUCAGG@LB@ \|\|\|\|\|:\|:\| \|\|:\|\| \|\|\| \|\|\|\|\|\|\|\|@LB@ UGAUGUGUG GUUAA ACA GUGAGUCC@LB@3'-UUU...GUUUC UC GCA AA CCUAA...GCA-5'@LB@ \| \|@LB@ 75 42@LB@@LB@@LB@Energy -17.38 kcal/mol@LB@Hybridization Energy -27.969 kcal/mol@LB@Unfolding Energy - mRNA 6.24318 kcal/mol@LB@Unfolding Energy - sRNA 4.35222 kcal/mol@LB@@LB@Position - mRNA 162 -- 190@LB@Position - sRNA 43 -- 74@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 43 -- 49@LB@
169	0.008352	0.1723	b1222	narX	-15.18	0.018797	58 -- 74	61 -- 75	sensory histidine kinase in two-component regulatory system with NarL	_____________57________________75\n_____________\|_________________\|\n5'-AAA...AAAGC_____AC__________AUAAG...CUG-3'\n______________GACUA__CACACGGCAA\n______________\|\|\|\|\|__\|\|\|\|\|:\|\|\|\|\n______________CUGAU__GUGUGUCGUU\n3'-UUU...CGUUU_________________AAGCA...GCA-5'\n_____________\|_________________\|\n_____________76________________60\n	68 -- 74	61 -- 67	-20.5903 kcal/mol	2.1582 kcal/mol	3.25399 kcal/mol	57 75@LB@ \| \|@LB@5'-AAA...AAAGC AC AUAAG...CUG-3'@LB@ GACUA CACACGGCAA@LB@ \|\|\|\|\| \|\|\|\|\|:\|\|\|\|@LB@ CUGAU GUGUGUCGUU@LB@3'-UUU...CGUUU AAGCA...GCA-5'@LB@ \| \|@LB@ 76 60@LB@@LB@@LB@Energy -15.18 kcal/mol@LB@Hybridization Energy -20.5903 kcal/mol@LB@Unfolding Energy - mRNA 2.1582 kcal/mol@LB@Unfolding Energy - sRNA 3.25399 kcal/mol@LB@@LB@Position - mRNA 58 -- 74@LB@Position - sRNA 61 -- 75@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 61 -- 67@LB@
46	0.001357	0.1038	b1235	rssB	-13.33	0.053430	184 -- 194	40 -- 50	PcnB-degradosome interaction factor; response regulator	_____________183_________195\n_____________\|___________\|\n5'-AAU...AAAGC__G________GAGAA...CAA-3'\n______________CA_UCAGGGGA\n______________\|\|_\|\|\|\|\|\|\|\|\n______________GU_AGUCCCCU\n3'-UUU...ACAAA__G________AAAGG...GCA-5'\n_____________\|___________\|\n_____________51__________39\n	188 -- 194	40 -- 46	-19.1981 kcal/mol	4.11697 kcal/mol	1.75243 kcal/mol	183 195@LB@ \| \|@LB@5'-AAU...AAAGC G GAGAA...CAA-3'@LB@ CA UCAGGGGA@LB@ \|\| \|\|\|\|\|\|\|\|@LB@ GU AGUCCCCU@LB@3'-UUU...ACAAA G AAAGG...GCA-5'@LB@ \| \|@LB@ 51 39@LB@@LB@@LB@Energy -13.33 kcal/mol@LB@Hybridization Energy -19.1981 kcal/mol@LB@Unfolding Energy - mRNA 4.11697 kcal/mol@LB@Unfolding Energy - sRNA 1.75243 kcal/mol@LB@@LB@Position - mRNA 184 -- 194@LB@Position - sRNA 40 -- 50@LB@Position Seed - mRNA 188 -- 194@LB@Position Seed - sRNA 40 -- 46@LB@
176	0.008803	0.1747	b1242	ychE	-11.39	0.137124	41 -- 64	45 -- 75	UPF0056 family inner membrane protein	_____________40______________________________65\n_____________\|_______________________________\|\n5'-CAU...UUGGU____________________________A__ACUAG...GCA-3'\n______________GGCUACAU_UAGCA______UGUUUUGC_CA\n______________\|:\|\|\|\|\|:_:\|\|\|\|______\|\|\|\|\|::\|_\|\|\n______________CUGAUGUG_GUCGU______ACAAAGUG_GU\n3'-UUU...CGUUU________U_____UAAGCA________A__CCCCU...GCA-5'\n_____________\|_______________________________\|\n_____________76______________________________44\n	55 -- 61	48 -- 54	-19.9041 kcal/mol	4.77357 kcal/mol	3.74078 kcal/mol	40 65@LB@ \| \|@LB@5'-CAU...UUGGU A ACUAG...GCA-3'@LB@ GGCUACAU UAGCA UGUUUUGC CA@LB@ \|:\|\|\|\|\|: :\|\|\|\| \|\|\|\|\|::\| \|\|@LB@ CUGAUGUG GUCGU ACAAAGUG GU@LB@3'-UUU...CGUUU U UAAGCA A CCCCU...GCA-5'@LB@ \| \|@LB@ 76 44@LB@@LB@@LB@Energy -11.39 kcal/mol@LB@Hybridization Energy -19.9041 kcal/mol@LB@Unfolding Energy - mRNA 4.77357 kcal/mol@LB@Unfolding Energy - sRNA 3.74078 kcal/mol@LB@@LB@Position - mRNA 41 -- 64@LB@Position - sRNA 45 -- 75@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 48 -- 54@LB@
187	0.009506	0.1776	b1243	oppA	-10.52	0.199375	11 -- 17	40 -- 46	oligopeptide ABC transporter periplasmic binding protein	_____________10______18\n_____________\|_______\|\n5'-UGU...CUCGA_______GACAC...UCA-3'\n______________CAGGGGA\n______________\|\|\|\|\|\|\|\n______________GUCCCCU\n3'-UUU...AGUGA_______AAAGG...GCA-5'\n_____________\|_______\|\n_____________47______39\n	11 -- 17	40 -- 46	-13.6251 kcal/mol	1.75344 kcal/mol	1.35582 kcal/mol	10 18@LB@ \| \|@LB@5'-UGU...CUCGA GACAC...UCA-3'@LB@ CAGGGGA@LB@ \|\|\|\|\|\|\|@LB@ GUCCCCU@LB@3'-UUU...AGUGA AAAGG...GCA-5'@LB@ \| \|@LB@ 47 39@LB@@LB@@LB@Energy -10.52 kcal/mol@LB@Hybridization Energy -13.6251 kcal/mol@LB@Unfolding Energy - mRNA 1.75344 kcal/mol@LB@Unfolding Energy - sRNA 1.35582 kcal/mol@LB@@LB@Position - mRNA 11 -- 17@LB@Position - sRNA 40 -- 46@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 40 -- 46@LB@
124	0.005471	0.1553	b1249	clsA	-8.47	0.418250	164 -- 196	34 -- 71	cardiolipin synthase 1	_____________163____________________________________197\n_____________\|______________________________________\|\n5'-GUC...CGUAA__U____A____G__C_______AAA____________AUCUA...CAG-3'\n______________AC_CAUA_CAAU_CG___UUUCA______GGAUUUCUA\n______________\|\|_\|\|:\|_\|\|\|\|_\|\|___\|\|\|\|\|______\|\|\|\|\|\|\|:\|\n______________UG_GUGU_GUUA_GC___AAAGU______CCUAAAGGU\n3'-UUU...UCUGA__U____C____A__AAC_____GAGUCC_________ACGAA...GCA-5'\n_____________\|______________________________________\|\n_____________72_____________________________________33\n	190 -- 196	34 -- 40	-20.3655 kcal/mol	7.10457 kcal/mol	4.79237 kcal/mol	163 197@LB@ \| \|@LB@5'-GUC...CGUAA U A G C AAA AUCUA...CAG-3'@LB@ AC CAUA CAAU CG UUUCA GGAUUUCUA@LB@ \|\| \|\|:\| \|\|\|\| \|\| \|\|\|\|\| \|\|\|\|\|\|\|:\|@LB@ UG GUGU GUUA GC AAAGU CCUAAAGGU@LB@3'-UUU...UCUGA U C A AAC GAGUCC ACGAA...GCA-5'@LB@ \| \|@LB@ 72 33@LB@@LB@@LB@Energy -8.47 kcal/mol@LB@Hybridization Energy -20.3655 kcal/mol@LB@Unfolding Energy - mRNA 7.10457 kcal/mol@LB@Unfolding Energy - sRNA 4.79237 kcal/mol@LB@@LB@Position - mRNA 164 -- 196@LB@Position - sRNA 34 -- 71@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 34 -- 40@LB@
89	0.003963	0.1538	b1252	tonB	-10.58	0.194225	25 -- 43	64 -- 82	membrane spanning protein in TonB-ExbB-ExbD transport complex	_____________24___________________44\n_____________\|____________________\|\n5'-AGU...UGAAA_________U__________AAAGA...AUC-3'\n______________GGGCGAAGA_CUGCA_ACGG\n______________\|\|\|\|:\|\|\|\|_\|\|:\|\|_\|\|:\|\n______________CCCGUUUCU_GAUGU_UGUC\n3'-UUU...CUCUA_______________G____GUUAA...GCA-5'\n_____________\|____________________\|\n_____________83___________________63\n	25 -- 31	76 -- 82	-25.6168 kcal/mol	4.83236 kcal/mol	10.2054 kcal/mol	24 44@LB@ \| \|@LB@5'-AGU...UGAAA U AAAGA...AUC-3'@LB@ GGGCGAAGA CUGCA ACGG@LB@ \|\|\|\|:\|\|\|\| \|\|:\|\| \|\|:\|@LB@ CCCGUUUCU GAUGU UGUC@LB@3'-UUU...CUCUA G GUUAA...GCA-5'@LB@ \| \|@LB@ 83 63@LB@@LB@@LB@Energy -10.58 kcal/mol@LB@Hybridization Energy -25.6168 kcal/mol@LB@Unfolding Energy - mRNA 4.83236 kcal/mol@LB@Unfolding Energy - sRNA 10.2054 kcal/mol@LB@@LB@Position - mRNA 25 -- 43@LB@Position - sRNA 64 -- 82@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 76 -- 82@LB@
158	0.007677	0.1668	b1267	yciO	-9.92	0.252655	228 -- 242	61 -- 75	putative RNA binding protein	_____________227_____________243\n_____________\|_______________\|\n5'-GCG...AUCCU___A__C________CGUCU...CAA-3'\n______________GAU_AC_CACAGCAA\n______________\|\|:_\|\|_\|\|\|\|\|\|\|\|\n______________CUG_UG_GUGUCGUU\n3'-UUU...CGUUU___A__U________AAGCA...GCA-5'\n_____________\|_______________\|\n_____________76______________60\n	236 -- 242	61 -- 67	-14.6343 kcal/mol	1.46218 kcal/mol	3.25399 kcal/mol	227 243@LB@ \| \|@LB@5'-GCG...AUCCU A C CGUCU...CAA-3'@LB@ GAU AC CACAGCAA@LB@ \|\|: \|\| \|\|\|\|\|\|\|\|@LB@ CUG UG GUGUCGUU@LB@3'-UUU...CGUUU A U AAGCA...GCA-5'@LB@ \| \|@LB@ 76 60@LB@@LB@@LB@Energy -9.92 kcal/mol@LB@Hybridization Energy -14.6343 kcal/mol@LB@Unfolding Energy - mRNA 1.46218 kcal/mol@LB@Unfolding Energy - sRNA 3.25399 kcal/mol@LB@@LB@Position - mRNA 228 -- 242@LB@Position - sRNA 61 -- 75@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 61 -- 67@LB@
39	0.0006857	0.06187	b1305	pspB	-16.08	0.010660	268 -- 296	48 -- 75	psp operon transcription co-activator	_____________267____________________________297\n_____________\|______________________________\|\n5'-AUU...GUUAU_______U______________CUGG____AGUG-3'\n______________GGCUGCA_UACAGCAAU_CGUU____UCGC\n______________\|:\|\|:\|\|_:\|\|\|\|\|\|\|\|_\|\|\|\|____\|\|:\|\n______________CUGAUGU_GUGUCGUUA_GCAA____AGUG\n3'-UUU...CGUUU_________________A____CAA_____AGUCC...GCA-5'\n_____________\|______________________________\|\n_____________76_____________________________47\n	278 -- 284	60 -- 66	-29.5086 kcal/mol	9.74074 kcal/mol	3.68696 kcal/mol	267 297@LB@ \| \|@LB@5'-AUU...GUUAU U CUGG AGUG-3'@LB@ GGCUGCA UACAGCAAU CGUU UCGC@LB@ \|:\|\|:\|\| :\|\|\|\|\|\|\|\| \|\|\|\| \|\|:\|@LB@ CUGAUGU GUGUCGUUA GCAA AGUG@LB@3'-UUU...CGUUU A CAA AGUCC...GCA-5'@LB@ \| \|@LB@ 76 47@LB@@LB@@LB@Energy -16.08 kcal/mol@LB@Hybridization Energy -29.5086 kcal/mol@LB@Unfolding Energy - mRNA 9.74074 kcal/mol@LB@Unfolding Energy - sRNA 3.68696 kcal/mol@LB@@LB@Position - mRNA 268 -- 296@LB@Position - sRNA 48 -- 75@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 60 -- 66@LB@
66	0.002697	0.1417	b1306	pspC	-11.06	0.158336	44 -- 64	55 -- 75	psp operon transcription co-activator	_____________43_____________________65\n_____________\|______________________\|\n5'-CCG...GUUAU_______U______________CUGGU...CGG-3'\n______________GGCUGCA_UACAGCAAU_CGUU\n______________\|:\|\|:\|\|_:\|\|\|\|\|\|\|\|_\|\|\|\|\n______________CUGAUGU_GUGUCGUUA_GCAA\n3'-UUU...CGUUU_________________A____CAAAG...GCA-5'\n_____________\|______________________\|\n_____________76_____________________54\n	54 -- 60	60 -- 66	-24.9269 kcal/mol	10.2549 kcal/mol	3.6092 kcal/mol	43 65@LB@ \| \|@LB@5'-CCG...GUUAU U CUGGU...CGG-3'@LB@ GGCUGCA UACAGCAAU CGUU@LB@ \|:\|\|:\|\| :\|\|\|\|\|\|\|\| \|\|\|\|@LB@ CUGAUGU GUGUCGUUA GCAA@LB@3'-UUU...CGUUU A CAAAG...GCA-5'@LB@ \| \|@LB@ 76 54@LB@@LB@@LB@Energy -11.06 kcal/mol@LB@Hybridization Energy -24.9269 kcal/mol@LB@Unfolding Energy - mRNA 10.2549 kcal/mol@LB@Unfolding Energy - sRNA 3.6092 kcal/mol@LB@@LB@Position - mRNA 44 -- 64@LB@Position - sRNA 55 -- 75@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 60 -- 66@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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