Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
55	0.001955	0.1223	b0840	deoR	-10.34	0.214583	216 -- 260	60 -- 103	deoxyribose-5-phosphate-inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx	_____________215____________________________________________261\n_____________\|______________________________________________\|\n5'-GUG...GUCGC_______G________C__C__CU_____A____AA__________AAGUU...UUU-3'\n______________GAAGAGC_UAUCG_GG_AG_UG__GCAAG_AUUA__ACGCAGCGAU\n______________:\|\|:\|\|\|_:\|\|\|\|_\|\|_\|\|_\|\|__\|\|\|\|:_\|:\|\|__\|\|:\|\|\|\|:\|\|\n______________UUUUUCG_GUAGC_CC_UC_AC__CGUUU_UGAU__UGUGUCGUUA\n_________3'-UU_______G_____A__C__U__C______C____G___________AGCAA...GCA-5'\n_____________\|______________________________________________\|\n_____________104____________________________________________59\n	254 -- 260	60 -- 66	-39.4084 kcal/mol	12.9673 kcal/mol	16.1071 kcal/mol	215 261@LB@ \| \|@LB@5'-GUG...GUCGC G C C CU A AA AAGUU...UUU-3'@LB@ GAAGAGC UAUCG GG AG UG GCAAG AUUA ACGCAGCGAU@LB@ :\|\|:\|\|\| :\|\|\|\| \|\| \|\| \|\| \|\|\|\|: \|:\|\| \|\|:\|\|\|\|:\|\|@LB@ UUUUUCG GUAGC CC UC AC CGUUU UGAU UGUGUCGUUA@LB@ 3'-UU G A C U C C G AGCAA...GCA-5'@LB@ \| \|@LB@ 104 59@LB@@LB@@LB@Energy -10.34 kcal/mol@LB@Hybridization Energy -39.4084 kcal/mol@LB@Unfolding Energy - mRNA 12.9673 kcal/mol@LB@Unfolding Energy - sRNA 16.1071 kcal/mol@LB@@LB@Position - mRNA 216 -- 260@LB@Position - sRNA 60 -- 103@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 60 -- 66@LB@
75	0.003119	0.1452	b0871	poxB	-11.12	0.154817	181 -- 203	32 -- 50	pyruvate dehydrogenase thiamine triphosphate-binding FAD-binding	_____________180_____________________204\n_____________\|_______________________\|\n5'-CCG...UAUCA________AGAU___GAA_____AAACA...GUG-3'\n______________CAUUCAGG____GGA___CCAUG\n______________\|\|:\|\|\|\|\|____\|\|\|___\|\|\|\|\|\n______________GUGAGUCC____CCU___GGUAC\n3'-UUU...ACAAA_______________AAA_____GAAUA...GCA-5'\n_____________\|_______________________\|\n_____________51______________________31\n	182 -- 188	43 -- 49	-21.2585 kcal/mol	5.84423 kcal/mol	4.29868 kcal/mol	180 204@LB@ \| \|@LB@5'-CCG...UAUCA AGAU GAA AAACA...GUG-3'@LB@ CAUUCAGG GGA CCAUG@LB@ \|\|:\|\|\|\|\| \|\|\| \|\|\|\|\|@LB@ GUGAGUCC CCU GGUAC@LB@3'-UUU...ACAAA AAA GAAUA...GCA-5'@LB@ \| \|@LB@ 51 31@LB@@LB@@LB@Energy -11.12 kcal/mol@LB@Hybridization Energy -21.2585 kcal/mol@LB@Unfolding Energy - mRNA 5.84423 kcal/mol@LB@Unfolding Energy - sRNA 4.29868 kcal/mol@LB@@LB@Position - mRNA 181 -- 203@LB@Position - sRNA 32 -- 50@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 43 -- 49@LB@
64	0.002655	0.1417	b0876	ybjD	-10.33	0.215429	171 -- 200	39 -- 78	putative OLD family ATP-dependent endonuclease; DUF2813 family protein	_____________170________________________________________201\n_____________\|__________________________________________\|\n5'-CGU...GCGUA______AAGU_____________________UGG________AUGAU...GGG-3'\n______________AAGGAU____CACAUA___________UUUC____AGGGGAU\n______________\|\|:\|\|:____\|\|\|\|:\|___________\|\|\|\|____\|\|\|\|\|\|\|\n______________UUUCUG____GUGUGU___________AAAG____UCCCCUA\n3'-UUU...ACCCG______AU________CGUUAAGCAAC____UGAG_______AAGGU...GCA-5'\n_____________\|__________________________________________\|\n_____________79_________________________________________38\n	194 -- 200	39 -- 45	-20.0974 kcal/mol	4.99339 kcal/mol	4.77966 kcal/mol	170 201@LB@ \| \|@LB@5'-CGU...GCGUA AAGU UGG AUGAU...GGG-3'@LB@ AAGGAU CACAUA UUUC AGGGGAU@LB@ \|\|:\|\|: \|\|\|\|:\| \|\|\|\| \|\|\|\|\|\|\|@LB@ UUUCUG GUGUGU AAAG UCCCCUA@LB@3'-UUU...ACCCG AU CGUUAAGCAAC UGAG AAGGU...GCA-5'@LB@ \| \|@LB@ 79 38@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -20.0974 kcal/mol@LB@Unfolding Energy - mRNA 4.99339 kcal/mol@LB@Unfolding Energy - sRNA 4.77966 kcal/mol@LB@@LB@Position - mRNA 171 -- 200@LB@Position - sRNA 39 -- 78@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 39 -- 45@LB@
60	0.002324	0.1363	b0881	clpS	-11.74	0.117068	9 -- 15	40 -- 46	regulatory protein for ClpA substrate specificity	_____________8_______16\n_____________\|_______\|\n___5'-GCAUUUGA_______CAAGG...UCA-3'\n______________CAGGGGA\n______________\|\|\|\|\|\|\|\n______________GUCCCCU\n3'-UUU...AGUGA_______AAAGG...GCA-5'\n_____________\|_______\|\n_____________47______39\n	9 -- 15	40 -- 46	-13.2936 kcal/mol	0.199257 kcal/mol	1.35582 kcal/mol	8 16@LB@ \| \|@LB@ 5'-GCAUUUGA CAAGG...UCA-3'@LB@ CAGGGGA@LB@ \|\|\|\|\|\|\|@LB@ GUCCCCU@LB@3'-UUU...AGUGA AAAGG...GCA-5'@LB@ \| \|@LB@ 47 39@LB@@LB@@LB@Energy -11.74 kcal/mol@LB@Hybridization Energy -13.2936 kcal/mol@LB@Unfolding Energy - mRNA 0.199257 kcal/mol@LB@Unfolding Energy - sRNA 1.35582 kcal/mol@LB@@LB@Position - mRNA 9 -- 15@LB@Position - sRNA 40 -- 46@LB@Position Seed - mRNA 9 -- 15@LB@Position Seed - sRNA 40 -- 46@LB@
113	0.004991	0.1545	b0888	trxB	-9.59	0.285839	209 -- 223	61 -- 75	thioredoxin reductase FAD/NAD(P)-binding	_____________208_____________224\n_____________\|_______________\|\n5'-AAC...GGCAC___CAA_________ACUGC...UGC-3'\n______________GAC___ACACAGUAA\n______________\|\|\|___\|\|\|\|\|\|:\|\|\n______________CUG___UGUGUCGUU\n3'-UUU...CGUUU___AUG_________AAGCA...GCA-5'\n_____________\|_______________\|\n_____________76______________60\n	217 -- 223	61 -- 67	-15.4977 kcal/mol	2.65453 kcal/mol	3.25399 kcal/mol	208 224@LB@ \| \|@LB@5'-AAC...GGCAC CAA ACUGC...UGC-3'@LB@ GAC ACACAGUAA@LB@ \|\|\| \|\|\|\|\|\|:\|\|@LB@ CUG UGUGUCGUU@LB@3'-UUU...CGUUU AUG AAGCA...GCA-5'@LB@ \| \|@LB@ 76 60@LB@@LB@@LB@Energy -9.59 kcal/mol@LB@Hybridization Energy -15.4977 kcal/mol@LB@Unfolding Energy - mRNA 2.65453 kcal/mol@LB@Unfolding Energy - sRNA 3.25399 kcal/mol@LB@@LB@Position - mRNA 209 -- 223@LB@Position - sRNA 61 -- 75@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 61 -- 67@LB@
139	0.006425	0.1605	b0924	mukB	-11.72	0.118276	203 -- 227	45 -- 75	chromosome condensin MukBEF ATPase and DNA-binding subunit	_____________202_____________________________228\n_____________\|_______________________________\|\n5'-UGG...AUAAU_____A_________A_______________CUGAC...CUG-3'\n______________GAUUG_ACGCGGUAA_______UUUCGCUCA\n______________\|\|:\|:_\|\|:\|:\|:\|\|_______\|\|\|\|:\|\|\|\|\n______________CUGAU_UGUGUCGUU_______AAAGUGAGU\n3'-UUU...CGUUU_____G_________AAGCAAC_________CCCCU...GCA-5'\n_____________\|_______________________________\|\n_____________76______________________________44\n	221 -- 227	45 -- 51	-22.3255 kcal/mol	6.8688 kcal/mol	3.74078 kcal/mol	202 228@LB@ \| \|@LB@5'-UGG...AUAAU A A CUGAC...CUG-3'@LB@ GAUUG ACGCGGUAA UUUCGCUCA@LB@ \|\|:\|: \|\|:\|:\|:\|\| \|\|\|\|:\|\|\|\|@LB@ CUGAU UGUGUCGUU AAAGUGAGU@LB@3'-UUU...CGUUU G AAGCAAC CCCCU...GCA-5'@LB@ \| \|@LB@ 76 44@LB@@LB@@LB@Energy -11.72 kcal/mol@LB@Hybridization Energy -22.3255 kcal/mol@LB@Unfolding Energy - mRNA 6.8688 kcal/mol@LB@Unfolding Energy - sRNA 3.74078 kcal/mol@LB@@LB@Position - mRNA 203 -- 227@LB@Position - sRNA 45 -- 75@LB@Position Seed - mRNA 221 -- 227@LB@Position Seed - sRNA 45 -- 51@LB@
189	0.009605	0.1776	b0927	ycbL	-15.21	0.018444	14 -- 51	33 -- 78	putative metal-binding enzyme	_____________13__________________________________________________52\n_____________\|___________________________________________________\|\n5'-GGA...GAAGA________U_____UAAAG__________________CGAU__________AUUGA...GCG-3'\n______________AAAG_CUA_CACAC________________GC_CAGG____GGAUUUCCAU\n______________\|\|\|\|_\|\|\|_\|\|\|\|\|________________:\|_\|\|\|\|____\|\|\|\|\|\|\|\|\|\|\n______________UUUC_GAU_GUGUG________________UG_GUCC____CCUAAAGGUA\n3'-UUU...ACCCG____U_________UCGUUAAGCAACAAAG__A__________________CGAAU...GCA-5'\n_____________\|___________________________________________________\|\n_____________79__________________________________________________32\n	45 -- 51	33 -- 39	-26.8189 kcal/mol	6.79395 kcal/mol	4.81602 kcal/mol	13 52@LB@ \| \|@LB@5'-GGA...GAAGA U UAAAG CGAU AUUGA...GCG-3'@LB@ AAAG CUA CACAC GC CAGG GGAUUUCCAU@LB@ \|\|\|\| \|\|\| \|\|\|\|\| :\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|@LB@ UUUC GAU GUGUG UG GUCC CCUAAAGGUA@LB@3'-UUU...ACCCG U UCGUUAAGCAACAAAG A CGAAU...GCA-5'@LB@ \| \|@LB@ 79 32@LB@@LB@@LB@Energy -15.21 kcal/mol@LB@Hybridization Energy -26.8189 kcal/mol@LB@Unfolding Energy - mRNA 6.79395 kcal/mol@LB@Unfolding Energy - sRNA 4.81602 kcal/mol@LB@@LB@Position - mRNA 14 -- 51@LB@Position - sRNA 33 -- 78@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 33 -- 39@LB@
91	0.00412	0.1538	b0931	pncB	-13.41	0.051237	255 -- 284	48 -- 77	nicotinate phosphoribosyltransferase	_____________254______________________________285\n_____________\|________________________________\|\n5'-AUG...CUUAU__________AU_______GC___________CACUA...UGC-3'\n______________AAG_UUGCAU__GCAGCAA__CG_UGUUUCAU\n______________\|\|\|_:\|:\|\|:__:\|\|\|\|\|\|__\|\|_\|\|\|\|\|\|\|:\n______________UUC_GAUGUG__UGUCGUU__GC_ACAAAGUG\n3'-UUU...CCCGU___U_______________AA__A________AGUCC...GCA-5'\n_____________\|________________________________\|\n_____________78_______________________________47\n	278 -- 284	48 -- 54	-26.8209 kcal/mol	9.72536 kcal/mol	3.68729 kcal/mol	254 285@LB@ \| \|@LB@5'-AUG...CUUAU AU GC CACUA...UGC-3'@LB@ AAG UUGCAU GCAGCAA CG UGUUUCAU@LB@ \|\|\| :\|:\|\|: :\|\|\|\|\|\| \|\| \|\|\|\|\|\|\|:@LB@ UUC GAUGUG UGUCGUU GC ACAAAGUG@LB@3'-UUU...CCCGU U AA A AGUCC...GCA-5'@LB@ \| \|@LB@ 78 47@LB@@LB@@LB@Energy -13.41 kcal/mol@LB@Hybridization Energy -26.8209 kcal/mol@LB@Unfolding Energy - mRNA 9.72536 kcal/mol@LB@Unfolding Energy - sRNA 3.68729 kcal/mol@LB@@LB@Position - mRNA 255 -- 284@LB@Position - sRNA 48 -- 77@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 48 -- 54@LB@
177	0.008834	0.1747	b0932	pepN	-9.31	0.316043	123 -- 145	58 -- 78	aminopeptidase N	_____________122_____________________146\n_____________\|_______________________\|\n5'-GUA...AAGAU_____UU_______GC_______ACGCA...AGA-3'\n______________GAAGA__CUGCAUA__GCGAUUU\n______________:\|\|\|\|__\|\|:\|\|:\|__\|\|:\|\|\|:\n______________UUUCU__GAUGUGU__CGUUAAG\n3'-UUU...ACCCG______________GU_______CAACA...GCA-5'\n_____________\|_______________________\|\n_____________79______________________57\n	139 -- 145	58 -- 64	-18.0038 kcal/mol	5.38317 kcal/mol	3.31018 kcal/mol	122 146@LB@ \| \|@LB@5'-GUA...AAGAU UU GC ACGCA...AGA-3'@LB@ GAAGA CUGCAUA GCGAUUU@LB@ :\|\|\|\| \|\|:\|\|:\| \|\|:\|\|\|:@LB@ UUUCU GAUGUGU CGUUAAG@LB@3'-UUU...ACCCG GU CAACA...GCA-5'@LB@ \| \|@LB@ 79 57@LB@@LB@@LB@Energy -9.31 kcal/mol@LB@Hybridization Energy -18.0038 kcal/mol@LB@Unfolding Energy - mRNA 5.38317 kcal/mol@LB@Unfolding Energy - sRNA 3.31018 kcal/mol@LB@@LB@Position - mRNA 123 -- 145@LB@Position - sRNA 58 -- 78@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 58 -- 64@LB@
149	0.006962	0.1638	b0935	ssuD	-8.45	0.421381	102 -- 123	48 -- 71	alkanesulfonate monooxygenase FMNH(2)-dependent	_____________101______________________124\n_____________\|________________________\|\n5'-AAA...CAGCA___A_______A____________GAAAA...AAC-3'\n______________GCA_ACGGCAG_UC__UGUUUUAU\n______________:\|\|_\|\|:\|\|\|:_\|\|__\|\|\|\|\|:\|:\n______________UGU_UGUCGUU_AG__ACAAAGUG\n3'-UUU...UCUGA___G_______A__CA________AGUCC...GCA-5'\n_____________\|________________________\|\n_____________72_______________________47\n	117 -- 123	48 -- 54	-17.2917 kcal/mol	5.19453 kcal/mol	3.65243 kcal/mol	101 124@LB@ \| \|@LB@5'-AAA...CAGCA A A GAAAA...AAC-3'@LB@ GCA ACGGCAG UC UGUUUUAU@LB@ :\|\| \|\|:\|\|\|: \|\| \|\|\|\|\|:\|:@LB@ UGU UGUCGUU AG ACAAAGUG@LB@3'-UUU...UCUGA G A CA AGUCC...GCA-5'@LB@ \| \|@LB@ 72 47@LB@@LB@@LB@Energy -8.45 kcal/mol@LB@Hybridization Energy -17.2917 kcal/mol@LB@Unfolding Energy - mRNA 5.19453 kcal/mol@LB@Unfolding Energy - sRNA 3.65243 kcal/mol@LB@@LB@Position - mRNA 102 -- 123@LB@Position - sRNA 48 -- 71@LB@Position Seed - mRNA 117 -- 123@LB@Position Seed - sRNA 48 -- 54@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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