Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
68	0.002787	0.1417	b3001	gpr	-15.31	0.017381	36 -- 59	44 -- 69	L-glyceraldehyde 3-phosphate reductase		_____________35__________________________60\n_____________\|___________________________\|\n5'-UCC...GUUAA________________CAC________UUGGU...GGC-3'\n______________ACGCGGCA__UCGUUG___UC_CUCAG\n______________\|\|:\|:\|\|\|__\|\|\|\|\|\|___\|\|_\|\|\|\|\|\n______________UGUGUCGU__AGCAAC___AG_GAGUC\n3'-UUU...UGAUG________UA______AA___U_____CCCUA...GCA-5'\n_____________\|___________________________\|\n_____________70__________________________43\n	37 -- 43	62 -- 68	-25.5933 kcal/mol	6.1914 kcal/mol	4.09554 kcal/mol	35 60@LB@ \| \|@LB@5'-UCC...GUUAA CAC UUGGU...GGC-3'@LB@ ACGCGGCA UCGUUG UC CUCAG@LB@ \|\|:\|:\|\|\| \|\|\|\|\|\| \|\| \|\|\|\|\|@LB@ UGUGUCGU AGCAAC AG GAGUC@LB@3'-UUU...UGAUG UA AA U CCCUA...GCA-5'@LB@ \| \|@LB@ 70 43@LB@@LB@@LB@Energy -15.31 kcal/mol@LB@Hybridization Energy -25.5933 kcal/mol@LB@Unfolding Energy - mRNA 6.1914 kcal/mol@LB@Unfolding Energy - sRNA 4.09554 kcal/mol@LB@@LB@Position - mRNA 36 -- 59@LB@Position - sRNA 44 -- 69@LB@Position Seed - mRNA 37 -- 43@LB@Position Seed - sRNA 62 -- 68@LB@
126	0.00562	0.157	b3017	ftsP	-15.30	0.017476	190 -- 242	38 -- 84	septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)		_____________189____________________________________________________243\n_____________\|______________________________________________________\|\n5'-GAC...UUUAC____A_____U__GU___U____CGG____CAG________GGCAUC_______GCACU...CGC-3'\n______________AUGG_GCAAA_AU__CAC_CAGU____CGU___UUCAUUCA______GGGGAUU\n______________\|\|\|\|_\|\|\|\|\|_\|:__\|\|\|_\|\|\|:____\|\|\|___\|\|\|\|:\|\|\|______\|\|\|\|\|\|\|\n______________UACC_CGUUU_UG__GUG_GUCG____GCA___AAGUGAGU______CCCCUAA\n3'-UUU...CCCUC__________C__AU___U____UUAA___ACA_____________________AGGUA...GCA-5'\n_____________\|______________________________________________________\|\n_____________85_____________________________________________________37\n	236 -- 242	38 -- 44	-40.4092 kcal/mol	11.1124 kcal/mol	13.9994 kcal/mol	189 243@LB@ \| \|@LB@5'-GAC...UUUAC A U GU U CGG CAG GGCAUC GCACU...CGC-3'@LB@ AUGG GCAAA AU CAC CAGU CGU UUCAUUCA GGGGAUU@LB@ \|\|\|\| \|\|\|\|\| \|: \|\|\| \|\|\|: \|\|\| \|\|\|\|:\|\|\| \|\|\|\|\|\|\|@LB@ UACC CGUUU UG GUG GUCG GCA AAGUGAGU CCCCUAA@LB@3'-UUU...CCCUC C AU U UUAA ACA AGGUA...GCA-5'@LB@ \| \|@LB@ 85 37@LB@@LB@@LB@Energy -15.30 kcal/mol@LB@Hybridization Energy -40.4092 kcal/mol@LB@Unfolding Energy - mRNA 11.1124 kcal/mol@LB@Unfolding Energy - sRNA 13.9994 kcal/mol@LB@@LB@Position - mRNA 190 -- 242@LB@Position - sRNA 38 -- 84@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 38 -- 44@LB@
57	0.002011	0.1223	b3057	bacA	-16.67	0.007222	149 -- 193	41 -- 71	undecaprenyl pyrophosphate phosphatase		_____________148___________________________________________194\n_____________\|_____________________________________________\|\n5'-UUC...UGGUC_______________GACAAG_GGUACAUUUUAAUA_________UUUAU...UUG-3'\n______________AUACGCAGUAGUUCG______C______________AUUUAGGGG\n______________\|:\|\|:\|\|\|:\|:\|\|\|\|______\|______________\|:\|:\|\|\|\|\|\n______________UGUGUGUCGUUAAGC______G______________UGAGUCCCC\n3'-UUU...UCUGA_______________AACAAA________________________UAAAG...GCA-5'\n_____________\|_____________________________________________\|\n_____________72____________________________________________40\n	187 -- 193	41 -- 47	-30.087 kcal/mol	8.67993 kcal/mol	4.74226 kcal/mol	148 194@LB@ \| \|@LB@5'-UUC...UGGUC GACAAG GGUACAUUUUAAUA UUUAU...UUG-3'@LB@ AUACGCAGUAGUUCG C AUUUAGGGG@LB@ \|:\|\|:\|\|\|:\|:\|\|\|\| \| \|:\|:\|\|\|\|\|@LB@ UGUGUGUCGUUAAGC G UGAGUCCCC@LB@3'-UUU...UCUGA AACAAA UAAAG...GCA-5'@LB@ \| \|@LB@ 72 40@LB@@LB@@LB@Energy -16.67 kcal/mol@LB@Hybridization Energy -30.087 kcal/mol@LB@Unfolding Energy - mRNA 8.67993 kcal/mol@LB@Unfolding Energy - sRNA 4.74226 kcal/mol@LB@@LB@Position - mRNA 149 -- 193@LB@Position - sRNA 41 -- 71@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 41 -- 47@LB@
44	0.001243	0.09943	b3064	tsaD	-15.06	0.020167	98 -- 131	41 -- 82	tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein		_____________97___________________________________________132\n_____________\|____________________________________________\|\n5'-CAU...UAUAA_________G_______GA________________U________CGAAC...UGA-3'\n______________GGGUGAGGA_UAUACAC__GGC_________UUUC_C_CAGGGG\n______________\|\|\|::\|:\|\|_\|\|:\|\|\|\|__:\|\|_________\|\|\|\|_\|_\|\|\|\|\|\|\n______________CCCGUUUCU_AUGUGUG__UCG_________AAAG_G_GUCCCC\n3'-UUU...CUCUA_________G____________UUAAGCAAC____U_A______UAAAG...GCA-5'\n_____________\|____________________________________________\|\n_____________83___________________________________________40\n	108 -- 114	66 -- 72	-38.4936 kcal/mol	9.4641 kcal/mol	13.9685 kcal/mol	97 132@LB@ \| \|@LB@5'-CAU...UAUAA G GA U CGAAC...UGA-3'@LB@ GGGUGAGGA UAUACAC GGC UUUC C CAGGGG@LB@ \|\|\|::\|:\|\| \|\|:\|\|\|\| :\|\| \|\|\|\| \| \|\|\|\|\|\|@LB@ CCCGUUUCU AUGUGUG UCG AAAG G GUCCCC@LB@3'-UUU...CUCUA G UUAAGCAAC U A UAAAG...GCA-5'@LB@ \| \|@LB@ 83 40@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -38.4936 kcal/mol@LB@Unfolding Energy - mRNA 9.4641 kcal/mol@LB@Unfolding Energy - sRNA 13.9685 kcal/mol@LB@@LB@Position - mRNA 98 -- 131@LB@Position - sRNA 41 -- 82@LB@Position Seed - mRNA 108 -- 114@LB@Position Seed - sRNA 66 -- 72@LB@
88	0.003962	0.1538	b3084	rlmG	-11.78	0.115170	185 -- 197	40 -- 51	23S rRNA m(2)G1835 methyltransferase SAM-dependent		_____________184___________198\n_____________\|_____________\|\n5'-UGU...UUUUA_________A___CCGAU...AAU-3'\n______________UCAUUCAGG_GGG\n______________\|\|\|:\|\|\|\|\|_\|\|:\n______________AGUGAGUCC_CCU\n3'-UUU...AACAA_____________AAAGG...GCA-5'\n_____________\|_____________\|\n_____________52____________39\n	187 -- 193	43 -- 49	-16.9089 kcal/mol	3.37488 kcal/mol	1.76028 kcal/mol	184 198@LB@ \| \|@LB@5'-UGU...UUUUA A CCGAU...AAU-3'@LB@ UCAUUCAGG GGG@LB@ \|\|\|:\|\|\|\|\| \|\|:@LB@ AGUGAGUCC CCU@LB@3'-UUU...AACAA AAAGG...GCA-5'@LB@ \| \|@LB@ 52 39@LB@@LB@@LB@Energy -11.78 kcal/mol@LB@Hybridization Energy -16.9089 kcal/mol@LB@Unfolding Energy - mRNA 3.37488 kcal/mol@LB@Unfolding Energy - sRNA 1.76028 kcal/mol@LB@@LB@Position - mRNA 185 -- 197@LB@Position - sRNA 40 -- 51@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 43 -- 49@LB@
16	0.00012	0.02638	b3089	sstT	-15.87	0.012233	203 -- 224	42 -- 75	sodium:serine/threonine symporter		_____________202________________________________225\n_____________\|__________________________________\|\n5'-CAC...AAAAU_________ACG______________________CUAUU...CAU-3'\n______________GACUACGCA______________UUCAC_CGGGG\n______________\|\|\|\|\|\|:\|\|______________\|\|\|\|\|_\|:\|\|\|\n______________CUGAUGUGU______________AAGUG_GUCCC\n3'-UUU...CGUUU_________GUCGUUAAGCAACA_____A_____CUAAA...GCA-5'\n_____________\|__________________________________\|\n_____________76_________________________________41\n	205 -- 211	67 -- 73	-24.6329 kcal/mol	4.00159 kcal/mol	4.76397 kcal/mol	202 225@LB@ \| \|@LB@5'-CAC...AAAAU ACG CUAUU...CAU-3'@LB@ GACUACGCA UUCAC CGGGG@LB@ \|\|\|\|\|\|:\|\| \|\|\|\|\| \|:\|\|\|@LB@ CUGAUGUGU AAGUG GUCCC@LB@3'-UUU...CGUUU GUCGUUAAGCAACA A CUAAA...GCA-5'@LB@ \| \|@LB@ 76 41@LB@@LB@@LB@Energy -15.87 kcal/mol@LB@Hybridization Energy -24.6329 kcal/mol@LB@Unfolding Energy - mRNA 4.00159 kcal/mol@LB@Unfolding Energy - sRNA 4.76397 kcal/mol@LB@@LB@Position - mRNA 203 -- 224@LB@Position - sRNA 42 -- 75@LB@Position Seed - mRNA 205 -- 211@LB@Position Seed - sRNA 67 -- 73@LB@
185	0.009421	0.1776	b3094	exuR	-10.10	0.235203	51 -- 95	35 -- 75	hexuronate regulon transcriptional repressor; autorepressor		_____________50____________________________________________96\n_____________\|_____________________________________________\|\n5'-CCG...AUAAC___CC_____C____CAUUAAC_______GUA__UUUUA______CUUCA...CUG-3'\n______________GAU__CGCAC_GCAA_______GUUUCGU___AG_____GUUUCC\n______________\|\|:__\|:\|\|\|_\|\|\|\|_______\|\|\|\|\|::___\|\|_____:\|\|\|\|\|\n______________CUG__GUGUG_CGUU_______CAAAGUG___UC_____UAAAGG\n3'-UUU...CGUUU___AU_____U____AAGCAA________AG___CCC________UACGA...GCA-5'\n_____________\|_____________________________________________\|\n_____________76____________________________________________34\n	73 -- 79	48 -- 54	-21.0703 kcal/mol	6.16224 kcal/mol	4.80541 kcal/mol	50 96@LB@ \| \|@LB@5'-CCG...AUAAC CC C CAUUAAC GUA UUUUA CUUCA...CUG-3'@LB@ GAU CGCAC GCAA GUUUCGU AG GUUUCC@LB@ \|\|: \|:\|\|\| \|\|\|\| \|\|\|\|\|:: \|\| :\|\|\|\|\|@LB@ CUG GUGUG CGUU CAAAGUG UC UAAAGG@LB@3'-UUU...CGUUU AU U AAGCAA AG CCC UACGA...GCA-5'@LB@ \| \|@LB@ 76 34@LB@@LB@@LB@Energy -10.10 kcal/mol@LB@Hybridization Energy -21.0703 kcal/mol@LB@Unfolding Energy - mRNA 6.16224 kcal/mol@LB@Unfolding Energy - sRNA 4.80541 kcal/mol@LB@@LB@Position - mRNA 51 -- 95@LB@Position - sRNA 35 -- 75@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 48 -- 54@LB@
80	0.003376	0.1462	b3098	yqjD	-10.12	0.233597	155 -- 204	31 -- 75	membrane-anchored ribosome-binding protein		_____________154_________________________________________________205\n_____________\|___________________________________________________\|\n5'-UCA...CGCGC______UA__UCA____A___CACUAC_______CCU__A__AAA______CGAAA...AAG-3'\n______________GACUAC__AC___CAAU__GU______UUACUCA___GG_GA___CUAUGU\n______________\|\|\|\|\|\|__\|\|___\|\|\|\|__\|\|______\|:\|\|\|\|\|___\|\|_\|\|___\|:\|\|\|:\n______________CUGAUG__UG___GUUA__CA______AGUGAGU___CC_CU___GGUACG\n3'-UUU...CGUUU______UG__UC_____AG__ACAA_________C_______AAA______AAUAU...GCA-5'\n_____________\|___________________________________________________\|\n_____________76__________________________________________________30\n	181 -- 187	45 -- 51	-24.4421 kcal/mol	7.05998 kcal/mol	7.26183 kcal/mol	154 205@LB@ \| \|@LB@5'-UCA...CGCGC UA UCA A CACUAC CCU A AAA CGAAA...AAG-3'@LB@ GACUAC AC CAAU GU UUACUCA GG GA CUAUGU@LB@ \|\|\|\|\|\| \|\| \|\|\|\| \|\| \|:\|\|\|\|\| \|\| \|\| \|:\|\|\|:@LB@ CUGAUG UG GUUA CA AGUGAGU CC CU GGUACG@LB@3'-UUU...CGUUU UG UC AG ACAA C AAA AAUAU...GCA-5'@LB@ \| \|@LB@ 76 30@LB@@LB@@LB@Energy -10.12 kcal/mol@LB@Hybridization Energy -24.4421 kcal/mol@LB@Unfolding Energy - mRNA 7.05998 kcal/mol@LB@Unfolding Energy - sRNA 7.26183 kcal/mol@LB@@LB@Position - mRNA 155 -- 204@LB@Position - sRNA 31 -- 75@LB@Position Seed - mRNA 181 -- 187@LB@Position Seed - sRNA 45 -- 51@LB@
87	0.003844	0.1538	b3124	garK	-12.12	0.097757	2 -- 34	58 -- 88	glycerate kinase I		_____________1_________________________________35\n_____________\|_________________________________\|\n__________5'-U_______AU_____C______A____A______AGGAA...AUG-3'\n______________GGAGAUG__GCAGG_ACUGCG_GCAG_UGGUUU\n______________\|\|\|\|\|\|\|__\|\|\|::_\|\|\|:\|:_:\|\|\|_:::\|\|:\n______________CCUCUAC__CGUUU_UGAUGU_UGUC_GUUAAG\n3'-UUU...AGCAC_______C______C______G___________CAACA...GCA-5'\n_____________\|_________________________________\|\n_____________89________________________________57\n	2 -- 8	82 -- 88	-28.4892 kcal/mol	6.08629 kcal/mol	10.2803 kcal/mol	1 35@LB@ \| \|@LB@ 5'-U AU C A A AGGAA...AUG-3'@LB@ GGAGAUG GCAGG ACUGCG GCAG UGGUUU@LB@ \|\|\|\|\|\|\| \|\|\|:: \|\|\|:\|: :\|\|\| :::\|\|:@LB@ CCUCUAC CGUUU UGAUGU UGUC GUUAAG@LB@3'-UUU...AGCAC C C G CAACA...GCA-5'@LB@ \| \|@LB@ 89 57@LB@@LB@@LB@Energy -12.12 kcal/mol@LB@Hybridization Energy -28.4892 kcal/mol@LB@Unfolding Energy - mRNA 6.08629 kcal/mol@LB@Unfolding Energy - sRNA 10.2803 kcal/mol@LB@@LB@Position - mRNA 2 -- 34@LB@Position - sRNA 58 -- 88@LB@Position Seed - mRNA 2 -- 8@LB@Position Seed - sRNA 82 -- 88@LB@
122	0.005462	0.1553	b3143	yraI	-10.36	0.212908	69 -- 81	40 -- 51	putative periplasmic pilin chaperone	b0939 b4316 b0531	_____________68____________82\n_____________\|_____________\|\n5'-GGA...AGAAA____CC_______AGCAG...UGA-3'\n______________UCAC__CAGGGGA\n______________\|\|\|\|__\|\|\|\|\|\|\|\n______________AGUG__GUCCCCU\n3'-UUU...AACAA____A________AAAGG...GCA-5'\n_____________\|_____________\|\n_____________52____________39\n	75 -- 81	40 -- 46	-16.6795 kcal/mol	4.56539 kcal/mol	1.76028 kcal/mol	68 82@LB@ \| \|@LB@5'-GGA...AGAAA CC AGCAG...UGA-3'@LB@ UCAC CAGGGGA@LB@ \|\|\|\| \|\|\|\|\|\|\|@LB@ AGUG GUCCCCU@LB@3'-UUU...AACAA A AAAGG...GCA-5'@LB@ \| \|@LB@ 52 39@LB@@LB@@LB@Energy -10.36 kcal/mol@LB@Hybridization Energy -16.6795 kcal/mol@LB@Unfolding Energy - mRNA 4.56539 kcal/mol@LB@Unfolding Energy - sRNA 1.76028 kcal/mol@LB@@LB@Position - mRNA 69 -- 81@LB@Position - sRNA 40 -- 51@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 40 -- 46@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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