Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
132	0.005915	0.1577	b3356	yhfA	-11.83	0.112173	273 -- 293	39 -- 71	OsmC family protein	_____________272_______________________________294\n_____________\|_________________________________\|\n5'-CUG...UUUUA___G_________________________C___AAAGCAC-3'\n______________AUG_ACGGCA____________ACUCAGG_GAU\n______________\|::_\|\|:\|\|\|____________\|\|\|\|\|\|\|_\|\|\|\n______________UGU_UGUCGU____________UGAGUCC_CUA\n3'-UUU...UCUGA___G______UAAGCAACAAAG_______C___AAGGU...GCA-5'\n_____________\|_________________________________\|\n_____________72________________________________38\n	283 -- 289	43 -- 49	-20.9729 kcal/mol	4.393 kcal/mol	4.74947 kcal/mol	272 294@LB@ \| \|@LB@5'-CUG...UUUUA G C AAAGCAC-3'@LB@ AUG ACGGCA ACUCAGG GAU@LB@ \|:: \|\|:\|\|\| \|\|\|\|\|\|\| \|\|\|@LB@ UGU UGUCGU UGAGUCC CUA@LB@3'-UUU...UCUGA G UAAGCAACAAAG C AAGGU...GCA-5'@LB@ \| \|@LB@ 72 38@LB@@LB@@LB@Energy -11.83 kcal/mol@LB@Hybridization Energy -20.9729 kcal/mol@LB@Unfolding Energy - mRNA 4.393 kcal/mol@LB@Unfolding Energy - sRNA 4.74947 kcal/mol@LB@@LB@Position - mRNA 273 -- 293@LB@Position - sRNA 39 -- 71@LB@Position Seed - mRNA 283 -- 289@LB@Position Seed - sRNA 43 -- 49@LB@
30	0.0004324	0.05073	b3364	tsgA	-16.38	0.008733	203 -- 236	38 -- 75	putative transporter	_____________202_____________________________________237\n_____________\|_______________________________________\|\n5'-CCA...AUCAU___________________CAUCAA______CAU_____AGCUU...AUA-3'\n______________GACUA___ACAGCAAU_CG_______GCUCA___GGAUU\n______________\|\|\|\|\|___\|\|\|\|\|\|\|\|_\|\|_______:\|\|\|\|___\|\|\|\|\|\n______________CUGAU___UGUCGUUA_GC_______UGAGU___CCUAA\n3'-UUU...CGUUU_____GUG________A__AACAAAG_____CC______AGGUA...GCA-5'\n_____________\|_______________________________________\|\n_____________76______________________________________37\n	209 -- 215	60 -- 66	-26.4683 kcal/mol	5.30565 kcal/mol	4.77946 kcal/mol	202 237@LB@ \| \|@LB@5'-CCA...AUCAU CAUCAA CAU AGCUU...AUA-3'@LB@ GACUA ACAGCAAU CG GCUCA GGAUU@LB@ \|\|\|\|\| \|\|\|\|\|\|\|\| \|\| :\|\|\|\| \|\|\|\|\|@LB@ CUGAU UGUCGUUA GC UGAGU CCUAA@LB@3'-UUU...CGUUU GUG A AACAAAG CC AGGUA...GCA-5'@LB@ \| \|@LB@ 76 37@LB@@LB@@LB@Energy -16.38 kcal/mol@LB@Hybridization Energy -26.4683 kcal/mol@LB@Unfolding Energy - mRNA 5.30565 kcal/mol@LB@Unfolding Energy - sRNA 4.77946 kcal/mol@LB@@LB@Position - mRNA 203 -- 236@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 60 -- 66@LB@
125	0.005556	0.1564	b3384	trpS	-12.26	0.091693	207 -- 244	40 -- 100	tryptophanyl-tRNA synthetase	_____________206___________________________________________________________245\n_____________\|_____________________________________________________________\|\n5'-ACA...UGACU___________UUUUA__________________________________________U__AUUGA...AUG-3'\n______________AAGCCCAUCGU_____GUGG__________CGCACAGC____________C_CUCAGG_GA\n______________\|\|\|\|\|\|\|\|\|\|\|_____:\|\|\|__________\|:\|\|\|\|\|\|____________\|_\|\|\|\|\|\|_\|\|\n______________UUCGGGUAGCA_____UACC__________GUGUGUCG____________G_GAGUCC_CU\n______3'-UUUUU___________CCCUC____CGUUUCUGAU________UUAAGCAACAAA_U______C__AAAGG...GCA-5'\n_____________\|_____________________________________________________________\|\n_____________101___________________________________________________________39\n	228 -- 234	63 -- 69	-41.2838 kcal/mol	11.4457 kcal/mol	17.5818 kcal/mol	206 245@LB@ \| \|@LB@5'-ACA...UGACU UUUUA U AUUGA...AUG-3'@LB@ AAGCCCAUCGU GUGG CGCACAGC C CUCAGG GA@LB@ \|\|\|\|\|\|\|\|\|\|\| :\|\|\| \|:\|\|\|\|\|\| \| \|\|\|\|\|\| \|\|@LB@ UUCGGGUAGCA UACC GUGUGUCG G GAGUCC CU@LB@ 3'-UUUUU CCCUC CGUUUCUGAU UUAAGCAACAAA U C AAAGG...GCA-5'@LB@ \| \|@LB@ 101 39@LB@@LB@@LB@Energy -12.26 kcal/mol@LB@Hybridization Energy -41.2838 kcal/mol@LB@Unfolding Energy - mRNA 11.4457 kcal/mol@LB@Unfolding Energy - sRNA 17.5818 kcal/mol@LB@@LB@Position - mRNA 207 -- 244@LB@Position - sRNA 40 -- 100@LB@Position Seed - mRNA 228 -- 234@LB@Position Seed - sRNA 63 -- 69@LB@
37	0.0006599	0.0618	b3399	yrfG	-16.99	0.005777	176 -- 197	35 -- 56	GMP/IMP nucleotidase	_____________175____________________198\n_____________\|______________________\|\n5'-UAG...GCAAA___C_______U__A_______CUCAU...AGC-3'\n______________UUG_UUCACUC_GG_GAUUUCC\n______________\|\|\|_\|\|\|\|\|\|\|_\|\|_\|\|\|\|\|\|\|\n______________AAC_AAGUGAG_CC_CUAAAGG\n3'-UUU...UAAGC___A_______U__C_______UACGA...GCA-5'\n_____________\|______________________\|\n_____________57_____________________34\n	191 -- 197	35 -- 41	-24.095 kcal/mol	5.08619 kcal/mol	2.02101 kcal/mol	175 198@LB@ \| \|@LB@5'-UAG...GCAAA C U A CUCAU...AGC-3'@LB@ UUG UUCACUC GG GAUUUCC@LB@ \|\|\| \|\|\|\|\|\|\| \|\| \|\|\|\|\|\|\|@LB@ AAC AAGUGAG CC CUAAAGG@LB@3'-UUU...UAAGC A U C UACGA...GCA-5'@LB@ \| \|@LB@ 57 34@LB@@LB@@LB@Energy -16.99 kcal/mol@LB@Hybridization Energy -24.095 kcal/mol@LB@Unfolding Energy - mRNA 5.08619 kcal/mol@LB@Unfolding Energy - sRNA 2.02101 kcal/mol@LB@@LB@Position - mRNA 176 -- 197@LB@Position - sRNA 35 -- 56@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 35 -- 41@LB@
104	0.004609	0.1538	b3405	ompR	-12.19	0.094658	154 -- 190	42 -- 77	response regulator in two-component regulatory system with EnvZ	_____________153___________________________________191\n_____________\|_____________________________________\|\n5'-AAU...UUUUU____A_______UUA___A______CGAAC___U___AGUAC...GCG-3'\n______________AAGA_UACACGC___CAA_UUGUUG_____CUU_GGG\n______________\|\|\|\|_\|\|\|\|\|:\|___\|\|\|_\|:\|\|\|\|_____\|\|:_\|\|\|\n______________UUCU_AUGUGUG___GUU_AGCAAC_____GAG_CCC\n3'-UUU...CCCGU____G_______UC____A______AAAGU___U___CUAAA...GCA-5'\n_____________\|_____________________________________\|\n_____________78____________________________________41\n	159 -- 165	66 -- 72	-25.6517 kcal/mol	8.69679 kcal/mol	4.7648 kcal/mol	153 191@LB@ \| \|@LB@5'-AAU...UUUUU A UUA A CGAAC U AGUAC...GCG-3'@LB@ AAGA UACACGC CAA UUGUUG CUU GGG@LB@ \|\|\|\| \|\|\|\|\|:\| \|\|\| \|:\|\|\|\| \|\|: \|\|\|@LB@ UUCU AUGUGUG GUU AGCAAC GAG CCC@LB@3'-UUU...CCCGU G UC A AAAGU U CUAAA...GCA-5'@LB@ \| \|@LB@ 78 41@LB@@LB@@LB@Energy -12.19 kcal/mol@LB@Hybridization Energy -25.6517 kcal/mol@LB@Unfolding Energy - mRNA 8.69679 kcal/mol@LB@Unfolding Energy - sRNA 4.7648 kcal/mol@LB@@LB@Position - mRNA 154 -- 190@LB@Position - sRNA 42 -- 77@LB@Position Seed - mRNA 159 -- 165@LB@Position Seed - sRNA 66 -- 72@LB@
134	0.006141	0.1605	b3413	gntX	-10.72	0.183370	226 -- 267	37 -- 75	DNA catabolic protein	_____________225__________________________________________268\n_____________\|____________________________________________\|\n5'-CCC...AUGCU___________AAU__CACUGG____AGG_______________GUUCG...AUA-3'\n______________GGCUAUGC_CG___GC______CGUU___UCAUU_GGGGGAUUU\n______________\|:\|\|\|::\|_\|:___\|\|______\|\|\|\|___\|\|\|:\|_:\|\|\|\|\|\|\|\|\n______________CUGAUGUG_GU___CG______GCAA___AGUGA_UCCCCUAAA\n3'-UUU...CGUUU________U_______UUAA______CAA_____G_________GGUAC...GCA-5'\n_____________\|____________________________________________\|\n_____________76___________________________________________36\n	261 -- 267	37 -- 43	-30.3916 kcal/mol	14.8943 kcal/mol	4.77966 kcal/mol	225 268@LB@ \| \|@LB@5'-CCC...AUGCU AAU CACUGG AGG GUUCG...AUA-3'@LB@ GGCUAUGC CG GC CGUU UCAUU GGGGGAUUU@LB@ \|:\|\|\|::\| \|: \|\| \|\|\|\| \|\|\|:\| :\|\|\|\|\|\|\|\|@LB@ CUGAUGUG GU CG GCAA AGUGA UCCCCUAAA@LB@3'-UUU...CGUUU U UUAA CAA G GGUAC...GCA-5'@LB@ \| \|@LB@ 76 36@LB@@LB@@LB@Energy -10.72 kcal/mol@LB@Hybridization Energy -30.3916 kcal/mol@LB@Unfolding Energy - mRNA 14.8943 kcal/mol@LB@Unfolding Energy - sRNA 4.77966 kcal/mol@LB@@LB@Position - mRNA 226 -- 267@LB@Position - sRNA 37 -- 75@LB@Position Seed - mRNA 261 -- 267@LB@Position Seed - sRNA 37 -- 43@LB@
119	0.005328	0.1553	b3417	malP	-6.58	0.680720	220 -- 257	42 -- 71	maltodextrin phosphorylase	_____________219____________________________________258\n_____________\|______________________________________\|\n5'-ACA...UUUUA___AUA________CAG_AAGCGC______G____U__CAGCG...GCC-3'\n______________ACG___AGCAAUUU___G______UUUCAC_UCAG_GG\n______________\|\|:___\|\|\|\|\|\|\|:___\|______\|\|\|\|\|\|_\|\|\|\|_\|\|\n______________UGU___UCGUUAAG___C______AAAGUG_AGUC_CC\n3'-UUU...UCUGA___GUG________CAA_____________________CUAAA...GCA-5'\n_____________\|______________________________________\|\n_____________72_____________________________________41\n	227 -- 233	58 -- 64	-19.6486 kcal/mol	8.3321 kcal/mol	4.73391 kcal/mol	219 258@LB@ \| \|@LB@5'-ACA...UUUUA AUA CAG AAGCGC G U CAGCG...GCC-3'@LB@ ACG AGCAAUUU G UUUCAC UCAG GG@LB@ \|\|: \|\|\|\|\|\|\|: \| \|\|\|\|\|\| \|\|\|\| \|\|@LB@ UGU UCGUUAAG C AAAGUG AGUC CC@LB@3'-UUU...UCUGA GUG CAA CUAAA...GCA-5'@LB@ \| \|@LB@ 72 41@LB@@LB@@LB@Energy -6.58 kcal/mol@LB@Hybridization Energy -19.6486 kcal/mol@LB@Unfolding Energy - mRNA 8.3321 kcal/mol@LB@Unfolding Energy - sRNA 4.73391 kcal/mol@LB@@LB@Position - mRNA 220 -- 257@LB@Position - sRNA 42 -- 71@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 58 -- 64@LB@
67	0.002719	0.1417	b3454	livF	-11.67	0.120634	106 -- 122	40 -- 58	branched-chain amino acid ABC transporter ATPase	_____________105_________________123\n_____________\|___________________\|\n5'-AGA...AUUUA___GG_____A________CGCCG...UUG-3'\n______________CGU____UCA_UCAGGGGA\n______________\|\|\|____\|\|\|_\|\|\|\|\|\|\|\|\n______________GCA____AGU_AGUCCCCU\n3'-UUU...GUUAA___ACAA___G________AAAGG...GCA-5'\n_____________\|___________________\|\n_____________59__________________39\n	116 -- 122	40 -- 46	-19.8465 kcal/mol	5.75116 kcal/mol	2.42241 kcal/mol	105 123@LB@ \| \|@LB@5'-AGA...AUUUA GG A CGCCG...UUG-3'@LB@ CGU UCA UCAGGGGA@LB@ \|\|\| \|\|\| \|\|\|\|\|\|\|\|@LB@ GCA AGU AGUCCCCU@LB@3'-UUU...GUUAA ACAA G AAAGG...GCA-5'@LB@ \| \|@LB@ 59 39@LB@@LB@@LB@Energy -11.67 kcal/mol@LB@Hybridization Energy -19.8465 kcal/mol@LB@Unfolding Energy - mRNA 5.75116 kcal/mol@LB@Unfolding Energy - sRNA 2.42241 kcal/mol@LB@@LB@Position - mRNA 106 -- 122@LB@Position - sRNA 40 -- 58@LB@Position Seed - mRNA 116 -- 122@LB@Position Seed - sRNA 40 -- 46@LB@
10	6.564e-05	0.0231	b3458	livK	-17.14	0.005196	153 -- 196	35 -- 71	leucine transporter subunit	_____________152____________________________________________197\n_____________\|______________________________________________\|\n5'-UGU...UGUUA______AAAAACAA_____CACAACA______GAAU__________GACUA...CGA-3'\n______________ACACAU________AGCAA_________UCAC____GGGGAUUUUU\n______________\|\|\|\|\|:________\|\|\|\|\|_________\|\|\|\|____\|\|\|\|\|\|\|\|::\n______________UGUGUG________UCGUU_________AGUG____CCCCUAAAGG\n3'-UUU...UCUGA___________________AAGCAACAA____AGU___________UACGA...GCA-5'\n_____________\|______________________________________________\|\n_____________72_____________________________________________34\n	190 -- 196	35 -- 41	-26.3585 kcal/mol	4.43917 kcal/mol	4.78171 kcal/mol	152 197@LB@ \| \|@LB@5'-UGU...UGUUA AAAAACAA CACAACA GAAU GACUA...CGA-3'@LB@ ACACAU AGCAA UCAC GGGGAUUUUU@LB@ \|\|\|\|\|: \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|::@LB@ UGUGUG UCGUU AGUG CCCCUAAAGG@LB@3'-UUU...UCUGA AAGCAACAA AGU UACGA...GCA-5'@LB@ \| \|@LB@ 72 34@LB@@LB@@LB@Energy -17.14 kcal/mol@LB@Hybridization Energy -26.3585 kcal/mol@LB@Unfolding Energy - mRNA 4.43917 kcal/mol@LB@Unfolding Energy - sRNA 4.78171 kcal/mol@LB@@LB@Position - mRNA 153 -- 196@LB@Position - sRNA 35 -- 71@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 35 -- 41@LB@
24	0.0002076	0.02926	b3459	panM	-18.00	0.002751	9 -- 48	38 -- 75	PanD autocleavage accelerator panothenate synthesis	_____________8___________________________________________49\n_____________\|___________________________________________\|\n___5'-AAAAUAAC_______A_G___GGAAU_____ACUA________________AUGCU...UUG-3'\n______________GGC_ACA_A_GGC_____AAUUC______UCAUUCAGGGGAUU\n______________\|:\|_\|\|\|_\|_:\|\|_____\|\|\|\|\|______\|\|\|:\|\|\|\|\|\|\|\|\|\|\n______________CUG_UGU_U_UCG_____UUAAG______AGUGAGUCCCCUAA\n3'-UUU...CGUUU___A___G_G_____________CAACAA______________AGGUA...GCA-5'\n_____________\|___________________________________________\|\n_____________76__________________________________________37\n	42 -- 48	38 -- 44	-31.5918 kcal/mol	8.81239 kcal/mol	4.77946 kcal/mol	8 49@LB@ \| \|@LB@ 5'-AAAAUAAC A G GGAAU ACUA AUGCU...UUG-3'@LB@ GGC ACA A GGC AAUUC UCAUUCAGGGGAUU@LB@ \|:\| \|\|\| \| :\|\| \|\|\|\|\| \|\|\|:\|\|\|\|\|\|\|\|\|\|@LB@ CUG UGU U UCG UUAAG AGUGAGUCCCCUAA@LB@3'-UUU...CGUUU A G G CAACAA AGGUA...GCA-5'@LB@ \| \|@LB@ 76 37@LB@@LB@@LB@Energy -18.00 kcal/mol@LB@Hybridization Energy -31.5918 kcal/mol@LB@Unfolding Energy - mRNA 8.81239 kcal/mol@LB@Unfolding Energy - sRNA 4.77946 kcal/mol@LB@@LB@Position - mRNA 9 -- 48@LB@Position - sRNA 38 -- 75@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 38 -- 44@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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