Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
135	0.006184	0.1605	b0637	rsfS	-13.92	0.038847	179 -- 195	40 -- 56	ribosomal silencing factor	_____________178_______________196\n_____________\|_________________\|\n5'-AUU...UCUAU___A_____C_______AAAAC...UCA-3'\n______________UUG_UUCAC_CAGGGGG\n______________\|\|\|_\|\|\|\|\|_\|\|\|\|\|\|:\n______________AAC_AAGUG_GUCCCCU\n3'-UUU...UAAGC___A_____A_______AAAGG...GCA-5'\n_____________\|_________________\|\n_____________57________________39\n	189 -- 195	40 -- 46	-20.5369 kcal/mol	4.69018 kcal/mol	1.92548 kcal/mol	178 196@LB@ \| \|@LB@5'-AUU...UCUAU A C AAAAC...UCA-3'@LB@ UUG UUCAC CAGGGGG@LB@ \|\|\| \|\|\|\|\| \|\|\|\|\|\|:@LB@ AAC AAGUG GUCCCCU@LB@3'-UUU...UAAGC A A AAAGG...GCA-5'@LB@ \| \|@LB@ 57 39@LB@@LB@@LB@Energy -13.92 kcal/mol@LB@Hybridization Energy -20.5369 kcal/mol@LB@Unfolding Energy - mRNA 4.69018 kcal/mol@LB@Unfolding Energy - sRNA 1.92548 kcal/mol@LB@@LB@Position - mRNA 179 -- 195@LB@Position - sRNA 40 -- 56@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 40 -- 46@LB@
49	0.00161	0.1153	b0655	gltI	-14.54	0.027470	112 -- 169	31 -- 74	glutamate/aspartate periplasmic binding protein	_____________111________________________________________________________170\n_____________\|__________________________________________________________\|\n5'-AUU...ACAAC_______AAUAAAGUU____A____AACAACACAAA______CAAC____A_______GUUCU...UGG-3'\n______________ACUGCAC_________GCAG_CGAU___________CACUCA____GGGU_UCCAUGC\n______________\|\|\|:\|\|\|_________:\|\|\|_\|:\|\|___________\|\|\|\|\|\|____\|\|:\|_\|\|\|\|\|\|\|\n______________UGAUGUG_________UGUC_GUUA___________GUGAGU____CCUA_AGGUACG\n3'-UUU...GUUUC_________________________AGCAACAAA________CC______A_______AAUAU...GCA-5'\n_____________\|__________________________________________________________\|\n_____________75_________________________________________________________30\n	163 -- 169	31 -- 37	-33.5884 kcal/mol	11.7902 kcal/mol	7.26183 kcal/mol	111 170@LB@ \| \|@LB@5'-AUU...ACAAC AAUAAAGUU A AACAACACAAA CAAC A GUUCU...UGG-3'@LB@ ACUGCAC GCAG CGAU CACUCA GGGU UCCAUGC@LB@ \|\|\|:\|\|\| :\|\|\| \|:\|\| \|\|\|\|\|\| \|\|:\| \|\|\|\|\|\|\|@LB@ UGAUGUG UGUC GUUA GUGAGU CCUA AGGUACG@LB@3'-UUU...GUUUC AGCAACAAA CC A AAUAU...GCA-5'@LB@ \| \|@LB@ 75 30@LB@@LB@@LB@Energy -14.54 kcal/mol@LB@Hybridization Energy -33.5884 kcal/mol@LB@Unfolding Energy - mRNA 11.7902 kcal/mol@LB@Unfolding Energy - sRNA 7.26183 kcal/mol@LB@@LB@Position - mRNA 112 -- 169@LB@Position - sRNA 31 -- 74@LB@Position Seed - mRNA 163 -- 169@LB@Position Seed - sRNA 31 -- 37@LB@
175	0.008768	0.1747	b0658	ybeX	-12.37	0.086971	210 -- 256	35 -- 75	putative ion transport	_____________209_____________________________________________257\n_____________\|_______________________________________________\|\n5'-UUA...GCGAC___AAUU___________GACACGAUAAG__ACA__AA_________CUCCC...ACC-3'\n______________GAC____CACACAGUAGU___________CA___AG__GGGAUUUUU\n______________\|\|\|____\|\|\|\|\|\|\|:\|:\|___________\|\|___\|\|__\|\|\|\|\|\|\|::\n______________CUG____GUGUGUCGUUA___________GU___UC__CCCUAAAGG\n3'-UUU...CGUUU___AU_____________AGCAACAAA____GAG_____________UACGA...GCA-5'\n_____________\|_______________________________________________\|\n_____________76______________________________________________34\n	249 -- 255	36 -- 42	-26.9454 kcal/mol	9.77444 kcal/mol	4.80541 kcal/mol	209 257@LB@ \| \|@LB@5'-UUA...GCGAC AAUU GACACGAUAAG ACA AA CUCCC...ACC-3'@LB@ GAC CACACAGUAGU CA AG GGGAUUUUU@LB@ \|\|\| \|\|\|\|\|\|\|:\|:\| \|\| \|\| \|\|\|\|\|\|\|::@LB@ CUG GUGUGUCGUUA GU UC CCCUAAAGG@LB@3'-UUU...CGUUU AU AGCAACAAA GAG UACGA...GCA-5'@LB@ \| \|@LB@ 76 34@LB@@LB@@LB@Energy -12.37 kcal/mol@LB@Hybridization Energy -26.9454 kcal/mol@LB@Unfolding Energy - mRNA 9.77444 kcal/mol@LB@Unfolding Energy - sRNA 4.80541 kcal/mol@LB@@LB@Position - mRNA 210 -- 256@LB@Position - sRNA 35 -- 75@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 36 -- 42@LB@
1	5.015e-10	1.765e-06	b0680	glnS	-23.71	0.000006	155 -- 198	34 -- 77	glutamyl-tRNA synthetase	_____________154___________________________________________199\n_____________\|_____________________________________________\|\n5'-UGC...CUUAU_____C______C__U__A________A_____U_A___A_____CGAUG...CGC-3'\n______________AAGAU_AUACGC_GU_AU_CGUUGUUU_CGCUU_G_GGA__UCCA\n______________\|\|\|\|:_\|:\|\|:\|_\|:_\|\|_\|\|\|\|\|\|\|\|_\|:\|\|:_\|_\|\|\|__\|\|\|\|\n______________UUCUG_UGUGUG_CG_UA_GCAACAAA_GUGAG_C_CCU__AGGU\n3'-UUU...CCCGU_____A______U__U__A______________U_C___AA____ACGAA...GCA-5'\n_____________\|_____________________________________________\|\n_____________78____________________________________________33\n	175 -- 181	51 -- 57	-34.8912 kcal/mol	6.37021 kcal/mol	4.81601 kcal/mol	154 199@LB@ \| \|@LB@5'-UGC...CUUAU C C U A A U A A CGAUG...CGC-3'@LB@ AAGAU AUACGC GU AU CGUUGUUU CGCUU G GGA UCCA@LB@ \|\|\|\|: \|:\|\|:\| \|: \|\| \|\|\|\|\|\|\|\| \|:\|\|: \| \|\|\| \|\|\|\|@LB@ UUCUG UGUGUG CG UA GCAACAAA GUGAG C CCU AGGU@LB@3'-UUU...CCCGU A U U A U C AA ACGAA...GCA-5'@LB@ \| \|@LB@ 78 33@LB@@LB@@LB@Energy -23.71 kcal/mol@LB@Hybridization Energy -34.8912 kcal/mol@LB@Unfolding Energy - mRNA 6.37021 kcal/mol@LB@Unfolding Energy - sRNA 4.81601 kcal/mol@LB@@LB@Position - mRNA 155 -- 198@LB@Position - sRNA 34 -- 77@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 51 -- 57@LB@
129	0.005822	0.1577	b0713	ybgL	-14.99	0.021088	226 -- 247	61 -- 83	UPF0271 family protein	_____________225_____________________248\n_____________\|_______________________\|\n5'-GCC...CGAUC_______________________CGCAG...AUG-3'\n______________UGGGCGAAGGCUGCGC_CAGCGA\n______________\|\|\|\|\|:\|\|\|:\|\|:\|:\|_\|\|\|\|:\|\n______________ACCCGUUUCUGAUGUG_GUCGUU\n3'-UUU...CCUCU________________U______AAGCA...GCA-5'\n_____________\|_______________________\|\n_____________84______________________60\n	234 -- 240	69 -- 75	-36.9571 kcal/mol	11.7384 kcal/mol	10.2322 kcal/mol	225 248@LB@ \| \|@LB@5'-GCC...CGAUC CGCAG...AUG-3'@LB@ UGGGCGAAGGCUGCGC CAGCGA@LB@ \|\|\|\|\|:\|\|\|:\|\|:\|:\| \|\|\|\|:\|@LB@ ACCCGUUUCUGAUGUG GUCGUU@LB@3'-UUU...CCUCU U AAGCA...GCA-5'@LB@ \| \|@LB@ 84 60@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -36.9571 kcal/mol@LB@Unfolding Energy - mRNA 11.7384 kcal/mol@LB@Unfolding Energy - sRNA 10.2322 kcal/mol@LB@@LB@Position - mRNA 226 -- 247@LB@Position - sRNA 61 -- 83@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 69 -- 75@LB@
15	0.0001044	0.0245	b0741	pal	-16.61	0.007509	30 -- 75	30 -- 71	peptidoglycan-associated outer membrane lipoprotein	_____________29_____________________________________________76\n_____________\|______________________________________________\|\n5'-GGA...UAACG____U________CUACAGCUCUU__________GGGA________GAAUU...GCG-3'\n______________GCAC_AUGGUAAU___________CUCAGGGGAU____UCCGUGCU\n______________:\|\|\|_\|::\|:\|\|\|___________\|\|\|\|\|\|\|\|\|\|____\|\|\|:\|\|\|\|\n______________UGUG_UGUCGUUA___________GAGUCCCCUA____AGGUACGA\n3'-UUU...UCUGA_____________AGCAACAAAGU__________A___________AUAUU...GCA-5'\n_____________\|______________________________________________\|\n_____________72_____________________________________________29\n	69 -- 75	30 -- 36	-38.4662 kcal/mol	13.7923 kcal/mol	8.066 kcal/mol	29 76@LB@ \| \|@LB@5'-GGA...UAACG U CUACAGCUCUU GGGA GAAUU...GCG-3'@LB@ GCAC AUGGUAAU CUCAGGGGAU UCCGUGCU@LB@ :\|\|\| \|::\|:\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|:\|\|\|\|@LB@ UGUG UGUCGUUA GAGUCCCCUA AGGUACGA@LB@3'-UUU...UCUGA AGCAACAAAGU A AUAUU...GCA-5'@LB@ \| \|@LB@ 72 29@LB@@LB@@LB@Energy -16.61 kcal/mol@LB@Hybridization Energy -38.4662 kcal/mol@LB@Unfolding Energy - mRNA 13.7923 kcal/mol@LB@Unfolding Energy - sRNA 8.066 kcal/mol@LB@@LB@Position - mRNA 30 -- 75@LB@Position - sRNA 30 -- 71@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 30 -- 36@LB@
193	0.0098	0.1776	b0757	galK	-12.45	0.083592	134 -- 176	33 -- 76	galactokinase	_____________133____________________________________________177\n_____________\|______________________________________________\|\n5'-CAU...ACCGC________G_______AG__U___CGCG__G____C____A_____UUUCG...GUG-3'\n______________AGA__ACA_GCAGCAG__CG_UUG____CA_UCAG__GAU_UCCAU\n______________\|\|\|__\|\|\|_:\|\|\|\|\|:__\|\|_\|\|\|____\|\|_\|\|\|\|__\|\|\|_\|\|\|\|\|\n______________UCU__UGU_UGUCGUU__GC_AAC____GU_AGUC__CUA_AGGUA\n3'-UUU...CCGUU___GA___G_______AA______AAA___G____CC___A_____CGAAU...GCA-5'\n_____________\|______________________________________________\|\n_____________77_____________________________________________32\n	141 -- 147	61 -- 67	-31.8657 kcal/mol	14.6029 kcal/mol	4.81602 kcal/mol	133 177@LB@ \| \|@LB@5'-CAU...ACCGC G AG U CGCG G C A UUUCG...GUG-3'@LB@ AGA ACA GCAGCAG CG UUG CA UCAG GAU UCCAU@LB@ \|\|\| \|\|\| :\|\|\|\|\|: \|\| \|\|\| \|\| \|\|\|\| \|\|\| \|\|\|\|\|@LB@ UCU UGU UGUCGUU GC AAC GU AGUC CUA AGGUA@LB@3'-UUU...CCGUU GA G AA AAA G CC A CGAAU...GCA-5'@LB@ \| \|@LB@ 77 32@LB@@LB@@LB@Energy -12.45 kcal/mol@LB@Hybridization Energy -31.8657 kcal/mol@LB@Unfolding Energy - mRNA 14.6029 kcal/mol@LB@Unfolding Energy - sRNA 4.81602 kcal/mol@LB@@LB@Position - mRNA 134 -- 176@LB@Position - sRNA 33 -- 76@LB@Position Seed - mRNA 141 -- 147@LB@Position Seed - sRNA 61 -- 67@LB@
86	0.003687	0.1509	b0774	bioA	-10.95	0.166636	92 -- 152	44 -- 98	7 8-diaminopelargonic acid synthase PLP-dependent	_____________91_____________________________________________________________153\n_____________\|______________________________________________________________\|\n5'-ACG...ACAAU_____G______C____A__CAU______UCUCCAAA___U__UU_________A_______UGUAG...AAG-3'\n______________GUCCA_CGUGGG_GGUG_GC___GGGGCU________ACG_GU__UUUGUUGUU__AUUCGG\n______________\|:\|\|\|_\|\|\|\|\|\|_\|:\|\|_\|\|___::\|:\|\|________\|\|:_\|:__\|\|:\|\|\|\|\|\|__\|:\|\|:\|\n______________CGGGU_GCACCC_CUAC_CG___UUCUGA________UGU_CG__AAGCAACAA__UGAGUC\n____3'-UUUUUUU_____A______U____C__U________UGUG__________UU_________AG______CCCUA...GCA-5'\n_____________\|______________________________________________________________\|\n_____________99_____________________________________________________________43\n	139 -- 145	52 -- 58	-48.0349 kcal/mol	20.1482 kcal/mol	16.9429 kcal/mol	91 153@LB@ \| \|@LB@5'-ACG...ACAAU G C A CAU UCUCCAAA U UU A UGUAG...AAG-3'@LB@ GUCCA CGUGGG GGUG GC GGGGCU ACG GU UUUGUUGUU AUUCGG@LB@ \|:\|\|\| \|\|\|\|\|\| \|:\|\| \|\| ::\|:\|\| \|\|: \|: \|\|:\|\|\|\|\|\| \|:\|\|:\|@LB@ CGGGU GCACCC CUAC CG UUCUGA UGU CG AAGCAACAA UGAGUC@LB@ 3'-UUUUUUU A U C U UGUG UU AG CCCUA...GCA-5'@LB@ \| \|@LB@ 99 43@LB@@LB@@LB@Energy -10.95 kcal/mol@LB@Hybridization Energy -48.0349 kcal/mol@LB@Unfolding Energy - mRNA 20.1482 kcal/mol@LB@Unfolding Energy - sRNA 16.9429 kcal/mol@LB@@LB@Position - mRNA 92 -- 152@LB@Position - sRNA 44 -- 98@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 52 -- 58@LB@
85	0.003677	0.1509	b0813	rhtA	-9.71	0.273689	106 -- 123	34 -- 46	threonine and homoserine efflux system	_____________105________________124\n_____________\|__________________\|\n5'-CAG...CAAAA_______AAAUU______ACAAA...CAC-3'\n______________CGGGGGA_____UUUUCA\n______________\|:\|\|\|\|\|_____\|\|\|:\|\|\n______________GUCCCCU_____AAAGGU\n3'-UUU...AGUGA__________________ACGAA...GCA-5'\n_____________\|__________________\|\n_____________47_________________33\n	106 -- 112	40 -- 46	-14.6812 kcal/mol	3.4305 kcal/mol	1.54427 kcal/mol	105 124@LB@ \| \|@LB@5'-CAG...CAAAA AAAUU ACAAA...CAC-3'@LB@ CGGGGGA UUUUCA@LB@ \|:\|\|\|\|\| \|\|\|:\|\|@LB@ GUCCCCU AAAGGU@LB@3'-UUU...AGUGA ACGAA...GCA-5'@LB@ \| \|@LB@ 47 33@LB@@LB@@LB@Energy -9.71 kcal/mol@LB@Hybridization Energy -14.6812 kcal/mol@LB@Unfolding Energy - mRNA 3.4305 kcal/mol@LB@Unfolding Energy - sRNA 1.54427 kcal/mol@LB@@LB@Position - mRNA 106 -- 123@LB@Position - sRNA 34 -- 46@LB@Position Seed - mRNA 106 -- 112@LB@Position Seed - sRNA 40 -- 46@LB@
84	0.003656	0.1509	b0827	moeA	-7.84	0.503176	157 -- 203	32 -- 75	molybdopterin molybdenumtransferase; molybdopterin biosynthesis protein	_____________156_______________________________________________204\n_____________\|_________________________________________________\|\n5'-AUU...UUUCU_______AUA___A______AUU___CC____G____A_U_________GAAUU...UAC-3'\n______________GAC_AUA___GGC_AAUUCG___UUG__UC_C_CAGG_G_GUUUUCAUG\n______________\|\|\|_\|:\|___:\|\|_\|\|\|\|\|\|___\|\|\|__\|\|_\|_\|\|\|\|_\|_:\|\|\|:\|\|\|\|\n______________CUG_UGU___UCG_UUAAGC___AAC__AG_G_GUCC_C_UAAAGGUAC\n3'-UUU...CGUUU___A___GUG________________AA__U_A______C_________GAAUA...GCA-5'\n_____________\|_________________________________________________\|\n_____________76________________________________________________31\n	197 -- 203	32 -- 38	-28.9008 kcal/mol	13.8106 kcal/mol	7.24967 kcal/mol	156 204@LB@ \| \|@LB@5'-AUU...UUUCU AUA A AUU CC G A U GAAUU...UAC-3'@LB@ GAC AUA GGC AAUUCG UUG UC C CAGG G GUUUUCAUG@LB@ \|\|\| \|:\| :\|\| \|\|\|\|\|\| \|\|\| \|\| \| \|\|\|\| \| :\|\|\|:\|\|\|\|@LB@ CUG UGU UCG UUAAGC AAC AG G GUCC C UAAAGGUAC@LB@3'-UUU...CGUUU A GUG AA U A C GAAUA...GCA-5'@LB@ \| \|@LB@ 76 31@LB@@LB@@LB@Energy -7.84 kcal/mol@LB@Hybridization Energy -28.9008 kcal/mol@LB@Unfolding Energy - mRNA 13.8106 kcal/mol@LB@Unfolding Energy - sRNA 7.24967 kcal/mol@LB@@LB@Position - mRNA 157 -- 203@LB@Position - sRNA 32 -- 75@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 32 -- 38@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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