Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
117	0.01855	0.5155	b3011	yqhD	-12.43	0.029448	82 -- 109	34 -- 64	aldehyde reductase NADPH-dependent	_____________81________________________________110\n_____________\|_________________________________\|\n5'-GUU...CUGGA________CA____________GG_________GCAUU...UGA-3'\n______________GAAAUAGG_______AGACAUU__CAGAAAUGA\n______________\|\|\|\|\|\|\|\|_______\|\|\|\|\|\|\|__\|\|\|\|\|\|\|\|\|\n______________CUUUAUCC_______UCUGUAA__GUCUUUACU\n3'-UUU...GCCAA________CCAUUUG__________________UACUG...UCG-5'\n_____________\|_________________________________\|\n_____________65________________________________33\n	83 -- 89	57 -- 63	-24.9555 kcal/mol	6.2587 kcal/mol	6.27071 kcal/mol	81 110@LB@ \| \|@LB@5'-GUU...CUGGA CA GG GCAUU...UGA-3'@LB@ GAAAUAGG AGACAUU CAGAAAUGA@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ CUUUAUCC UCUGUAA GUCUUUACU@LB@3'-UUU...GCCAA CCAUUUG UACUG...UCG-5'@LB@ \| \|@LB@ 65 33@LB@@LB@@LB@Energy -12.43 kcal/mol@LB@Hybridization Energy -24.9555 kcal/mol@LB@Unfolding Energy - mRNA 6.2587 kcal/mol@LB@Unfolding Energy - sRNA 6.27071 kcal/mol@LB@@LB@Position - mRNA 82 -- 109@LB@Position - sRNA 34 -- 64@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 57 -- 63@LB@
67	0.009794	0.4523	b2823	ppdC	-12.40	0.029910	245 -- 275	3 -- 28	putative prepilin peptidase-dependent protein	_____________244_____________________________276\n_____________\|_______________________________\|\n5'-ACG...CCGGA_______UUGGC_____U_____________AUUGU...UCC-3'\n______________GGUAAUG_____GAUGG_GUUGAUGGUGAUG\n______________\|\|\|\|\|\|:_____:\|\|::_\|\|\|:\|\|\|:\|\|\|\|:\n______________CCAUUAU_____UUAUU_CAAUUACUACUAU\n3'-UUU...UACUG_________________U_____________CG-5'\n_____________\|_______________________________\|\n_____________29______________________________2\n	269 -- 275	3 -- 9	-21.5159 kcal/mol	8.35299 kcal/mol	0.766561 kcal/mol	244 276@LB@ \| \|@LB@5'-ACG...CCGGA UUGGC U AUUGU...UCC-3'@LB@ GGUAAUG GAUGG GUUGAUGGUGAUG@LB@ \|\|\|\|\|\|: :\|\|:: \|\|\|:\|\|\|:\|\|\|\|:@LB@ CCAUUAU UUAUU CAAUUACUACUAU@LB@3'-UUU...UACUG U CG-5'@LB@ \| \|@LB@ 29 2@LB@@LB@@LB@Energy -12.40 kcal/mol@LB@Hybridization Energy -21.5159 kcal/mol@LB@Unfolding Energy - mRNA 8.35299 kcal/mol@LB@Unfolding Energy - sRNA 0.766561 kcal/mol@LB@@LB@Position - mRNA 245 -- 275@LB@Position - sRNA 3 -- 28@LB@Position Seed - mRNA 269 -- 275@LB@Position Seed - sRNA 3 -- 9@LB@
57	0.007764	0.4472	b0585	fes	-12.40	0.029926	182 -- 237	1 -- 55	enterobactin/ferrienterobactin esterase	_____________181___________________________________________________________238\n_____________\|_____________________________________________________________\|\n5'-CAU...GCACA_______U__G____________U_______CGU____AGU__GA_____GAGC_______AGUCG...GGU-3'\n______________GUAAACA_GG_GUU_____AUGG_GUGACGG___UAAA___AG__AGUGA____UGGUGGC\n______________\|\|\|\|\|\|\|_\|:_:\|\|_____\|\|\|:_:\|\|\|\|\|\|___\|\|\|\|___\|\|__\|:\|\|\|____\|\|:\|:\|\|\n______________CAUUUGU_CU_UAA_____UACU_UACUGCC___AUUU___UC__UUACU____ACUAUCG\n3'-UUU...AUCCC__________G___GUCUU____________AUU____AUU__AA________________-5'\n_____________\|____________________________________________________________\|\n_____________56___________________________________________________________1\n	231 -- 237	1 -- 7	-34.1879 kcal/mol	15.4551 kcal/mol	6.33758 kcal/mol	181 238@LB@ \| \|@LB@5'-CAU...GCACA U G U CGU AGU GA GAGC AGUCG...GGU-3'@LB@ GUAAACA GG GUU AUGG GUGACGG UAAA AG AGUGA UGGUGGC@LB@ \|\|\|\|\|\|\| \|: :\|\| \|\|\|: :\|\|\|\|\|\| \|\|\|\| \|\| \|:\|\|\| \|\|:\|:\|\|@LB@ CAUUUGU CU UAA UACU UACUGCC AUUU UC UUACU ACUAUCG@LB@3'-UUU...AUCCC G GUCUU AUU AUU AA -5'@LB@ \| \|@LB@ 56 1@LB@@LB@@LB@Energy -12.40 kcal/mol@LB@Hybridization Energy -34.1879 kcal/mol@LB@Unfolding Energy - mRNA 15.4551 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 182 -- 237@LB@Position - sRNA 1 -- 55@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 1 -- 7@LB@
69	0.009861	0.4523	b0879	macB	-12.35	0.030572	144 -- 182	1 -- 59	macrolide ABC transporter peremase/ATPase	_____________143___________________________________________________________183\n_____________\|_____________________________________________________________\|\n5'-AAU...GUCAA____CUUG_____________________________________________________CAAAC...CGG-3'\n______________AGGG____AAGCGGGC_______GAUGAA_G_UGGUGAU____________UGGUGA_GGC\n______________\|\|\|\|____\|\|:\|:\|:\|_______:\|\|\|\|\|_\|_:\|\|\|:\|\|____________\|\|:\|\|\|_:\|\|\n______________UCCC____UUUGUCUG_______UUACUU_C_GCCAUUA____________ACUACU_UCG\n3'-UUU...CUUUA____CA__________UAAGUCU______A_U_______UUUAUUUCAAUU______A___-5'\n_____________\|____________________________________________________________\|\n_____________60___________________________________________________________1\n	167 -- 173	23 -- 29	-29.0987 kcal/mol	10.4067 kcal/mol	6.33764 kcal/mol	143 183@LB@ \| \|@LB@5'-AAU...GUCAA CUUG CAAAC...CGG-3'@LB@ AGGG AAGCGGGC GAUGAA G UGGUGAU UGGUGA GGC@LB@ \|\|\|\| \|\|:\|:\|:\| :\|\|\|\|\| \| :\|\|\|:\|\| \|\|:\|\|\| :\|\|@LB@ UCCC UUUGUCUG UUACUU C GCCAUUA ACUACU UCG@LB@3'-UUU...CUUUA CA UAAGUCU A U UUUAUUUCAAUU A -5'@LB@ \| \|@LB@ 60 1@LB@@LB@@LB@Energy -12.35 kcal/mol@LB@Hybridization Energy -29.0987 kcal/mol@LB@Unfolding Energy - mRNA 10.4067 kcal/mol@LB@Unfolding Energy - sRNA 6.33764 kcal/mol@LB@@LB@Position - mRNA 144 -- 182@LB@Position - sRNA 1 -- 59@LB@Position Seed - mRNA 167 -- 173@LB@Position Seed - sRNA 23 -- 29@LB@
81	0.01129	0.4523	b1718	infC	-12.35	0.030679	105 -- 150	1 -- 56	translation initiation factor IF-3	_____________104______________________________________________________151\n_____________\|________________________________________________________\|\n5'-UUG...GCCGU___________C________GCAUG__________________GAUC_________UGCAA...AGC-3'\n______________GGUAA__AGAC___UGGGAA_____GACG____UAAAUGAAGU____GA_GA_AGC\n______________\|\|\|\|\|__\|\|\|\|___::\|:\|\|_____\|\|\|\|____\|\|\|\|\|:\|\|\|\|____\|\|_\|\|_\|\|\|\n______________CCAUU__UCUG___GUCUUU_____CUGC____AUUUAUUUCA____CU_CU_UCG\n3'-UUU...UAUCC_____UG____UAA______ACUUA____CAUU__________AUUA__A__A___-5'\n_____________\|_______________________________________________________\|\n_____________57______________________________________________________1\n	133 -- 139	14 -- 20	-27.0853 kcal/mol	8.40012 kcal/mol	6.33758 kcal/mol	104 151@LB@ \| \|@LB@5'-UUG...GCCGU C GCAUG GAUC UGCAA...AGC-3'@LB@ GGUAA AGAC UGGGAA GACG UAAAUGAAGU GA GA AGC@LB@ \|\|\|\|\| \|\|\|\| ::\|:\|\| \|\|\|\| \|\|\|\|\|:\|\|\|\| \|\| \|\| \|\|\|@LB@ CCAUU UCUG GUCUUU CUGC AUUUAUUUCA CU CU UCG@LB@3'-UUU...UAUCC UG UAA ACUUA CAUU AUUA A A -5'@LB@ \| \|@LB@ 57 1@LB@@LB@@LB@Energy -12.35 kcal/mol@LB@Hybridization Energy -27.0853 kcal/mol@LB@Unfolding Energy - mRNA 8.40012 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 105 -- 150@LB@Position - sRNA 1 -- 56@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 14 -- 20@LB@
42	0.004307	0.3367	b4051	qorA	-12.29	0.031673	118 -- 140	3 -- 26	quinone oxidoreductase NADPH-dependent	_____________117______________________141\n_____________\|________________________\|\n5'-CGC...UGCCA_______________CC_______AAGAA...UCG-3'\n______________UAGUGAAUGGAGUUA__GA_GAUA\n______________\|\|:\|:\|\|\|::\|\|\|\|\|__\|\|_\|\|\|\|\n______________AUUAUUUAUUUCAAU__CU_CUAU\n3'-UUU...CUGCC_______________UA__A____CG-5'\n_____________\|________________________\|\n_____________27_______________________2\n	126 -- 132	12 -- 18	-16.4059 kcal/mol	3.53962 kcal/mol	0.580109 kcal/mol	117 141@LB@ \| \|@LB@5'-CGC...UGCCA CC AAGAA...UCG-3'@LB@ UAGUGAAUGGAGUUA GA GAUA@LB@ \|\|:\|:\|\|\|::\|\|\|\|\| \|\| \|\|\|\|@LB@ AUUAUUUAUUUCAAU CU CUAU@LB@3'-UUU...CUGCC UA A CG-5'@LB@ \| \|@LB@ 27 2@LB@@LB@@LB@Energy -12.29 kcal/mol@LB@Hybridization Energy -16.4059 kcal/mol@LB@Unfolding Energy - mRNA 3.53962 kcal/mol@LB@Unfolding Energy - sRNA 0.580109 kcal/mol@LB@@LB@Position - mRNA 118 -- 140@LB@Position - sRNA 3 -- 26@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 12 -- 18@LB@
89	0.01433	0.5059	b0636	rlmH	-12.18	0.033475	189 -- 207	1 -- 19	23S rRNA m(3)Psi1915 pseudouridine methyltransferase SAM-dependent	_____________188__________________208\n_____________\|____________________\|\n5'-CGG...AACUC___________C________UGCAA...UCG-3'\n______________UGGAGUUAAUG_GUGA_AGC\n______________\|::\|\|\|\|\|\|\|\|_:\|\|\|_\|\|\|\n______________AUUUCAAUUAC_UACU_UCG\n3'-UUU...UAUUU________________A___-5'\n_____________\|___________________\|\n_____________20__________________1\n	193 -- 199	9 -- 15	-16.4949 kcal/mol	4.25175 kcal/mol	0.0638423 kcal/mol	188 208@LB@ \| \|@LB@5'-CGG...AACUC C UGCAA...UCG-3'@LB@ UGGAGUUAAUG GUGA AGC@LB@ \|::\|\|\|\|\|\|\|\| :\|\|\| \|\|\|@LB@ AUUUCAAUUAC UACU UCG@LB@3'-UUU...UAUUU A -5'@LB@ \| \|@LB@ 20 1@LB@@LB@@LB@Energy -12.18 kcal/mol@LB@Hybridization Energy -16.4949 kcal/mol@LB@Unfolding Energy - mRNA 4.25175 kcal/mol@LB@Unfolding Energy - sRNA 0.0638423 kcal/mol@LB@@LB@Position - mRNA 189 -- 207@LB@Position - sRNA 1 -- 19@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 9 -- 15@LB@
55	0.006796	0.4056	b3192	mlaC	-12.17	0.033659	167 -- 226	1 -- 57	ABC transporter maintaining OM lipid asymmetry periplasmic binding protein	_____________166_______________________________________________________________227\n_____________\|_________________________________________________________________\|\n5'-GGA...CCUGU_____C______AAUAAU____________CC____C_____UU____C_U___________C__UUUGC...AGG-3'\n______________GGGUA_AAC_GA______UUCAGGA__GAA__GACG___AUG__UAAA_G_UUAAUGAUGGU_GC\n______________\|\|\|\|\|_\|\|\|_\|\|______\|\|\|\|\|:\|__\|\|\|__\|\|\|\|___\|\|:__\|\|\|\|_\|_\|\|\|\|\|\|\|\|\|:\|_\|\|\n______________CCCAU_UUG_CU______AAGUCUU__CUU__CUGC___UAU__AUUU_C_AAUUACUACUA_CG\n3'-UUU...UUAUC_________U__GU___________UA___A_____CAU___UU__________________U__-5'\n_____________\|________________________________________________________________\|\n_____________58_______________________________________________________________1\n	217 -- 223	4 -- 10	-32.157 kcal/mol	13.6506 kcal/mol	6.33758 kcal/mol	166 227@LB@ \| \|@LB@5'-GGA...CCUGU C AAUAAU CC C UU C U C UUUGC...AGG-3'@LB@ GGGUA AAC GA UUCAGGA GAA GACG AUG UAAA G UUAAUGAUGGU GC@LB@ \|\|\|\|\| \|\|\| \|\| \|\|\|\|\|:\| \|\|\| \|\|\|\| \|\|: \|\|\|\| \| \|\|\|\|\|\|\|\|\|:\| \|\|@LB@ CCCAU UUG CU AAGUCUU CUU CUGC UAU AUUU C AAUUACUACUA CG@LB@3'-UUU...UUAUC U GU UA A CAU UU U -5'@LB@ \| \|@LB@ 58 1@LB@@LB@@LB@Energy -12.17 kcal/mol@LB@Hybridization Energy -32.157 kcal/mol@LB@Unfolding Energy - mRNA 13.6506 kcal/mol@LB@Unfolding Energy - sRNA 6.33758 kcal/mol@LB@@LB@Position - mRNA 167 -- 226@LB@Position - sRNA 1 -- 57@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 4 -- 10@LB@
41	0.004182	0.3349	b3771	ilvD	-12.17	0.033695	175 -- 200	14 -- 53	dihydroxyacid dehydratase	_____________174______________________________________201\n_____________\|________________________________________\|\n5'-GCG...UUACG_________C_________________GA___________AUGCC...GUA-3'\n______________AGACAGACA_______________UGG__GUAAAUAAAGU\n______________\|:\|\|\|\|\|\|\|_______________:\|\|__:\|\|\|\|\|\|\|\|\|\|\n______________UUUGUCUGU_______________GCC__UAUUUAUUUCA\n3'-UUU...CCCCA_________AAGUCUUUACUUACU___AU___________AUUAC...UCG-5'\n_____________\|________________________________________\|\n_____________54_______________________________________13\n	194 -- 200	14 -- 20	-22.054 kcal/mol	3.56189 kcal/mol	6.32552 kcal/mol	174 201@LB@ \| \|@LB@5'-GCG...UUACG C GA AUGCC...GUA-3'@LB@ AGACAGACA UGG GUAAAUAAAGU@LB@ \|:\|\|\|\|\|\|\| :\|\| :\|\|\|\|\|\|\|\|\|\|@LB@ UUUGUCUGU GCC UAUUUAUUUCA@LB@3'-UUU...CCCCA AAGUCUUUACUUACU AU AUUAC...UCG-5'@LB@ \| \|@LB@ 54 13@LB@@LB@@LB@Energy -12.17 kcal/mol@LB@Hybridization Energy -22.054 kcal/mol@LB@Unfolding Energy - mRNA 3.56189 kcal/mol@LB@Unfolding Energy - sRNA 6.32552 kcal/mol@LB@@LB@Position - mRNA 175 -- 200@LB@Position - sRNA 14 -- 53@LB@Position Seed - mRNA 194 -- 200@LB@Position Seed - sRNA 14 -- 20@LB@
185	0.03096	0.5492	b1686	menI	-12.09	0.035132	263 -- 300	22 -- 58	1 4-dihydroxy-2-naphthoyl-CoA hydrolase	_____________262____________________________________300\n_____________\|______________________________________\|\n5'-GGC...AUGGU_____UCCU_________CUU_____CAUAU________-3'\n______________GGGGU____GGAUAUUCG____UGAA_____UGGUGAUG\n______________\|\|\|\|\|____:\|\|:\|\|\|\|:____\|\|\|\|_____:\|\|\|:\|\|:\n______________CCCCA____UCUGUAAGU____ACUU_____GCCAUUAU\n3'-UUU...UUUAU_____UUUG_________CUUU____ACU__________UUAUU...UCG-5'\n_____________\|_______________________________________\|\n_____________59______________________________________21\n	294 -- 300	22 -- 28	-26.9755 kcal/mol	8.57608 kcal/mol	6.31389 kcal/mol	262 300@LB@ \| \|@LB@5'-GGC...AUGGU UCCU CUU CAUAU -3'@LB@ GGGGU GGAUAUUCG UGAA UGGUGAUG@LB@ \|\|\|\|\| :\|\|:\|\|\|\|: \|\|\|\| :\|\|\|:\|\|:@LB@ CCCCA UCUGUAAGU ACUU GCCAUUAU@LB@3'-UUU...UUUAU UUUG CUUU ACU UUAUU...UCG-5'@LB@ \| \|@LB@ 59 21@LB@@LB@@LB@Energy -12.09 kcal/mol@LB@Hybridization Energy -26.9755 kcal/mol@LB@Unfolding Energy - mRNA 8.57608 kcal/mol@LB@Unfolding Energy - sRNA 6.31389 kcal/mol@LB@@LB@Position - mRNA 263 -- 300@LB@Position - sRNA 22 -- 58@LB@Position Seed - mRNA 294 -- 300@LB@Position Seed - sRNA 22 -- 28@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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