Input and runtime details for job 1229462 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1229462 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:25:41 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:59 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:22:57 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:23:04 CET 2018
	Job Completed		@ Fri Feb 16 20:23:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1229462&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicF

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
143	0.02405	0.526	b3292	zntR	-11.17	0.055543	275 -- 285	1 -- 12	zntA gene transcriptional activator	_____________274__________286\n_____________\|____________\|\n5'-UGA...CAGCA____________AUGAA...CUG-3'\n______________GAUGAUGG_AGC\n______________:\|\|\|\|\|\|:_\|\|\|\n______________UUACUACU_UCG\n3'-UUU...UUCAA________A___-5'\n_____________\|___________\|\n_____________13__________1\n	276 -- 282	5 -- 11	-12.3439 kcal/mol	1.13072 kcal/mol	0.0394463 kcal/mol	274 286@LB@ \| \|@LB@5'-UGA...CAGCA AUGAA...CUG-3'@LB@ GAUGAUGG AGC@LB@ :\|\|\|\|\|\|: \|\|\|@LB@ UUACUACU UCG@LB@3'-UUU...UUCAA A -5'@LB@ \| \|@LB@ 13 1@LB@@LB@@LB@Energy -11.17 kcal/mol@LB@Hybridization Energy -12.3439 kcal/mol@LB@Unfolding Energy - mRNA 1.13072 kcal/mol@LB@Unfolding Energy - sRNA 0.0394463 kcal/mol@LB@@LB@Position - mRNA 275 -- 285@LB@Position - sRNA 1 -- 12@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 5 -- 11@LB@
196	0.0332	0.554	b0128	yadH	-11.13	0.056665	104 -- 121	39 -- 55	putative ABC transporter permease	_____________103________________122\n_____________\|__________________\|\n5'-UUA...GUUAA_______G__________UAUUA...UCA-3'\n______________GUGAGCA_GGCAUUCAGG\n______________\|\|:\|:\|\|_\|:\|\|\|\|\|\|\|:\n______________CAUUUGU_CUGUAAGUCU\n3'-UUU...AUCCC__________________UUACU...UCG-5'\n_____________\|__________________\|\n_____________56_________________38\n	114 -- 120	40 -- 46	-20.6932 kcal/mol	3.311 kcal/mol	6.24918 kcal/mol	103 122@LB@ \| \|@LB@5'-UUA...GUUAA G UAUUA...UCA-3'@LB@ GUGAGCA GGCAUUCAGG@LB@ \|\|:\|:\|\| \|:\|\|\|\|\|\|\|:@LB@ CAUUUGU CUGUAAGUCU@LB@3'-UUU...AUCCC UUACU...UCG-5'@LB@ \| \|@LB@ 56 38@LB@@LB@@LB@Energy -11.13 kcal/mol@LB@Hybridization Energy -20.6932 kcal/mol@LB@Unfolding Energy - mRNA 3.311 kcal/mol@LB@Unfolding Energy - sRNA 6.24918 kcal/mol@LB@@LB@Position - mRNA 104 -- 121@LB@Position - sRNA 39 -- 55@LB@Position Seed - mRNA 114 -- 120@LB@Position Seed - sRNA 40 -- 46@LB@
144	0.02445	0.526	b3441	yhhY	-11.04	0.059403	187 -- 218	22 -- 53	aminoacyl nucleotide detoxifying acetyltransferase	_____________186________________________________219\n_____________\|__________________________________\|\n5'-CCC...CCCCA__GA_____A______________GAUA______CGCCA...UGC-3'\n______________AA__GGACA___AGGAAUGAGUGA____GUAAUA\n______________\|\|__:\|\|\|\|___\|\|:\|\|\|\|\|:\|\|\|____\|\|\|\|\|\|\n______________UU__UCUGU___UCUUUACUUACU____CAUUAU\n3'-UUU...CCCCA__UG_____AAG____________GC________UUAUU...UCG-5'\n_____________\|__________________________________\|\n_____________54_________________________________21\n	202 -- 208	30 -- 36	-22.9472 kcal/mol	5.59747 kcal/mol	6.31329 kcal/mol	186 219@LB@ \| \|@LB@5'-CCC...CCCCA GA A GAUA CGCCA...UGC-3'@LB@ AA GGACA AGGAAUGAGUGA GUAAUA@LB@ \|\| :\|\|\|\| \|\|:\|\|\|\|\|:\|\|\| \|\|\|\|\|\|@LB@ UU UCUGU UCUUUACUUACU CAUUAU@LB@3'-UUU...CCCCA UG AAG GC UUAUU...UCG-5'@LB@ \| \|@LB@ 54 21@LB@@LB@@LB@Energy -11.04 kcal/mol@LB@Hybridization Energy -22.9472 kcal/mol@LB@Unfolding Energy - mRNA 5.59747 kcal/mol@LB@Unfolding Energy - sRNA 6.31329 kcal/mol@LB@@LB@Position - mRNA 187 -- 218@LB@Position - sRNA 22 -- 53@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 30 -- 36@LB@
134	0.02184	0.526	b0034	caiF	-11.03	0.059446	251 -- 259	1 -- 9	cai operon transcriptional activator	_____________250_______260\n_____________\|_________\|\n5'-UGA...GAAUG_________UGAAA...AUU-3'\n______________GAUGAUGGC\n______________\|\|\|\|\|\|:\|\|\n______________CUACUAUCG\n3'-UUU...AAUUA_________-5'\n_____________\|________\|\n_____________10_______1\n	253 -- 259	1 -- 7	-13.9707 kcal/mol	2.91573 kcal/mol	0.0200155 kcal/mol	250 260@LB@ \| \|@LB@5'-UGA...GAAUG UGAAA...AUU-3'@LB@ GAUGAUGGC@LB@ \|\|\|\|\|\|:\|\|@LB@ CUACUAUCG@LB@3'-UUU...AAUUA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -13.9707 kcal/mol@LB@Unfolding Energy - mRNA 2.91573 kcal/mol@LB@Unfolding Energy - sRNA 0.0200155 kcal/mol@LB@@LB@Position - mRNA 251 -- 259@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 1 -- 7@LB@
31	0.00291	0.3081	b1205	ychH	-11.03	0.059536	152 -- 163	53 -- 64	DUF2583 family putative inner membrane protein	_____________151__________164\n_____________\|____________\|\n5'-CAA...CAUAA____G_______UUCCC...UAC-3'\n______________GGAA_AGGGGUA\n______________\|:\|\|_\|\|\|\|\|\|\|\n______________CUUU_UCCCCAU\n3'-UUU...GCCAA____A_______UUGUC...UCG-5'\n_____________\|____________\|\n_____________65___________52\n	157 -- 163	53 -- 59	-13.5677 kcal/mol	2.46117 kcal/mol	0.0747267 kcal/mol	151 164@LB@ \| \|@LB@5'-CAA...CAUAA G UUCCC...UAC-3'@LB@ GGAA AGGGGUA@LB@ \|:\|\| \|\|\|\|\|\|\|@LB@ CUUU UCCCCAU@LB@3'-UUU...GCCAA A UUGUC...UCG-5'@LB@ \| \|@LB@ 65 52@LB@@LB@@LB@Energy -11.03 kcal/mol@LB@Hybridization Energy -13.5677 kcal/mol@LB@Unfolding Energy - mRNA 2.46117 kcal/mol@LB@Unfolding Energy - sRNA 0.0747267 kcal/mol@LB@@LB@Position - mRNA 152 -- 163@LB@Position - sRNA 53 -- 64@LB@Position Seed - mRNA 157 -- 163@LB@Position Seed - sRNA 53 -- 59@LB@
130	0.02079	0.5217	b3455	livG	-10.98	0.060940	96 -- 122	4 -- 29	branched-chain amino acid ABC transporter ATPase	_____________95__________________________123\n_____________\|___________________________\|\n5'-AUG...AUGCU_______UU_____U_C__________CUGGC...UCG-3'\n______________CGGUGGU__GAUGG_G_UGAUGAUGAU\n______________\|\|\|\|::\|__:\|\|::_\|_\|:\|\|\|\|\|\|\|\|\n______________GCCAUUA__UUAUU_C_AUUACUACUA\n3'-UUU...UUACU_______U______U_A__________UCG-5'\n_____________\|___________________________\|\n_____________30__________________________3\n	116 -- 122	4 -- 10	-20.0623 kcal/mol	6.95142 kcal/mol	2.12698 kcal/mol	95 123@LB@ \| \|@LB@5'-AUG...AUGCU UU U C CUGGC...UCG-3'@LB@ CGGUGGU GAUGG G UGAUGAUGAU@LB@ \|\|\|\|::\| :\|\|:: \| \|:\|\|\|\|\|\|\|\|@LB@ GCCAUUA UUAUU C AUUACUACUA@LB@3'-UUU...UUACU U U A UCG-5'@LB@ \| \|@LB@ 30 3@LB@@LB@@LB@Energy -10.98 kcal/mol@LB@Hybridization Energy -20.0623 kcal/mol@LB@Unfolding Energy - mRNA 6.95142 kcal/mol@LB@Unfolding Energy - sRNA 2.12698 kcal/mol@LB@@LB@Position - mRNA 96 -- 122@LB@Position - sRNA 4 -- 29@LB@Position Seed - mRNA 116 -- 122@LB@Position Seed - sRNA 4 -- 10@LB@
108	0.01698	0.5123	b0078	ilvH	-10.97	0.061484	141 -- 180	1 -- 51	acetolactate synthase 3 small subunit valine-sensitive	_____________140_________________________________________________181\n_____________\|___________________________________________________\|\n5'-GCC...CCGAU______UCG___________________________AU_______UA____AAAAC...UGA-3'\n______________GCAGAU___CGGG_______GGCGG_AAUGGAUGAA____GUGGU___AGC\n______________:\|\|\|\|:___\|:\|:_______\|:\|\|\|_\|\|\|::\|\|:\|\|____:\|\|:\|___\|\|\|\n______________UGUCUG___GUCU_______CUGCC_UUAUUUAUUU____UACUA___UCG\n3'-UUU...CCAUU______UAA____UUACUUA_____A__________CAAU_____CUA___-5'\n_____________\|__________________________________________________\|\n_____________52_________________________________________________1\n	162 -- 168	16 -- 22	-27.7823 kcal/mol	10.4814 kcal/mol	6.33528 kcal/mol	140 181@LB@ \| \|@LB@5'-GCC...CCGAU UCG AU UA AAAAC...UGA-3'@LB@ GCAGAU CGGG GGCGG AAUGGAUGAA GUGGU AGC@LB@ :\|\|\|\|: \|:\|: \|:\|\|\| \|\|\|::\|\|:\|\| :\|\|:\| \|\|\|@LB@ UGUCUG GUCU CUGCC UUAUUUAUUU UACUA UCG@LB@3'-UUU...CCAUU UAA UUACUUA A CAAU CUA -5'@LB@ \| \|@LB@ 52 1@LB@@LB@@LB@Energy -10.97 kcal/mol@LB@Hybridization Energy -27.7823 kcal/mol@LB@Unfolding Energy - mRNA 10.4814 kcal/mol@LB@Unfolding Energy - sRNA 6.33528 kcal/mol@LB@@LB@Position - mRNA 141 -- 180@LB@Position - sRNA 1 -- 51@LB@Position Seed - mRNA 162 -- 168@LB@Position Seed - sRNA 16 -- 22@LB@
145	0.02447	0.526	b4518	ymdF	-10.96	0.061577	258 -- 296	23 -- 64	KGG family protein	_____________257________________________________________________297\n_____________\|__________________________________________________\|\n5'-UUU...CAUCA____C___UAAAAAA_________C___GC_____________G______UUCA-3'\n______________GAAG_AGG_______GGUGGACAG_ACA_____________GG_GGUAAU\n______________\|\|\|:_\|\|\|_______\|\|\|::\|\|\|\|_\|\|\|_____________\|:_\|\|\|\|\|\|\n______________CUUU_UCC_______CCAUUUGUC_UGU_____________CU_CCAUUA\n3'-UUU...GCCAA____A_______________________AAGUCUUUACUUA__G______UUUAU...UCG-5'\n_____________\|__________________________________________________\|\n_____________65_________________________________________________22\n	275 -- 281	48 -- 54	-22.543 kcal/mol	5.2615 kcal/mol	6.31898 kcal/mol	257 297@LB@ \| \|@LB@5'-UUU...CAUCA C UAAAAAA C GC G UUCA-3'@LB@ GAAG AGG GGUGGACAG ACA GG GGUAAU@LB@ \|\|\|: \|\|\| \|\|\|::\|\|\|\| \|\|\| \|: \|\|\|\|\|\|@LB@ CUUU UCC CCAUUUGUC UGU CU CCAUUA@LB@3'-UUU...GCCAA A AAGUCUUUACUUA G UUUAU...UCG-5'@LB@ \| \|@LB@ 65 22@LB@@LB@@LB@Energy -10.96 kcal/mol@LB@Hybridization Energy -22.543 kcal/mol@LB@Unfolding Energy - mRNA 5.2615 kcal/mol@LB@Unfolding Energy - sRNA 6.31898 kcal/mol@LB@@LB@Position - mRNA 258 -- 296@LB@Position - sRNA 23 -- 64@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 48 -- 54@LB@
33	0.003163	0.3086	b4372	holD	-10.86	0.064637	82 -- 98	44 -- 58	DNA polymerase III psi subunit	_____________81________________99\n_____________\|_________________\|\n5'-AGG...UUGCC_______AU________GGAAU...UCC-3'\n______________GGGGUAA__GCAGACAU\n______________\|\|\|\|\|\|\|__:\|\|\|\|\|\|\|\n______________CCCCAUU__UGUCUGUA\n3'-UUU...UUUAU_________________AGUCU...UCG-5'\n_____________\|_________________\|\n_____________59________________43\n	92 -- 98	44 -- 50	-21.4924 kcal/mol	4.96784 kcal/mol	5.66232 kcal/mol	81 99@LB@ \| \|@LB@5'-AGG...UUGCC AU GGAAU...UCC-3'@LB@ GGGGUAA GCAGACAU@LB@ \|\|\|\|\|\|\| :\|\|\|\|\|\|\|@LB@ CCCCAUU UGUCUGUA@LB@3'-UUU...UUUAU AGUCU...UCG-5'@LB@ \| \|@LB@ 59 43@LB@@LB@@LB@Energy -10.86 kcal/mol@LB@Hybridization Energy -21.4924 kcal/mol@LB@Unfolding Energy - mRNA 4.96784 kcal/mol@LB@Unfolding Energy - sRNA 5.66232 kcal/mol@LB@@LB@Position - mRNA 82 -- 98@LB@Position - sRNA 44 -- 58@LB@Position Seed - mRNA 92 -- 98@LB@Position Seed - sRNA 44 -- 50@LB@
68	0.00983	0.4523	b1321	ycjX	-10.80	0.066674	34 -- 41	55 -- 62	DUF463 family protein puatative P-loop NTPase	_____________33_______42\n_____________\|________\|\n5'-UGA...UAAAU________ACAUA...CAG-3'\n______________AGUAGGGG\n______________\|:\|\|\|\|\|\|\n______________UUAUCCCC\n3'-UUU...CAACU________AUUUG...UCG-5'\n_____________\|________\|\n_____________63_______54\n	35 -- 41	55 -- 61	-12.2037 kcal/mol	1.3787 kcal/mol	0.0270923 kcal/mol	33 42@LB@ \| \|@LB@5'-UGA...UAAAU ACAUA...CAG-3'@LB@ AGUAGGGG@LB@ \|:\|\|\|\|\|\|@LB@ UUAUCCCC@LB@3'-UUU...CAACU AUUUG...UCG-5'@LB@ \| \|@LB@ 63 54@LB@@LB@@LB@Energy -10.80 kcal/mol@LB@Hybridization Energy -12.2037 kcal/mol@LB@Unfolding Energy - mRNA 1.3787 kcal/mol@LB@Unfolding Energy - sRNA 0.0270923 kcal/mol@LB@@LB@Position - mRNA 34 -- 41@LB@Position - sRNA 55 -- 62@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 55 -- 61@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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