Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
196	0.01003	0.1776	b0344	lacZ	-8.86	0.369200	215 -- 237	48 -- 76	beta-D-galactosidase	_____________214___________________________238\n_____________\|_____________________________\|\n5'-ACC...AUUAC_____________________C_______AACGU...CUU-3'\n______________GGAUU_CAC_UGGC____CGU_GUUUUAC\n______________:\|\|:\|_\|\|\|_::\|\|____\|\|\|_\|\|\|\|:\|\|\n______________UCUGA_GUG_GUCG____GCA_CAAAGUG\n3'-UUU...CCGUU_____U___U____UUAA___A_______AGUCC...GCA-5'\n_____________\|_____________________________\|\n_____________77____________________________47\n	231 -- 237	48 -- 54	-17.9156 kcal/mol	5.37156 kcal/mol	3.68714 kcal/mol	214 238@LB@ \| \|@LB@5'-ACC...AUUAC C AACGU...CUU-3'@LB@ GGAUU CAC UGGC CGU GUUUUAC@LB@ :\|\|:\| \|\|\| ::\|\| \|\|\| \|\|\|\|:\|\|@LB@ UCUGA GUG GUCG GCA CAAAGUG@LB@3'-UUU...CCGUU U U UUAA A AGUCC...GCA-5'@LB@ \| \|@LB@ 77 47@LB@@LB@@LB@Energy -8.86 kcal/mol@LB@Hybridization Energy -17.9156 kcal/mol@LB@Unfolding Energy - mRNA 5.37156 kcal/mol@LB@Unfolding Energy - sRNA 3.68714 kcal/mol@LB@@LB@Position - mRNA 215 -- 237@LB@Position - sRNA 48 -- 76@LB@Position Seed - mRNA 231 -- 237@LB@Position Seed - sRNA 48 -- 54@LB@
43	0.00107	0.08759	b4036	lamB	-10.48	0.202041	185 -- 197	39 -- 51	maltose outer membrane porin (maltoporin)	_____________184___________198\n_____________\|_____________\|\n5'-CGC...AGCAA_G_______A___AGAAU...GUU-3'\n______________U_ACUCAGG_GAU\n______________\|_\|\|\|\|\|\|\|_\|\|\|\n______________A_UGAGUCC_CUA\n3'-UUU...AACAA_G_______C___AAGGU...GCA-5'\n_____________\|_____________\|\n_____________52____________38\n	187 -- 193	43 -- 49	-14.6452 kcal/mol	2.36402 kcal/mol	1.79807 kcal/mol	184 198@LB@ \| \|@LB@5'-CGC...AGCAA G A AGAAU...GUU-3'@LB@ U ACUCAGG GAU@LB@ \| \|\|\|\|\|\|\| \|\|\|@LB@ A UGAGUCC CUA@LB@3'-UUU...AACAA G C AAGGU...GCA-5'@LB@ \| \|@LB@ 52 38@LB@@LB@@LB@Energy -10.48 kcal/mol@LB@Hybridization Energy -14.6452 kcal/mol@LB@Unfolding Energy - mRNA 2.36402 kcal/mol@LB@Unfolding Energy - sRNA 1.79807 kcal/mol@LB@@LB@Position - mRNA 185 -- 197@LB@Position - sRNA 39 -- 51@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 43 -- 49@LB@
94	0.004209	0.1538	b1798	leuE	-8.23	0.450453	21 -- 61	47 -- 73	leucine efflux protein	_____________20_________________________________________62\n_____________\|__________________________________________\|\n5'-UGC...AAUUU____________U____CC_AGCACUACCCGAAUU_______GGAGC...AAA-3'\n______________UUACGC_CAGUA_UUUG__G_______________UUUCACU\n______________:\|\|\|:\|_\|\|\|:\|_\|\|:\|__\|_______________\|\|\|\|\|\|\|\n______________GAUGUG_GUCGU_AAGC__C_______________AAAGUGA\n3'-UUU...UUUCU______U_____U____AA_______________________GUCCC...GCA-5'\n_____________\|__________________________________________\|\n_____________74_________________________________________46\n	55 -- 61	47 -- 53	-16.3548 kcal/mol	4.43937 kcal/mol	3.69006 kcal/mol	20 62@LB@ \| \|@LB@5'-UGC...AAUUU U CC AGCACUACCCGAAUU GGAGC...AAA-3'@LB@ UUACGC CAGUA UUUG G UUUCACU@LB@ :\|\|\|:\| \|\|\|:\| \|\|:\| \| \|\|\|\|\|\|\|@LB@ GAUGUG GUCGU AAGC C AAAGUGA@LB@3'-UUU...UUUCU U U AA GUCCC...GCA-5'@LB@ \| \|@LB@ 74 46@LB@@LB@@LB@Energy -8.23 kcal/mol@LB@Hybridization Energy -16.3548 kcal/mol@LB@Unfolding Energy - mRNA 4.43937 kcal/mol@LB@Unfolding Energy - sRNA 3.69006 kcal/mol@LB@@LB@Position - mRNA 21 -- 61@LB@Position - sRNA 47 -- 73@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 47 -- 53@LB@
82	0.00343	0.1462	b4043	lexA	-10.80	0.176998	163 -- 196	40 -- 72	transcriptional repressor of SOS regulon	_____________162________________________________197\n_____________\|__________________________________\|\n5'-CGC...GUAUA___U_______UAAC___AUAUA___C_______CGGAA...AGC-3'\n______________UAC_CACAGCA____UGU_____CAC_CAGGGGG\n______________\|\|\|_\|\|\|\|\|\|\|____:\|\|_____\|\|\|_\|\|\|\|\|\|:\n______________AUG_GUGUCGU____GCA_____GUG_GUCCCCU\n3'-UUU...UUCUG___U_______UAA____ACAAA___A_______AAAGG...GCA-5'\n_____________\|__________________________________\|\n_____________73_________________________________39\n	190 -- 196	40 -- 46	-30.123 kcal/mol	14.5591 kcal/mol	4.76217 kcal/mol	162 197@LB@ \| \|@LB@5'-CGC...GUAUA U UAAC AUAUA C CGGAA...AGC-3'@LB@ UAC CACAGCA UGU CAC CAGGGGG@LB@ \|\|\| \|\|\|\|\|\|\| :\|\| \|\|\| \|\|\|\|\|\|:@LB@ AUG GUGUCGU GCA GUG GUCCCCU@LB@3'-UUU...UUCUG U UAA ACAAA A AAAGG...GCA-5'@LB@ \| \|@LB@ 73 39@LB@@LB@@LB@Energy -10.80 kcal/mol@LB@Hybridization Energy -30.123 kcal/mol@LB@Unfolding Energy - mRNA 14.5591 kcal/mol@LB@Unfolding Energy - sRNA 4.76217 kcal/mol@LB@@LB@Position - mRNA 163 -- 196@LB@Position - sRNA 40 -- 72@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 40 -- 46@LB@
188	0.009562	0.1776	b0147	ligT	-10.24	0.223023	2 -- 33	35 -- 66	2'-5' RNA ligase	_____________1_________________________________34\n_____________\|_________________________________\|\n__________5'-C________GCAGCGG___GC____UU_______CGGCC...CGG-3'\n______________CAGCAGUU________CA__CAGG__GAUUUCC\n______________\|\|\|\|\|:\|\|________\|\|__\|\|\|\|__\|\|\|\|\|\|\|\n______________GUCGUUAA________GU__GUCC__CUAAAGG\n3'-UUU...UGUGU________GCAACAAA__GA____C________UACGA...GCA-5'\n_____________\|_________________________________\|\n_____________67________________________________34\n	27 -- 33	35 -- 41	-25.6384 kcal/mol	10.64 kcal/mol	4.7609 kcal/mol	1 34@LB@ \| \|@LB@ 5'-C GCAGCGG GC UU CGGCC...CGG-3'@LB@ CAGCAGUU CA CAGG GAUUUCC@LB@ \|\|\|\|\|:\|\| \|\| \|\|\|\| \|\|\|\|\|\|\|@LB@ GUCGUUAA GU GUCC CUAAAGG@LB@3'-UUU...UGUGU GCAACAAA GA C UACGA...GCA-5'@LB@ \| \|@LB@ 67 34@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -25.6384 kcal/mol@LB@Unfolding Energy - mRNA 10.64 kcal/mol@LB@Unfolding Energy - sRNA 4.7609 kcal/mol@LB@@LB@Position - mRNA 2 -- 33@LB@Position - sRNA 35 -- 66@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 35 -- 41@LB@
67	0.002719	0.1417	b3454	livF	-11.67	0.120634	106 -- 122	40 -- 58	branched-chain amino acid ABC transporter ATPase	_____________105_________________123\n_____________\|___________________\|\n5'-AGA...AUUUA___GG_____A________CGCCG...UUG-3'\n______________CGU____UCA_UCAGGGGA\n______________\|\|\|____\|\|\|_\|\|\|\|\|\|\|\|\n______________GCA____AGU_AGUCCCCU\n3'-UUU...GUUAA___ACAA___G________AAAGG...GCA-5'\n_____________\|___________________\|\n_____________59__________________39\n	116 -- 122	40 -- 46	-19.8465 kcal/mol	5.75116 kcal/mol	2.42241 kcal/mol	105 123@LB@ \| \|@LB@5'-AGA...AUUUA GG A CGCCG...UUG-3'@LB@ CGU UCA UCAGGGGA@LB@ \|\|\| \|\|\| \|\|\|\|\|\|\|\|@LB@ GCA AGU AGUCCCCU@LB@3'-UUU...GUUAA ACAA G AAAGG...GCA-5'@LB@ \| \|@LB@ 59 39@LB@@LB@@LB@Energy -11.67 kcal/mol@LB@Hybridization Energy -19.8465 kcal/mol@LB@Unfolding Energy - mRNA 5.75116 kcal/mol@LB@Unfolding Energy - sRNA 2.42241 kcal/mol@LB@@LB@Position - mRNA 106 -- 122@LB@Position - sRNA 40 -- 58@LB@Position Seed - mRNA 116 -- 122@LB@Position Seed - sRNA 40 -- 46@LB@
10	6.564e-05	0.0231	b3458	livK	-17.14	0.005196	153 -- 196	35 -- 71	leucine transporter subunit	_____________152____________________________________________197\n_____________\|______________________________________________\|\n5'-UGU...UGUUA______AAAAACAA_____CACAACA______GAAU__________GACUA...CGA-3'\n______________ACACAU________AGCAA_________UCAC____GGGGAUUUUU\n______________\|\|\|\|\|:________\|\|\|\|\|_________\|\|\|\|____\|\|\|\|\|\|\|\|::\n______________UGUGUG________UCGUU_________AGUG____CCCCUAAAGG\n3'-UUU...UCUGA___________________AAGCAACAA____AGU___________UACGA...GCA-5'\n_____________\|______________________________________________\|\n_____________72_____________________________________________34\n	190 -- 196	35 -- 41	-26.3585 kcal/mol	4.43917 kcal/mol	4.78171 kcal/mol	152 197@LB@ \| \|@LB@5'-UGU...UGUUA AAAAACAA CACAACA GAAU GACUA...CGA-3'@LB@ ACACAU AGCAA UCAC GGGGAUUUUU@LB@ \|\|\|\|\|: \|\|\|\|\| \|\|\|\| \|\|\|\|\|\|\|\|::@LB@ UGUGUG UCGUU AGUG CCCCUAAAGG@LB@3'-UUU...UCUGA AAGCAACAA AGU UACGA...GCA-5'@LB@ \| \|@LB@ 72 34@LB@@LB@@LB@Energy -17.14 kcal/mol@LB@Hybridization Energy -26.3585 kcal/mol@LB@Unfolding Energy - mRNA 4.43917 kcal/mol@LB@Unfolding Energy - sRNA 4.78171 kcal/mol@LB@@LB@Position - mRNA 153 -- 196@LB@Position - sRNA 35 -- 71@LB@Position Seed - mRNA 190 -- 196@LB@Position Seed - sRNA 35 -- 41@LB@
99	0.004523	0.1538	b1516	lsrB	-10.33	0.215118	8 -- 28	39 -- 59	autoinducer 2-binding protein	_____________7______________________29\n_____________\|______________________\|\n____5'-ACCGCCA___CG___________U_____AUUUG...UUC-3'\n______________UUG__GUUUUAUU_AG_GGGAU\n______________\|:\|__\|\|\|\|:\|:\|_\|\|_\|\|\|\|\|\n______________AGC__CAAAGUGA_UC_CCCUA\n3'-UUU...CGUUA___AA________G________AAGGU...GCA-5'\n_____________\|______________________\|\n_____________60_____________________38\n	14 -- 20	47 -- 53	-16.4048 kcal/mol	3.58309 kcal/mol	2.49358 kcal/mol	7 29@LB@ \| \|@LB@ 5'-ACCGCCA CG U AUUUG...UUC-3'@LB@ UUG GUUUUAUU AG GGGAU@LB@ \|:\| \|\|\|\|:\|:\| \|\| \|\|\|\|\|@LB@ AGC CAAAGUGA UC CCCUA@LB@3'-UUU...CGUUA AA G AAGGU...GCA-5'@LB@ \| \|@LB@ 60 38@LB@@LB@@LB@Energy -10.33 kcal/mol@LB@Hybridization Energy -16.4048 kcal/mol@LB@Unfolding Energy - mRNA 3.58309 kcal/mol@LB@Unfolding Energy - sRNA 2.49358 kcal/mol@LB@@LB@Position - mRNA 8 -- 28@LB@Position - sRNA 39 -- 59@LB@Position Seed - mRNA 14 -- 20@LB@Position Seed - sRNA 47 -- 53@LB@
194	0.009812	0.1776	b2687	luxS	-7.90	0.495572	99 -- 106	42 -- 49	S-ribosylhomocysteine lyase	_____________98_______107\n_____________\|________\|\n5'-GAA...UCUGA________AUGAU...UCA-3'\n______________AUUCAGGG\n______________\|:\|\|\|\|\|\|\n______________UGAGUCCC\n3'-UUU...CAAAG________CUAAA...GCA-5'\n_____________\|________\|\n_____________50_______41\n	100 -- 106	42 -- 48	-12.5942 kcal/mol	3.05922 kcal/mol	1.63991 kcal/mol	98 107@LB@ \| \|@LB@5'-GAA...UCUGA AUGAU...UCA-3'@LB@ AUUCAGGG@LB@ \|:\|\|\|\|\|\|@LB@ UGAGUCCC@LB@3'-UUU...CAAAG CUAAA...GCA-5'@LB@ \| \|@LB@ 50 41@LB@@LB@@LB@Energy -7.90 kcal/mol@LB@Hybridization Energy -12.5942 kcal/mol@LB@Unfolding Energy - mRNA 3.05922 kcal/mol@LB@Unfolding Energy - sRNA 1.63991 kcal/mol@LB@@LB@Position - mRNA 99 -- 106@LB@Position - sRNA 42 -- 49@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 42 -- 48@LB@
163	0.007762	0.1668	b0394	mak	-14.23	0.032787	242 -- 265	40 -- 75	manno(fructo)kinase	_____________241__________________________________266\n_____________\|____________________________________\|\n5'-GUU...GAAGU________UG______G___________________GCAGU...GUG-3'\n______________GAUUGCAC__GGCGAU_____________CAGGGGA\n______________\|\|:\|:\|\|\|__:\|\|:\|\|_____________\|\|\|\|\|\|\|\n______________CUGAUGUG__UCGUUA_____________GUCCCCU\n3'-UUU...CGUUU________UG______AGCAACAAAGUGA_______AAAGG...GCA-5'\n_____________\|____________________________________\|\n_____________76___________________________________39\n	259 -- 265	40 -- 46	-27.5886 kcal/mol	8.58508 kcal/mol	4.77786 kcal/mol	241 266@LB@ \| \|@LB@5'-GUU...GAAGU UG G GCAGU...GUG-3'@LB@ GAUUGCAC GGCGAU CAGGGGA@LB@ \|\|:\|:\|\|\| :\|\|:\|\| \|\|\|\|\|\|\|@LB@ CUGAUGUG UCGUUA GUCCCCU@LB@3'-UUU...CGUUU UG AGCAACAAAGUGA AAAGG...GCA-5'@LB@ \| \|@LB@ 76 39@LB@@LB@@LB@Energy -14.23 kcal/mol@LB@Hybridization Energy -27.5886 kcal/mol@LB@Unfolding Energy - mRNA 8.58508 kcal/mol@LB@Unfolding Energy - sRNA 4.77786 kcal/mol@LB@@LB@Position - mRNA 242 -- 265@LB@Position - sRNA 40 -- 75@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 40 -- 46@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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