Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
26	0.0002514	0.03403	b4551	yheV	-11.83	0.112444	191 -- 229	42 -- 77	DUF2387 family putative metal-binding protein	_____________190_____________________________________230\n_____________\|_______________________________________\|\n5'-AUC...GAGUA____G__A_G________C__AAAAC________GC___CGCAA...UUG-3'\n______________GAGG_UA_A_AUGGCAAU_CG_____GUUUUAUU__GGG\n______________:\|\|:_\|\|_\|_\|::\|\|\|\|\|_\|\|_____\|\|\|\|:\|:\|__\|\|\|\n______________UUCU_AU_U_UGUCGUUA_GC_____CAAAGUGA__CCC\n3'-UUU...CCCGU____G__G_G________A__AA___________GU___CUAAA...GCA-5'\n_____________\|_______________________________________\|\n_____________78______________________________________41\n	217 -- 223	48 -- 54	-24.6454 kcal/mol	8.05545 kcal/mol	4.7648 kcal/mol	190 230@LB@ \| \|@LB@5'-AUC...GAGUA G A G C AAAAC GC CGCAA...UUG-3'@LB@ GAGG UA A AUGGCAAU CG GUUUUAUU GGG@LB@ :\|\|: \|\| \| \|::\|\|\|\|\| \|\| \|\|\|\|:\|:\| \|\|\|@LB@ UUCU AU U UGUCGUUA GC CAAAGUGA CCC@LB@3'-UUU...CCCGU G G G A AA GU CUAAA...GCA-5'@LB@ \| \|@LB@ 78 41@LB@@LB@@LB@Energy -11.83 kcal/mol@LB@Hybridization Energy -24.6454 kcal/mol@LB@Unfolding Energy - mRNA 8.05545 kcal/mol@LB@Unfolding Energy - sRNA 4.7648 kcal/mol@LB@@LB@Position - mRNA 191 -- 229@LB@Position - sRNA 42 -- 77@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 48 -- 54@LB@
132	0.005915	0.1577	b3356	yhfA	-11.83	0.112173	273 -- 293	39 -- 71	OsmC family protein	_____________272_______________________________294\n_____________\|_________________________________\|\n5'-CUG...UUUUA___G_________________________C___AAAGCAC-3'\n______________AUG_ACGGCA____________ACUCAGG_GAU\n______________\|::_\|\|:\|\|\|____________\|\|\|\|\|\|\|_\|\|\|\n______________UGU_UGUCGU____________UGAGUCC_CUA\n3'-UUU...UCUGA___G______UAAGCAACAAAG_______C___AAGGU...GCA-5'\n_____________\|_________________________________\|\n_____________72________________________________38\n	283 -- 289	43 -- 49	-20.9729 kcal/mol	4.393 kcal/mol	4.74947 kcal/mol	272 294@LB@ \| \|@LB@5'-CUG...UUUUA G C AAAGCAC-3'@LB@ AUG ACGGCA ACUCAGG GAU@LB@ \|:: \|\|:\|\|\| \|\|\|\|\|\|\| \|\|\|@LB@ UGU UGUCGU UGAGUCC CUA@LB@3'-UUU...UCUGA G UAAGCAACAAAG C AAGGU...GCA-5'@LB@ \| \|@LB@ 72 38@LB@@LB@@LB@Energy -11.83 kcal/mol@LB@Hybridization Energy -20.9729 kcal/mol@LB@Unfolding Energy - mRNA 4.393 kcal/mol@LB@Unfolding Energy - sRNA 4.74947 kcal/mol@LB@@LB@Position - mRNA 273 -- 293@LB@Position - sRNA 39 -- 71@LB@Position Seed - mRNA 283 -- 289@LB@Position Seed - sRNA 43 -- 49@LB@
51	0.001671	0.1153	b3527	yhjJ	-13.34	0.053290	153 -- 204	31 -- 75	putative periplasmic M16 family chaperone	_____________152_____________________________________________________205\n_____________\|_______________________________________________________\|\n5'-CUC...UGACC___A___G_____UU___UAUUACCAGGAUA___GA____________CA_____AGGGC...GGC-3'\n______________GAC_ACG_GCAGU__UUU_____________GUU____UCAGGGG_UU__CAUGC\n______________\|\|\|_\|\|:_:\|\|\|:__\|\|:_____________\|\|\|____\|\|\|\|\|\|\|_\|\|__\|\|\|\|\|\n______________CUG_UGU_UGUCG__AAG_____________CAA____AGUCCCC_AA__GUACG\n3'-UUU...CGUUU___A___G_____UU___CAA_____________AGUG_______U__AG_____AAUAU...GCA-5'\n_____________\|_______________________________________________________\|\n_____________76______________________________________________________30\n	189 -- 195	41 -- 47	-32.6504 kcal/mol	12.0549 kcal/mol	7.26183 kcal/mol	152 205@LB@ \| \|@LB@5'-CUC...UGACC A G UU UAUUACCAGGAUA GA CA AGGGC...GGC-3'@LB@ GAC ACG GCAGU UUU GUU UCAGGGG UU CAUGC@LB@ \|\|\| \|\|: :\|\|\|: \|\|: \|\|\| \|\|\|\|\|\|\| \|\| \|\|\|\|\|@LB@ CUG UGU UGUCG AAG CAA AGUCCCC AA GUACG@LB@3'-UUU...CGUUU A G UU CAA AGUG U AG AAUAU...GCA-5'@LB@ \| \|@LB@ 76 30@LB@@LB@@LB@Energy -13.34 kcal/mol@LB@Hybridization Energy -32.6504 kcal/mol@LB@Unfolding Energy - mRNA 12.0549 kcal/mol@LB@Unfolding Energy - sRNA 7.26183 kcal/mol@LB@@LB@Position - mRNA 153 -- 204@LB@Position - sRNA 31 -- 75@LB@Position Seed - mRNA 189 -- 195@LB@Position Seed - sRNA 41 -- 47@LB@
103	0.004595	0.1538	b3554	yiaF	-10.61	0.191588	8 -- 38	46 -- 75	barrier effect co-colonization resistance factor; DUF3053 family lipoprotein	_____________7_________________________________39\n_____________\|_________________________________\|\n____5'-AAUCUCU_______AAAAA_________GCACUA______UUUAA...AGC-3'\n______________GGCUACA_____GGCAA__CG______UCAUUU\n______________\|:\|\|\|\|\|_____:\|\|\|\|__\|\|______\|\|\|:\|:\n______________CUGAUGU_____UCGUU__GC______AGUGAG\n3'-UUU...CGUUU_______GUG_______AA__AACAA_______UCCCC...GCA-5'\n_____________\|_________________________________\|\n_____________76________________________________45\n	8 -- 14	69 -- 75	-17.9003 kcal/mol	3.5618 kcal/mol	3.72616 kcal/mol	7 39@LB@ \| \|@LB@ 5'-AAUCUCU AAAAA GCACUA UUUAA...AGC-3'@LB@ GGCUACA GGCAA CG UCAUUU@LB@ \|:\|\|\|\|\| :\|\|\|\| \|\| \|\|\|:\|:@LB@ CUGAUGU UCGUU GC AGUGAG@LB@3'-UUU...CGUUU GUG AA AACAA UCCCC...GCA-5'@LB@ \| \|@LB@ 76 45@LB@@LB@@LB@Energy -10.61 kcal/mol@LB@Hybridization Energy -17.9003 kcal/mol@LB@Unfolding Energy - mRNA 3.5618 kcal/mol@LB@Unfolding Energy - sRNA 3.72616 kcal/mol@LB@@LB@Position - mRNA 8 -- 38@LB@Position - sRNA 46 -- 75@LB@Position Seed - mRNA 8 -- 14@LB@Position Seed - sRNA 69 -- 75@LB@
62	0.002558	0.1417	b3579	yiaO	-11.31	0.142375	245 -- 258	62 -- 75	2 3-diketo-L-gulonate-binding periplasmic protein	_____________244____________259\n_____________\|______________\|\n5'-ACU...GGCCU________U_____CAUCU...AAA-3'\n______________GGCUGCAU_CAGCA\n______________\|:\|\|:\|\|:_\|\|\|\|\|\n______________CUGAUGUG_GUCGU\n3'-UUU...CGUUU________U_____UAAGC...GCA-5'\n_____________\|______________\|\n_____________76_____________61\n	246 -- 252	68 -- 74	-19.3904 kcal/mol	4.83304 kcal/mol	3.25167 kcal/mol	244 259@LB@ \| \|@LB@5'-ACU...GGCCU U CAUCU...AAA-3'@LB@ GGCUGCAU CAGCA@LB@ \|:\|\|:\|\|: \|\|\|\|\|@LB@ CUGAUGUG GUCGU@LB@3'-UUU...CGUUU U UAAGC...GCA-5'@LB@ \| \|@LB@ 76 61@LB@@LB@@LB@Energy -11.31 kcal/mol@LB@Hybridization Energy -19.3904 kcal/mol@LB@Unfolding Energy - mRNA 4.83304 kcal/mol@LB@Unfolding Energy - sRNA 3.25167 kcal/mol@LB@@LB@Position - mRNA 245 -- 258@LB@Position - sRNA 62 -- 75@LB@Position Seed - mRNA 246 -- 252@LB@Position Seed - sRNA 68 -- 74@LB@
72	0.002899	0.1417	b3688	yidQ	-12.98	0.064171	231 -- 261	39 -- 71	DUF1375 family outer membrane protein	_____________230_______________________________262\n_____________\|_________________________________\|\n5'-AUG...UCAUG___________AAUGACA__________A____GCUCC...GAA-3'\n______________AUAUGCAGCGG_________UUACUCGG_GGAU\n______________\|:\|::\|\|\|\|::_________\|:\|\|\|\|:\|_\|\|\|\|\n______________UGUGUGUCGUU_________AGUGAGUC_CCUA\n3'-UUU...UCUGA___________AAGCAACAA________C____AAGGU...GCA-5'\n_____________\|_________________________________\|\n_____________72________________________________38\n	250 -- 256	44 -- 50	-25.1937 kcal/mol	7.46905 kcal/mol	4.74947 kcal/mol	230 262@LB@ \| \|@LB@5'-AUG...UCAUG AAUGACA A GCUCC...GAA-3'@LB@ AUAUGCAGCGG UUACUCGG GGAU@LB@ \|:\|::\|\|\|\|:: \|:\|\|\|\|:\| \|\|\|\|@LB@ UGUGUGUCGUU AGUGAGUC CCUA@LB@3'-UUU...UCUGA AAGCAACAA C AAGGU...GCA-5'@LB@ \| \|@LB@ 72 38@LB@@LB@@LB@Energy -12.98 kcal/mol@LB@Hybridization Energy -25.1937 kcal/mol@LB@Unfolding Energy - mRNA 7.46905 kcal/mol@LB@Unfolding Energy - sRNA 4.74947 kcal/mol@LB@@LB@Position - mRNA 231 -- 261@LB@Position - sRNA 39 -- 71@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 44 -- 50@LB@
192	0.00979	0.1776	b3879	yihR	-14.50	0.028125	239 -- 282	38 -- 72	putative sulphoquinovose mutarotase	_____________238__________________________________________283\n_____________\|____________________________________________\|\n5'-CAU...ACCAA_______U_____GGACAGACC________UGCCG_________AUCAC...UGA-3'\n______________UAUGCAU_GCAGU_________GUUUCACU_____CGGGGGAUU\n______________\|\|::\|\|:_\|\|\|:\|_________\|\|\|\|\|\|\|\|_____\|:\|\|\|\|\|\|\|\n______________AUGUGUG_CGUUA_________CAAAGUGA_____GUCCCCUAA\n3'-UUU...UUCUG_______U_____AGCAA__________________________AGGUA...GCA-5'\n_____________\|____________________________________________\|\n_____________73___________________________________________37\n	276 -- 282	38 -- 44	-32.397 kcal/mol	13.138 kcal/mol	4.76382 kcal/mol	238 283@LB@ \| \|@LB@5'-CAU...ACCAA U GGACAGACC UGCCG AUCAC...UGA-3'@LB@ UAUGCAU GCAGU GUUUCACU CGGGGGAUU@LB@ \|\|::\|\|: \|\|\|:\| \|\|\|\|\|\|\|\| \|:\|\|\|\|\|\|\|@LB@ AUGUGUG CGUUA CAAAGUGA GUCCCCUAA@LB@3'-UUU...UUCUG U AGCAA AGGUA...GCA-5'@LB@ \| \|@LB@ 73 37@LB@@LB@@LB@Energy -14.50 kcal/mol@LB@Hybridization Energy -32.397 kcal/mol@LB@Unfolding Energy - mRNA 13.138 kcal/mol@LB@Unfolding Energy - sRNA 4.76382 kcal/mol@LB@@LB@Position - mRNA 239 -- 282@LB@Position - sRNA 38 -- 72@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 38 -- 44@LB@
70	0.002881	0.1417	b4144	yjeI	-14.17	0.033933	177 -- 235	31 -- 99	DUF4156 family lipoprotein	_____________176______________________________________________________________________236\n_____________\|________________________________________________________________________\|\n5'-GCA...CGUCC_________AUU________G__UA______________AA_______________CA______G__G____CGCGC...GCA-3'\n______________AGCUCAUUG______AUGGG_AA__AC_AUGCAC_GUAA__________UACUUAG__GGGAUU_UC_GUGC\n______________\|\|\|:\|\|\|:\|______\|\|\|\|\|_\|\|__\|\|_\|::\|\|\|_\|:\|\|__________:\|\|\|:\|\|__\|\|\|\|\|\|_\|\|_:\|\|\|\n______________UCGGGUAGC______UACCC_UU__UG_UGUGUG_CGUU__________GUGAGUC__CCCUAA_AG_UACG\n_____3'-UUUUUU_________ACCCUC_____G__UC__A______U____AAGCAACAAA__________________G____AAUAU...GCA-5'\n_____________\|________________________________________________________________________\|\n_____________100______________________________________________________________________30\n	213 -- 219	44 -- 50	-46.9384 kcal/mol	12.7048 kcal/mol	20.0691 kcal/mol	176 236@LB@ \| \|@LB@5'-GCA...CGUCC AUU G UA AA CA G G CGCGC...GCA-3'@LB@ AGCUCAUUG AUGGG AA AC AUGCAC GUAA UACUUAG GGGAUU UC GUGC@LB@ \|\|\|:\|\|\|:\| \|\|\|\|\| \|\| \|\| \|::\|\|\| \|:\|\| :\|\|\|:\|\| \|\|\|\|\|\| \|\| :\|\|\|@LB@ UCGGGUAGC UACCC UU UG UGUGUG CGUU GUGAGUC CCCUAA AG UACG@LB@ 3'-UUUUUU ACCCUC G UC A U AAGCAACAAA G AAUAU...GCA-5'@LB@ \| \|@LB@ 100 30@LB@@LB@@LB@Energy -14.17 kcal/mol@LB@Hybridization Energy -46.9384 kcal/mol@LB@Unfolding Energy - mRNA 12.7048 kcal/mol@LB@Unfolding Energy - sRNA 20.0691 kcal/mol@LB@@LB@Position - mRNA 177 -- 235@LB@Position - sRNA 31 -- 99@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 44 -- 50@LB@
83	0.003448	0.1462	b4231	yjfF	-16.06	0.010834	60 -- 72	39 -- 51	putative sugar ABC transporter permease	_____________59____________73\n_____________\|_____________\|\n5'-CGG...GCUGA_____________GAACA...GAG-3'\n______________UUAUUCAGGGGAU\n______________\|:\|:\|\|\|\|\|\|\|\|\|\n______________AGUGAGUCCCCUA\n3'-UUU...AACAA_____________AAGGU...GCA-5'\n_____________\|_____________\|\n_____________52____________38\n	66 -- 72	39 -- 45	-20.8743 kcal/mol	3.02031 kcal/mol	1.79807 kcal/mol	59 73@LB@ \| \|@LB@5'-CGG...GCUGA GAACA...GAG-3'@LB@ UUAUUCAGGGGAU@LB@ \|:\|:\|\|\|\|\|\|\|\|\|@LB@ AGUGAGUCCCCUA@LB@3'-UUU...AACAA AAGGU...GCA-5'@LB@ \| \|@LB@ 52 38@LB@@LB@@LB@Energy -16.06 kcal/mol@LB@Hybridization Energy -20.8743 kcal/mol@LB@Unfolding Energy - mRNA 3.02031 kcal/mol@LB@Unfolding Energy - sRNA 1.79807 kcal/mol@LB@@LB@Position - mRNA 60 -- 72@LB@Position - sRNA 39 -- 51@LB@Position Seed - mRNA 66 -- 72@LB@Position Seed - sRNA 39 -- 45@LB@
155	0.007577	0.1668	b4364	yjjP	-9.15	0.334468	166 -- 178	62 -- 74	DUF1212 family inner membrane protein	_____________165___________179\n_____________\|_____________\|\n5'-UCA...UCUGA__A__________CGUCU...UUU-3'\n______________AC_AUACGCAGCA\n______________\|\|_\|:\|\|:\|\|\|\|\|\n______________UG_UGUGUGUCGU\n3'-UUU...GUUUC__A__________UAAGC...GCA-5'\n_____________\|_____________\|\n_____________75____________61\n	172 -- 178	62 -- 68	-14.8746 kcal/mol	2.48219 kcal/mol	3.24359 kcal/mol	165 179@LB@ \| \|@LB@5'-UCA...UCUGA A CGUCU...UUU-3'@LB@ AC AUACGCAGCA@LB@ \|\| \|:\|\|:\|\|\|\|\|@LB@ UG UGUGUGUCGU@LB@3'-UUU...GUUUC A UAAGC...GCA-5'@LB@ \| \|@LB@ 75 61@LB@@LB@@LB@Energy -9.15 kcal/mol@LB@Hybridization Energy -14.8746 kcal/mol@LB@Unfolding Energy - mRNA 2.48219 kcal/mol@LB@Unfolding Energy - sRNA 3.24359 kcal/mol@LB@@LB@Position - mRNA 166 -- 178@LB@Position - sRNA 62 -- 74@LB@Position Seed - mRNA 172 -- 178@LB@Position Seed - sRNA 62 -- 68@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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