Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
146	0.01469	0.3231	b0026	ileS	-7.82	0.067581	147 -- 206	2 -- 59	isoleucyl-tRNA synthetase	_____________146_____________________________________________________________207\n_____________\|_______________________________________________________________\|\n5'-AUG...UAACA____CG________CU_UUAU___AUCAAACC_____A__GA______A___________A__GACUA...CUG-3'\n______________AAAC__GCUUAAGC__G____GUA________GAAAU_CG____ACCG__GAAUCUGAUG_GU\n______________\|\|\|\|__\|\|\|\|\|\|\|\|__\|____\|:\|________:\|\|\|\|_\|\|____\|\|\|:__:\|\|\|\|\|\|\|\|\|_\|\|\n______________UUUG__CGAAUUCG__C____CGU________UUUUA_GC____UGGU__UUUAGACUAC_CA\n3'-UUU...CCUAC____AA________UU_UU_____GAAUU________A__AAUG____CC__________A__A-5'\n_____________\|_______________________________________________________________\|\n_____________60______________________________________________________________1\n	197 -- 203	5 -- 11	-33.6314 kcal/mol	11.9684 kcal/mol	13.8468 kcal/mol	146 207@LB@ \| \|@LB@5'-AUG...UAACA CG CU UUAU AUCAAACC A GA A A GACUA...CUG-3'@LB@ AAAC GCUUAAGC G GUA GAAAU CG ACCG GAAUCUGAUG GU@LB@ \|\|\|\| \|\|\|\|\|\|\|\| \| \|:\| :\|\|\|\| \|\| \|\|\|: :\|\|\|\|\|\|\|\|\| \|\|@LB@ UUUG CGAAUUCG C CGU UUUUA GC UGGU UUUAGACUAC CA@LB@3'-UUU...CCUAC AA UU UU GAAUU A AAUG CC A A-5'@LB@ \| \|@LB@ 60 1@LB@@LB@@LB@Energy -7.82 kcal/mol@LB@Hybridization Energy -33.6314 kcal/mol@LB@Unfolding Energy - mRNA 11.9684 kcal/mol@LB@Unfolding Energy - sRNA 13.8468 kcal/mol@LB@@LB@Position - mRNA 147 -- 206@LB@Position - sRNA 2 -- 59@LB@Position Seed - mRNA 197 -- 203@LB@Position Seed - sRNA 5 -- 11@LB@
188	0.02199	0.3768	b3774	ilvC	-7.43	0.085257	172 -- 181	37 -- 46	ketol-acid reductoisomerase NAD(P)-binding	_____________171________182\n_____________\|__________\|\n5'-AUG...AACAA__________AACAU...GCU-3'\n______________UAAGAAGCAC\n______________:\|\|\|\|\|\|\|\|\|\n______________GUUCUUCGUG\n3'-UUU...AAUUC__________AAUUU...CAA-5'\n_____________\|__________\|\n_____________47_________36\n	175 -- 181	37 -- 43	-13.663 kcal/mol	1.01181 kcal/mol	5.21654 kcal/mol	171 182@LB@ \| \|@LB@5'-AUG...AACAA AACAU...GCU-3'@LB@ UAAGAAGCAC@LB@ :\|\|\|\|\|\|\|\|\|@LB@ GUUCUUCGUG@LB@3'-UUU...AAUUC AAUUU...CAA-5'@LB@ \| \|@LB@ 47 36@LB@@LB@@LB@Energy -7.43 kcal/mol@LB@Hybridization Energy -13.663 kcal/mol@LB@Unfolding Energy - mRNA 1.01181 kcal/mol@LB@Unfolding Energy - sRNA 5.21654 kcal/mol@LB@@LB@Position - mRNA 172 -- 181@LB@Position - sRNA 37 -- 46@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 37 -- 43@LB@
24	0.000694	0.09021	b2531	iscR	-7.21	0.097486	53 -- 65	27 -- 41	isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress- and iron starvation-inducible; autorepressor	_____________52______________66\n_____________\|_______________\|\n5'-AUG...AAAAU_______________AAAUA...AAC-3'\n______________GGCACU__GAAGGUU\n______________:\|\|\|\|\|__:\|\|::\|\|\n______________UCGUGA__UUUUUAA\n3'-UUU...GUUCU______AU_______GCAAU...CAA-5'\n_____________\|_______________\|\n_____________42______________26\n	59 -- 65	27 -- 33	-10.1429 kcal/mol	1.29 kcal/mol	1.6427 kcal/mol	52 66@LB@ \| \|@LB@5'-AUG...AAAAU AAAUA...AAC-3'@LB@ GGCACU GAAGGUU@LB@ :\|\|\|\|\| :\|\|::\|\|@LB@ UCGUGA UUUUUAA@LB@3'-UUU...GUUCU AU GCAAU...CAA-5'@LB@ \| \|@LB@ 42 26@LB@@LB@@LB@Energy -7.21 kcal/mol@LB@Hybridization Energy -10.1429 kcal/mol@LB@Unfolding Energy - mRNA 1.29 kcal/mol@LB@Unfolding Energy - sRNA 1.6427 kcal/mol@LB@@LB@Position - mRNA 53 -- 65@LB@Position - sRNA 27 -- 41@LB@Position Seed - mRNA 59 -- 65@LB@Position Seed - sRNA 27 -- 33@LB@
58	0.002968	0.1645	b0421	ispA	-9.70	0.019970	54 -- 76	25 -- 47	geranyltranstransferase	_____________53______________________77\n_____________\|_______________________\|\n5'-CUG...CCGCU_G__________GAAC_______AACGC...UCG-3'\n______________G_AAGAGGCGCU____GAGUUCG\n______________\|_\|\|\|\|:\|\|:\|\|____:\|:\|\|\|\|\n______________C_UUCUUCGUGA____UUUAAGC\n3'-UUU...GAAUU_G__________AUUU_______AAUGU...CAA-5'\n_____________\|_______________________\|\n_____________48______________________24\n	70 -- 76	25 -- 31	-22.3845 kcal/mol	6.43631 kcal/mol	6.25056 kcal/mol	53 77@LB@ \| \|@LB@5'-CUG...CCGCU G GAAC AACGC...UCG-3'@LB@ G AAGAGGCGCU GAGUUCG@LB@ \| \|\|\|\|:\|\|:\|\| :\|:\|\|\|\|@LB@ C UUCUUCGUGA UUUAAGC@LB@3'-UUU...GAAUU G AUUU AAUGU...CAA-5'@LB@ \| \|@LB@ 48 24@LB@@LB@@LB@Energy -9.70 kcal/mol@LB@Hybridization Energy -22.3845 kcal/mol@LB@Unfolding Energy - mRNA 6.43631 kcal/mol@LB@Unfolding Energy - sRNA 6.25056 kcal/mol@LB@@LB@Position - mRNA 54 -- 76@LB@Position - sRNA 25 -- 47@LB@Position Seed - mRNA 70 -- 76@LB@Position Seed - sRNA 25 -- 31@LB@
47	0.002119	0.1452	b0694	kdpE	-6.96	0.113004	177 -- 189	26 -- 38	response regulator in two-component regulatory system with KdpD	_____________176___________190\n_____________\|_____________\|\n5'-ACG...CCUGA_____________CAUGA...CGC-3'\n______________ACUUGAAGAAUUU\n______________\|\|\|\|:\|\|:\|\|\|\|:\n______________UGAAUUUUUUAAG\n3'-UUU...CUUCG_____________CAAUG...CAA-5'\n_____________\|_____________\|\n_____________39____________25\n	179 -- 185	30 -- 36	-10.6038 kcal/mol	2.21135 kcal/mol	1.43364 kcal/mol	176 190@LB@ \| \|@LB@5'-ACG...CCUGA CAUGA...CGC-3'@LB@ ACUUGAAGAAUUU@LB@ \|\|\|\|:\|\|:\|\|\|\|:@LB@ UGAAUUUUUUAAG@LB@3'-UUU...CUUCG CAAUG...CAA-5'@LB@ \| \|@LB@ 39 25@LB@@LB@@LB@Energy -6.96 kcal/mol@LB@Hybridization Energy -10.6038 kcal/mol@LB@Unfolding Energy - mRNA 2.21135 kcal/mol@LB@Unfolding Energy - sRNA 1.43364 kcal/mol@LB@@LB@Position - mRNA 177 -- 189@LB@Position - sRNA 26 -- 38@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 30 -- 36@LB@
154	0.01606	0.3359	b2587	kgtP	-4.55	0.394910	6 -- 12	52 -- 58	alpha-ketoglutarate transporter	_____________5_______13\n_____________\|_______\|\n______5'-UCCCC_______CCACU...UCG-3'\n______________AACUUGC\n______________\|\|\|\|\|\|\|\n______________UUGAACG\n3'-UUU...CUACU_______AAUUC...CAA-5'\n_____________\|_______\|\n_____________59______51\n	6 -- 12	52 -- 58	-7.82918 kcal/mol	0.15004 kcal/mol	3.13411 kcal/mol	5 13@LB@ \| \|@LB@ 5'-UCCCC CCACU...UCG-3'@LB@ AACUUGC@LB@ \|\|\|\|\|\|\|@LB@ UUGAACG@LB@3'-UUU...CUACU AAUUC...CAA-5'@LB@ \| \|@LB@ 59 51@LB@@LB@@LB@Energy -4.55 kcal/mol@LB@Hybridization Energy -7.82918 kcal/mol@LB@Unfolding Energy - mRNA 0.15004 kcal/mol@LB@Unfolding Energy - sRNA 3.13411 kcal/mol@LB@@LB@Position - mRNA 6 -- 12@LB@Position - sRNA 52 -- 58@LB@Position Seed - mRNA 6 -- 12@LB@Position Seed - sRNA 52 -- 58@LB@
124	0.01089	0.2829	b0186	ldcC	-10.51	0.011388	34 -- 66	24 -- 58	lysine decarboxylase 2 constitutive	_____________33___________________________________67\n_____________\|____________________________________\|\n5'-AAA...AUCUC___G__________CU____________________CGUUA...UUA-3'\n______________GAC_UG_UUAAGCA__GAAG_AUUUAAAAAA_UCGU\n______________:\|\|_\|\|_\|\|\|\|\|\|\|__\|\|\|\|_\|:\|\|\|\|\|\|\|\|_\|\|\|\|\n______________UUG_AC_AAUUCGU__CUUC_UGAAUUUUUU_AGCA\n3'-UUU...CUACU___A__G_______U_____G__________A____AUGUG...CAA-5'\n_____________\|____________________________________\|\n_____________59___________________________________23\n	53 -- 59	32 -- 38	-24.2219 kcal/mol	7.43557 kcal/mol	6.27608 kcal/mol	33 67@LB@ \| \|@LB@5'-AAA...AUCUC G CU CGUUA...UUA-3'@LB@ GAC UG UUAAGCA GAAG AUUUAAAAAA UCGU@LB@ :\|\| \|\| \|\|\|\|\|\|\| \|\|\|\| \|:\|\|\|\|\|\|\|\| \|\|\|\|@LB@ UUG AC AAUUCGU CUUC UGAAUUUUUU AGCA@LB@3'-UUU...CUACU A G U G A AUGUG...CAA-5'@LB@ \| \|@LB@ 59 23@LB@@LB@@LB@Energy -10.51 kcal/mol@LB@Hybridization Energy -24.2219 kcal/mol@LB@Unfolding Energy - mRNA 7.43557 kcal/mol@LB@Unfolding Energy - sRNA 6.27608 kcal/mol@LB@@LB@Position - mRNA 34 -- 66@LB@Position - sRNA 24 -- 58@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 32 -- 38@LB@
143	0.01412	0.3181	b0642	leuS	-6.82	0.122159	244 -- 266	37 -- 58	leucyl-tRNA synthetase	_____________243_____________________267\n_____________\|_______________________\|\n5'-AAU...AGUAC_____CA_____A__________ACAUU...AGA-3'\n______________AGCUU__UUGGG_UGAGAAGCGC\n______________\|:\|\|\|__\|\|::\|_::\|\|\|\|\|\|:\|\n______________UUGAA__AAUUC_GUUCUUCGUG\n3'-UUU...CUACU_____CG________________AAUUU...CAA-5'\n_____________\|_______________________\|\n_____________59______________________36\n	260 -- 266	37 -- 43	-20.1861 kcal/mol	7.11823 kcal/mol	6.24354 kcal/mol	243 267@LB@ \| \|@LB@5'-AAU...AGUAC CA A ACAUU...AGA-3'@LB@ AGCUU UUGGG UGAGAAGCGC@LB@ \|:\|\|\| \|\|::\| ::\|\|\|\|\|\|:\|@LB@ UUGAA AAUUC GUUCUUCGUG@LB@3'-UUU...CUACU CG AAUUU...CAA-5'@LB@ \| \|@LB@ 59 36@LB@@LB@@LB@Energy -6.82 kcal/mol@LB@Hybridization Energy -20.1861 kcal/mol@LB@Unfolding Energy - mRNA 7.11823 kcal/mol@LB@Unfolding Energy - sRNA 6.24354 kcal/mol@LB@@LB@Position - mRNA 244 -- 266@LB@Position - sRNA 37 -- 58@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 37 -- 43@LB@
6	4.032e-05	0.02042	b0889	lrp	-10.51	0.011361	210 -- 221	37 -- 48	leucine-responsive global transcriptional regulator	_____________209__________222\n_____________\|____________\|\n5'-GUG...UAGAU____________CCUGG...AGC-3'\n______________AGCAAGAAGCGC\n______________\|\|\|\|\|\|\|\|\|\|:\|\n______________UCGUUCUUCGUG\n3'-UUU...CGAAU____________AAUUU...CAA-5'\n_____________\|____________\|\n_____________49___________36\n	215 -- 221	37 -- 43	-19.5953 kcal/mol	3.24674 kcal/mol	5.83483 kcal/mol	209 222@LB@ \| \|@LB@5'-GUG...UAGAU CCUGG...AGC-3'@LB@ AGCAAGAAGCGC@LB@ \|\|\|\|\|\|\|\|\|\|:\|@LB@ UCGUUCUUCGUG@LB@3'-UUU...CGAAU AAUUU...CAA-5'@LB@ \| \|@LB@ 49 36@LB@@LB@@LB@Energy -10.51 kcal/mol@LB@Hybridization Energy -19.5953 kcal/mol@LB@Unfolding Energy - mRNA 3.24674 kcal/mol@LB@Unfolding Energy - sRNA 5.83483 kcal/mol@LB@@LB@Position - mRNA 210 -- 221@LB@Position - sRNA 37 -- 48@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 37 -- 43@LB@
189	0.02233	0.3796	b3417	malP	-8.63	0.040366	227 -- 245	35 -- 53	maltodextrin phosphorylase	_____________226_________________246\n_____________\|___________________\|\n5'-ACA...GAUAA__AAUUU____________UCACG...GCC-3'\n______________GC_____CAGGAAGCGCUU\n______________\|\|_____\|\|:\|\|\|\|\|:\|\|\|\n______________CG_____GUUCUUCGUGAA\n3'-UUU...UUGAA__AAUUC____________UUUUU...CAA-5'\n_____________\|___________________\|\n_____________54__________________34\n	239 -- 245	35 -- 41	-18.4249 kcal/mol	3.68713 kcal/mol	6.10399 kcal/mol	226 246@LB@ \| \|@LB@5'-ACA...GAUAA AAUUU UCACG...GCC-3'@LB@ GC CAGGAAGCGCUU@LB@ \|\| \|\|:\|\|\|\|\|:\|\|\|@LB@ CG GUUCUUCGUGAA@LB@3'-UUU...UUGAA AAUUC UUUUU...CAA-5'@LB@ \| \|@LB@ 54 34@LB@@LB@@LB@Energy -8.63 kcal/mol@LB@Hybridization Energy -18.4249 kcal/mol@LB@Unfolding Energy - mRNA 3.68713 kcal/mol@LB@Unfolding Energy - sRNA 6.10399 kcal/mol@LB@@LB@Position - mRNA 227 -- 245@LB@Position - sRNA 35 -- 53@LB@Position Seed - mRNA 239 -- 245@LB@Position Seed - sRNA 35 -- 41@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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