Input and runtime details for job 6756176 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6756176 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:22:58 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:23:17 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:01:55 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:02:03 CET 2018
	Job Completed		@ Fri Feb 16 20:02:30 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6756176&toolName=CopraRNA ( 30 days expiry )

Description of the job

DsrA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
118	0.009927	0.271	b1654	grxD	-11.37	0.006167	180 -- 241	7 -- 60	glutaredoxin-4	_____________179_______________________________________________________________242\n_____________\|_________________________________________________________________\|\n5'-AAA...GUAAA________AGG________CAAUG_____CACUAUCG_______C_A_________AUUG_____AAACC...CUG-3'\n______________GAAGUUUG___AAGCAAGA_____AGCAC________AAAAAAU_C___ACGCCAG_____CUGA\n______________\|\|\|::\|\|\|___\|\|\|\|\|\|\|\|_____\|\|\|\|\|________\|\|\|\|\|\|\|_\|___\|\|:\|\|\|\|_____\|\|\|\|\n______________CUUUGAAC___UUCGUUCU_____UCGUG________UUUUUUA_G___UGUGGUC_____GACU\n3'-UUU...CCCUA________GAA__________________AA_____________A_CAA_______CUUUA____ACACAA-5'\n_____________\|_________________________________________________________________\|\n_____________61________________________________________________________________6\n	227 -- 233	16 -- 22	-34.247 kcal/mol	9.03509 kcal/mol	13.8468 kcal/mol	179 242@LB@ \| \|@LB@5'-AAA...GUAAA AGG CAAUG CACUAUCG C A AUUG AAACC...CUG-3'@LB@ GAAGUUUG AAGCAAGA AGCAC AAAAAAU C ACGCCAG CUGA@LB@ \|\|\|::\|\|\| \|\|\|\|\|\|\|\| \|\|\|\|\| \|\|\|\|\|\|\| \| \|\|:\|\|\|\| \|\|\|\|@LB@ CUUUGAAC UUCGUUCU UCGUG UUUUUUA G UGUGGUC GACU@LB@3'-UUU...CCCUA GAA AA A CAA CUUUA ACACAA-5'@LB@ \| \|@LB@ 61 6@LB@@LB@@LB@Energy -11.37 kcal/mol@LB@Hybridization Energy -34.247 kcal/mol@LB@Unfolding Energy - mRNA 9.03509 kcal/mol@LB@Unfolding Energy - sRNA 13.8468 kcal/mol@LB@@LB@Position - mRNA 180 -- 241@LB@Position - sRNA 7 -- 60@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 16 -- 22@LB@
87	0.005861	0.217	b3083	higB	-4.01	0.492619	220 -- 233	28 -- 42	mRNA interferase toxin of the HigB-HigA toxin-antitoxin system	_____________219_____________234\n_____________\|_______________\|\n5'-GCA...UCAAA_______________GCUGC...GAU-3'\n______________AAGCAUU_GAAAGAU\n______________\|\|\|\|\|:\|_:\|\|\|:\|\|\n______________UUCGUGA_UUUUUUA\n3'-UUU...CGUUC_______A_______AGCAA...CAA-5'\n_____________\|_______________\|\n_____________43______________27\n	220 -- 226	36 -- 42	-9.16043 kcal/mol	3.51035 kcal/mol	1.63711 kcal/mol	219 234@LB@ \| \|@LB@5'-GCA...UCAAA GCUGC...GAU-3'@LB@ AAGCAUU GAAAGAU@LB@ \|\|\|\|\|:\| :\|\|\|:\|\|@LB@ UUCGUGA UUUUUUA@LB@3'-UUU...CGUUC A AGCAA...CAA-5'@LB@ \| \|@LB@ 43 27@LB@@LB@@LB@Energy -4.01 kcal/mol@LB@Hybridization Energy -9.16043 kcal/mol@LB@Unfolding Energy - mRNA 3.51035 kcal/mol@LB@Unfolding Energy - sRNA 1.63711 kcal/mol@LB@@LB@Position - mRNA 220 -- 233@LB@Position - sRNA 28 -- 42@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 36 -- 42@LB@
97	0.007216	0.239	b2021	hisC	-5.42	0.261518	59 -- 66	37 -- 44	histidinol-phosphate aminotransferase	_____________58_______67\n_____________\|________\|\n5'-GUU...UUUCC________AUGAC...GGC-3'\n______________AGAAGCGC\n______________\|\|\|\|\|\|:\|\n______________UCUUCGUG\n3'-UUU...UUCGU________AAUUU...CAA-5'\n_____________\|________\|\n_____________45_______36\n	60 -- 66	37 -- 43	-11.6623 kcal/mol	1.85203 kcal/mol	4.39141 kcal/mol	58 67@LB@ \| \|@LB@5'-GUU...UUUCC AUGAC...GGC-3'@LB@ AGAAGCGC@LB@ \|\|\|\|\|\|:\|@LB@ UCUUCGUG@LB@3'-UUU...UUCGU AAUUU...CAA-5'@LB@ \| \|@LB@ 45 36@LB@@LB@@LB@Energy -5.42 kcal/mol@LB@Hybridization Energy -11.6623 kcal/mol@LB@Unfolding Energy - mRNA 1.85203 kcal/mol@LB@Unfolding Energy - sRNA 4.39141 kcal/mol@LB@@LB@Position - mRNA 59 -- 66@LB@Position - sRNA 37 -- 44@LB@Position Seed - mRNA 60 -- 66@LB@Position Seed - sRNA 37 -- 43@LB@
56	0.002803	0.1597	b2514	hisS	-5.93	0.201249	279 -- 295	24 -- 41	histidyl tRNA synthetase	_____________278_________________296\n_____________\|___________________\|\n5'-AUG...UUGAA_____AC____________GCUCG-3'\n______________GGCAC__UGAAAAA__CGU\n______________:\|\|\|\|__\|:\|\|\|\|\|__\|\|\|\n______________UCGUG__AUUUUUU__GCA\n3'-UUU...GUUCU_____A________AA___AUGUG...CAA-5'\n_____________\|___________________\|\n_____________42__________________23\n	286 -- 292	29 -- 35	-10.1195 kcal/mol	2.54562 kcal/mol	1.64881 kcal/mol	278 296@LB@ \| \|@LB@5'-AUG...UUGAA AC GCUCG-3'@LB@ GGCAC UGAAAAA CGU@LB@ :\|\|\|\| \|:\|\|\|\|\| \|\|\|@LB@ UCGUG AUUUUUU GCA@LB@3'-UUU...GUUCU A AA AUGUG...CAA-5'@LB@ \| \|@LB@ 42 23@LB@@LB@@LB@Energy -5.93 kcal/mol@LB@Hybridization Energy -10.1195 kcal/mol@LB@Unfolding Energy - mRNA 2.54562 kcal/mol@LB@Unfolding Energy - sRNA 1.64881 kcal/mol@LB@@LB@Position - mRNA 279 -- 295@LB@Position - sRNA 24 -- 41@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 29 -- 35@LB@
2	4.936e-07	0.0007949	b1237	hns	-13.24	0.001473	195 -- 222	26 -- 57	global DNA-binding transcriptional dual regulator H-NS	_____________194______________________________223\n_____________\|________________________________\|\n5'-CUC...AGAUU___ACAA_________________________UGAAC...UAG-3'\n______________ACU____UGAGC__GAAGCACUUAAA__AUUC\n______________\|\|\|____\|:\|\|\|__\|\|\|\|\|\|\|\|\|\|\|\|__\|\|\|\|\n______________UGA____AUUCG__CUUCGUGAAUUU__UAAG\n3'-UUU...UACUU___ACGA_____UU____________UU____CAAUG...CAA-5'\n_____________\|________________________________\|\n_____________58_______________________________25\n	212 -- 218	32 -- 38	-22.6274 kcal/mol	3.10903 kcal/mol	6.27605 kcal/mol	194 223@LB@ \| \|@LB@5'-CUC...AGAUU ACAA UGAAC...UAG-3'@LB@ ACU UGAGC GAAGCACUUAAA AUUC@LB@ \|\|\| \|:\|\|\| \|\|\|\|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ UGA AUUCG CUUCGUGAAUUU UAAG@LB@3'-UUU...UACUU ACGA UU UU CAAUG...CAA-5'@LB@ \| \|@LB@ 58 25@LB@@LB@@LB@Energy -13.24 kcal/mol@LB@Hybridization Energy -22.6274 kcal/mol@LB@Unfolding Energy - mRNA 3.10903 kcal/mol@LB@Unfolding Energy - sRNA 6.27605 kcal/mol@LB@@LB@Position - mRNA 195 -- 222@LB@Position - sRNA 26 -- 57@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 32 -- 38@LB@
161	0.01751	0.3504	b3394	hofN	-4.92	0.333288	275 -- 286	18 -- 29	DNA catabolic putative fimbrial assembly protein	_____________274__________287\n_____________\|____________\|\n5'-UGG...CUGAU____________UCUGC...CCG-3'\n______________GUUCGUUGCGCC\n______________:\|\|\|\|\|\|:\|:\|\|\n______________UAAGCAAUGUGG\n3'-UUU...UUUUU____________UCCUU...CAA-5'\n_____________\|____________\|\n_____________30___________17\n	275 -- 281	23 -- 29	-17.9024 kcal/mol	4.53191 kcal/mol	8.4513 kcal/mol	274 287@LB@ \| \|@LB@5'-UGG...CUGAU UCUGC...CCG-3'@LB@ GUUCGUUGCGCC@LB@ :\|\|\|\|\|\|:\|:\|\|@LB@ UAAGCAAUGUGG@LB@3'-UUU...UUUUU UCCUU...CAA-5'@LB@ \| \|@LB@ 30 17@LB@@LB@@LB@Energy -4.92 kcal/mol@LB@Hybridization Energy -17.9024 kcal/mol@LB@Unfolding Energy - mRNA 4.53191 kcal/mol@LB@Unfolding Energy - sRNA 8.4513 kcal/mol@LB@@LB@Position - mRNA 275 -- 286@LB@Position - sRNA 18 -- 29@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 23 -- 29@LB@
99	0.007347	0.239	b4348	hsdS	-4.18	0.460078	133 -- 139	70 -- 76	specificity determinant for hsdM and hsdR	_____________132_____140\n_____________\|_______\|\n5'-CUG...GUGAA_______CGAGC...UAA-3'\n______________CUGGGGG\n______________\|\|\|:\|\|\|\n______________GACUCCC\n3'-UUU...GCUGG_______CCAGC...CAA-5'\n_____________\|_______\|\n_____________77______69\n	133 -- 139	70 -- 76	-10.3039 kcal/mol	2.51492 kcal/mol	3.60488 kcal/mol	132 140@LB@ \| \|@LB@5'-CUG...GUGAA CGAGC...UAA-3'@LB@ CUGGGGG@LB@ \|\|\|:\|\|\|@LB@ GACUCCC@LB@3'-UUU...GCUGG CCAGC...CAA-5'@LB@ \| \|@LB@ 77 69@LB@@LB@@LB@Energy -4.18 kcal/mol@LB@Hybridization Energy -10.3039 kcal/mol@LB@Unfolding Energy - mRNA 2.51492 kcal/mol@LB@Unfolding Energy - sRNA 3.60488 kcal/mol@LB@@LB@Position - mRNA 133 -- 139@LB@Position - sRNA 70 -- 76@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 70 -- 76@LB@
166	0.01873	0.3614	b0473	htpG	-6.61	0.137913	119 -- 133	26 -- 39	protein refolding molecular co-chaperone Hsp90 Hsp70-dependent; heat-shock protein; ATPase	_____________118_____________134\n_____________\|_______________\|\n5'-UCA...GCUCU_________UU____UCCCU...GUG-3'\n______________CGCUUGAAA__AUUC\n______________\|:\|\|\|:\|\|\|__\|\|\|\|\n______________GUGAAUUUU__UAAG\n3'-UUU...UCUUC_________U_____CAAUG...CAA-5'\n_____________\|_______________\|\n_____________40______________25\n	121 -- 127	31 -- 37	-10.0825 kcal/mol	1.82368 kcal/mol	1.64668 kcal/mol	118 134@LB@ \| \|@LB@5'-UCA...GCUCU UU UCCCU...GUG-3'@LB@ CGCUUGAAA AUUC@LB@ \|:\|\|\|:\|\|\| \|\|\|\|@LB@ GUGAAUUUU UAAG@LB@3'-UUU...UCUUC U CAAUG...CAA-5'@LB@ \| \|@LB@ 40 25@LB@@LB@@LB@Energy -6.61 kcal/mol@LB@Hybridization Energy -10.0825 kcal/mol@LB@Unfolding Energy - mRNA 1.82368 kcal/mol@LB@Unfolding Energy - sRNA 1.64668 kcal/mol@LB@@LB@Position - mRNA 119 -- 133@LB@Position - sRNA 26 -- 39@LB@Position Seed - mRNA 121 -- 127@LB@Position Seed - sRNA 31 -- 37@LB@
180	0.02111	0.3768	b2753	iap	-4.77	0.357003	164 -- 179	25 -- 40	aminopeptidase in alkaline phosphatase isozyme conversion	_____________163______________180\n_____________\|________________\|\n5'-UAG...CUAUG___G_A__________CAUGG...CUA-3'\n______________GCG_U_AAAAAAUUUG\n______________\|\|:_\|_\|\|\|\|\|\|\|\|:\|\n______________CGU_A_UUUUUUAAGC\n3'-UUU...UUCUU___G_A__________AAUGU...CAA-5'\n_____________\|________________\|\n_____________41_______________24\n	170 -- 176	28 -- 34	-8.71545 kcal/mol	2.29678 kcal/mol	1.64821 kcal/mol	163 180@LB@ \| \|@LB@5'-UAG...CUAUG G A CAUGG...CUA-3'@LB@ GCG U AAAAAAUUUG@LB@ \|\|: \| \|\|\|\|\|\|\|\|:\|@LB@ CGU A UUUUUUAAGC@LB@3'-UUU...UUCUU G A AAUGU...CAA-5'@LB@ \| \|@LB@ 41 24@LB@@LB@@LB@Energy -4.77 kcal/mol@LB@Hybridization Energy -8.71545 kcal/mol@LB@Unfolding Energy - mRNA 2.29678 kcal/mol@LB@Unfolding Energy - sRNA 1.64821 kcal/mol@LB@@LB@Position - mRNA 164 -- 179@LB@Position - sRNA 25 -- 40@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 28 -- 34@LB@
11	0.0001294	0.03789	b3687	ibpA	-8.25	0.051487	69 -- 81	26 -- 38	heat shock chaperone	_____________68____________82\n_____________\|_____________\|\n5'-AAC...AUAAG_____________AUUUC...GCU-3'\n______________GCUUGAAAAGUUC\n______________:\|\|\|:\|\|\|\|:\|\|\|\n______________UGAAUUUUUUAAG\n3'-UUU...CUUCG_____________CAAUG...CAA-5'\n_____________\|_____________\|\n_____________39____________25\n	75 -- 81	26 -- 32	-13.0918 kcal/mol	3.40592 kcal/mol	1.43364 kcal/mol	68 82@LB@ \| \|@LB@5'-AAC...AUAAG AUUUC...GCU-3'@LB@ GCUUGAAAAGUUC@LB@ :\|\|\|:\|\|\|\|:\|\|\|@LB@ UGAAUUUUUUAAG@LB@3'-UUU...CUUCG CAAUG...CAA-5'@LB@ \| \|@LB@ 39 25@LB@@LB@@LB@Energy -8.25 kcal/mol@LB@Hybridization Energy -13.0918 kcal/mol@LB@Unfolding Energy - mRNA 3.40592 kcal/mol@LB@Unfolding Energy - sRNA 1.43364 kcal/mol@LB@@LB@Position - mRNA 69 -- 81@LB@Position - sRNA 26 -- 38@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 26 -- 32@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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